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Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis

Molecular Plant 2024年第12期17卷 1820-1832页

作者： Sumei Liu Haijun Wu Zhong Zhao MOE Key Laboratory for Cellular Dynamics Hefei National Research Center for Physical Sciences at the MicroscaleThe First Affiliated Hospital of University of Science and Technology of ChinaSchool of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations Gansu Province Key Laboratory of Gene Editing for BreedingSchool of Life SciencesLanzhou UniversityLanzhou 730000China

The plasticity of stem cells in response to environmental change is critical for multicellular ***,we show that MYB3R-like directly activates the key plant stem-cell regulator WUSCHEL(WUS)by recruiting the methyltransferase ROOT INITIATION DEFECTIVE 2(RID2),which functions in m7G methylation of the 5′cap of WUS mRNA to protect it from *** and molecular analyses showed that protein-folding genes are repressed by WUS to maintain precise protein synthesis in stem cells by preventing the reuse of misfolded ***,we found that upon heat stress,the MYB3R-like/RID2 module is repressed to reduce WUS transcript abundance through decapping of nascent WUS *** releases the inhibition of protein-folding capacity in stem cells and protects them from heat shock by eliminating misfolded protein *** together,our results reveal a strategic trade-off whereby plants reduce the accuracy of protein synthesis in exchange for the survival of stem cells at high temperatures.

关键词： WUSCHEL decapping MYB3R-like methyltransferase thermotolerance

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Recent advances in near infrared(NIR)electrochemiluminescence luminophores

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Chinese Chemical Letters 2024年第11期35卷 16-24页

作者： Yuyang Zhou Ziwang Mao Jing-Juan Xu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China School of Chemistry and Life Sciences Suzhou University of Science and TechnologySuzhou 215009China

Electrochemiluminescence has been developed as a robust analytical technique owing to its intrinsic advantages,such as near-zero background signal noise,wide dynamic ranges,high sensitivity and low cost and simple *** luminophore as the critical component to generate light signals plays significant roles in this robust analytical *** with traditional ECL luminophores,near infrared(NIR)ECL luminophores have attracted significant attentions recently due to their negligible autofluorescence,lower background interference and deep tissue *** substantial progresses have been achieved in exploring novel NIR ECL luminophores and elucidating their roles in addressing diverse challenges,there is still scarce of comprehensive reviews on the development of NIR ECL luminophores so *** this review,the recent advancements on NIR ECL materials,including inorganic metal complexes,organic small molecules,metal nanoclusters,quantum dots and lanthanide-based materials,have been thoroughly summarized and *** addition,we also provide a comprehensive overview of the challenges and prospects that lie ahead for the future development of NIR ECL luminophores in the future.

关键词： Electrochemiluminescence NIR Luminophore Nanocluster Metal complex

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Facile formation of Mg-containing interphase on nanosilicon from Agar/MgCO3 precursor for lithium-ion battery anodes

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Progress in Natural science:Materials International 2025年第1期35卷 177-186页

作者： Ke Zhang Jianhua Zhou Haoran Liu Xiaoyang Wang Jie Gao Jun-Liang Chen Haiqing Qin Wenping Liu Xiaoxu Lei Lei Miao Guangxi Key Laboratory of Information Materials Guangxi Collaborative Innovation Center of Structure and Property for New Energy Materials School of Material Science and Engineering Guilin University of Electronic Technology Novel Battery Materials Research Center of Engineering Technology State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite StructuresSchool of Physical Science and Technology Guangxi University Guangxi Key Laboratory of Superhard Material Guangxi Technology Innovation Center for Special Mineral Material China Nonferrous Metal (Guilin) Geology and Mining Co. Ltd.

