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GhAP1-D3 positively regulates flowering time and early maturity with no yield and fiber quality penalties in upland cotton

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Journal of Integrative Plant Biology 2023年第4期65卷 985-1002页

作者： Caixiang Wang Juanjuan Liu Xiaoyu Xie Ji Wang Qi Ma Pengyun Chen Delong Yang Xiongfeng Ma Fushun Hao Junji Su State Key Laboratory of Aridland Crop Science College of Life Science and TechnologyGansu Agricultural UniversityLanzhou 730070China State Key Laboratory of Cotton Biology College of Life ScienceHenan UniversityKaifeng 475004China Cotton Research Institute Xinjiang Academy of Agricultural and Reclamation ScienceShihezi 832000China State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000China

Flowering time(FTi)is a major factor determining how quickly cotton plants reach *** maturity greatly affects lint yield and fiber quality and is crucial for mechanical harvesting of cotton in northwestern ***,few quantitative trait loci(QTLs)or genes regulating early maturity have been reported in cotton,and the underlying regulatory mechanisms are largely *** this study,we characterized 152,68,and 101 loci that were significantly associated with the three key early maturity traits—FTi,flower and boll period(FBP)and whole growth period(WGP),respectively,via four genome-wide association study methods in upland cotton(Gossypium hirsutum).We focused on one major early maturity-related genomic region containing three single nucleotide polymorphisms on chromosome D03,and determined that GhAP1-D3,a gene homologous to Arabidopsis thaliana APETALA1(AP1),is the causal locus in this *** plants overexpressing GhAP1-D3 showed significantly early flowering and early maturity without penalties for yield and fiber quality compared to wild-type(WT)*** contrast,the mutant lines of GhAP1-D3 generated by genome editing displayed markedly later flowering than the ***1-D3 interacted with GhSOC1(SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1),a pivotal regulator of FTi,both in vitro and in *** in GhAP1-D3 transcript levels clearly affected the expression of multiple key flowering regulatory ***,DNA hypomethylation and high levels of H3K9ac affected strong expression of GhAP1-D3 in early-maturing cotton *** propose that epigenetic modifications modulate GhAP1-D3 expression to positively regulate FTi in cotton through interaction of the encoded GhAP1 with GhSOC1 and affecting the transcription of multiple flowering-related *** findings may also lay a foundation for breeding early-maturing cotton varieties in the future.

关键词： upland cotton early maturity genome‐wide association studies QTL AP1 flowering time

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Ratiometric fluorescent Si-FITC nanoprobe for immunoassay of SARS-CoV-2 nucleocapsid protein

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Nano Research 2023年第2期16卷 2859-2865页

作者： Guobin Mao Silu Ye Wen Yin Yang Yang Xinghu Ji Jin He Yingxia Liu Junbiao Dai Zhike He Yingxin Ma CAS Key Laboratory of Quantitative Engineering Biology Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China State Key Laboratory of Agricultural Microbiology College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People's HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China

Coronavirus disease 2019(COVID-19)highlights the importance of rapid and reliable diagnostic assays for the management of virus ***,we developed a one-pot hydrothermal method to prepare Si-FITC nanoparticles(NPs)for the fluorescent immunoassay of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleocapsid protein(N protein).The synthesis of Si-FITC NPs did not need post-modification,which addressed the issue of quantum yield reduction during the coupling ***-FITC NPs showed two distinct peaks,Si fluorescence atλem=385 nm and FITC fluorescence atλem=490 *** the presence of KMnO_(4),Si fluorescence was decreased and FITC fluorescence was ***,in the presence of N protein,catalase(CAT)-linked secondary antibody/reporter antibody/N protein/capture antibody immunocomplexes were formed on ***,hydrogen peroxide(H_(2)O_(2))and Si-FITC NPs/KMnO_(4)were injected into the microplate *** decomposition of H_(2)O_(2)by CAT resulted in remaining of KMnO_(4),which changed the fluorescence intensity ratio of Si-FITC *** fluorescence intensity ratio correlated significantly with the N protein concentration ranging from 0.02 to 50.00 ng/mL,and the detection limit was 0.003 ng/mL,which was more sensitive than the commercial ELISA kit with a detection limit of 0.057 ng/*** N protein concentration can be accurately determined in human ***,the COVID-19 and non-COVID-19 patients were distinguishable by this ***,the ratiometric fluorescent immunoassay can be used for SARS-CoV-2 infection diagnosis with a high sensitivity and selectivity.

