Skeletal muscle has a robust regeneration ability that is impaired by severe injury,disease,and *** in a decline in skeletal muscle ***,improving skeletal muscle regeneration is a key challenge in treating skeletal mu...
Skeletal muscle has a robust regeneration ability that is impaired by severe injury,disease,and *** in a decline in skeletal muscle ***,improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related *** to their significant role in tissue regeneration,implantation of M2 macrophages(M2M?) has great potential for improving skeletal muscle ***,we present a short-wave infrared(SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2M? *** fluorescence imaging was employed to track implanted M2M? in the injured skeletal muscle of mouse *** is found that the implanted M2M? accumulated at the injury site for two ***,SWIR fluorescence imaging of blood vessels showed that M2M? implantation could improve the relative perfusion ratio on day 5(1.09±0.09 vs 0.85±0.05;p=0.01) and day 9(1.38±0.16 vs 0.95±0.03;p=0.01) post-injury,as well as augment the degree of skeletal muscle regencration on day 13 ***,multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle *** results provide more in vivo details about M2M? in skeletal muscle regeneration and confirm that M2M? could promote angiogenesis and improve the degree of skeletal muscle repair,which will guide the research and development of M2M? implantation to improve skeletal muscle regeneration.
Lipid peroxidation (LPO) is one of the most damaging processes in chemodynamic therapy (CDT). Although it is well known that polyunsaturated fatty acids (PUFAs) are much more susceptible than saturated or monounsatura...
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Lipid peroxidation (LPO) is one of the most damaging processes in chemodynamic therapy (CDT). Although it is well known that polyunsaturated fatty acids (PUFAs) are much more susceptible than saturated or monounsaturated ones to LPO, there is no study exploring the effect of cell membrane unsaturation degree on CDT. Here, we report a self-reinforcing CDT agent (denoted as OA@Fe-SAC@EM NPs), consisting of oleanolic acid (OA)-loaded iron single-atom catalyst (Fe-SAC)-embedded hollow carbon nanospheres encapsulated by an erythrocyte membrane (EM), which promotes LPO to improve chemodynamic efficacy via modulating the degree of membrane unsaturation. Upon uptake of OA@Fe-SAC@EM NPs by cancer cells, Fe-SAC-catalyzed conversion of endogenous hydrogen peroxide into hydroxyl radicals, in addition to initiating the chemodynamic therapeutic process, causes the dissociation of the EM shell and the ensuing release of OA that can enrich cellular membranes with PUFAs, enabling LPO amplification-enhanced CDT.
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