检索结果-内蒙古大学图书馆

The chromosome-scale genome of black wolfberry(Lycium ruthenicum)provides useful genomic resources for identifying genes related to anthocyanin biosynthesis and disease resistance

引用

Plant Diversity 2025年第2期47卷 201-213页

作者： Gulbar Yisilam Enting Zheng Chuanning Li Zhiyong Zhang Ying Su Zhenzhou Chu Pan Li Xinmin Tian Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Ministry of Education)&Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin Guangxi Normal UniversityGuilin 541006China Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and TechnologyXinjiang UniversityUrumqi 830046China Laboratory of Systematic&Evolutionary Botany and Biodiversity College of Life SciencesZhejiang UniversityHangzhou 310058China Guangxi University Enginering Research Center of Bioinformation and Genetic Improvement of Speciaty Crops Guangxi Normal UniversityGuilin 541006China

The black wolfberry(Lycium ruthenicum;2n=2x=24)is an important medicinal plant with ecological and economic *** fruits have numerous beneficial pharmacological activities,especially those of anthocyanins,polysaccharides,and alkaloids,and have high nutritional ***,the lack of available genomic resources for this species has hindered research on its medicinal and evolutionary *** this study,we developed the telomere-to-telomere(T2T)nearly gapless genome of ***(2.26 Gb)by integrating PacBio HiFi,Nanopore Ultra-Long,and Hi-C *** assembled genome comprised 12 chromosomes with 37,149 protein-coding genes functionally *** 80%of the repetitive sequences were identified,of which long terminal repeats(LTRs)were the most abundant,accounting for 73.01%.The abundance of LTRs might be the main reason for the larger genome of this species compared to that of other Lycium *** species-specific genes of *** were related to defense mechanisms,salt tolerance,drought resistance,and oxidative stress,further demonstrating their superior adaptability to arid *** on the assembled genome and fruit transcriptome data,we further constructed an anthocyanin biosynthesis pathway and identified 19 candidate structural genes and seven transcription factors that regulate anthocyanin biosynthesis in the fruit developmental stage of ***,most of which were highly expressed at a later stage in fruit ***,154 potential disease resistance-related nucleotidebinding genes have been identified in the *** *** whole-genome and proximal,dispersed,and tandem duplication genes in the *** genome enriched the number of genes involved in anthocyanin synthesis and resistance-related *** results provide an important genetic basis for understanding genome evolution and biosynthesis of pharmacologically active components in the Lycium genus.

关键词： Lycium ruthenicum Genome Anthocyanin biosynthesis Gene duplication Comparative genomics

来源：评论

学校读者我要写书评

暂无评论

Preliminary study of the mechanism of isolinderalactone inhibiting the malignant behavior of bladder cancer

引用

Current Urology 2025年第1期19卷 49-58页

作者： Qun Wang Wenkai Xu Lu Ying Hongjin Shi Yuxin Sun Wei Feng Haole Xu Jun Xie Hairong Wei Zhao Yang Haifeng Wang Department of Urology The Second Affiliated Hospital of Kunming Medical UniversityKunmingChina Department of Urology The Second People's Hospital of Xindu DistrictChengduChina College of Life Science and Technology Innovation Center of Molecular DiagnosticsBeijing University of Chemical TechnologyBeijingChina College of Life Science and Technology Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction CorpsTarim UniversityAlarXinjiangChina

Background:Isolinderalactone(ILL),extracted from the dried tubers of Linderae aggregate,has multiple functions,such as antioxidation,antitumor,and anti-infection ***,there have been fewstudies on ILL's antitumor role and no reports on its role in bladder cancer(BC).Materials and methods:Human BC cell lines T24 and EJ-1 were treated with different concentrations of ILL(0,10,20,50,100,200,400,600,800,and 1000μmol/L),and the cell proliferation inhibition rate was analyzed using the CCK-8 *** effect of ILL on T24 and EJ-1 cell cycle inhibition and apoptosis was examined using flow *** was used to detect the levels of apoptosis-related proteins,BAX,BAK1,and CYCS,in BC cells of the control and ILL-treated groups,and quantitative PCR experiments were performed to detect the apoptosis-related gene expression of CASP10,CYCS,BAX,BCL-2,CASP8,and BAK1.T24 and EJ-1 tumor-bearingmousemodels were established and divided into vehicle control,low-dose(10mg/kg)and high-dose(20mg/kg)groups,with 5 mice in each *** and eosin staining and immunohistochemistry were used to detect changes in apoptosisrelated proteins in ***:The CCK-8 assay showed that in vitro,ILL significantly inhibited the proliferation of the T24 and EJ-1 BC cell *** and immunoblotting results showed that ILL increased mitochondrial permeability by upregulating proapoptotic proteins BAK1 and BAX,promoting CYCS release and significantly inducing cell cycle arrest at G0/G1 *** vivo,on day 25 of administration,tumor inhibition rates in T24 and EJ-1 tumor-bearing mice were up to 75.24% and 47.43%,respectively,in the ILL high-dose-treated and 71.58% and 43.89%,respectively,in the ILL low-dose-treated ***:Isolinderalactone controls BC progression by inducing apoptosis,suggesting that ILL may be an effective drug for the treatment of BC.

