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Ferricyanide-Promoted Oxidative Activation and Ligation of Protein Thioacids in Neutral Aqueous Media

CCS Chemistry 2024年第8期6卷 2031-2043页

作者： Guo-Chao Chu Lu-Jun Liang Rui Zhao Yan-Yan Guo Chun-Tong Li Chong Zuo Huasong Ai Xiao Hua Zi-Chen Li Yi-Ming Li Lei Liu NewCornerstone Science Laboratory Tsinghua-Peking Center for Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyCenter for Synthetic and Systems BiologyDepartment of ChemistryTsinghua UniversityBeijing 100084 School of Food and Biological Engineering Hefei University of TechnologyHefei 230009 Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026 Department of Chemistry University of Science and Technology of ChinaHefei 230026

Ferricyanide-promoted oxidative activation of Nacylatedα-aminothioacids for amide bond formation withα-aminonitriles was recently shown to be a plausible pathway for prebiotic peptide *** we describe the finding that by adding sodium azide and thiols,ferricyanide oxidation can elicit highly efficient and clean conversion of fully unprotected peptide or protein thioacids in neutral aqueous media to the corresponding *** transformation enables the development of ferricyanide-promoted thioacid-based native chemical ligation(NCL)as a new redox-based method for chemical protein synthesis,which does not need to change pH and is therefore operationally easy for ligation at small *** effectiveness of the ferricyanide-promoted thioacid-based NCL was illustrated by synthesis of an ISG15-modified MDA5 segment under nondenaturing conditions and synthesis of an acetylated ubiquitin(Ub)-modified histone H2A through an N-to-C sequential *** work broadens the concept of on-demand oxidative activation strategy for protein ligation and provides a new useful supplement to the repertoire of methods for chemical protein synthesis,particularly for studies on proteins carrying Ub family modifications.

关键词： chemical protein synthesis protein thioacid ferricyanide oxidation native chemical ligation ubiquitin family modifications

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金属纳米伴侣界面金属原子对淀粉样蛋白纤维化的调控作用

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science China Materials 2024年第7期67卷 2345-2354页

作者：李虎高冠斌余良翀张子俊张斌邓昱周慕庆雪沈雷孙涛垒 State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Hubei Key Laboratory of Nanomedicine for Neurodegenerative Diseases School of ChemistryChemical Engineering and Life ScienceWuhan University of TechnologyWuhan 430070China

合成具有生物分子伴侣功能的金属纳米伴侣作为调节蛋白质构象的候选物具有良好的应用前景.尽管对金属纳米伴侣已有广泛研究,但其分子中界面金属原子对蛋白质构象的调控作用及其机制尚不清楚,这严重阻碍了对高性能金属纳米伴侣的开发.本... 详细信息

合成具有生物分子伴侣功能的金属纳米伴侣作为调节蛋白质构象的候选物具有良好的应用前景.尽管对金属纳米伴侣已有广泛研究,但其分子中界面金属原子对蛋白质构象的调控作用及其机制尚不清楚,这严重阻碍了对高性能金属纳米伴侣的开发.本研究以相同配体巯基丙酸(MPA)修饰的金纳米团簇Au_(25)为模型,通过界面掺杂金属原子(Cu、Cd、Ag)精准制备出了三种单原子掺杂的纳米伴侣,分别是Au_(24)Cu_(1)、Au_(24)Cd_(1)和Au_(24)Ag_(1),系统地研究了Au_(25)、Au_(24)Cu_(1)、Au_(24)Cd_(1)和Au_(24)Ag_(1)四种纳米伴侣对Aβ40折叠与纤维化的调控作用.全原子分子动力学模拟结果表明,界面掺杂金属原子(Cu、Cd、Ag)可通过范德华力和静电相互作用等弱相互作用影响与之接触的Aβ40单体的折叠.等温滴定量热分析结果进一步表明,Au_(24)Cu_(1)与Aβ40的相互作用分别是Au_(24)Cd_(1)、Au_(24)Ag_(1)和Au_(25)的1.76、2.09、16.67倍.这些不同的相互作用会导致金属纳米伴侣对Aβ折叠与纤维化的不同调节作用,四种金属纳米伴侣抑制Aβ折叠与纤维化效率依次为Au_(24)Cu1>Au_(24)Cd_(1)>Au_(24)Ag_(1)>Au_(25).本文提供了对金属纳米团簇界面金属原子如何发挥作用的微观分子见解,并为通过操纵界面金属原子来定制金属纳米伴侣提供了基础研究数据.

