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The high quality genome of potherb mustard Xuecai(Brassica juncea ***)provides new insights into leaf shape variation

Journal of Integrative Agriculture 2025年第4期24卷 1461-1476页

作者： Shuangping Heng Mengdi Cui Xiaolin Li Shaoheng Zhang Guangzhi Mao Feng Xing Zhengjie Wan Jing Wen Jinxiong Shen Tingdong Fu College of Life Sciences Institute for Conservation and Utilization of Agro-Bioresources in Dabie MountainsXinyang Normal UniversityXinyang 464000China College of Horticulture and Forestry National Key Laboratory for Germplasm Innovation&Utilization of Horticultural CropsHuazhong Agricultural UniversityWuhan 430070China College of Plant Science and Technology National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhan 430070China

The potherb mustard Xuecai(XC)cultivar is a cruciferous vegetable that is popular either fresh or *** to the deep notches in the edges of leaves in mustard XC,this plant can be said to have multipinnately lobed *** net photosynthesis of lobed leaves is significantly greater than that of simple ***,the molecular mechanism of leaf shape variation has not been ***,we used HiFi and Hi-C data to assemble the XC *** genome was 961.72 Mb in size,with a contig N50 value of 6.565 *** XC genome was compared with four previously sequenced mustard genomes,and the genomic collinearity regions,SNPs,and indels were *** BjRCO genes were found on chromosome(Chr.)A10 in potherb mustard XC when the BjRCO gene locus was compared against other sequenced *** *** duplication was found to contribute to the BjRCO gene copy *** transcript expression of BjRCO genes was greater in multipinnately lobed leaves than in sawtooth-like ***,these findings indicate that both the greater copy number and the expression level of BjRCO genes regulate leaf shape from simple to complex in *** editing of the BjRCO gene from XC changed the leaf shape from multipinnately lobed to *** high-quality XC genome sequence not only provides new insight into *** leaf-type genomics but also helps in deciphering leaf shape *** study provides insights into the variation and evolution of important traits in Brassica plants through a comparative analysis of the sequenced genomes.

关键词： Brassica juncea genome sequence multipinnately lobed leaves BjRCO gene editing

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Photo-controllable multiple and orthogonal regulation of gene expression by chemically modified oligonucleotides

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science China Chemistry 2025年第2期68卷 694-704页

作者： Yang Wang Qiu-Long Zhang Yan Liu Liang-Liang Wang Chao-Qun Wu Ming Shao Xiwen Xing Juan Du Liang Xu Key Laboratory of Tropical Biological Resources of Ministry of Education and One Health Institute School of Pharmaceutical SciencesHainan UniversityHaikou 570228China Central Laboratory Department of The Second Affiliated Hospital School of MedicineThe Chinese University of Hong KongShenzhen&Longgang District People’s Hospital of ShenzhenShenzhen 518172China MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275China Department of Biotechnology College of Life Science and TechnologyJinan University InstitutionGuangzhou 510632China School of Pharmacy and Medical Technology Putian UniversityPutian 351100China

Gene expression must be precisely regulated in cells and functional nucleic acids are the most widely utilized tools for gene *** of how these nucleic acid tools work in the cellular environment can precisely manipulate gene expression through a non-invasive *** we report a methodology on multiplex photocontrol of functional nucleic acids to achieve totally temporal and orthogonal regulation of gene expression in living *** select two functional nucleic acid systems as examples,DNAzyme and CRISPR/Cas9,and demonstrate the power of light control for precise gene manipulation by rational design of chemically modified oligonucleotides through introduction of two photocleavable *** the previous modulation of functional nucleic acids by simply activating or deactivating,we successfully achieve versatile controlling patterns using light as the governing *** design represents a generalized pathway towards the photo-controllable functional nucleic acids,which greatly enriches the toolbox for optogenetic studies.

关键词： functional nucleic acids optogenetics modified oligonucleotides gene technology

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Both 20S and 19S proteasome components are essential for meiosis in male mice

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Zoological Research 2025年第1期46卷 27-40页

作者： Ting-Ting Han Li-Ying Wang Qiu-Xing Zhou Wei Wei Yan-Jie Ma Ying-Hong Chen Wei Li Zhen-Yu Ju Chao Liu Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Aging and Regenerative MedicineDepartment of Developmental&Regenerative MedicineCollege of Life Science and TechnologyJinan UniversityGuangzhouGuangdong 510632China Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhouGuangdong 510623China