关键词： Bladder cancer Isolinderalactone Mitochondrial apoptosis

来源：评论

学校读者我要写书评

暂无评论

Design and synthesis of thiolutin derived PSMD14/HDAC dual-target inhibitors against esophageal squamous cell carcinoma

引用

Bioorganic Chemistry 2025年 161卷 108500页

作者： He, Guoguo Wu, Zhenhui Yang, Xuan Luo, Xinrong Zhang, Lili Du, Zhenting Li, Shuwei Wan, Chuanxing Key Laboratory of Conservation and Utilization of Biological Resources in the Tarim Basin Alaer Xinjiang 843300 China College of Life Science and Technology Tarim University Alaer Xinjiang 843300 China College of Chemical Pharmacy Northwest A&F University Shaanxi Yangling 712100 China

Esophageal cancer is one of the most migratory, invasive, and lethal malignancies and has a poor prognosis, highlighting the urgent need to develop more effective drugs for its treatment. Given that PSMD14 and HDAC play an important role in the treatment of esophageal cancer, thiolutin is used as a lead compound to design and synthesize a series of dual-target PSMD14/HDAC small molecule inhibitors, aiming to discover more effective anti-esophageal cancer drugs. Through the in vitro screening of PSMD14/HDAC enzyme inhibitory activities of a series of thiolutin derivatives, it was found that compound 8b, with a linker length of 8 and a Zn²⁺-chelating group of 1,2-phenylenediamine, exhibited the most balanced inhibitory activity against PSMD14/*** impact of 8b on PSMD14/HDAC at the cellular level was evaluated, and its drug-like properties were further assessed in vivo. Compound 8b demonstrates balanced dual-target activity (PSMD14 IC₅₀ = 238.7 ± 27 nM, HDAC1 IC₅₀ = 141.2 ± 10.3 nM) and excellent cytotoxicity against esophageal cancer cells (IC₅₀ = 30–250 nM), effectively reversing epithelial-mesenchymal transition in cancer cells. Moreover, 8b exhibited excellent pharmacokinetic characteristics. More importantly, in a nude mouse xenograft model with subcutaneous transplantation of KYSE 30 cells, compound 8b (0.8 mg/kg, BID, PO, TGI = 81 %;0.8 mg/kg, Q3D, SC, TGI = 77 %) significantly inhibited tumor growth, outperforming single-agent or combination treatments, thereby highlighting the therapeutic advantages of dual-target inhibition. These findings highlight the potential of dual-target PSMD14/HDAC inhibitors as a promising strategy for developing anti-esophageal cancer drugs. © 2025

关键词： Drug discovery EMT Esophageal cancer PSMD14 / HDAC dual-inhibitors Thiolutin derivatives

来源：评论

学校读者我要写书评

暂无评论

Comparative analysis of chloroplast genomes and phylogenetic relationships of different pitaya cultivars

引用

BMC Genomics 2025年第1期26卷 1-16页

作者： Zheng, Enting Yisilam, Gulbar Li, Chuanning Jiao, Fangfang Ling, Yulan Lu, Shuhua Wang, Qiuyan Tian, Xinmin Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education) & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin Guangxi Normal University Guilin 541006 China Guangxi University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops Guangxi Normal University Guilin 541006 China Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology Xinjiang University Urumqi 830046 China Guangxi Institute of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences Guilin 541006 China

