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Chronic Effects of Benzalkonium Chlorides on Short Chain Fatty Acids and Methane Production in Semi-Continuous Anaerobic Digestion of Waste Activated Sludge

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SSRN 2022年

作者： Yang, Chun-Xue He, Zhang-Wei Liu, Wen-Zong Wang, Ai-Jie Wang, Ling Liu, Jia Liu, Bao-Ling Ren, Nan-Qi Yu, Shao-Peng Guo, Ze-Chong Heilongjiang Province Key Laboratory of Cold Region Wetland Ecology and Environment Research School of Geography and Tourism Harbin University Harbin China National Technology Innovation Center of Synthetic Biology Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China Shanxi Key Laboratory of Environmental Engineering Key Laboratory of Northwest Water Resource Environment and Ecology MOE School of Environmental and Municipal Engineering Xi’an University of Architecture and Technology Xi’an China State Key Laboratory of Urban Water Resource and Environment Shenzhen Key Laboratory of Organic Pollution Prevention and Control Harbin Institute of Technology Shenzhen Shenzhen China School of Environmental & Municipal Engineering Qingdao University of Technology Qingdao China School of Environmental and Chemical Engineering Jiangsu University of Science and Technology Zhenjiang China

Benzalkonium chlorides (BACs) are one kind of emerging pollutants in wastewater and potentially enriched in waste activated sludge (WAS) because of its quick adsorption but slow degradation under anaerobic condition, which will impact acidogenesis and methanogenesis in anaerobic digestion (AD). It is worthy to well understand the microbial response mechanism under chronic effects of BACs for resource recovery from WAS. This study investigated the AD (by-)products and microbial evolution under low to high BACs concentrations from bioreactor startup to steady running. It was found that BACs can lead to an increase of WAS hydrolysis and fermentation, but a disturbance to acidogenic bacteria also occurred at low BACs concentration, while a noticeable inhibition to methanogenesis occurred when BAC concentration was up to 15 mg/g TSS. The analysis of metabolic pathways showed that BACs shifted the metabolism of short chain fatty acids (SCFAs), and the improved the propionic acid (HPr) reduction process, while the relative abundance of Clostridium and Longilinea were increased. Furthermore, the functional species involved in methane metabolism had succeeded from aceticlastic methanogens to hydrogenotrophic methanogens, while the abundance of coding genes in converged stage was reduced by BACs, which finally led to a decline in methane production. © 2022, The Authors. All rights reserved.

关键词： Methane

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Selective Inhibition of Different Inhibitors in Coal Slurry Flotation and Kinetic Simulation Studies

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SSRN 2024年

作者： Niu, Fusheng Zhang, Jinxia Wang, Ziye Gao, Shuling He, Shengtao Yang, Chao College of Mining Engineering North China University of Science and Technology Tangshan063210 China GRINM Resources and Environment Tech.Co Ltd Beijing100000 China Hebei Key Laboratory of Mining Development and Safety Technology Tangshan063210 China College of Resources and Civil Engineering Northeastern University Shenyang11089 China

The interactions of dextrin, sodium hexametaphosphate，and sodium silicate with three minerals (kaolinite, quartz，and coal samples) were investigated by a combination of molecular dynamics simulation and experimental verification. According to the simulation calculations, dextrin and the three minerals were mainly physically adsorbed, and the results of the interaction energy calculations showed that dextrin had the strongest interaction with kaolinite, followed by quartz, and the weakest interaction with coal samples. Sodium hexametaphosphate forms O-Al and O-Si ionic bonds with kaolinite (001), and Na-O ionic bonds with quartz (101). According to the results of the interaction energy calculation, it is concluded that sodium hexametaphosphate has the strongest interaction with kaolinite, followed by quartz, and the weakest in coal samples. During the interaction between sodium silicate and kaolinite (001) surface, O in silicate root interacts with Si and Al in kaolinite in the form of an ionic bond. Sodium silicate solution forms Na-O ionic structures during interaction with quartz. The electrostatic potential energy and non-bonding energy of sodium silicate solution dominate in the interaction process with coal samples. According to the interaction energy calculation, the interaction strength of sodium silicate solution with three minerals is kaolinite, quartz and coal samples in order. Comparing the results of the interaction energies of the three inhibitors with the three minerals, it can be concluded that the best inhibitor for selective inhibition of veinstone minerals is sodium silicate, followed by dextrin. Meanwhile, the molecular simulation of dextrin-sodium silicate (1:1) interaction with the three minerals showed that the synergistic effect of the two inhibitors was greater than that of a single inhibitor. When the dosage of composite inhibitor is 1000g/t, the coal slurry can be sorted to obtain the flotation coal product with an ash content of 10.69% and

