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A protocol for structured illumination microscopy with minimal reconstruction artifacts

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Biophysics Reports 2019年第2期5卷 80-90页

作者： Junchao Fan Xiaoshuai Huang Liuju Li Shan Tan Liangyi Chen Key Laboratory of Image Processing and Intelligent Control of Ministry of Education of China School of Automation Huazhong University of Science and Technology State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular Medicine Peking University

The imaging rate of structured illumination microscopy(SIM) reached 188 Hz recently. As the exposure time decreases, the camera detects fewer virtual photons, while the noise level remains the same. As a result, the signal-to-noise ratio(SNR) decreases sharply. Furthermore, the SNR decreases further because of photobleaching and phototoxicity. This decreased quality of SIM raw data may lead to surprising artifacts with various causes, which may confuse a new user of SIM microscopy. We summarize three significant possible sources of severe artifacts in reconstructed super-resolution(SR)images. Ultrafast motion of a biological sample or an uneven illumination pattern is the most difficult to be identified. The estimated parameter could also be incorrect, leading to artifact of regular ***, reconstruction with the Wiener method generates stochastic artifacts due to the amplification of noise during the deconvolution process. To deal with these problems, we have established a protocol to reconstruct ultrafast SIM raw data obtained in low SNR conditions. First, we checked the quality of the raw data with the image J plugin SIMcheck before reconstruction. Then, a modified parameter estimation method was used to improve the precision of the parameters. Finally, an iterative algorithm was used for SIM reconstruction under low signal-to-noise ratio conditions. This procedure effectively suppressed the artifacts in the super-resolution images reconstructed from raw data of low signal-to-noise ratio.

关键词： Ultrafast SIM Low signal-to-noise ratio Reconstruction

Adaptive Fuzzy Tracking Control with Global Prescribed-Time Prescribed Performance for Uncertain Strict-Feedback Nonlinear Systems

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arXiv 2022年

作者： Mao, Bing Wu, Xiaoqun Liu, Hui Xu, Yuhua Lü, Jinhu The School of Mathematics and Statistics Wuhan University Hubei430072 China Hubei Key Laboratory of Computational Science Wuhan University Hubei430072 China Research Center of Complex Network Wuhan University Hubei430072 China The School of Automation Huazhong University of Science and Technology Hubei430074 China The Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China Hubei430074 China The School of Statistics and Data Science Nanjing Audit University Jiangsu211815 China The School of Automation Science and Electrical Engineering Beihang University Beijing100191 China

Adaptive fuzzy control strategies are established to achieve global prescribed performance with prescribed-time convergence for strict-feedback systems with mismatched uncertainties and unknown nonlinearities. Firstly, to quantify the transient and steady performance constraints of the tracking error, a class of prescribed-time prescribed performance functions are designed, and a novel error transformation function is introduced to remove the initial value constraints and solve the singularity problem in existing works. Secondly, based on dynamic surface control methods, controllers with or without approximating structures are established to guarantee that the tracking error achieves prescribed transient performance and converges into a prescribed bounded set within prescribed time. In particular, the settling time and initial value of the prescribed performance function are completely independent of initial conditions of the tracking error and system parameters, which improves existing results. Moreover, with a novel Lyapunov-like energy function, not only the differential explosion problem frequently occurring in backstepping techniques is solved, but the drawback of the semi-global boundedness of tracking error induced by dynamic surface control can be overcome. The validity and effectiveness of the main results are verified by numerical simulations on practical examples. Copyright © 2022, The Authors. All rights reserved.

关键词： Fuzzy control

Path Planning Algorithm for Unmanned Surface Vessel Based on Multiobjective Reinforcement Learning

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Computational intelligence and neuroscience 2023年第1期2023卷 2146314页

作者： Caipei Yang Yingqi Zhao Xuan Cai Wei Wei Xingxing Feng Kaibo Zhou MOE Key Laboratory of Image Information Processing and Intelligent Control School of Artificial Intelligence and Automation Huazhong University of Science and Technology Wuhan 430074 China. Wuhan Second Ship Design and Research Institute Wuhan 430205 China.

