Metamaterials can control and manipulate acoustic/elastic waves on a subwavelength scale using cavities or additional ***,the large cavity and weak stiffness components of traditional metamaterials may cause a conflic...
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Metamaterials can control and manipulate acoustic/elastic waves on a subwavelength scale using cavities or additional ***,the large cavity and weak stiffness components of traditional metamaterials may cause a conflict between vibroacoustic reduction and load-bearing capacity,and thus limit their ***,we propose a lightweight multifunctional metamaterial that can simultaneously achieve low-frequency sound insulation,broadband vibration reduction,and excellent load-bearing performance,named as vibroacoustic isolation and bearing metamaterial(VIBM).The advent of additive manufacturing technology provides a convenient and reliable method for the fabrication of VIBM *** results show that the compressive strength of the VIBM is as high as 9.71 MPa,which is nearly 87.81%higher than that of the conventional grid structure(CGS)under the same volume ***,the vibration and sound transmission are significantly reduced over a low and wide frequency range,which agrees well with the experimental data,and the reduction degree is obviously larger than that obtained by the *** design strategy can effectively realize the key components of metamaterials and improve their application scenarios.
With continuous growth in scale,topology complexity,mission phases,and mission diversity,challenges have been placed for efficient capability evaluation of modern combat *** at the problems of insufficient mission con...
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With continuous growth in scale,topology complexity,mission phases,and mission diversity,challenges have been placed for efficient capability evaluation of modern combat *** at the problems of insufficient mission consideration and single evaluation dimension in the existing evaluation approaches,this study proposes a mission-oriented capability evaluation method for combat systems based on operation ***,a combat network model is given that takes into account the capability properties of combat ***,based on the transition matrix between combat nodes,an efficient algorithm for operation loop identification is proposed based on the Breadth-First *** the mission-capability satisfaction of nodes,the effectiveness evaluation indexes for operation loops and combat network are proposed,followed by node importance *** a case study of the combat scenario involving space-based support against surface ships under different strategies,the effectiveness of the proposed method is *** results indicated that the ROI-priority attack method has a notable impact on reducing the overall efficiency of the network,whereas the O-L betweenness-priority attack is more effective in obstructing the successful execution of enemy attack missions.
The rocket ejection seat is an advanced life-saving device for fighter pilots. However, it can also produce damaging impulse noise during ejection, which could compromise the pilot's hearing health, and the manufa...
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During ejection rescue, the parachute gun attached to a rocket-assisted ejection seat generates jet noise that may damage a pilot’s sensitive auditory organs;hence the effectiveness of hearing protectors, primarily a...
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Fatigue significantly impacts modern flight safety, leading to decreased pilot performance, judgment errors, flight illusions, and potentially severe accidents. Rocket ejection seat for emergency escape with additiona...
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In a recent study published in science,Zhang et *** that tripartite motif-containing(TRIM)protein 11(TRIM11)exhibited strong inhibition on tau aggregation,which is a pathological characteristic of tauopathies includin...
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In a recent study published in science,Zhang et *** that tripartite motif-containing(TRIM)protein 11(TRIM11)exhibited strong inhibition on tau aggregation,which is a pathological characteristic of tauopathies including Alzheimer’s disease(AD).1 This makes TRIM11 a promising novel target for the treatment of AD and other tauopathies.
Toxic arsenic(As)and trace element selenium(Se)are transformed by microorganisms but their complex interactions in soil-plant systems have not been fully *** Asand Se-oxidizing bacterium,Agrobacterium sp.T3F4,was appl...
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Toxic arsenic(As)and trace element selenium(Se)are transformed by microorganisms but their complex interactions in soil-plant systems have not been fully *** Asand Se-oxidizing bacterium,Agrobacterium sp.T3F4,was applied to a native seleniferous As-polluted soil to investigate As/Se uptake by the vegetable Brassica rapa *** As-Se interaction as mediated by strain *** Se content in the aboveground plants was significantly enhanced by 34.1%,but the As content was significantly decreased by 20.5% in the T3F4-inoculated pot culture compared to the control(P<0.05).Similar result was shown in treatment with additional 5 mg/kg of Se(IV)in *** addition,the As contents in roots were significantly decreased by more than 35% under T3F4 or Se(IV)treatments(P<0.05).Analysis of As-Se-bacterium interaction in a soil simulation experiment showed that the bioavailability of Se significantly increased and As was immobilized with the addition of the T3F4strain(P<0.05).Furthermore,an As/Se co-exposure hydroponic experiment demonstrated that As uptake and accumulation in plants was reduced by increasing Se(IV)*** 50% growth inhibition concentration(IC50)values for As in plants were increased about one-fold and two-fold under co-exposure with 5 and 10μmol/L Se(IV),*** conclusion,strain T3F4 improves Se uptake but decreases As uptake by plants via oxidation of As and Se,resulting in decrease of soil As bioavailability and As/Se competitive absorption by *** provides a potential bioremediation strategy for Se biofortification and As immobilization in As-polluted soil.