关键词： Si-FITC nanoparticles ratiometric fluorescent probe SARS-CoV-2 ELISA

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SlTrxh functions downstream of SlMYB86 and positively regulates nitrate stress tolerance via S-nitrosation in tomato seedling

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园艺研究（英文） 2024年第9期11卷 27-39页

作者： Senlin Zeng Xudong Sun Jiali Zhai Xixian Li García-Caparrós Pedro Hongjuan Nian Kunzhi Li Huini Xu Faculty of Life Science and Technology Kunming University of Science and TechnologyJingming South StreetKunmingYunnan 650224China Yunnan Key Laboratory of Crop Wild Relatives The Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China Department of Agronomy University of Almeria04120AlmeriaSpain

Nitric oxide(NO)is a redox-dependent signaling molecule that plays a crucial role in regulating a wide range of biological processes in *** functions by post-translationally modifying proteins,primarily through ***(Trx),a small and ubiquitous protein with multifunctional properties,plays a pivotal role in the antioxidant defense ***,the regulatory mechanism governing the response of tomato Trxh(SlTrxh)to excessive nitrate stress remains *** this study,overexpression or silencing of SlTrxh in tomato led to increased or decreased nitrate stress tolerance,*** overexpression of SlTrxh resulted in a reduction in levels of reactive oxygen species(ROS)and an increase in S-nitrosothiol(SNO)contents;conversely,silencing SlTrxh exhibited the opposite *** level of S-nitrosated SlTrxh was increased and decreased in SlTrxh overexpression and RNAi plants after nitrate treatment,*** was found to be susceptible to S-nitrosation both in vivo and in vitro,with Cysteine 54 potentially being the key site for *** interaction assays revealed that SlTrxh physically interacts with SlGrx9,and this interaction is strengthened by ***,a combination of yeast one-hybrid(Y1H),electrophoretic mobility shift assay(EMSA),chromatin immunoprecipitation-quantitative PCR(ChIP-qPCR),and transient expression assays confirmed the direct binding of SlMYB86 to the SlTrxh promoter,thereby enhancing its ***86 is located in the nucleus and SlMYB86 overexpressed and knockout tomato lines showed enhanced and decreased nitrate stress tolerance,*** findings indicate that SlTrxh functions downstream of SlMYB86 and highlight the potential significance of S-nitrosation of SlTrxh in modulating its function under nitrate stress.

关键词： tolerance stress tomato downstream functions nitrate positively regulates s-nitrosation seedling

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Association between MTHFR c.677C>T variant and erectile dysfunction among males attending fertility clinic

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Asian Journal of Andrology 2024年第1期26卷 41-45页

作者： Shun Bai Ming-Zhen Li Yang-Yang Wan Xue-Chun Hu Yi-Xun Liu Xian-Hong Tong Tong-Hang Guo Lu Zong Ran Liu Yuan-Qi Zhao Ping Xiang Bo Xu Xiao-Hua Jiang Department of Gynecology and Obstetrics Reproductive and Genetic HospitalThe First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China NHC Key Laboratory of Male Reproduction and Genetics Guangdong Provincial Reproductive Science Institute(Guangdong Provincial Fertility Hospital)Guangzhou 510600China Department of Urology The First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Wannan Medical College Wuhu 241002China

Genetic risk factors have been shown to contribute to the development of sexual ***,the role of methylenetetrahydrofolate reductase(MTHFR)gene variants in the risk of erectile dysfunction(ED)remains *** this study,we recruited 1254 participants who underwent ED assessed by the International Index of Erectile *** MTHFR c.677C>T variant was also measured by fluorescence polymerase chain reaction(PCR).No significant difference in the genotypic frequency of the MTHFR C677T polymorphism(CC,CT,and TT)was observed between men from the ED and non-ED *** addition,on binary logistic regression analysis,both crude and adjusted models showed that the risk of ED was not significantly associated with the C677T ***,a significantly higher frequency of the 677TT polymorphism was found in severe and moderate ED(P=O.02).The positive correlation between the MTHFR 677TT polymorphism and severe ED was confirmed by logistic regression analysis,even after adjusting for potential confounders(odds ratio[OR]=2.46,95%confidence interval[CI]:1.15-5.50,P=0.02).These findings suggest a positive correlation between the MTHFR 677TT polymorphism and the risk of severe *** of MTHFR gene polymorphisms may provide complementary information for ED patients during routineclinicaldiagnosis.

