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SSRN 2024年

作者： Yan, Yong Li, Tongxian Oliva-Ramirez, Manuel Zhao, Yuguo Wang, Shuo Chen, Xin Wang, Dong Schaaf, Peter Wang, Xiayan Guo, Guangsheng Center of Excellence for Environmental Safety and Biological Effects Beijing Key Laboratory for Green Catalysis and Separation Department of Chemistry College of Chemistry and Life Sciences Beijing University of Technology Beijing100124 China Department of Chemical Engineering and Technology College of Materials Science and Engineering Beijing University of Technology Beijing100124 China Chair Materials for Electronics Institute of Materials Engineering Institute of Micro- and Nanotechnologies MarcoNano® TU Ilmenau Gustav-Kirchhoff-Str. 5 Ilmenau98693 Germany College of Chemistry and Chemical Engineering Southwest Petroleum University Chengdu610500 China Minzu University of China Beijing100081 China

The development of efficient strategies to tune the CO2RR selectivity of Cu-based catalytic interfaces, especially on specific domains, such as Cu (200) facets with high activity toward competitive hydrogenation evolution reaction (HER), remains a challenging task. In this work, Cu-based catalytic layers with thiocyanate (-SCN), cyanide (-CN), or ethylenediamine (-NH2R) coordination linkages are prepared on Cu nanocolumns arrays (Cu NCAs) with predominant (200) exposed facets. The selectivity of Cu NCAs toward HCOOH, C2H4, and C1 mixture (HCOOH and CO) products can be effectively tuned by varying the types of ligand. The coordination of these ligands induces more Cu+ species and inhibits the adsorption of H* on the Cu (200) facet, leading to enhanced CO2RR performance and substantially suppressing the competitive HER. In situ spectroscopic studies reveal Cu-SCN, Cu-CN, and Cu-NH2R exhibits more reasonable adsorption energy toward *OCHO, *CO, and *COOH intermediates, promoting the HCOOH, C2H4, and C1 mixture generation, respectviely. © 2024, The Authors. All rights reserved.

关键词： Ligands

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Exploring the Effect and Mechanism of Benzalkonium Bromide on Short Chain Fatty Acid Production from Waste Activated Sludge

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SSRN 2023年

作者： Wang, Hongjie Li, Hang Zhu, Lei Yang, Xianglong Zhang, Qiushuo Wang, Yali Wang, Dongbo Hebei Key Laboratory of close-to-Nature restoration technology of wetlands School of Eco-Environment Hebei University Baoding071002 China School of life science Hebei university Baoding071002 China Institute of Xiong’an New Area Hebei University Baoding071002 China College of Environmental Science and Engineering Hunan University Changsha410082 China Ministry of Education Changsha410082 China

Quaternary ammonium compounds (QACs) was frequently detected in wastewater treatment plants and leads to potential toxicity to the related biological processes. In this study, the effect of benzalkonium bromide (BK) on anaerobic sludge fermentation process for short chain fatty acid (SCFAs) production was investigated. Batch experiments indicated that BK exposure significantly enhanced the SCFAs production from anaerobic fermentation sludge and the maximum concentration of total SCFAs increased from 474.40 ± 12.35 mg/L to 916.42 ± 20.35 mg/L with BK increasing from 0 to 8.69 mg/g VSS. Mechanism exploration exhibited that the presence of BK enhanced much more bioavailable organic matters release but little affected on hydrolysis, acidification, and methanogenesis. Microbial community investigation revealed that BK exposure importantly enhanced the relative abundances of hydrolytic-acidifying bacteria and the metabolic pathways and functional genes for sludge lysis were also increased. This work further supplement the information for environmental toxicity of emerging pollutants. © 2023, The Authors. All rights reserved.

关键词： Fatty acids

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Trispecific killer engager 161519 enhances natural killer cell function and provides anti-tumor activity against CD19-positive cancers

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Cancer Biology & Medicine 2020年第4期17卷 1026-1038页

作者： Ying Cheng Xiaodong Zheng Xuefu Wang Yongyan Chen Haiming Wei Rui Sun Zhigang Tian Haoyu Sun Hefei National Laboratory for Physical Sciences at Microscale The CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Sciences and MedicineInstitute of ImmunologyUniversity of Science and Technology of ChinaHefei 230027China Research Unit of NK Cell Study Chinese Academy of Medical SciencesBeijing 100864China

