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Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

National science Review 2024年第5期11卷 365-384页

作者： Erhu Xiong Pengfei Liu Ruijie Deng Kaixiang Zhang Ronghua Yang Jinghong Li Key Laboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of EducationCollege of Chemistry and Chemical Engineering Hunan Normal University College of Biomass Science and Engineering Healthy Food Evaluation Research CenterSichuan University School of Pharmaceutical Sciences Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases Zhengzhou University Department of Chemistry Center for Bioanalytical Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry& Chemical BiologyTsinghua University Beijing Institute of Life Science and Technology

Single-nucleotide variants(SNVs) are the most common type variation of sequence alterations at a specific location in the genome, thus involving significant clinical and biological information. The assay of SNVs has engaged great awareness, because many genome-wide association studies demonstrated that SNVs are highly associated with serious human diseases. Moreover, the investigation of SNV expression levels in single cells are capable of visualizing genetic information and revealing the complexity and heterogeneity of single-nucleotide mutation-related diseases. Thus, developing SNV assay approaches in vitro, particularly in single cells, is becoming increasingly in demand. In this review, we summarized recent progress in the enzyme-free and enzyme-mediated strategies enabling SNV assay transition from sensing interface to the test tube and single cells, which will potentially delve deeper into the knowledge of SNV functions and disease associations, as well as discovering new pathways to diagnose and treat diseases based on individual genetic profiles. The leap of SNV assay achievements will motivate observation and measurement genetic variations in single cells, even within living organisms, delve into the knowledge of SNV functions and disease associations, as well as open up entirely new avenues in the diagnosis and treatment of diseases based on individual genetic profiles.

关键词： single-nucleotide variant point mutation in vitro enzyme-free assay enzyme-mediated assay

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Regulating metalloimmunology with nanomedicine for cancer therapy

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Nano Research 2023年第12期16卷 13164-13181页

作者： Saibo Ma Lin Chen Muhammad Hamza Jing Chang Motao Zhu College of Marine Life Science Ocean University of ChinaQingdao 266003China CAS Key Laboratory for Biomedical Effects of Nanomaterials&Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266235China University of Chinese Academy of Sciences Beijing 100049China

Metals are essential components of both micronutrients and macronutrients in living organisms and are involved in a variety of immune processes in the forms of free ions or protein-coupled complexes(metalloproteins).Multiple aspects of the immune system,from the structural and functional control of immune-related proteins to the cellular responses to immunotherapy,could be affected by ***,the employment of metal for the regulation of immunity,termed as metalloimmunology,is gaining interest as a prevalent and efficacious approach to combating ***,the manipulation of metalloimmunology using traditional drugs presents several challenges,including limited bioavailability,adverse effects,and a lack of targeting *** review provides an overview of the latest findings in metal and metal-regulatory therapeutic agents for the treatment of *** trace metal elements,such as iron,zinc,copper,manganese,magnesium,and calcium,as well as heavy metal drugs and their mechanisms of action,will be discussed with a particular focus on their roles in regulating the tumor-immune *** latest nanotechnology employed in the administration of metal-regulatory drugs and the design concepts for tailored therapeutic interventions will be *** concepts and information offer promising clinical possibilities of modulating cancer immunology by targeting metal metabolism.

关键词： metalloimmunology metal metabolism cancer immunotherapy nano-enabled delivery system cGAS-STING pathway ferroptosis and cuprotosis

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Myosin regulates intracellular force and guides collective cancer cell migration via the FAK-Rho/ROCK feedback loop

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Genes & Diseases 2023年第6期10卷 2199-2201页

作者： Xiang Qin Yuchen He Yixi Zhang Shun Li Tingting Li Fengming You Yiyao Liu Department of Pharmacy Personalized Drug Therapy Key Laboratory of Sichuan ProvinceSichuan Provincial People's HospitalSchool of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduSichuan 611731China TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province Hospital of Chengdu University of Traditional Chinese MedicineChengduSichuan 610072China

Breast cancerhas been one of the biggest killers of women due to its susceptibility and high *** observations show that malignant cancer cells frequently invade the surrounding normal tissue in collective rather than individual cell migration.1,2 For individual cell migration,it has been found that the Rho/ROCK signaling is upregulated and correlates with disease progression.3 Meanwhile,Rho activates myosin-ll and the actomyosin-mediated contraction creates tension within the cells.4,5 However,the roles of the activation of the Rho/RoCK signal pathway in collective cell migration and the precise mechanisms by which myosin-ll fine-tunes the contractility of the cells to allow for the reorganization of the cytoskeleton that drives collective cell migration,remain *** study investigated whether the high cellular contractility could activate the inside-out signal transductions and how did the intracellular force contribute to the collective cell migration.