关键词： customizing nano-chaperone nano-interface gold-nanoclusters peptide amyloidosis

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Index model equation analysis:A case study of the risk and source of inorganic contaminants in roadside uncontaminated soil of the Egi oil producing area,Niger Delta

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International Journal of Sediment Research 2023年第6期38卷 891-900页

作者： Elechi Owhoeke Asmat Ali Okorondu Justin Nnaemeka Kingsley John Orie Julius Nkeonyeasua Ehiwario Abdur Rashid Department of Industrial Chemistry/Petrochemical Technology School of Science Laboratory TechnologyUniversity of Port Harcourt School of Environmental Studies China University of Geosciences State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Department of Environmental Management Federal University of Technology Owerri Department of Pure and Industrial Chemistry University of Port Harcourt Department of Microbiology Faculty of Life ScienceAmbrose Alli University

This study examined the sources,health risk and pollution status of selected inorganic contaminants such as iron（Fe）,nickel（Ni）,cadmium（Cd）,cobalt（Co）,arsenic（As）,manganese（Mn）,lead（Pb）,copper（Cu）,zinc（Zn）,and chromium（Cr） in representative roadside uncontaminated soil（n=72） from the Egi crude oil mining areas（Oboburu,Obagi,and Ogbogu）,Niger Delta,Nigeria,using a combination of multiple models(Principle component analysis（PCA）,correlation analysis,geo-accumulation index（Igeo）,contamination factor（CF）,enrichment factor（EF）,pollution load index（PLI）,cancer risk（CR）,hazard quotient（HQ）,and metal daily intake（MDIm）).A random sampling method was applied in the sampling and analysis using inductively coupled plasma mass *** result revealed that the mean（mg/kg） for Oboburu,was the highest for Fe（89.95 ± 28.83） and Mn（26.12 ± 7.642）,whereas Obagi,had less Fe（28.90 ± 7.601） and Mn（16.80 ± 5.021）,and Ogbogu,Fe（44.67 ± 28.15） and Pb（19.38± 8.731） was the second highest heavy *** order of contamination was F> Mn> Zn> Pb> Cu> Cr> Ni> Cd> *** Igeo and CF had cadmium as a metal of *** contamination level was low from the PLI *** EF revealed anthropogenic sources for all the metals except for Ni,which was *** correlations at p <0.05 and p <0.01 showed much positive significance amongst the *** of the cumulative percentages,loading percentages,and eigenvalues from the regression plot gave a coefficients of determination（r2） of 0.9936,0.9995,and 0.9994,*** PCA indicated that Cd and Ni were of anthropogenic origin;Mn,Fe,and Co were biogenic or natural,and Zn and Pb were of mixed *** CR assessment indicated a possible cancer risk due to Cr,Cd,and Ni,and the rest of elements have no carcinogenic *** findings suggest there should be regular monitoring,source control,and integrated environmental management of the study area.