Objective:Natural killer(NK)cells have gained considerable attention due to their potential in treating"cold tumors,"and are therefore considered as one of the new strategies for curing cancer,by using worldwide development of their new possibilities and interventions with NK cell-related therapeutic ***:We constructed a trispecific killer engager(TriKE)consisting of anti-CD16,IL-15,and *** TriKE was designed to attract CD19^(+)tumor cells to CD16^(+)NK cells,whereas IL-15 sustained the proliferation,development,and survival of NK ***:Treatment with 161519 TriKE in the presence of CD19^(+)targets upregulated expression of CD69,CD107 a,TRAIL,IFN-γ,and TNF-α in NK cells,and significantly improved the proliferation and cytotoxicity of NK *** cells"armed"with 161519 TriKE showed stronger cytolysis against CD19+targets compared with that of"unarmed"NK cells.A preclinical model of B-cell lymphoma in human peripheral blood mononuclear cell-reconstituted xenograft mice showed significant inhibition of tumor growth and prolonged overall survival after treatment with 161519 TriKE,when compared with that in control mice or mice treated with 1619 *** use of IL-2 was a more effective treatment with 1619 BiKE,when compared with that using 161519 ***:The newly generated 161519 TriKE enhanced the proliferation,activation,cytokine secretion,and cytotoxicity of NK cells in the presence of CD19+tumor *** 161519 TriKE aided inhibition of tumor growth and prolonged the overall survival of murine xenografts,and could be used to treat CD19-positive cancers.

关键词： Trispecific antibody trispecific killer engager(TriKE) NK cell B-cell lymphoma tumor immunotherapy

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Engineered Probiotics Enable Targeted Gut Delivery of Dual Gasotransmitters for Inflammatory Bowel Disease Therapy

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Angewandte Chemie 2025年

作者： Tengfei Ma Guihai Gan Jian Cheng Zhiqiang Shen Guoying Zhang Shiyong Liu Jinming Hu Department of Pharmacy The First Affiliated Hospital of University of Science and Technology of China (USTC) Division of Life Sciences and Medicine and State Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China Hefei Anhui Province 230026 China

Inflammatory bowel disease (IBD) remains an incurable condition, often accompanied by high rates of anxiety and depression, further diminishing the quality of life of patients. Endogenous gasotransmitters, such as carbon monoxide (CO) and hydrogen sulfide (H₂S), exhibit potent anti-inflammatory and immunomodulatory effects. However, their therapeutic application is limited by challenges in targeted delivery to affected tissues. Here, we propose a novel strategy for targeted gut delivery of CO/H 2 S through engineering Escherichia coli Nissle 1917 (EcN) with CO/H 2 S-releasing copolymer (POSR) loading. This engineered probiotic (POSR@EcN) enhances EcN colonization in the intestine and enables controlled, localized release of CO/H 2 S at inflamed sites. The release of CO/H 2 S modulates inflammation, restores intestinal barrier integrity, and reshapes gut microbiota by promoting beneficial bacteria and increasing short-chain fatty acids production, effectively alleviating IBD symptoms. Notably, targeted CO/H 2 S delivery also elevates neuroprotective metabolites like indoleacetic acid and γ-aminobutyric acid, reducing neuroinflammation via the gut-brain axis and mitigating anxiety- and depression-like behaviors in IBD mice. This approach highlights the potential of EcN as a probiotic carrier for the targeted delivery of gasotransmitters, offering a promising strategy for IBD treatment.

关键词： Carbon monoxide Gut–brain axis Hydrogen sulfide Inflammatory bowel disease Probiotics

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Silver nanoparticles derived from Streptomyces sp. YJD18 with multifunctional biomedical applications

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Scientific reports 2025年第1期15卷 18139页

作者： Lijuan Niu Zihan Lin Wenzhong Hu Dongsheng Yang Haitao Shen Fei Guo Microbiology Laboratory College of Life Science & Technology Xinjiang University Xinjiang 830017 China. Zhuhai College of Science and Technology Guangdong 519040 China. Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education College of Life Sciences Shihezi University Shihezi 832003 China. Zhuhai College of Science and Technology Guangdong 519040 China. weisw2024@***.

A sustainable and energy-efficient approach was developed for the biosynthesis of silver nanoparticles (AgNPs) using a cell-free supernatant derived from Streptomyces sp. strain YJD18, an actinomycete isolated from saline soil. Optimized synthesis conditions, including pH 10 and a biomass concentration of 5 g/100 mL, facilitated the rapid formation of AgNPs. The highest yield was achieved by mixing the supernatant with silver nitrate (AgNO₃) at a 4:1 ratio, followed by incubation at 100 °C for 15 *** synthesized AgNPs exhibited multifunctional bioactivities, including antibacterial, anticancer, antioxidant, and wound-healing properties, as confirmed by UV-Vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), high-angle annular dark field-scanning transmission electron microscopy (HAADF-STEM), Fourier-transform infrared spectroscopy (FTIR), and energy-dispersive X-ray spectroscopy (EDX). The formation of AgNPs was indicated by a characteristic color change, with UV-Vis spectral analysis revealing a distinct absorption peak at 420 nm. Morphological analysis showed that the nanoparticles were predominantly spherical, with a few triangular and cylindrical structures, measuring 50-80 nm in size, with a polydispersity index (PDI) of 0.312 and a zeta potential of -24.0 mV, indicating moderate colloidal stability. The AgNPs exhibited potent antibacterial activity against both Gram-positive (G⁺) and Gram-negative (G⁻) bacteria, with synergistic effects observed in combination with standard antibiotics. Furthermore, the AgNPs displayed significant anticancer activity, with pronounced cytotoxic effects against human pulmonary carcinoma (A549) and human liver hepatocellular carcinoma (HepG2) cell lines, compared to their effects on human breast adenocarcinoma (MDA-MB-231) and human cervical carcinoma (HeLa) cells.