It is challenging to perform path planning tasks in complex marine environments as the unmanned surface vessel approaches the goal while avoiding obstacles. However, the conflict between the two subtarget tasks of obstacle avoidance and goal approaching makes the path planning difficult. Thus, a path planning method for unmanned surface vessel based on multiobjective reinforcement learning is proposed under the complex environment with high randomness and multiple dynamic obstacles. Firstly, the path planning scene is set as the main scene, and the two subtarget scenes including obstacle avoidance and goal approaching are divided from it. The action selection strategy in each subtarget scene is trained through the double deep -network with prioritized experience replay. A multiobjective reinforcement learning framework based on ensemble learning is further designed for policy integration in the main scene. Finally, by selecting the strategy from subtarget scenes in the designed framework, an optimized action selection strategy is trained and used for the action decision of the agent in the main scene. Compared with traditional value-based reinforcement learning methods, the proposed method achieves a 93% success rate in path planning in simulation scenes. Furthermore, the average length of the paths planned by the proposed method is 3.28% and 1.97% shorter than that of PER-DDQN and dueling DQN, respectively.

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Exploiting learnable joint groups for hand pose estimation

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arXiv 2020年

作者： Li, Moran Gao, Yuan Sang, Nong Key Laboratory of Image Processing and Intelligent Control School of Artificial Intelligence and Automation Huazhong University of Science and Technology Wuhan China Tencent AI Lab United States

In this paper, we propose to estimate 3D hand pose by recovering the 3D coordinates of joints in a group-wise manner, where less-related joints are automatically categorized into different groups and exhibit different features. This is different from the previous methods where all the joints are considered holistically and share the same feature. The benefits of our method are illustrated by the principle of multi-task learning (MTL), i.e., by separating less-related joints into different groups (as different tasks), our method learns different features for each of them, therefore efficiently avoids the negative transfer (among less related tasks/groups of joints). The key of our method is a novel binary selector that automatically selects related joints into the same group. We implement such a selector with binary values stochastically sampled from a Concrete distribution, which is constructed using Gumbel softmax on trainable parameters. This enables us to preserve the differentiable property of the whole network. We further exploit features from those less-related groups by carrying out an additional feature fusing scheme among them, to learn more discriminative features. This is realized by implementing multiple 1x1 convolutions on the concatenated features, where each joint group contains a unique 1x1 convolution for feature fusion. The detailed ablation analysis and the extensive experiments on several benchmark datasets demonstrate the promising performance of the proposed method over the state-of-the-art (SOTA) methods. Besides, our method achieves top-1 among all the methods that do not exploit the dense 3D shape labels on the most recently released FreiHAND competition at the submission date. The source code and models are available at https://***/moranli-aca/LearnableGroups-Hand. Copyright © 2020, The Authors. All rights reserved.

关键词： Convolution

Research on Public Opinion Guidance Strategy based on Interaction Constraints

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Research on Public Opinion Guidance Strategy based on Intera...

IEEE Information Technology and Mechatronics Engineering Conference (ITOEC)

作者： Yuanyuan Xu Zhan Wu Xi Chen School of Artificial Intelligence and Automation Huazhong University of Science and Technology Wuhan China Image Processing and Intelligent Control Key Laboratory of the Education Ministry of China Wuhan China