The ultra-high theoretical capacity(4200 mAh g-1) of Silicon anode materials for lithium-ion batteries while which is one of the ideal replacement materials for graphite anodes. However, the poor electrical conductivity and greatly reduces the cycle life of the battery of Silicon material, which suffers from severe volume expansion during charge/discharge cycling leading to electrode pulverization. In this work, Agar/magnesium carbonate-coated silicon nanocomposites were synthesized by utilizing the "self-solidifying" property of agar. The magnesium oxide formed by the decomposition of magnesium carbonate at high temperatures mitigates the volume expansion of nanosilicon together with the derived carbon layer. Moreover, the lithium-magnesium alloy electronconducting interface layer generated by the reaction between lithium ions and magnesium oxide, which greatly shortens the diffusion length of lithium ions and electrons inside the electrode during the charge-discharge cycle. The first discharge specific capacity was 2060.2 mAh g-1, the Coulombic efficiency as high as 86.28 %, and still possesses a reversible specific capacity of 801.5 mAh g-1after cycling 200 cycles at a high current of 500 mA g-1of Agar/magnesium@Si with optimal ratio composite. The composite material used in this work largely suppresses the volume expansion of the silicon anode and contributes to the design of low-cost and high Coulombic efficiency lithium-ion batteries.

关键词： Agar-coated Lithium-ion battery Lithium-magnesium conductive layer Silicon anode

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Effect of electromagnetic stirrer centered outside the mold on steel flow field of bloom continuous casting

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China Foundry 2023年第5期20卷 461-468页

作者： Li-dong Xing Yan-ping Bao Min Wang Yi-hong Li State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing(USTB)Beijing 100083China Technical Support Center for Prevention and Control of Disastrous Accidents in Metal Smelting University of Science and Technology Beijing(USTB)Beijing 100083China School of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China

Mold electromagnetic stirring technology for optimizing the flow of molten steel is now widely used in the continuous casting production process of high-quality ***,studies on the case that the center of the electromagnetic stirrer is located outside the mold have not been ***,the effect of the electromagnetic stirrer centered outside the mold on the steel flow field was studied in detail by means of numerical simulations.A Gauss meter was used to measure the magnetic induction intensity at different positions,currents,and *** test results were compared with the simulation results of electromagnetic stirring to calibrate and verify the accuracy of the electromagnetic ***,electromagnetic force was introduced into the flow field model as a source term to study its effect on the flow field under anomalous *** results show that when the center of the electromagnetic stirrer is located outside the mold,the magnetic field strength is about twice as strong as that located inside the *** also leads to an increase in the flow velocity near the electromagnetic *** a result,the optimal electromagnetic stirring parameters(200 A,2.5 Hz)were specified when the center of the electromagnetic stirrer is located outside the mold.

关键词： numerical modeling electromagnetic stirring fluid flow outside the mold bloom

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Copper-based nanodots as efficient biomimetic antibiotics for the treatment of oral bacterial infections Author links open overlay panel

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Particuology 2024年第8期91卷 190-201页

作者： Ying Li Nan Tang Lingling Zhao Xingfu Bao Yuzhuo Wang Zhanxin Wang Min Hu Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling School and Hospital of StomatologyJilin UniversityChangchunChina Beijing Advanced Innovation Center for Soft Matter Science and Engineering College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingChina

Oral infectious diseases caused by a variety of pathogenic bacteria seriously affect the quality of ***,these diseases remain a clinical challenge because of the lack of simple,safe,and universal *** address these limitations,we synthesize CuO_(x)nanodots(CuO_(x)NDs)with excellent Fenton-like reaction activity and utilize them in the treatment of oral bacterial *** from other complicated approaches,CuO_(x)NDs are rationally prepared using a facile one-pot aqueous *** the presence of H_(2)O_(2),these well-developed CuO_(x)NDs can efficiently catalyze the generation of hydroxyl radicals(·OH)around oral pathogens,leading to the death of various ***,results of biosafety indicate the high biocompatibility and extremely low toxicity of these CuO_(x)*** understanding the admirable in vitro antibacterial effect of CuO_(x)NDs in the presence of H_(2)O_(2),we further explore their in vivo antibacterial performance on several classical animal models including oral mucosal wound model,intragingival bacteria-infected model,and the periodontal infection *** expected,these CuO_(x)NDs with wide-spectrum antibacterial activity can serve as high-performance antibacterial reagents for the treatment of various oral bacterial infections with the help of H_(2)O_(2).In brief,current nanoplatform can act as efficient antibiotics against oral pathogens with broadening the biomedical applications of copper-based nanomaterials.