Background: Pitaya is an important tropical fruit highly favoured by consumers owing to its good and juicy characteristics. It contains a large amount of betacyanin, which is a natural food-colouring agent, in the peel and pulp. However, few studies have focused on the pitaya chloroplast (cp) genomes. Results: To explore the genetic differences and phylogenetic relationships among the cp genomes of the six pitaya cultivars, we assembled, annotated, and performed a comparative genomic analysis. The cp genomes of the six cultivars exhibited a typical circular structure, ranging in length from 133,146 to 133,617 bp, with a GC content of 36.4%. All individual cp genomes were annotated with 123 genes, including 80 protein-coding genes, 38 tRNA genes, four rRNA genes, and one pseudogene (ycf68). Six mutated hotspot regions (trnF-GAA-rbcL, trnM-CAU-accD, rpl20-psbB, accD, rpl22, ycf1) were detected, which could be considered potential molecular markers for population genetics and molecular phylogeny studies. Phylogenetic analysis showed that pitaya cultivars clustered into a single branch in the phylogenetic tree of the Cactaceae family. Furthermore, the observed phylogenetic patterns suggest a complex genetic basis for colour variation among pitaya cultivars. Conclusions: The study findings expand our understanding of the cp genome of pitaya and the phylogenetic relationships among different cultivars. The genomic data obtained provide important information for the breeding and genetic improvement of pitaya. © The Author(s) 2025.

关键词： Chloroplast genome Phylogenetic analysis Pitaya Structural characteristics

来源：评论

学校读者我要写书评

暂无评论

Genome-wide analysis of apple histone acetyltransferases reveals the regulatory roles of MdHAG1 and MdHAM1 in response to abiotic stresses

引用

Plant Science 2025年 355卷 112441页

作者： Jiang, Weiyu Hong, Furong Niu, Bolin Zhu, Hongzhen Yang, Mengyao Yan, Jinjiao Wang, Jiangbo Song, Xiaolin Ma, Fengwang Guan, Qingmei Zheng, Qianming Xu, Jidi State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of Horticulture Northwest A&F University Shaanxi Yangling 712100 China Institute of Pomology Science Guizhou Academy of Agricultural Science Guiyang 550006 China The National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in Southern Xinjiang College of Horticulture and Forestry Tarim University Alar 843300 China Xinjiang Production and Construction Corps Key Laboratory of Biological Resources Protection and Utilization in Tarim Basin Alar 843300 China

It is known that histone acetyltransferases (HATs) are involved in a wide range of biological processes by activating gene expression. However, the regulatory role of HATs in apple remains to be elucidated. This study identified 58 HATs from the apple genome (named MdHATs) and performed comprehensive analyses of these MdHATs, given their involvements in plant development and stress response. Firstly, we classified 58 predicted MdHATs into four different categories based on the phylogenetic analyses. Then, the intron/exon structures, conserved motifs, and structural domain organization of MdHAT genes and predicted proteins were further analyzed. Next, we investigated the expression patterns of MdHATs during apple plants' development process and stress responses. Moreover, according to these findings, we selected two candidate genes, MdHAG1 and MdHAM1, and ectopically expressed them in tobacco to investigate their function in response to drought and low-temperature stress, respectively. The results showed that overexpression of MdHAG1 in tobacco negatively regulated drought tolerance and cold tolerance of transgenic tobacco, and overexpression of MdHAM1 in tobacco improved drought tolerance and cold tolerance of transgenic tobacco. In summary, our study provides a comprehensive analysis of the MdHAT family and insights into the epigenetic mechanisms of histone acetylases under abiotic stress. © 2025 Elsevier B.V.

关键词： Abiotic stresses Apple Epigenetics Histone acetyltransferase (HAT) Phenotypic analysis

来源：评论

学校读者我要写书评

暂无评论

Genetic parameter estimation and molecular foundation of Double-yolk eggs trait in white leghorn

引用

Poultry Science 2025年第7期104卷 105069页

作者： Chen, Anqi Zhao, Xiaoyu Wang, Haiyan Zhao, Xiurong Wang, Gang Zhang, Xinye Ren, Xufang Zhang, Yalan Cheng, Xue Yu, Xiaofan Mei, Xiaohan Wang, Huie Guo, Menghan Jiang, Xiaoyu Zhang, Fuping Ning, Zhonghua Qu, Lujiang National Engineering Laboratory for Animal Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 China Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Tarim University Alar 843300 China Key Laboratory of Animal Genetics Breeding and Reproduction in the Plateau Mountainous Region Ministry of Education College of Animal Sciences Guizhou University Guizhou Province Guiyang 550025 China Xingrui Agricultural Stock Breeding Hebei Province Baoding 072550 China Hohhot Customs District Inner Mongolia Autonomous Region Hohhot 010010 China