关键词： erectile dysfunction gene variant genetic risk factors methylenetetrahydrofolate reductase sexual function

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High-gain signal-on PEDOT:PSS organic photoelectrochemical transistor biosensing modulated by a MXene/MOFs/NiO Schottky heterojunction

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science China Chemistry 2023年第2期66卷 578-585页

作者： Zheng Li Yi-Tong Xu Jin Hu Ting Wang Fang-Qing Liu Hong Zhou Guang-Xu Chen Peng Lin Wei-Wei Zhao Jing-Juan Xu Hong-Yuan Chen State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China Shenzhen Key Laboratory of Special Functional Materials&Guangdong Research Center for Interfacial Engineering of Functional Materials College of Materials Science and EngineeringShenzhen UniversityShenzhen518060China Shandong Key Laboratory of Biochemical Analysis College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao266042China School of Environment and Energy State Key Laboratory of Luminescent Materials and DevicesGuangdong Provincial Key Laboratory of Atmospheric Environment and Pollution ControlSouth China University of TechnologyGuangzhou510006China

Herein we report a high-gain signal-on poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate)(PEDOT:PSS)organic electrochemical transistor(OECT)biosensing using an accumulation-mode PEDOT:PSS OECT modulated by a light-fueled MXene/MOFs/Ni O Schottky *** such a system,the MXene/MOFs/Ni O Schottky heterojunction exhibited superior gating effect,as it not only enabled the fast-directional charge transfer but also guaranteed the maximal accessibility of the electrolyte to topped 2D MXene with large surface *** linkage with a bi-enzyme cascade system,the quinone derivatives produced by the cascade reaction of alkaline phosphatase(ALP)and tyrosinase(TYR)could serve as effective electron acceptors for the representative Ti_(3)C_(2)/PCN-224/Ni O heterojunction,underpinning an innovative method for sensitive detection of ALP activity with a low detection limit of 0.001 U L^(-1).Remarkably,the as-developed system demonstrated a remarkable current gain as high as near 10^(4),which to our knowledge is the highest one among existing OECT biosensory *** work represents a generic protocol to develop the novel signal-on PEDOT:PSS OECT platform towards biochemical detection and beyond.

关键词： signal-on PEDOT:PSS organic electrochemical transistor biosensing Schottky heterojunction

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Plant Intron-Splicing Efficiency Database(PISE):exploring splicing of~1,650,000 introns in Arabidopsis,maize,rice,and soybean from~57,000 public RNA-seq libraries

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science China(life sciences) 2023年第3期66卷 602-611页

作者： Hong Zhang Jinbu Jia Jixian Zhai Department of Biology School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Institute of Plant and Food Science Southern University of Science and TechnologyShenzhen 518055China Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Institute of Plant and Food ScienceDepartment of BiologySouthern University of Science and TechnologyShenzhen 518055China

Intron retention is the most common alternative splicing event in plants and plays a crucial role in the responses of plants to environmental *** a large number of RNA-seq libraries from different treatments and genetic mutants stored in public domains,a resource for querying the intron-splicing ratio of individual intron is still ***,we established the first-ever large-scale splicing efficiency database in any *** database includes over 57,000 plant public RNA-seq libraries,comprising 25,283 from Arabidopsis,17,789 from maize,10,710 from rice,and 3,974 from soybean,and covers a total of 1.6 million introns in these four *** addition,we manually curated and annotated all the mutant-and treatment-related libraries as well as their matched controls included in our library collection,and added graphics to display intron-splicing efficiency across various tissues,developmental stages,and stress-related *** result is a large collection of 3,313treatment conditions and 3,594 genetic mutants for discovering differentially regulated splicing *** online database can be accessed at https://***/.