关键词： Antibacterial treatment Oral diseases Fenton-likecatalytic reaction Reactive oxygen species

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Studying large biomolecules as sedimented solutes with solid-state NMR

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Biophysics Reports 2024年第4期10卷 201-212页

作者： Fan Shi Tong Zhang Juan Li Chaowei Shi Shengqi Xiang MOE Key Lab for Cellular Dynamics School of Life Sciences Division of Life Sciences and Medicine University of Science and Technology of China Hefei National Laboratory for Physical Sciences at Microscale University of Science and Technology of China

Sedimentation solid-state NMR is a novel method for sample preparation in solid-state NMR（ssNMR）studies. It involves the sedimentation of soluble macromolecules such as large protein complexes. By utilizing ultra-high centrifugal forces, the molecules in solution are driven into a high-concentrated hydrogel, resulting in a sample suitable for solid-state NMR. This technique has the advantage of avoiding the need for chemical treatment, thus minimizing the loss of sample biological activity. Sediment ssNMR has been successfully applied to a variety of non-crystalline protein solids, significantly expanding the scope of solid-state NMR research. In theory, using this method, any biological macromolecule in solution can be transferred into a sedimented solute appropriate for solid-state NMR analysis. However, specialized equipment and careful handling are essential for effectively collecting and loading the sedimented solids to solid-state NMR rotors. To improve efficiency, we have designed a series of loading tools to achieve the loading process from the solution to the rotor in one step. In this paper, we illustrate the sample preparation process of sediment NMR using the H1.4-NCP167complex, which consists of linker histone H1.4 and nucleosome core particle, as an example.

关键词： Sedimentation NMR Solid-state NMR Ultracentrifugation Magic angle spinning Nucleosome

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G-quadruplex is involved in the regulation of BmSGF1 expression in the Silkworm, Bombyx mori

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Insect science 2024年第5期31卷 1440-1452页

作者： Yanfei Chen Jin Li Kangkang Niu Yuling Peng Qisheng Song Qili Feng School of Biology and Agriculture Shaoguan UniversityShaoguanChina Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science Technology School of Life SciencesSouth China Normal UniversityGuangzhouChina and 4Division of Plant Science&TechnologyUniversityofMissouriColumbiaMissouriUSA

Advanced DNA structures,such as the G-quadruplex(G4)and the i-motif,are widely but not randomly present in the genomes of many organisms.A G4 structure was identified in the promoter of the silk gland factor-1 gene(SGFI),which is the main regulatory gene for silk production in Bombyx *** this study,a BmSGF1 G4^(-/-)ho-mozygous mutant was generated with the G4 sequence knocked *** promoter activity of BmSGF1 was lowered in the BmSGF1 G4^(-/-)***(PDS)stabilized the G4 structure and increased the promoter activity of BmSGF1,whereas anti-sense oligonu-cleotide(ASO)complementary to the G4 sequence suppressed the promoter activity of *** with wild-type larvae,the deletion of the BmSGF1 G4 structure de-creased both the expression of BmSGF1 and the fibroin heavy chain gene BmFib-H in the posterior silk gland and the weight of the ***,these results suggest that the promoter G4 structure of BmSGFI participates in the transcription regulation of the BmSGFl gene in the silkworm.

关键词： BmSGF1 G4 Bombyx mori CRISPR/Cas9 silk production

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Catalytic recycling of plastics into value-added products

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Nano Research 2024年第11期17卷 9428-9445页

作者： Tianyu Wei Pengcheng Zhou Wenxian Liu Xijun Liu Tairong Kuang Functional Polymers and Advanced Materials (FPAM) Lab Zhejiang Key Laboratory of Plastic Modification and Processing Technology College of Materials Science and Engineering Zhejiang University of Technology Hangzhou 310014 China State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite Structures Guangxi Key Laboratory of Processing for Nonferrous Metals and Featured Materials MOE Key Laboratory of New Processing Technology for Nonferrous Metals and Materials School of Resources Environment and Materials Guangxi University Nanning 530004 China

The overuse and ineffective management of plastics have led to significant environmental pollution. Catalytic upcycling into value-added chemicals has emerged as a promising solution. This review provides a comprehensive overview of recent advances in catalytic upcycling, focusing on the cleavage of chemical bonds such as carbon-carbon (C-C), carbon-oxygen (C-O), and carbon-hydrogen (C-H) in plastics. It systematically discusses plastics conversion via electrocatalysis, thermal catalysis, and photocatalysis. Additionally, it explores the conversion of plastics into value-added chemicals and functional polymers. The review also addresses the challenges in this field and aims to offer insights for developing sustainable and effective plastics upcycling technologies.