关键词： Molecular dynamics

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The diversity and ecological significance of microbial traits potentially involved in B_(12) biosynthesis in the global ocean

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mLife 2023年第4期2卷 416-427页

作者： Jiayin Zhou Wei Qin Xinda Lu Yunfeng Yang David Stahl Nianzhi Jiao Jizhong Zhou Jihua Liu Qichao Tu Institute of Marine Science and Technology Shandong UniversityQingdaoChina Joint Lab for Ocean Research and Education at Dalhousie University Shandong University and Xiamen UniversityQingdaoChina School of Biological Sciences University of OklahomaNormanOklahomaUSA Department of Civil and Environmental Engineering Massachusetts Institute of TechnologyCambridgeMassachusettsUSA State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijingChina Department of Civil and Environmental Engineering University of WashingtonSeattleWashingtonUSA Institute of Marine Microbes and Ecospheres Xiamen UniversityXiamenChina Earth and Environmental Sciences Lawrence Berkeley NationalLaboratoryBerkeley CaliforniaUSA Institutefr Environmental Genomics University of OklahomaNormanOklahomaUSA School of Civil Engineering and Environmental Sciences University of OklahomaNormanOklahomaUSA School of Computer Sciences University of OklahomaNormanOklahomaUSA DermBiont Inc. BostonMassachusettsUSA

Cobalamin(B_(12)),an essential nutrient and growth cofactor for many living organisms on Earth,can be fully synthesized only by selected prokaryotes in ***,microbial communities related to B_(12) biosynthesis could serve as an example subsystem to disentangle the underlying ecological mechanisms balancing the function and taxonomic make-up of complex functional *** anchoring microbial traits potentially involved in B_(12) biosynthesis,we depict the biogeographic patterns of B_(12) biosynthesis genes and the taxa harboring them in the global ocean,despite the limitations of detecting de novo B_(12) synthesizers via metagenomes *** the taxonomic and functional composition of B_(12) biosynthesis genes were strongly shaped by depth,differentiating the epipelagic zones from the mesopelagic *** genes related to B_(12) biosynthesis were relatively stably distributed across different oceans,but the taxa harboring them varied considerably,showing clear functional redundancy among microbial *** taxa carrying B_(12) biosynthesis genes in the surface water were influenced by environmental factors such as temperature,oxygen,and ***,the composition of functional genes was only weakly associated with these environmental *** model analyses demonstrated that determinism governed the variations in B_(12) biosynthesis genes,whereas a higher degree of stochasticity was associated with taxonomic *** associations were observed between the chlorophyll a concentration and B_(12) biosynthesis,confirming its importance in primary production in the global *** results of this study reveal an essential ecological mechanism governing the assembly of microbes in nature:the environment selects for function rather than taxonomy;functional redundancy underlies stochastic community assembly.

关键词： B_(12)biosynthesis community assembly functional genes functional redundancy ocean primary production

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Threshold of Sediment Incipient Motion on a Three-Dimensional Riverbed by Considering the Hiding-Exposure Effect with Experimental Investigations

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SSRN 2024年

作者： Li, Linlin Xiao, Yao Zhang, Genguang Lei, Yu Li, Hang Liu, Defu School of Civil Engineering Architecture and Environment Hubei University of Technology Wuhan430068 China College of Water Resource and Architectural Engineering Northwest A&F University Yangling712100 China Key Laboratory of Intelligent Health Perception and Ecological Restoration of Rivers and Lakes Ministry of Education Hubei University of Technology Wuhan430068 China