关键词： cancer collective migration

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Health Assessment of Split Torque Transmission System Using Improved Generative Adversarial Networks

Health Assessment of Split Torque Transmission System Using ...

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TEPEN International Workshop on Fault Diagnostics and Prognostics, TEPEN-IWFDP 2024

作者： Wang, Zhi Hu, Niaoqing Xu, Fujian Yang, Yi Hu, Lei Laboratory of Science and Technology on Integrated Logistics Support NUDT Changsha410073 China Beijing Tangzhi Technology Development Co. LTD Beijing100043 China AECC Hunan Aviation Powerplant Research Institute Zhuzhou412002 China Aero-Engine Vibration Technology in Aeronautical Science Key Laboratory Zhuzhou412002 China Hunan Provincial Key Laboratory of Health Maintenance for Mechanical Equipment Hunan University of Science and Technology Xiangtan411201 China

ISBN: (纸本)9783031694820

Split-torque transmission system (STTS) is a new type of helicopter main rotor transmission. It has the characteristics of light quality, fewer transmission stages, high transmission efficiency, high reliability, low noise and so on. It is of great significance to carry out health detection and assessment for helicopter STTS. In order to solve the lack of fault data and priori knowledges, this paper proposes a framework for STTS anomaly detection and health assessment based on improved generative adversarial networks (IGAN). The framework is validated with fault simulation data of a STTS. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.

关键词： Generative adversarial networks

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Double spatial confinement on ruthenium nanoparticles inside carbon frameworks as durable catalysts for a quasi-solid-state Li–O_(2) battery

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Carbon Energy 2023年第8期5卷 114-125页

作者： Meiling Wang Ying Yao Feiyang Yang Zhenwu Tang Jingjie Ren Cunzhong Zhang Feng Wu Xiangke Wang Beijing Key Laboratory of Environmental Science and Engineering School of Materials Science&EngineeringBeijing Institute of TechnologyBeijingChina Beijing Institute of Technology Chongqing Innovation Center ChongqingChina College of Life and Environmental Sciences Minzu University of ChinaBeijingChina College of Environmental Science and Engineering North China Electric Power UniversityBeijingChina

The rational design of large-area exposure,nonagglomeration,and longrange dispersion of metal nanoparticles(NPs)in the catalysts is critical for the development of energy storage and conversion *** attention has been focused on modulating and developing catalyst interface contact engineering between a carbon substrate and dispersed ***,a highly dispersed ultrafine ruthenium(Ru)NP strategy by double spatial confinement is proposed,that is,incorporating directed growth of metal–organic framework crystals into a bacterial cellulose templating substrate to integrate their respective merits as an excellent electrocatalytic cathode catalyst for a quasi-solid-state Li–O_(2) *** porous carbon matrix with highly dispersed ultrafine Ru NPs is well designed and used as cathode catalysts in a Li–O_(2) battery,demonstrating a high discharge areal capacity of 6.82 mAh cm^(–2) at 0.02 mA cm^(–2),a high-rate capability of 4.93 mAh cm^(–2) at 0.2 mA cm^(–2),and stable discharge/charge cycling for up to 500 cycles(2000 h)with low overpotentials of~1.4 *** fundamental understanding of the structure–performance relationship demonstrates a new and promising approach to optimize highly efficient cathode catalysts for solid-state Li–O_(2) batteries.

关键词： bacterial cellulose cathode materials metal-organic frameworks Ru nanoparticles solid-state Li-O_(2)batteries

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VCP downstream metabolite glycerol-3-phosphate(G3P)inhibits CD8+T cells function in the HCC microenvironment

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Signal Transduction and Targeted Therapy 2025年第2期10卷 1040-1057页

作者： Cheng Cheng Qingrui Zha Linmao Sun Tianming Cui Xinyu Guo Changjian Xing Zhengxiang Chen Changyong Ji Shuhang Liang Shengwei Tao Junhui Chu Chenghui Wu Qi Chu Xuetian Gu Ning Zhang Yumin Fu Shumin Deng Yitong Zhu Jiabei Wang Yao Liu Lianxin Liu Department of Hepatobiliary Surgery Centre for Leading Medicine and Advanced Technologies of IHMThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230001China Anhui Provincial Key Laboratory of Hepatopancreatobiliary Surgery HefeiAnhui 230001China Anhui Provincial Clinical Research Center for Hepatobiliary Diseases HefeiAnhui 230001China Department of Gastrointestinal Surgery Anhui Province Key Laboratory of Hepatopancreatobiliary SurgeryThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China