The proteasome,an evolutionarily conserved proteolytic complex comprising the 20S core particle and 19S regulatory particles,performs both shared and distinct functions across various tissues and ***,a highly complex developmental process,relies on proteasome activity at multiple stages to regulate protein *** this study,we selected the 20S subunit PSMA1 and 19S regulatory subunit PSMD2 to investigate the potential functions of the proteasome in *** Psma1-EGFP and Psmd2-mCherry knock-in mouse models,we confirmed the expression of both subunits in all spermatogenic cell types,with pronounced presence in early germ cell *** further clarify their functional significance,we specifically knocked out Psma1 and Psmd2 in germ *** of either PSMA1 or PSMD2 led to disrupted spermatogenesis,characterized by the complete absence of sperm in the *** analysis indicated that loss of these proteasome components impaired meiotic ***1 and Psmd2 knockout germ cells showed accumulation of DMRT1,a key regulator of mitosis-to-meiosis transition,leading to a reduction in STRA8 levels and consequent disruption of meiosis *** study sheds light on the molecular mechanisms that govern meiotic initiation and identifies potential genes associated with male infertility.

关键词： Proteasome PSMA1 PSMD2 Meiotic initiation Spermatogenesis

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Evaluating the global thiols redox state in living cells using a reducing sulfur species responsive fiuorescence switching platform

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Chinese Chemical Letters 2025年第4期36卷 338-344页

作者： Hui Zhang Rong Feng Wanyi Yu Hongbei Wei Tianhong Wu Peng Zhang Wenhai Bian Xin Li Di Gao Guojun Weng Zhe Yang Tony D.James Xiaolong Sun The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi'an Jiaotong UniversityXi'an 710049China Department of Chemistry University of BathBath BA27AYUnited States School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

Redox dyshomeostasis is a critical factor in the initiation of numerous diseases,making the accurate evaluation of the redox status of the cellular environment an important aspect of physiological ***,maintaining redox homeostasis relies on a complex and dynamic physiological system involving multiple substrate-enzyme interactions,so its accurately detection remains a *** this research,we developed an activable fiuorescence switching platform by incorporating different conjugate acceptors to a fiuorophore using ester bonds and resulting in fiuorescence quenching due to donor-excited photo-induced electron transfer(d-Pe T),which was confirmed through density functional theory *** reaction-based probe was deployed for recognizing all major intracellular reducing sulfur species(RSS),including H_(2)S,cysteine(Cys),homocysteine(Hcy),glutathione(GSH),and protein free *** quenched fiuorescence was significantly recovered by RSS,through releasing the fiuorophore and diminishing the d-Pe T ***,the fiuorescent probe was used for the sensing and imaging RSS in living cells,demonstrating good cell-permeability,low cytotoxicity,and negative correlation with reactive oxygen species content,enabling the evaluating of global thiols redox state in Hep G2 cellular lines during ferroptosis processes.

关键词： Fluorescent probe Reducing sulfur species Donor-excited photo-induced electron transfer Thiols redox state Ferroptosis

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CDK1-mediated phosphorylation of USP37 regulates SND1 stability and promotes oncogenesis in colorectal cancer

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Acta Pharmaceutica Sinica B 2025年第4期15卷 1938-1955页

作者： Wu, Liang Cheng, Can Zhao, Ning Zhu, Liang Li, Heng Liu, Jingwen Wu, Yang Chen, Xi Yao, Hanhui Liu, Lianxin Department of Gastrointestinal Surgery the First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery Hefei 230001 China Department of Vascular Surgery the First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery Hefei 230001 China Department of Surgical Oncology the First Affiliated Hospital of Xi'an JiaoTong University Xi'an 710061 China Department of Comprehensive Surgery Anhui Provincial Cancer Hospital West District of the First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230001 China Health Management Center the First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China Heifei 230001 China Department of Hepatobiliary Surgery the First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery Anhui Provincial Clinical Research Center for Hepatobiliary Diseases Hefei 230001 China

Colorectal cancer (CRC) poses a severe global health challenge with high incidence and mortality rates. USP37 has been identified as the bona fide deubiquitinase of SND1, playing a critical role in stabilizing SND1, thereby augmenting its oncogenic potential. The interaction between USP37 and SND1 was confirmed through extensive proteomics, ubiquitinomics, and interactomics, underscoring their synergistic effects on CRC proliferation and metastasis. Additionally, CDK1 has emerged as a pivotal regulator of USP37, phosphorylating it at threonine 631 rather than serine 628, enhancing its deubiquitinase activity, and consequently stabilizing SND1 to drive CRC malignancy further. Histological analyses of human CRC samples linked the upregulation of CDK1 and USP37 with increased SND1 levels and poor patient prognosis. High-throughput virtual screening and subsequent experimental validation identified Dacarbazine as a pharmacological inhibitor of USP37, and its inhibition disrupted SND1 stability, hindering CRC cell proliferation and metastasis. This study reveals a novel and promising molecular mechanism driving CRC progression through the CDK1–USP37–SND1 axis, highlighting the clinical importance of targeting this pathway to improve patient outcomes. © 2025 The Authors