The initial position of bed sediment particles is one of the key factors affecting the sediment incipient motion in the coastal erosion and riverbed evolution;however, the current modes of sediment incipient motion were simplified by considering the sediment particles as a two-dimensional "pancake". Therefore, the probability density functions (PDFs) of relative exposure degree and exposure angle of the surface sediment particles under three incipient modes on a three-dimensional (3D) riverbed were investigated by the self-designed test device;and on this basis, a new formula of sediment incipient motion velocity on a 3D riverbed was proposed by considering the hiding-exposure effect, and then the validity of the proposed formula was investigated by comparing with the flume test data, field measurements and other formulae. The results show that: (1) the relative exposure degree of uniform sediment varies from 0 to 0.5, with a mean relative exposure degree of 0.185, 0.196 and 0.2 for three incipient modes, respectively, and its PDF approximately obeys the normal distribution. (2) the exposure angle of sphere particles in the range of [0, 90°], with the average exposure angle of 49°, 34° and 49.6° for three incipient modes, respectively, and the PDF of sediment exposure angle also obeys the normal distribution;(3) the proposed formula of sediment motion verified by the flume test data, field measurements and other classical formulae, and it shows a higher accuracy with the measured data, with a relative error of less than 1.3%, which is smaller than other formulae. In addition, the influence of flow intensity, sediment exposure angle and particle Reynolds number on the sediment incipient motion were investigated;and it was found that the incipient flow velocity increased with the increasing exposure angle, but the sediment incipient probability decreased with the increasing exposure angle. This study has a wide application to the local scouring process in the downstre

关键词： Probability density function

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Spatiotemporal Patterns and Synergy of the environmental Rebound Effect: Evidence from Carbon Emissions and Pm2.5 Pollution in China

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SSRN 2024年

作者： Han, Xiao Wang, Yichen Du, Mingxi Tian, Chenguang Xie, Meiying Zhang, Linru Jiang, Yingkun School of Public Policy and Administration Xi’an Jiaotong University 28 Xianning Road West Shaanxi Xi’an710049 China Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Collaborative Innovation Center of Atmospheric Environment and Equipment Technology School of Environmental Science and Engineering Nanjing University of Information Science and Technology Jiangsu Nanjing210044 China Xi’an Institute for Innovative Earth Environment Research Shaanxi Xi’an710061 China

technology progress is one of the most attractive measures to tackling the dual pressures of carbon reduction and fine particulate matter (PM2.5) pollution control. However, due to the existence of the environmental rebound effect (ERE), such technical efforts are limited. The spatiotemporal patterns and synergy of the ERE for carbon and PM2.5 emissions have not been thoroughly explored, thereby hindering the realization of co-abatement strategies. This study employs the growth accounting approach to estimate the ERE across 30 provinces in China from 2001 to 2019. The average magnitude of the rebound effect for carbon emissions (CRE) reached 125.45%, while that for PM2.5 pollution (PRE) was 45.01%. CRE and PRE substantially offset the potential savings, particularly the backfire effect of carbon emissions. The degrees of both CRE and PRE experienced a generally diminishing trend over the studied period, with significant heterogeneity in the spatial distribution pattern. Additionally, we find that the synergistic reduction effect between CRE and PRE was approximately 0.97, and that the synergy grades of the eastern coastal as well as central regions were higher than that of the western region. These findings enhance the understanding of the intertwined relationship between carbon and PM2.5 reductions from the perspective of the ERE, providing crucial references for collaborative governance and the further achievement of sustainable development goals. © 2024, The Authors. All rights reserved.

关键词： Carbon

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Microplastics inhabited crop growth and exacerbated antibiotic resistance gene occurrence

Microplastics inhabited crop growth and exacerbated antibiot...

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第四届全国（海洋）环境微塑料污染与管控学术研讨会

作者： Min Li Mengying Shao Liuqingqing Liu Hao Zheng Institute of Coastal Environmental Pollution Control College of Environmental Science and Engineering Sanya Oceanographic Institution Ministry of Education Key Laboratory of Marine Environment and Ecology Ocean University of China Marine Ecology and Environmental Science Laboratory Pilot National Laboratory for Marine Science and Technology

Agricultural soils are important reservoirs of both antibiotic resistance genes(ARGs)and microplastics(MPs), two types of emerging pollutants. However, the effects and mechanisms of different types of MPs on the growth of crops and abundances of ARGs in agricultural soils remains unclear. Therefore, we investigated the effects of five types of common soil MPs(PA, PP,PS, PVC, and TP) on corn and cucumber seedling growth and soil ARG abundance using a 43-day microcosmic experiment. Our results showed that the five different types of MPs altered germination strategies of seeds and inhibited the seedling growth and root development for maize and cucumber by 12.2–43.3% and 29.0–42.5%. For maize, the decrease in fresh weight followed an order of PP>PVC≈PS>TP≈PA. For cucumber, little difference was observed among the different MPs. More importantly, MPs significantly increased the absolute abundance of ARGs by65.1–122% and 57.8–67.3% in the soils planted with corn and cucumber, respectively. MPs decreased soil pH, EC, moisture, and nutrient availability, thus inhibiting the water uptake and nutrient acquisition of plant. In addition, MPs changed soil microbial community structure,especially increasing ARGs potential host bacteria. Notably, the release of heavy metals and other organic additives from MPs probably enhanced the abundance of soil mobile genetic elements(MGEs), which in turn promoted horizontal transfer of ARGs. These results deepen our understanding of the ecological risk of MPs and ARGs in agricultural soil ecosystems and highlights the demand for the appropriate management strategies to mitigating the pollution of MPs and ARGs.