关键词： splicing RNA-seq plant database

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Consistent Alterations of Human Fecal Microbes After Transplantation into Germ-free Mice

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Genomics, Proteomics & Bioinformatics 2022年第2期20卷 382-393页

作者： Yanze Li Wenming Cao Na L Gao Xing-Ming Zhao Wei-Hua Chen MOE Key Laboratory of Molecular Biophysics Hubei Key Laboratory of Bioinformatics and Molecular-imagingDepartment of Bioinformatics and Systems BiologyCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Institute of Science and Technology for Brain-Inspired Intelligence(ISTBI) Fudan UniversityShanghai 200433China College of Life Science Henan Normal UniversityXinxiang 453007China

Fecal microbiota transplantation(FMT)of human fecal samples into germ-free(GF)mice is useful for establishing causal relationships between the gut microbiota and human ***,due to the intrinsic differences between human and mouse intestines and the different diets of the two organisms,it may not be possible to replicate human phenotypes in mice through FMT;similarly,treatments that are effective in mouse models may not be effective in *** this study,we aimed to identify human gut microbes that undergo significant and consistent changes(i.e.,in relative abundances)after transplantation into GF mice in multiple experimental *** collected 16S rDNA-seq data from four published studies and analyzed the gut microbiota profiles from 1713 human–mouse ***,on average,we found that only 47%of the human gut microbes could be re-established in mice at the species level,among which more than 1/3 underwent significant changes(referred to as“variable taxa”).Most of the human gut microbes that underwent significant changes were consistent across multiple human–mouse pairs and experimental ***,about 1/3 of human samples changed their enterotypes,i.e.,significant changes in their leading species after *** fed with a controlled diet showed a lower enterotype change rate(23.5%)than those fed with a noncontrolled diet(49.0%),suggesting a possible solution for *** of the variable taxa have been reported to be implicated in human diseases,with some recognized as the causative *** results highlight the challenges of using a mouse model to replicate human gut microbiota-associated phenotypes,provide useful information for researchers using mice in gut microbiota studies,and call for additional validations after *** online database named FMT-DB is publicly available at http://***/#/.

关键词： Germ-free mice Fecal microbiota transplantation Gut microbe Enterotype 16S rDNA

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The role of PilU in the surface behaviors of Pseudomonas aeruginosa

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mlife 2025年第1期4卷 83-95页

作者： Jingchao Zhang Yan Luo Yiwu Zong Shangping Lu Yi Shi Fan Jin Kun Zhao Center for Medical Genetics Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduChina Key Laboratory of SystemsBioengineering(Ministry of Education) School of Chemical Engineering and TechnologyFrontiers Science Center for Synthetic BiologyTianjin UniversityTianjinChina Guangzhou General Institute of Medical Research GuangzhouChina School of Life Science and Technology University of Electronic Science and Technology of ChinaChengduChina The Sichuan Provincial Key Laboratory for Human Disease Gene Study The Institute of Laboratory MedicineSichuan Provincial People's HospitalUniversityof Electronic Science and Technology of ChinaChengduChina CAS Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic BiologyShenzhenInstitutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina Institute of Fundamental and Frontier Sciences University of Electronic Science andTechnology of ChinaChengduChina

In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial *** PilU isconsidered a homolog of the TFP disassembling motor PilT,its speciﬁc roles remain *** pilus visualization andsingle-cell tracking techniques,we characterized TFP dynamics and surface behaviors in wild-type andΔpilU *** thatΔpilU cells displayed increased TFP numbers but reduced cell movement and delayed microcolony ***,beyond affecting the twitching motility,ΔpilU cells formed a thick multilayered colony edge on semi-solid sur-faces,slowing colony ***-cell collision responses changed from touch-turn dominance in wild type to touch-upright dominance inΔpilU,affecting colony morphology and ***ﬁndings expand our understanding of PilU'sphysiological roles and provide potential targets for developing strategies to control *** bioﬁlm formation andvirulence.