关键词： Soil Source identification Health risk Carcinogenicity Variability

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Effect of Nitrogen and Sulfur Co‑Doped Graphene on the Electrochemical Hydrogen Storage Performance of Co_(0.9)Cu_(0.1)Si Alloy

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Acta Metallurgica Sinica(English Letters) 2023年第6期36卷 1023-1037页

作者： Wenhao Fan Jianxun Zhao Dayong Liu Qingcheng Liang Wanqiang Liu Qingshuang Wang Heng Liu Peng Chen Shang Gao Xinlong Bao Yong Cheng Xinwei Wang Xin Guo School of Materials Science and Engineering Changchun University of Science and TechnologyChangchun 130022China Engineering Research Center of Optoelectronic Functional Materials Ministry of EducationChangchun 130022China College of Electronic Science and Engineering Jilin UniversityChangchun 130022China School of Life Science and Technology Changchun University of Science and TechnologyChangchun 130022China Research Center for Nanotechnology Changchun University of Science and TechnologyChangchun 130022China Research Institute of Changchun University of Science and Technology in Chongqing Chongqing 401120China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryCASChangchun 130022China

Co_(0.9)Cu_(0.1)Si alloy was prepared by mechanical alloying ***-doped graphene(NG)and nitrogen–sulfur codoped graphene(NSG)were prepared by hydrothermal method.5 wt%graphene oxide,NG and NSG were doped into Co_(0.9)Cu_(0.1)Si alloy,respectively,by ball milling to improve the electrochemical hydrogen storage performance of the composite material.X-ray diffraction and scanning electron microscopy were used to characterize the structure and morphology of the composite material,and the LAND battery test system and three-electrode battery system were used to test the electrochemical performance of the composite *** composite material showed better discharge capacity and better cycle stability than the pristine *** addition,in order to study the optimal ratio of NSG,3%,5%,7%and 10%of NSG were doped into Co_(0.9)Cu_(0.1)Si alloy,***_(0.9)Cu_(0.1)Si alloy doped with 5%NSG had the best performance among all the *** best discharge capacity was 580.1 mAh/g,and its highest capacity retention rate was 64.1%.The improvement in electrochemical hydrogen storage performance can be attributed to two *** the one hand,the electrocatalytic performance of graphene is improved by co-doping nitrogen and sulfur,on the other hand,graphene has excellent electrical conductivity.

关键词： Co_(0.9)Cu_(0.1)Si alloy Nitrogen-sulfur co-doped graphene(NSG) Composite material Electrochemical hydrogen storage

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Structural basis for histone H3 recognition by NASP in Arabidopsis

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Journal of Integrative Plant Biology 2022年第12期64卷 2309-2313页

作者： Yanhong Liu Liu Chen Na Wang Baixing Wu Hongyu Bao Hongda Huang Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Department of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China School of Life Science and Technology Harbin Institute of TechnologyHarbin 150080China

The structural basis for histone recognition by the histone chaperone nuclear autoantigenic sperm protein(NASP)remains largely ***,we showed that Arabidopsis thaliana AtNASP is a monomer and displays robust nucleosome assembly activity in *** the structure of AtNASP complexed with a histone H3α3 peptide revealed a binding mode that is conserved in human *** recognizes the H3 N-terminal region distinct from human ***,AtNASP forms a co-chaperone complex with ANTI-SILENCING FUNCTION 1 ASF1 by binding to the H3 Nterminal ***,we deciphered the structure of AtNASP and the basis of the AtNASP-H3 interaction.

关键词： Arabidopsis NASP ASF1 crystal structure epigenetics histone chaperone histone H3

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Lateral interaction by Laplacian‐based graph smoothing for deep neural networks

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CAAI Transactions on Intelligence technology 2023年第4期8卷 1590-1607页

作者： Jianhui Chen Zuoren Wang Cheng‐Lin Liu Institute of Neuroscience State Key Laboratory of NeuroscienceCenter for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghaiChina State Key Laboratory of Multimodal Artificial Intelligence Systems Institute of Automation of Chinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina School of Future Technology University of Chinese Academy of SciencesBeijingChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina

Lateral interaction in the biological brain is a key mechanism that underlies higher cognitive *** self‐organising map(SOM)introduces lateral interaction in a general form in which signals of any modality can be *** approaches directly incorporate SOM learning rules into neural networks,but incur complex operations and poor *** efficient way to implement lateral interaction in deep neural networks is not well *** use of Laplacian Matrix‐based Smoothing(LS)regularisation is proposed for implementing lateral interaction in a concise *** authors’derivation and experiments show that lateral interaction implemented by SOM model is a special case of LS‐regulated k‐means,and they both show the topology‐preserving *** authors also verify that LS‐regularisation can be used in conjunction with the end‐to‐end training paradigm in deep auto‐***,the benefits of LS‐regularisation in relaxing the requirement of parameter initialisation in various models and improving the classification performance of prototype classifiers are ***,the topologically ordered structure introduced by LS‐regularisation in feature extractor can improve the generalisation performance on classification ***,LS‐regularisation is an effective and efficient way to implement lateral interaction and can be easily extended to different models.

关键词： artificial neural networks biologically plausible Laplacian‐based graph smoothing lateral interaction machine learning

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Test and simulation of emergency flotation system buoy anti-overturning method 2

Test and simulation of emergency flotation system buoy anti-...

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2023 2nd International Conference on Aerospace and Control Engineering, ICoACE 2023

作者： Yang, Li Huang, Yanping Sun, Libing He, Fan Zhu, Yuanchun Emergency Rescue Technology R&D Department Hangyu Life-saving Equipment Co. Ltd. Xiangyang441100 China Key Laboratory of Aviation Science and Technology for Aviation Protection and Life-saving Technology Xiangyang441100 China

The helicopter emergency flotation system can provide flotation capability after the helicopter accidentally falls into the water. During a test of a helicopter float in a submerged state, it was found that the vertical distance between the highest point of the contact point of the float and the aircraft and the lowest point of the cabin door was 0.59 m, which seriously hindered the entry and exit of the crew from the cabin door. To reduce the adverse effects of the pontoon flipping up on the occupants entering and exiting the hatch, it is envisaged to use restraint belts, add anti-rollover airbags, and design the anti-rollover airbags into triangles and saddle shapes respectively. Through theoretical analysis, the simulation method eliminated the triangle method for the connecting belt and the anti-rollover airbag and selected the saddle-shaped anti-rollover airbag for floating and immersion tests. The test results met expectations. The highest point of the contact point between the float and the aircraft was the lowest point of the cabin door. The points did not interfere in the vertical direction, and this method was finally chosen to be applied to the buoy to suppress the upturn of the buoy. © Published under licence by IOP Publishing Ltd.

关键词： Flotation

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Regulating crystallization and retarding oxidation in Sn-Pb perovskite via 1D cation engineering for high performance all-perovskite tandem solar cells

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Journal of Energy Chemistry 2025年第1期100卷 646-652页

作者： Ranran Liu Xin Zheng Zaiwei Wang Miaomiao Zeng Chunxiang Lan Shaomin Yang Shangzhi Li Awen Wang Min Li Jing Guo Xuefei Weng Yaoguang Rong Xiong Li Michael Grätzel Center for Mesoscopic Solar Cells Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074HubeiChina Institute of Technology for Carbon Neutrality Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055GuangdongChina School of Chemistry Chemical Engineering and Life SciencesState Key Laboratory of Advanced Technology for Materials Synthesis and ProcessingWuhan University of Technology430070HubeiChina Suzhou Institute of Nano-Tech and Nano-Bionics(SINANO) Chinese Academy of SciencesSuzhou 215123JiangsuChina School of Physics and Optoelectronic Engineering Hainan UniversityHaikou 570228HainanChina

All-perovskite tandem solar cells have the potential to surpass the theoretical efficiency limit of single junction solar cells by reducing thermalization ***,the challenges encompass the oxidation of Sn^(2+)to Sn^(4+)and uncontrolled crystallization kinetics in Sn-Pb perovskites,leading to nonradiative recombination and compositional heterogeneity to decrease photovoltaic efficiency and operational ***,we introduced an ionic liquid additive,1-ethyl-3-methylimidazolium iodide (EMIMI) into Sn-Pb perovskite precursor to form low-dimensional Sn-rich/pure-Sn perovskites at grain boundaries,which mitigates oxidation of Sn^(2+)to Sn^(4+)and regulates the film-forming dynamics of Sn/Pb-based perovskite *** optimized single-junction Sn-Pb perovskite devices incorporating EMIMI achieved a high efficiency of 22.87%.Furthermore,combined with wide-bandgap perovskite sub-cells in tandem device,we demonstrate 2-terminal all-perovskite tandem solar cells with a power conversion efficiency of 28.34%,achieving improved operational stability.