关键词： plastics recycling catalysis chemical recycling value-added chemicals functional polymers

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Simplified liver imaging reporting and data system for the diagnosis of hepatocellular carcinoma on gadoxetic acid-enhanced magnetic resonance imaging

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2439-2448页

作者： Rong Lyu Wei-Juan Hu Di Wang Jiao Wang Yu-Bing Ye Ke-Feng Jia Department of Radiology Tianjin Third Central HospitalTianjin Key Laboratory of Extracorporeal Life Support for Critical DiseasesArtificial Cell Engineering Technology Research CenterTianjin 300170China

BACKGROUND The liver imaging reporting and data system(LI-RADS)diagnostic table has 15 cells and is too *** diagnostic performance of LI-RADS for hepatocellular carcinoma(HCC)is not satisfactory on gadoxetic acid-enhanced magnetic resonance imaging(EOB-MRI).AIM To evaluate the ability of the simplified LI-RADS(sLI-RADS)to diagnose HCC on *** A total of 331 patients with 356 hepatic observations were retrospectively *** diagnostic performance of sLI-RADS A-D using a single threshold was evaluated and compared with LI-RADS v2018 to determine the optimal *** algorithms of sLI-RADS A-D are as follows:The single threshold for sLI-RADS A and B was 10 mm,that is,classified observations≥10mm using an algorithm of 10-19 mm observations(sLI-RADS A)and≥20 mm observations(sLI-RADS B)in the diagnosis table of LI-RADS v2018,respectively,while the classification algorithm remained unchanged for observations<10 mm;the single threshold for sLI-RADS C and D was 20 mm,that is,for<20 mm observations,the algorithms for<10 mm observations(sLI-RADS C)and 10-19 mm observations(sLI-RADS D)were used,respectively,while the algorithm remained unchanged for observations≥20 *** hepatobiliary phase(HBP)hypointensity as a major feature(MF),the final sLI-RADS(F-sLI-RADS)was formed according to the optimal sLI-RADS,and its diagnostic performance was *** times needed to classify the observations according to F-sLIRADS and LI-RADS v2018 were *** The optimal sLI-RADS was sLI-RADS D(with a single threshold of 20 mm),because its sensitivity was greater than that of LI-RADS v2018(89.8%vs 87.0%,P=0.031),and its specificity was not lower(89.4%vs 90.1%,P>0.999).With HBP hypointensity as an MF,the sensitivity of F-sLI-RADS was greater than that of LI-RADS v2018(93.0%vs 87.0%,P<0.001)and sLI-RADS D(93.0%vs 89.8%,P=0.016),without a lower specificity(86.5%vs 90.1%,P=0.062;86.5%vs 89.4%,P=0.125).Compared with that of LI-RADS v2018,the time to classif

关键词： Hepatocellular carcinoma Magnetic resonance imaging Liver Diagnosis Contrast agent

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Preparation of Hydrophobic Fly Ash by Surface Modification and Oil-water Separation Devices

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Journal of Wuhan University of technology(Materials science) 2023年第3期38卷 695-700页

作者：于阳剑刘鹏 WANG Fazhou HU Chuanlin School of Chemistry Chemical Engineering and Life ScienceWuhan University of TechnologyWuhan 430070China State Key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China

A simple and effective superhydrophobic mesh was designed and made to separate oil-water mixture. Alkali-activated fly ash reacted with 1-bromooctadecane to prepare superhydrophobic modified fly ash (MFA) with low surface energy through Williamson ether synthesis. The MFA powder was then coated uniformly on a stainless steel mesh (SSM) along with the epoxy resin E44 and curing agent T31 to give the superhydrophobic MFA-modified stainless steel mesh (MFA-SSM). The MFA-SSM has a high static water contact angle (CA) of 150.1°and can separate various oil or organic solvent from water with>95%separation efficiency. The oil-water separation efficiency remained high after 30 runs of petroleum ether/water *** developed superhydrophobic stainless steel mesh is expected to have wider use in oil-water separation.

关键词： oil-water separation superhydrophobic stainless steel mesh Williamson ether synthesis

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