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Polystyrene nanoplastics promoted extracellular antibiotic resistance gene propagation through transformation

Polystyrene nanoplastics promoted extracellular antibiotic r...

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第四届全国（海洋）环境微塑料污染与管控学术研讨会

作者： Xiaohan Ma Mengying Shao Hao Zheng Institute of Coastal Environmental Pollution Control College of Environmental Science and Engineering Sanya Oceanographic Institution Ministry of Education Key Laboratory of Marine Environment and Ecology Ocean University of China Marine Ecology and Environmental Science Laboratory Pilot National Laboratory for Marine Science and Technology

Dissemination and persistence of antibiotic resistance genes(ARGs) has been considered one of the most significant emerging environmental and health challenges in the 21st century. Mariculture environments have become one of the major reservoirs of environmental ARGs, which can be spread among different bacterial strains through horizontal gene transfer(HGT) process. Although studies revealed that microplastics can serve as hot-spots for HGT of ARGs, it is still unclear how nanoplastics influence the horizontal transfer of extracellular *** therefore explored the impact of polystyrene(PS) nanoplastics on the transformation of plasmid pBR322 carrying tet-A and bla TEM-1 as a representative extracellular ARGs to Escherichia coli DH5α. PS nanoplastics at 0.1–20 mg/L significantly enhanced the transformation frequency of tet-A and bla TEM-1 into Escherichia coli DH5α by 5–24% and 18–37%, respectively, indicating PS nanoplastics promoted extracellular ARGs propagation through transformation. This was mainly attributed to the increased intracellular reactive oxygen species production, enhanced cell membrane permeability, and promoted energy driving force, thus enhancing the entrance of plasmid pBR322 into Escherichia coli DH5α. Moreover, PS nanoplastics had no obvious effect on the structure and replication capacities of plasmid pBR322. These findings provide insights regarding the contributions and the underlying mechanisms of nanoplastics to the dissemination of extracellular ARGs, and are helpful for the evaluation of the public health and environmental and ecological risks caused by nanoplastics.

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Suppression and excitation condition of collision on instabilities of electrostatic plasmas

arXiv

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arXiv 2024年

作者： Hou, Y.W. Yu, M.Y. Wang, J.F. Liu, C.Y. Chen, M.X. Wu, B. School of Mathematics & Physics Key Laboratory of Architectural Acoustic Environment of Anhui Higher Education Institutes Key Laboratory of Advanced Electronic Materials and Devices Anhui Jianzhu University Anhui Hefei230601 China CAS Key Laboratory of Geospace Environment Department of Plasma Physics & Fusion Engineering University of Science and Technology of China Anhui Hefei230026 China Shenzhen Technology University Guangdong Shenzhen518118 China Institute for Theoretical Physics I Ruhr University BochumD-44780 Germany Institute of Plasma Physics Chinese Academy of Sciences Anhui Hefei230031 China School of Physics Hefei University of Technology Anhui Hefei230009 China

Two-stream (TS) and Bump-On-Tail (BOT) electron distributions can induce instabilities in collisionless plasmas, which is closely related to phenomena in space and fusion plasmas. Collisions can lead to unexpected plasma behavior, especially in dense and/or low temperature plasmas. In this work, the Vlasov-Poisson system with Krook collisions are used to study the effect of collisions. Normally, the collision can dissipate the system energy which causes the suppression of the instabilities. Against the traditional suppression effect of collision on the instability, it is found in our simulation that the collision can also excite the instability even in the forbidden beam velocity range predicted by the cold-beam theory. With collision, the beam velocity range can be divided into suppression area [vth/2, vc + vth], transition area [vc − vth, vc + vth], excitation area [vc + vth, 2vc] and forbidden area [2vc, +∞] for TS instability. where vc is the critical velocity from cold-beam theory and vth is thermal velocity or the beam width in our simulation. The collision dissipation effect and the excitation of beam instability can compete with each other, which evoked the excitation of collision on TS instability. The collision can change the suppression and excitation condition from beam theory. However, for BOT instability, there is only suppression effect of collision on the instability. These results can expand the view of collision effect on instability of electrostatic plasmas. © 2024, CC BY-NC-SA.