During the ejection process of the fighter pilot, the parachute is shot out by the life-saving seat gun, which generates a shockwave that endangers human safety. In order to accurately and stably extract the time-freq...
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Congenital disorders of glycosylation(CDG)are a cluster of monogenic disorders resulting from defects in *** encodes fucokinase,an enzyme that catalyzes the phosphorylation of L-fucose to generate fucose-1-phosphate,a...
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Congenital disorders of glycosylation(CDG)are a cluster of monogenic disorders resulting from defects in *** encodes fucokinase,an enzyme that catalyzes the phosphorylation of L-fucose to generate fucose-1-phosphate,an important step in *** in FCSK lead to CDG with an autosomal recessive inheritance pattern,primarily manifesting as developmental delay,hypotonia,and brain ***,no fcsk mutant animal models have yet been *** study constructed the first fcsk knockout(fcsk^(-/-))zebrafish model using CRISPR/Cas9 ***,fcsk^(-/-)zebrafish exhibited impaired growth,characterized by delayed epiboly and DNA accumulation during early embryonic development,as well as brain atrophy in ***-stage fcsk^(-/-)zebrafish displayed locomotor deficits and increased susceptibility to pentylenetetrazole-induced *** adulthood,fcsk^(-/-)zebrafish showed neurodevelopmental abnormalities,including increased anxiety,decreased aggression,reduced social preference,and impaired ***,total protein fucosylation was markedly reduced in fcsk^(-/-)zebrafish,accompanied by decreased expression of pofut2,which encodes protein Ofucosyltransferase 2,an enzyme involved in the fucosylation salvage *** activity was elevated in the midbrain-hindbrain boundary(MHB)of fcsk^(-/-)*** with GDP-L-fucose or the human FCSK gene restored developmental defects and total protein fucosylation in fcsk^(-/-)*** sequencing revealed dysregulated gene expression associated with glycosylation,apoptosis,and neurodegenerative *** findings suggest that fcsk^(-/-)zebrafish exhibit neurodevelopmental disorders,providing the first fcsk gene knockout animal model and offering a platform for investigating the molecular underpinnings of the disease and facilitating drug screening efforts.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNAER) family proteins are the engines of most intra-cellular and exocytotic membrane fusion pathways(Jahn and Scheller 2006).Over the past two dec...
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Soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNAER) family proteins are the engines of most intra-cellular and exocytotic membrane fusion pathways(Jahn and Scheller 2006).Over the past two decades, in-vitro liposome fusion has been proven to be a powerful tool to reconstruct physiological SNARE-mediated membrane fusion processes(Liu et al. 2017). The reconstitution of the membrane fusion process not only provides direct evidence of the capability of the cognate SNARE complex in driving membrane fusion but also allows researchers to study the functional mechanisms of regulatory proteins in related pathways(Wickner and Rizo 2017). Heretofore, a variety of delicate methods for in-vitro SNARE-mediated liposome fusion have been established(Bao et al. 2018;Diao et al. 2012; Duzgunes 2003; Gong et al. 2015; Heo et al. 2021; Kiessling et al. 2015; Kreye et al.2008; Kyoung et al. 2013; Liu et al. 2017; Scott et al. 2003). Although technological advances have made reconstitution more physiologically relevant, increasingly elaborate experimental procedures, instruments, and data processing algorithms nevertheless hinder the non-experts from setting up basic SNARE-mediated liposome fusion assays. Here, we describe a low-cost, timesaving, and easy-to-handle protocol to set up a foundational in-vitro SNARE-mediated liposome fusion assay based on our previous publications(Liu et al. 2023; Wang and Ma 2022). The protocol can be readily adapted to assess various types of SNARE-mediated membrane fusion and the actions of fusion regulators by using appropriate alternative additives(e.g., proteins, macromolecules, chemicals, etc.). The total time required for one round of the assay is typically two days and could be extremely compressed into one day.
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