CD8+T cells within the tumor microenvironment(TME)are often functionally impaired,which limits their ability to mount effective anti-tumor ***,the molecular mechanisms behind this dysfunction remain incompletely ***,we identified valosin-containing protein(VCP)as a key regulator of CD8+T cells suppression in hepatocellular carcinoma(HCC).Our findings reveal that VCP suppresses the activation,expansion,and cytotoxic capacity of CD8+T cells both in vitro and in vivo,significantly contributing to the immunosuppressive nature of the ***,VCP stabilizes the expression of glycerol-3-phosphate dehydrogenase 1-like protein(GPD1L),leading to the accumulation of glycerol-3-phosphate(G3P),a downstream metabolite of *** accumulated G3P diffuses into the TME and directly interacts with SRC-family tyrosine kinase LCK,a critical component of the T-cell receptor(TCR)signaling pathway in CD8+T *** interaction heightens the phosphorylation of Tyr505,a key inhibitory residue,ultimately reducing LCK activity and impairing downstream TCR ***,CD8+T cells lose their functional capacity,diminishing their ability to fight against ***,we demonstrated that targeting VCP in combination with anti-PD1 therapy significantly suppresses HCC tumor growth and restores the anti-tumor function of CD8+T cells,suggesting synergistic therapeutic *** findings highlight a previously unrecognized mechanism involving VCP and G3P in suppressing T-cell-mediated immunity in the TME,positioning VCP as a promising upstream target for enhancing immunotherapy in HCC.

关键词： molecular mechanisms valosin containing protein glycerol phosphate dehydrogenase protein hepatocellular carcinoma CD T cells hepatocellular carcinoma hcc our immunosuppression tumor microenvironment tme

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Temporal Fractal Pattern Recognition for Visual Working Memory Tasks Based on FMRI 21

Temporal Fractal Pattern Recognition for Visual Working Memo...

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21st International Computer Conference on Wavelet Active Media technology and Information Processing, ICCWAMTIP 2024

作者： Jiayu, Li Ronglong, Xiong Ling, Li Center for Psychiatry and Psychology School of Life Science and Technology University of Electronic Science and Technology of China MOE Key Lab for Neuroinformation High-Field Magnetic Resonance Brain Imaging Key Laboratory of Sichuan Province Chengdu610054 China

ISBN: (纸本)9798331519254

Fractal theory is more sensitive than Pearson correlation in detecting a slight variation between cognitive states. Therefore, to uncover fractal patterns induced by visual working memory under specific category representation tasks and varying levels of load, and to establish a better classification model, this study employed the typical fractal indicators, the Hurst exponent and wavelet entropy, which characterize system (or signal) persistence and complexity. Based on this framework, we developed a multi-class machine learning model with improved classification performance. © 2024 IEEE.

关键词： Fractals

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Conductive fibers for biomedical applications

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Bioactive Materials 2023年第4期22卷 343-364页

作者： Leqian Wei Shasha Wang Mengqi Shan Yimeng Li Yongliang Wang Fujun Wang Lu Wang Jifu Mao Key Laboratory of Textile Science&Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai201620China Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology Donghua UniversityShanghai201620China School of Health and Life Sciences University of Health and Rehabilitation SciencesQingdao CityShandong Province266071China

Bioelectricity has been stated as a key factor in regulating cell activity and tissue function in electroactive ***,various biomedical electronic constructs have been developed to interfere with cell behaviors to promote tissue regeneration,or to interface with cells or tissue/organ surfaces to acquire physiological status via electrical *** from the outstanding advantages of flexibility,structural diversity,customizable mechanical properties,and tunable distribution of conductive components,conductive fibers are able to avoid the damage-inducing mechanical mismatch between the construct and the biological environment,in return to ensure stable functioning of such constructs during physiological ***,this review starts by presenting current fabrication technologies of conductive fibers including wet spinning,microfluidic spinning,electrospinning and 3D printing as well as surface modification on fibers and fiber *** provide an update on the biomedical applications of conductive fibers and fiber assemblies,we further elaborate conductive fibrous constructs utilized in tissue engineering and regeneration,implantable healthcare bioelectronics,and wearable healthcare *** conclude,current challenges and future perspectives of biomedical electronic constructs built by conductive fibers are discussed.