关键词： Temperature

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Microbial-assistance and chelation-support techniques promoting phytoremediation under abiotic stresses

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Chemosphere 2024年 365卷 143397页

作者： Naz, Misbah Afzal, Muhammad Rahil Qi, Shan Shan Dai, Zhicong Sun, Qiuyang Du, Daolin School of Emergency Management School of the Environment and Safety Engineering Jiangsu University 301 Xuefu Road ZhenjiangJiangsu Province212013 China Institute of Environment and Ecology School of the Environment and Safety Engineering Jiangsu University 301 Xuefu Road ZhenjiangJiangsu Province212013 China Key Laboratory of Modern Agricultural Equipment and Technology Ministry of Education School of Agricultural Engineering Jiangsu University 301 Xuefu Road ZhenjiangJiangsu Province212013 China Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment Suzhou University of Science and Technology 99 Xuefu Road SuzhouJiangsu Province215009 China Jingjiang College Jiangsu University Zhenjiang212013 China

Phytoremediation, the use of plants to remove heavy metals from polluted environments, has been extensively studied. However, abiotic stresses such as drought, salt, and high temperatures can limit plant growth and metal uptake, reducing phytoremediation efficiency. High levels of HMs are also toxic to plants, further decreasing phytoremediation efficacy. This manuscript explores the potential of microbial-assisted and chelation-supported approaches to improve phytoremediation under abiotic stress conditions. Microbial assistance involves the use of specific microbes, including fungi that can produce siderophores. Siderophores bind essential metal ions, increasing their solubility and bioavailability for plant uptake. Chelation-supported methods employ organic acids and amino acids to enhance soil absorption and supply of essential metal ions. These chelating agents bind HMs ions, reducing their toxicity to plants and enabling plants to better withstand abiotic stresses like drought and salinity. Managed microbial-assisted and chelation-supported approaches offer more efficient and sustainable phytoremediation by promoting plant growth, metal uptake, and mitigating the effects of heavy metal and abiotic stresses. Managed microbial-assisted and chelation-supported approaches offer more efficient and sustainable phytoremediation by promoting plant growth, metal uptake, and mitigating the effects of HMs and abiotic *** strategies represent a significant advancement in phytoremediation technology, potentially expanding its applicability to more challenging environmental conditions. In this review, we examined how microbial-assisted and chelation-supported techniques can enhance phytoremediation a method that uses plants to remove heavy metals from contaminated sites. These approaches not only boost plant growth and metal uptake but also alleviate the toxic effects of HMs and abiotic stresses like drought and salinity. By doing so, they make phytoremediation a

关键词： Plant diseases

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Machine Learning-Guided Biochar Design for Antibiotics Adsorption

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SSRN 2023年

作者： Li, Jie Pan, Lanjia Huang, Yahui Liu, Xuejiao Ye, Zhi-Long Wang, Yin CAS Key Laboratory of Urban Pollutant Conversion Institute of Urban Environment Chinese Academy of Sciences Xiamen361021 China CAS Engineering Laboratory for Recycling Technology of Municipal Solid Wastes Xiamen361021 China Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control CAS Haixi Industrial Technology Innovation Center in Beilun Ningbo315830 China

Biochar is widely applied as an adsorbent for removing contaminants, while it is still challenging to wisely design biochar for a specific purpose. Herein, two machine learning (ML) models for biochar preparation and adsorption application were trained and combined by developing a hybrid ML-based optimization framework for biochar design based on application requirement. The test determination coefficient (R2) for predicting specific surface area (SSA), total volume and adsorption capacity of biochar were0.85, 0.88, and 0.97 with RMSE of 63.10 m2/g, 0.07 cm3/g, and 65.72 umol/g, respectively. Moreover, biochar SSA was the most important property for its adsorption capacity;and pyrolysis temperature and feedstock ash content were the top two important factors affecting SSA. Optimization results indicated that screened feedstock under optimal pyrolysis conditions can tailor desired biochar for enhancing antibiotics adsorption. This work presents an emerging and intelligent strategy to design biochar with specific physicochemical characteristics for adsorbing given pollutants. © 2023, The Authors. All rights reserved.

关键词： Pyrolysis

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