关键词： CDK1 Colorectal cancer Dacarbazine Deubiquitination Oncogenesis Phosphorylation SND1 USP37

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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai Chen Dan Liu Qian Xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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The cryptic lncRNA-encoded microprotein TPM3P9 drives oncogenic RNA splicing and tumorigenesis

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Signal Transduction and Targeted Therapy 2025年第2期10卷 1069-1085页

作者： Kun Meng Yuying Li Xiaoyi Yuan Hui-Min Shen Li-Ling Hu Danya Liu Fujin Shi Dandan Zheng Xinyu Shi Nengqiao Wen Yun Cao Yun-Long Pan Qing-Yu He Chris Zhiyi Zhang MOE Key Laboratory of Tumor Molecular Biology and State Key Laboratory of Bioactive Molecules and Druggability Assessment Institute of Life and Health EngineeringCollege of Life Science and TechnologyJinan UniversityGuangzhou 510632China Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and ScienceHubei Province 441100 XiangyangChina Department of Obstetrics and Gynecology The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Department of Pathology State Key Laboratory of Oncology in South ChinaSun Yat-sen University Cancer Center510060 GuangzhouChina The First Affiliated Hospital of Jinan University Jinan UniversityGuangzhou 510632China

Emerging evidence demonstrates that cryptic translation from RNAs previously annotated as noncoding might generate microproteins with oncogenic ***,the importance and underlying mechanisms of these microproteins in alternative splicing-driven tumor progression have rarely been ***,we show that the novel protein TPM3P9,encoded by the lncRNA tropomyosin 3 pseudogene 9,exhibits oncogenic activity in clear cell renal cell carcinoma(ccRCC)by enhancing oncogenic RNA *** of TPM3P9 promotes cell proliferation and tumor ***,TPM3P9 binds to the RRM1 domain of the splicing factor RBM4 to inhibit RBM4-mediated exon skipping in the transcription factor *** results in increased expression of the oncogenic splice variant TCF7L2-L,which activates NF-κB signaling via its interaction with SAM68 to transcriptionally induce RELB *** a clinical perspective,TPM3P9 expression is upregulated in cancer tissues and is significantly correlated with the expression of TCF7L2-L and *** TPM3P9 expression or low RBM4 expression is associated with poor survival in patients with ***,our findings functionally and clinically characterize the“noncoding RNA”-derived microprotein TPM3P9 and thus identify potential prognostic and therapeutic factors in renal cancer.

关键词： tumorigenesis enhancing oncogenic rna splicingoverexpression lncrna tropomyosin cryptic lncrna encoded microprotein clear cell renal cell carcinoma ccrcc oncogenic rna splicing cryptic translation alternative splicing

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Genome-wide identification and expression analysis of BvHDA genes in sugar beet (Beta vulgaris L.) under salt and drought stress

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Plant Growth Regulation 2025年 1-14页

作者： Wei, Ming Zhao, Meng Nan, Xin-Xin Wu, Guo-Qiang Chen, Xiang-Yu Zhuang, Yong School of Life Science and Engineering Lanzhou University of Technology No.36 Pengjiaping Road Lanzhou 730050 China Biomass Energy Technology Research Centre Key Laboratory of Development and Application of Rural Renewable Energy Biogas Institute of Ministry of Agriculture and Rural Affairs Chengdu 610041 China

Zinc ion (Zn2⁺)-dependent histone deacetylases (HDAs) are key epigenetic regulators of chromatin remodeling and serve crucial functions in plant stress adaptation. Although HDAs have been extensively studied in variou... 详细信息

Zinc ion (Zn²⁺)-dependent histone deacetylases (HDAs) are key epigenetic regulators of chromatin remodeling and serve crucial functions in plant stress adaptation. Although HDAs have been extensively studied in various species, their functional characterization and roles in abiotic stress responses in sugar beet (Beta vulgaris L.), an essential halophilic crop for food and sugar production, remain largely unclear. In this study, eight BvHDA genes in sugar beet were identified through a comprehensive bioinformatics analysis. The coding sequences of BvHDAs ranged from 984 to 1926 bp, with exon numbers varying between 3 and 14, which reflects their diverse structural organization. Based on phylogenetic analysis, BvHDAs can be classified into three distinct groups: Class I (three members), II (four members), and III (one member). Subcellular localization predictions suggested that BvHDAs were predominantly in the nucleus, which is consistent with their role in histone deacetylation. Expression profiling revealed that BvHDAs were responsive to salt and drought stress in roots and shoots, indicating their involvement in abiotic stress adaptation. To further explore the roles of BvHDAs, we overexpressed BvHDA4 in Arabidopsis, resulting in increased salt sensitivity, as characterized by dwarfism, etiolation, shortened roots, and reduced fresh weight. These findings indicate that BvHDA4 negatively regulates salt tolerance in plants. In summary, this study provides a systematic analysis of BvHDAs and highlights their importance in abiotic stress, establishing a research framework and valuable genetic resources for enhancing stress tolerance in sugar beet and other crops. © The Author(s) 2025.