ISBN: (数字)9781728143231

ISBN: (纸本)9781728143248

The dissemination of public opinion has a considerable impact on people's social lives, and the question of how both the government and the media can correctly guide public opinion and restore social stability is an issue that requires urgent attention. Combining the characteristics of public opinion dissemination in the network society leads to individual interaction. Considering the interaction between individual views and network topology, this paper combines the collaborative network model with the distance-constrained Hegselmann-Krause (HK) model to propose a consensus-guided strategy based on interaction constraints. It does this by changing the network topology and limiting the scope of individual interactions, thereby affecting the spread of public opinion. Based on the small-world network, the collaborative network model introduces the two parameters of Connection edge probability p and broken edge probability q, while also proposing a collaborative network model for Connection edge's breaking of the network based on the difference of individual opinion values. This paper designs simulation experiments by changing p, q, and the interaction distance, while also analyzing the results of public opinion's evolution from the perspectives of timeliness and the diversity of viewpoints. The experimental results show that when individuals are restricted from interacting with the surrounding environment and the scope of interaction between individuals is restricted, this will slow down the evolution of public opinion and increase the number of opinion clusters. By contrast, when it is encouraged, the interaction between individuals and the surrounding environment will make the entire system communicate more effectively within a short period of time, promoting the evolutionary benefits of public opinion considerably, shortening the convergence time of public opinion evolution to reach a steady state, and reducing the number of opinion clusters. The strategy is consist

关键词： Network topology Collaborative work Social network services Economics Government Bridges Media

Probabilistic Charging Power Forecast of EVCS: Reinforcement Learning Assisted Deep Learning Approach

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arXiv 2022年

作者： Li, Yuanzheng He, Shangyang Li, Yang Ge, Leijiao Lou, Suhua Zeng, Zhigang School of Artificial Intelligence and Automation Key Laboratory on Image Information Processing and Intelligent Control of Ministry of Education Huazhong University of Science and Technology Wuhan430074 China China-Belt and Road Joint Laboratory on Measurement and Control Technology Wuhan430074 China China-EU Institute for Clean and Renewable Energy Huazhong University of Science and Technology Wuhan430074 China School of Electrical Engineering Northeast Electric Power University Jilin 132012 China The Key Laboratory of Smart Grid of Ministry of Education Tianjin University Tianjin 300072 China School of Electrical and Electronic Engineering Huazhong University of Science and Technology Wuhan430074 China

The electric vehicle (EV) and electric vehicle charging station (EVCS) have been widely deployed with the development of large-scale transportation electrifications. However, since charging behaviors of EVs show large uncertainties, the forecasting of EVCS charging power is non-trivial. This paper tackles this issue by proposing a reinforcement learning assisted deep learning framework for the probabilistic EVCS charging power forecasting to capture its uncertainties. Since the EVCS charging power data are not standard time-series data like electricity load, they are first converted to the time-series format. On this basis, one of the most popular deep learning models, the long short-term memory (LSTM) is used and trained to obtain the point forecast of EVCS charging power. To further capture the forecast uncertainty, a Markov decision process (MDP) is employed to model the change of LSTM cell states, which is solved by our proposed adaptive exploration proximal policy optimization (AePPO) algorithm based on reinforcement learning. Finally, experiments are carried out on the real EVCSs charging data from Caltech, and Jet Propulsion laboratory, USA, respectively. The results and comparative analysis verify the effectiveness and outperformance of our proposed framework. Copyright © 2022, The Authors. All rights reserved.

关键词： Markov processes

Context prior for scene segmentation

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arXiv 2020年

作者： Yu, Changqian Wang, Jingbo Gao, Changxin Yu, Gang Shen, Chunhua Sang, Nong Key Laboratory of Image Processing and Intelligent Control School of Artificial Intelligence and Automation Huazhong University of Science and Technology University of Adelaide Australia Chinese University of Hong Kong Tencent

Recent works have widely explored the contextual dependencies to achieve more accurate segmentation results. However, most approaches rarely distinguish different types of contextual dependencies, which may pollute the scene understanding. In this work, we directly supervise the feature aggregation to distinguish the intra-class and inter-class context clearly. Specifically, we develop a Context Prior with the supervision of the Affinity Loss. Given an input image and corresponding ground truth, Affinity Loss constructs an ideal affinity map to supervise the learning of Context Prior. The learned Context Prior extracts the pixels belonging to the same category, while the reversed prior focuses on the pixels of different classes. Embedded into a conventional deep CNN, the proposed Context Prior Layer can selectively capture the intra-class and inter-class contextual dependencies, leading to robust feature representation. To validate the effectiveness, we design an effective Context Prior Network (CPNet). Extensive quantitative and qualitative evaluations demonstrate that the proposed model performs favorably against state-of-the-art semantic segmentation approaches. More specifically, our algorithm achieves 46.3% mIoU on ADE20K, 53.9% mIoU on PASCAL-Context, and 81.3% mIoU on Cityscapes. Code is available at https://***/ContextPrior. Copyright © 2020, The Authors. All rights reserved.