关键词： Conductive fibers Conductive biomaterials Tissue repair Implantable bioelectronics Wearable bioelectronics

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RNA-Seq uncovers endogenous NO-induced hormone signal transduction and carbon metabolism in response to PEG stress in alfalfa

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BMC Genomics 2025年第1期26卷 1-14页

作者： Zhao, Ying Zhang, Xiaofang Wang, Yizhen Ruan, Qian Wang, Baoqiang Wen, Xiaoyue Wei, Xiaohong College of Life Science and Technology Gansu Agricultural University Lanzhou 730070 China Gansu Provincial Key Laboratory of Aridland Crop Science Gansu Agricultural University Lanzhou 730070 China Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement Lanzhou 730070 China

Background: Alfalfa (Medicago sativa L.) has the benefits of high yield and nutritional value as a sustainable forage. However, the water deficit significantly limits its growth and yield performance. Nitric oxide (NO) is a signal molecule that can enhance plant tolerance. The majority of previous studies focus on the role of exogenous NO in plant tolerance. However, the underlying mechanism of endogenous NO in alfalfa drought tolerance remains largely unexplored. Results: To explore the mechanism of the endogenous NO-mediated water deficit resistance in the alfalfa, seedlings were exposed to polyethylene glycol 6000 (PEG) and NO scavenger (cPTIO). Results showed that PEG treatment significantly augmented alfalfa endogenous NO, MDA, O₂^·−, and H₂O₂ levels. In parallel, eliminating endogenous NO under PEG stress (PEG-NO) significantly diminished NO level, exacerbated MDA and reactive oxygen species accumulation, and decreased the activities of key enzymes involved in carbon fixation and TCA cycle, such as Rubisco, FBA, PDH, α-KGDH, and SDH, as well as reduced ABA and IAA content in alfalfa leaves. RNA-Seq and bioinformatics analysis suggested that endogenous NO-responsive DEGs primarily relate to carbon metabolism and hormone signal transduction. In further studies of these DEGs, we speculated that GH3, SAUR, SnRK2, and ABF genes and FBA, GAPDH, SBP, and CS are critical genes in response to endogenous NO under PEG stress. Conclusions: In summary, our study innovatively proposes a mechanism model of how endogenous NO enhances alfalfa tolerance to water deficiency at the physiological and molecular levels. The novel candidate genes can give genetic resources for the subsequent molecular-assisted breeding of drought-resistant alfalfa crops. © The Author(s) 2025.

关键词： Medicago sativa Nitric oxide TCA cycle Transcription regulation Water deficit

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Ferricyanide-Promoted Oxidative Activation and Ligation of Protein Thioacids in Neutral Aqueous Media

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CCS Chemistry 2024年第8期6卷 2031-2043页

作者： Guo-Chao Chu Lu-Jun Liang Rui Zhao Yan-Yan Guo Chun-Tong Li Chong Zuo Huasong Ai Xiao Hua Zi-Chen Li Yi-Ming Li Lei Liu NewCornerstone Science Laboratory Tsinghua-Peking Center for Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyCenter for Synthetic and Systems BiologyDepartment of ChemistryTsinghua UniversityBeijing 100084 School of Food and Biological Engineering Hefei University of TechnologyHefei 230009 Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026 Department of Chemistry University of Science and Technology of ChinaHefei 230026

Ferricyanide-promoted oxidative activation of Nacylatedα-aminothioacids for amide bond formation withα-aminonitriles was recently shown to be a plausible pathway for prebiotic peptide *** we describe the finding that by adding sodium azide and thiols,ferricyanide oxidation can elicit highly efficient and clean conversion of fully unprotected peptide or protein thioacids in neutral aqueous media to the corresponding *** transformation enables the development of ferricyanide-promoted thioacid-based native chemical ligation(NCL)as a new redox-based method for chemical protein synthesis,which does not need to change pH and is therefore operationally easy for ligation at small *** effectiveness of the ferricyanide-promoted thioacid-based NCL was illustrated by synthesis of an ISG15-modified MDA5 segment under nondenaturing conditions and synthesis of an acetylated ubiquitin(Ub)-modified histone H2A through an N-to-C sequential *** work broadens the concept of on-demand oxidative activation strategy for protein ligation and provides a new useful supplement to the repertoire of methods for chemical protein synthesis,particularly for studies on proteins carrying Ub family modifications.

关键词： chemical protein synthesis protein thioacid ferricyanide oxidation native chemical ligation ubiquitin family modifications

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