关键词： Abiotic stress Bioinformatic analysis Gene expression Histone deacetylase Salt tolerance

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Tailoring solvation sheath for rechargeable zinc-ion batteries:Progress and prospect

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Materials Reports(Energy) 2025年第1期5卷 3-18页

作者： Xiaomin Cheng Jing Dong Haifeng Yang Xiang Li Xinyu Zhao Bixian Chen Yongzheng Zhang Meinan Liu Jian Wang Hongzhen Lin i-Lab&CAS Key Laboratory of Nanophotonic Materials and Devices Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou215123China State Key Laboratory of Chemical Engineering East China University of Science and TechnologyShanghai200237China Anhui Provincial Key Laboratory of Green Carbon Chemistry Fuyang Normal UniversityFuyang236037China State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite Structures Guangxi Key Laboratory of Processing for Non-Ferrous Metals and Featured MaterialsSchool of ResourcesEnvironment and MaterialsGuangxi UniversityNanning530004China Helmholtz Institute Ulm(HIU) D89081 UlmKarlsruhe Institute of Technology(KIT)D76021 KarlsruheGermany School of Materials Science and Engineering Jiangsu UniversityZhenjiang212013JiangsuChina

Aqueous zinc-metal based batteries(AZMBs)perfectly combine safety,economy and pro-environment,but their performance is arresting limited by the interfacial instability caused by the large desolvation energy barrier of[Zn(H2O)6]^(2+)and the massive release of active water at the electrolyte/electrode *** this review,we briefly outline the solvation structure of zinc ions and the necessity of ***,the variety of strategies to solve these issues,mainly including reorganizing solvation sheath by changing electrolyte environment and accelerating interface desolvation by constructing artificial interfacial layer,are categorically discussed and systematically ***,perspectives and suggestions regarding desolvation theories,interfacial evolution,material design and analysis techniques are proposed to design highly stable zinc anodes.

关键词： Aqueous zinc-metal based batteries Desolvation Electrolyte strategy Interfacial layer

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Peri-centrosomal localization of small interfering RNAs in ***

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science China(life sciences) 2025年第4期68卷 895-911页

作者： Qile Jin Xuezhu Feng Minjie Hong Ke Wang Xiangyang Chen Jiewei Cheng Yan Kuang Xiaoyue Si Mingjing Xu Xinya Huang Shouhong Guang Chengming Zhu Department of Obstetrics and Gynecology The First Affiliated Hospital of USTCThe USTC RNA InstituteMinistry of Education Key Laboratory for Membraneless Organelles&Cellular DynamicsHefei National Research Center for Physical Sciences at the MicroscaleCenter for Advanced Interdisciplinary Science and Biomedicine of IHMSchool of Life SciencesDivision of Life Sciences and MedicineBiomedical Sciences and Health Laboratory of Anhui ProvinceUniversity of Science and Technology of ChinaHefei 230027China School of Basic Medicine Anhui Medical UniversityHefei 230032China

The centrosome is the microtubule-organizing center and a crucial part of cell *** RNAs(cnRNAs)have been reported to enable precise spatiotemporal control of gene expression during cell division in many *** and how cnRNAs exist in *** are ***,using the nuclear RNAi Argonaute protein NRDE-3 as a reporter,we observed potential peri-centrosome localized small interfering(si)RNAs in ***-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary *** generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2,other centriole proteins and pericentriolar material(PCM)components in *** *** peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3,which is essential for siRNA *** of eri-1,ergo-1,or drh-3 significantly increased the percentage of pericentrosome-enriched *** the metaphase of the cell cycle,NRDE-3 was enriched in both the peri-centrosomal region and the ***,the integrity of centriole proteins and pericentriolar material(PCM)components is also required for the peri-centrosomal accumulation of ***,we concluded that siRNAs could accumulate in the pericentrosomal region in *** and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

关键词： SiRNA centrosomal RNAs NRDE-3

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