关键词： Pixels

Multi-Wavelength Narrow Linewidth Random Fiber Laser Based on Fiber Bragg Grating Fabry-Perot Filter

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Multi-Wavelength Narrow Linewidth Random Fiber Laser Based o...

International Conference on Information Science and Technology (ICIST)

作者： Sokliep Pheng Luo Xiaonan Zhongshuai Wang Yijie Zhu Zetao Jiang School of Information and Communication Guilin University of Electronic Technology Guilin China Guangxi Key Laboratory of Intelligent Processing of Computer Image and Graphics Guilin University of Electronic Technology Guilin China School of Computer Science and Information Security Guilin University of Electronic Technology Guilin China

ISBN: (数字)9781728155586

ISBN: (纸本)9781728155593

Recently, multi-wavelength narrow linewidth random fiber laser has very interested for every researcher in this field, because of their useful advantages application, such as high-resolution spectroscopy and fiber optic sensing. In this paper, the standard single-mode fiber is used to form a half-opened cavity structure for generating the narrow linewidth random fiber laser and used the FBG-FP as a filter to form the narrow linewidth RFL into multi-wavelength. Firstly, we used the Rayleigh scattering that processed as well as in a standard single-mode fiber to provide random distribution feedback at the same time while using erbium-doped fiber (EDF) to provide the gain or amplification for achieving a broadband random laser output. Then, FBG-FP is added to the half-open cavity random laser structure. The multi-wavelength and narrow linewidth RFL can be achieved when the broadband RFL goes through the FBG-FP. In this paper we have generated the multi-wavelength narrow linewidth random fiber laser which has more than 10 wavelengths and the 3dB bandwidth is less than 0.01 nm and the mode separation of each wavelength is 0.04nm.

关键词： Fiber gratings Erbium-doped fiber lasers Optical fiber couplers Optical fiber amplifiers

GTNet: Generative transfer network for zero-shot object detection

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arXiv 2020年

作者： Zhao, Shizhen Gao, Changxin Shao, Yuanjie Li, Lerenhan Yu, Changqian Ji, Zhong Sang, Nong Key Laboratory of Ministry of Education for Image Processing and Intelligent Control School of Artificial Intelligence and Automation Huazhong University of Science and Technology School of Electrical and Information Engineering Tianjin University

We propose a Generative Transfer Network (GTNet) for zero-shot object detection (ZSD). GTNet consists of an Object Detection Module and a Knowledge Transfer Module. The Object Detection Module can learn large-scale seen domain knowledge. The Knowledge Transfer Module leverages a feature synthesizer to generate unseen class features, which are applied to train a new classification layer for the Object Detection Module. In order to synthesize features for each unseen class with both the intra-class variance and the IoU variance, we design an IoU-Aware Generative Adversarial Network (IoUGAN) as the feature synthesizer, which can be easily integrated into GTNet. Specifically, IoUGAN consists of three unit models: Class Feature Generating Unit (CFU), Foreground Feature Generating Unit (FFU), and Background Feature Generating Unit (BFU). CFU generates unseen features with the intra-class variance conditioned on the class semantic embeddings. FFU and BFU add the IoU variance to the results of CFU, yielding class-specific foreground and background features, respectively. We evaluate our method on three public datasets and the results demonstrate that our method performs favorably against the state-of-the-art ZSD approaches. Copyright © 2020, The Authors. All rights reserved.

关键词： Object recognition