关键词： All-perovskite tandem solar cells Sn-Pb perovskite 1D Regulated crystallization Antioxidation

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Enforcing energy consumption promotes microbial extracellular respiration for xenobiotic bioconversion

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Environmental Microbiology 2023年第12期25卷 2943-2957页

作者： Liang, Zi-Han Sun, Hong Li, Yang Hu, Anyi Tang, Qiang Yu, Han-Qing Department of Environmental Science and Technology University of Science and Technology of China Hefei China CAS Key Laboratory of Urban Pollutant Conversion School of Life Sciences University of Science and Technology of China Hefei China CAS Key Laboratory of Urban Pollutant Conversion Institute of Urban Environment Chinese Academy of Sciences Xiamen China

Extracellular electron transfer (EET) empowers electrogens to catalyse the bioconversion of a wide range of xenobiotics in the environment. Synthetic bioengineering has proven effective in promoting EET output. However, conventional strategies mainly focus on modifications of EET-related genes or pathways, which leads to a bottleneck due to the intricate nature of electrogenic metabolic properties and intricate pathway regulation that remain unelucidated. Herein, we propose a novel EET pathway-independent approach, from an energy manipulation perspective, to enhance microbial EET output. The Controlled Hydrolyzation of ATP to Enhance Extracellular Respiration (CHEER) strategy promotes energy utilization and persistently reduces the intracellular ATP level in Shewanella oneidensis, a representative electrogenic microbe. This approach leads to the accelerated consumption of carbon substrate, increased biomass accumulation and an expanded intracellular NADH pool. Both microbial electrolysis cell and microbial fuel cell tests exhibit that the CHEER strain substantially enhances EET capability. Analysis of transcriptome profiles reveals that the CHEER strain considerably bolsters biomass synthesis and metabolic activity. When applied to the bioconversion of model xenobiotics including methyl orange, Cr(VI) and U(VI), the CHEER strain consistently exhibits enhanced removal efficiencies. This work provides a new perspective and a feasible strategy to enhance microbial EET for efficient xenobiotic conversion. © 2023 Applied Microbiology International and John Wiley & Sons Ltd.

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Disease prediction by network information gain on a single sample basis

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Fundamental Research 2025年第1期5卷 215-227页

作者： Jinling Yan Peiluan Li Ying Li Rong Gao Cheng Bi Luonan Chen School of Mathematics and Statistics Henan University of Science and TechnologyLuoyang 471023China Key Laboratory of Information Fusion Technology of Ministry of Education School of AutomationNorthwestern Polytechnical UniversityXi'an 710072China Longmen Laboratory Luoyang 471003China Key Laboratory of Systems Biology Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang Province Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310024China Guangdong Institute of Intelligence Science and Technology Zhuhai 519031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

There are critical transition phenomena during the progression of many *** critical transitions are usually accompanied by catastrophic disease deterioration,and their prediction is of significant importance for disease prevention and ***,predicting disease deterioration solely based on a single sample is a difficult *** this study,we presented the network information gain(NIG)method,for predicting the critical transitions or disease state based on network flow entropy from omics data of each *** can not only efficiently predict disease deteriorations but also detect their dynamic network biomarkers on an individual basis and further identify potential therapeutic *** numerical simulation demonstrates the effectiveness of ***,our method was validated by successfully predicting disease deteriorations and identifying their potential therapeutic targets from four real omics datasets,i.e.,an influenza dataset and three cancer datasets.

关键词： Tipping point Network information gain Dynamic network biomarker Disease prediction Network flow entropy Drug target

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