Double-yolk (DY) eggs refer to the presence of two yolks in an egg, and they are often present in fowl flocks. As abnormal eggs, DY eggs occur frequently in the early stages of egg-laying in hens, as well as in hens with early sexual maturity. In order to understand the inheritant pattern of DY eggs and explore candidate genes associated with DY eggs, we selected over 10,000 white Leghorn (WL) chickens from 4 generations and recorded the data during the egg laying period, including total egg number and the rate of DY egg number during the first 2 months and the period of 18-58 weeks of age (EN2month, EN38, EN58, DY2month, DY38, and DY58), age at first egg (AFE), first egg weight (FEW), and body weight at first egg (BWA). The results of genetic parameter analysis showed that the DY egg rate was a trait with low to medium heritabilities with the values from 0.15 to 0.29. And there were strong positive phenotypic and genetic correlations between DY egg rate and egg production at different age stages, and they were all strongly negatively correlated with AFE. However, the DY egg rate and egg production at different stages had strong positive and negative genetic correlations with BWA and FEW, respectively. We also found that significant differences in these trait values between different generations and cage layers, indicating that generations and cage layers had a certain influence on these traits. Furtherly, we used whole genome-wide association (GWA) analysis to identify genes underlying DY, and 5 candidate genes (EZH2, CNTNAP2, TMEM163, GPC1, and ACMSD) associated with DY2month in WL. Our study improved the understanding of DY eggs in hens, and the genetic parameters of DY eggs, and also provided insights into reducing the production of DY eggs by various selection strategies. © 2025

关键词： Double-yolk eggs Genome-wide association study Heritability White Leghorn

来源：评论

学校读者我要写书评

暂无评论

Complete chloroplast genome sequence ofKarelinia caspia(Pall.) Less. (Compositae)

引用

Mitochondrial DNA Part B 2025年第1期10卷

作者： Wenjuan Huang Shuangfei Song Chengzhi Peng Hongyan Jin Peipei Jiao Zhihua Wu a Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin College of Life Science Tarim University Alar PR China b College of Life Sciences Zhejiang Normal University Jinhua PR China

Karelinia caspia(Compositae) is a perennial herbaceous plant owning high economic, feeding and medicinal values. It is widely distributed in desertification and saline alkali areas. The complete chloroplast genome was firstly reported in this study. The chloroplast genome of K. caspia with a total size of 151,239 bp consists of two inverted repeats separated by a large single-copy region and a small single-copy region. Its chloroplast genome contains 129 genes, including 85 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. Also, a total of 62 simple sequence repeats were identified. These results will be useful for study on the evolution and genetic diversity of K. caspia in the future.

关键词： Karelinia caspia (Pall.) Less. Compositae chloroplast genome phylogeny

来源：评论

学校读者我要写书评

暂无评论

Morphological and physiological differences in heteromorphic leaves of male and female Populus euphratica Oliv.

引用

Journal of Arid Land 2022年第12期14卷 1456-1469页

作者： LI Xiu ZHAI Juntuan LI Zhijun College of Life Sciences and Technology Tarim UniversityAlar 843300China Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production&Construction Corps Alar 843300China

Leaf traits can directly reflect the adaptation strategies of plants to the ***,there is limited knowledge on the adaptation strategies of heteromorphic leaves of male and female Populus euphratica *** response to individual developmental stages(i.e.,diameter class)and canopy height *** this study,morphological and physiological properties of heteromorphic leaves of male and female *** were *** showed that both male and female *** exhibited increased leaf area(LA),leaf dry weight(LDW),leaf thickness(LT),net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(g_(s)),proline(Pro),and malondialdehyde(MDA)concentration,decreased leaf shape index(LI)and specific leaf area(SLA)with increasing diameter and canopy *** water potential(LWP)increased with increasing diameter,LWP decreased significantly with increasing canopy height in both sexes,and carbon isotope fraction(δ^(13)C)increased significantly with canopy height in both sexes,all of which showed obvious resistance ***,males showed greater LA,LT,P_(n),T_(r),and Pro than females at the same canopy height,and males showed significantly higher LA,SLA,LT,P_(n),T_(r),g_(s),and MDA,but lower LWP and δ_(1)3C than females at the same canopy height,suggesting that male *** have stronger photosynthetic and osmoregulatory abilities,and are sensitive to water ***,difference between male and female *** is closely related to the increase in individual diameter class and canopy *** summary,male plants showed higher stress tolerance than female plants,and differences in P_(n),g_(s),T_(r),Pro,MDA,δ_(13)C,and LWP between females and males were related to changes in leaf morphology,diameter class,and canopy *** results of this study provide a theory for the differences in growth adaptation strategies during individual development of ***.