关键词： colony expansion pilU Pseudomonas aeruginosa twitching type IV pili

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Exploring the abundance and influencing factors of antimicrobial resistance genes in manure plasmidome from swine farms

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Journal of Environmental sciences 2023年第2期124卷 462-471页

作者： Zunfang Tu Junrui Shui Jinxin Liu Hongmei Tuo Haoyu Zhang Cong Lin Jingyi Feng Yuxuan Feng Wen Su Anyun Zhang Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio-Resource and Eco-Environment of Ministry of EducationCollege of Life SciencesSichuan UniversityChengdu 610065China Laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Plasmids play a critical role in the dissemination of antimicrobial resistance genes(ARGs),however,a systematical understanding of ARGs originated from plasmids in swine production is currently ***,quantitative polymerase chain reaction was applied to determine the prevalence of ten ARGs and the class1 integron gene intI1 of plasmid source in swinemanure from 44 farms in Sichuan,Hubei and Hebei provinces,*** assayed ARGs were observed in plasmid DNA samples,and the average absolute abundance of aac(6’)-Ib-cr,blaNDM,blaCTX-M,optrA,ermB,floR,mcr-1,qnrS,tetM,sul1 and intI1 were 7.09,2.90,4.67,6.62,7.55,7.14,4.08,4.85,7.16,7.11 and 8.07 of 10 log copies/gram,***1 showed a high correlation(r>0.8,P<0.01)with the abundance of aac(6’)-Ib-cr and sul1 in swine ***,the farm scale(i.e.,herd population)and geographical location were not found to be critical factors influencing the absolute abundance of ARGs of plasmid DNA in swine ***,the concentrations of florfenicol,Cu,Zn,Fe,total phosphorus(TP)and total potassium(TK)demonstrated a significant correlation with the abundance of several ***,Cu and Zn had high correlations with optrA and blaCTX-M,*** results demonstrated that antibiotics,heavy metals and environmental nutrients are likely jointly contributing to the long-term persistence of ARGs in swine *** study provides insights into the abundance and influencing factors of ARGs from swine manure,which is of significance for assessing and reducing the public health risks in livestock production.

关键词： Antimicrobial resistance genes Plasmid Swine manure Influencing factors

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CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water

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Journal of Pharmaceutical Analysis 2024年第10期14卷 1505-1513页

作者： Yue Liu Taimei Cai Sen Chen Tao Wen Hailong Peng Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province School of Life ScienceJiangxi Science and Technology Normal UniversityNanchang330013China School of Chemistry and Chemical Engineering Nanchang UniversityNanchang330031China College of Mechanical and Intelligent Manufacturing Central South University of Forestry and TechnologyChangsha410004China

The overuse of antibiotics has led to the severe contamination of water bodies,posing a considerable hazard to human ***,the development of an accurate and rapid point-of-care testing(POCT)platform for the quantitative detection of antibiotics is *** this study,Cerium oxide(CeO_(2))and Ferrosoferric oxide(Fe_(3)O_(4))nanoparticles were simultaneously encapsulated into N-doped nanofibrous carbon microspheres to form of a novel nanozyme(CeFe-NCMzyme)with a porous structure,high surface area,and N-doped carbon material properties,leading to a considerable enhancement of the peroxidase(POD)-like activity compared with that of the CeO_(2)or Fe3O4 nanoparticles *** POD-like activity of CeFe-NCMzyme can be quenched using L-Cysteine(Cys)and subsequently restored by the addition of a quinolone antibiotic(norfloxacin,NOR).Therefore,CeFe-NCMzyme was used as a colorimetric sensor to detect NOR via an“On-Off”model of POD-like *** sensor possessed a wide linear range of 0.05–20.0μM(R^(2)=0.9910)with a detection limit of 35.70 ***,a smartphone-assisted POCT platform with CeFe-NCMzyme was fabricated for quantitative detection of NOR based on RGB *** the use of the POCT platform,a linear range of 0.1–20.0μM and a detection limit of 54.10 nM were *** spiked recoveries in the water samples were ranged from 97.73%to 102.01%,and the sensor exhibited good accuracy and acceptable *** study provides a portable POCT platform for the on-site and quantitative monitoring of quinolone antibiotics in real samples,particularly in resource-constrained settings.

关键词： CeO_(2)nanoparticles Peroxidase-like activity Nanofibrous carbon microspheres Point-of-care testing Norfloxacin

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