关键词： arid area canopy height dioecious plants morphology physiological characteristics

来源：评论

学校读者我要写书评

暂无评论

Effects of temperature on flowering phenological traits of Populus euphratica Oliv. and Populus pruinosa Schrenk populations, Xinjiang, China

引用

Journal of Arid Land 2019年第5期11卷 754-763页

作者： LI Zhijun ZHANG Xiao ZHENG Yaqiong QIU Aijun ZHANG Ling College of Life Sciences Tarim UniversityAral 843300China Key Laboratory of Protection and Utilization of Biological Resource in Tarim Basin Xinjiang Production&Construction GroupsAral 843300China

The aims of this study were to explore the interspecific differences of Populus euphratica *** Populus pruinosa Schrenk populations and the intraspecific differences of males and females within the same species in flowering phenological traits,and the effects of temperatures on flowering phenological traits in different growth years(2001–2003 and 2013–2015).The results showed that *** population flowered earlier than *** ***,flowering phenological period of population,number of days of flowering phenological period per population,number of days of flowering phenological period per plant and average number of days of flowering period per plant of *** population were less than those of *** *** differences between male and female within the same species indicated that the flowering periods of males *** and *** populations were earlier than those of female *** both species,flowering phenological traits were significantly and negatively correlated with the average temperatures in previous ten days,previous one month and previous three months of *** species are sensitive to temperature changes and adjust to the changes by advancing the start of flowering and prolonging the duration of flowering.

关键词： Populus euphratica Oliv. Populus pruinosa Schrenk flowering phenology temperature flowering synchrony

来源：评论

学校读者我要写书评

暂无评论

Dssimilatory Fe(Ⅲ) reduction characteristics of paddy soil extract cultures treated with glucose or fatty acids

引用

环境科学学报（英文版） 2008年第9期20卷 1103-1108页

作者： HE Jiangzhou QU Dong College of Resources and Environment Northwest A & F University Yangling Shaanxi 712100 China Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Bioiogical Resources in Tarim Basin Tarim University Alar Xinjian College of Resources and Environment Northwest A & F University Yangling Shaanxi 712100 China

Dissimilatory Fe(Ⅲ) reduction is a universal process with irreplaceable biological and environmental importance in anoxic environments. Our knowledge about Fe(Ⅲ) reduction predominantly comes from pure cultures of dissimilatory Fe(Ⅲ) reducing bacteria (DFRB). The objective of this study was to compare the effects of glucose and a selection of short organic acids (citrate, succinate, pyruvate, propionate, acetate, and formate) on Fe(Ⅲ) reduction via the anaerobic culture of three paddy soil solutions with Fe(OH)3 as the sole electron acceptor. The results showed significant differences in Fe(Ⅲ) reduction among the three paddy soil solutions and suhstrate types. Bacteria from the Sichuan paddy soil responded quickly to substrate supply and showed higher Fe(Ⅲ) reducing activity than the other two soil types. Bacteria in the Jiangxi paddy soil culture solution could not use propionate as a source of electrons for Fe(Ⅲ) reduction. Similarly, bacteria in the Jilin paddy soil culture could not use succinate effectively. Pyruvate was readily used by cultures from all three paddy soil solutions, thus indicating that there were some similarities in substrate utilization by bacteria for Fe(Ⅲ) reduction. The use of glucose and citrate as substrate for dissimilatory Fe(Ⅲ) reduction indicates important ecological implications for this type of anoxic respiration.

关键词： dissimilatory Fe(Ⅲ) reduction glucose organic acid anaerobic incubation

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：