关键词： Streptomyces Sp. YJD18 Actinobacteria AgNPs Antibacterial activity Anticancer activity Green synthesis Wound healing

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Identification and analysis of AP2/ERF gene family in tomato underabiotic stress

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BIOCELL 2020年第4期44卷 777-803页

作者： XIAOLIN ZHU XIAOHONG WEI BAOQIANG WANG XIAN WANG MINGJUN ZHANG College of Agronomy Gansu Agricultural UniversityLanzhou730070China College of Life Science and Technology Gansu Agricultural UniversityLanzhou730070China Gansu Provincial Key Laboratory of Aridland Crop Science Lanzhou730070China Gansu Agricultural University of Plant Protection College Lanzhou730070China

AP2/ERE-type transcription factors,as a type of plant-specific transcription factors,play a key role in plant biotic and abiotic ***,they have been studied in many plants,but rarely in *** this study,we performed a genome-wide analysis of the SlAP2/ERF gene family of tomato,and finally identified 29 SlAP2/ERF genes and divided them into different *** the same time,its basic physical and chemical properties were *** also constructed phylogenetic trees with 30 Arabidopsis AP2/ERF proteins and 28 potatoes AP2/ERF proteins to ensure conservative homology between *** addition,we mapped 29 SlAP2/ERF transcription factors on 10 different chromosomes,and identified 43 responsive plant hormones,responsive light signals,tissue-specific expression and stress response elements from 2000bp upstream of the promoter region,and we analyzed conserved motifs and gene structures of SlAP2/*** tertiary structure of SlAP2/ERF protein was constructed by homology modeling,and the protein-protein interaction network was constructed based on Arabidopsis ***,the expression pattern of tomato in different tissues was studied by using gene expression database,and the expression level of tomato under abiotic stress was detected by *** results provide comprehensive information for further study of the function of the SlAP2/ERF gene family.

关键词： AP2/ERF gene family Tomato Abiotic stress Expression analysis

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A chromosome-scale genome assembly of Isatis indigotica,an important medicinal plant used in traditional Chinese medicine

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Horticulture Research 2020年第1期7卷 2565-2574页

作者： Minghui Kang Haolin Wu Qiao Yang Li Huang Quanjun Hu Tao Ma Zaiyun Li Jianquan Liu Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education&State Key Laboratory of Hydraulics&Mountain River Engineering College of Life SciencesSichuan UniversityChengdu 610065China National Key Laboratory of Crop Genetic Improvement National Center of Oil Crop Improvement(Wuhan)College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina State Key Laboratory of Grassland Agro-Ecosystem Institute of Innovation EcologyLanzhou UniversityLanzhou 730000China

Isatis indigotica(2n=14)is an important medicinal plant in *** dried leaves and roots(called Isatidis Folium and Isatidis Radix,respectively)are broadly used in traditional Chinese medicine for curing diseases caused by bacteria and viruses such as influenza and viral *** classes of compounds isolated from this species have been identified as effective *** studies based on transcriptomes revealed only a few candidate genes for the biosynthesis of these active compounds in this medicinal ***,we report a high-quality chromosome-scale genome assembly of *** with a total size of 293.88 Mb and scaffold N50=36.16 Mb using single-molecule real-time long reads and high-throughput chromosome conformation capture *** annotated 30,323 highconfidence protein-coding *** on homolog searching and functional annotations,we identified many candidate genes involved in the biosynthesis of main active components such as indoles,terpenoids,and *** addition,we found that some key enzyme-coding gene families related to the biosynthesis of these components were expanded due to tandem duplications,which likely drove the production of these major active compounds and explained why *** has excellent antibacterial and antiviral *** results highlighted the importance of genome sequencing in identifying candidate genes for metabolite synthesis in medicinal plants.

关键词： dried roots expanded

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GPS 5.0: An Update on the Prediction of Kinase-specific Phosphorylation Sites in Proteins

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Genomics, proteomics & bioinformatics 2020年第1期18卷 72-80页

作者： Chenwei Wang Haodong Xu Shaofeng Lin Wankun Deng Jiaqi Zhou Ying Zhang Ying Shi Di Peng Yu Xue (1)Key Laboratory of Molecular Biophysics of Ministry of Education Hubei Bioinformatics and Molecular Imaging Key Laboratory College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China. (1)Key Laboratory of Molecular Biophysics of Ministry of Education Hubei Bioinformatics and Molecular Imaging Key Laboratory College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China (2)Huazhong University of Science and Technology Ezhou Industrial Technology Research Institute Ezhou 436044 China. Electronic address: xueyu@***.

In eukaryotes, protein phosphorylation is specifically catalyzed by numerous protein kinases (PKs), faithfully orchestrates various biological processes, and reversibly determines cellular dynamics and plasticity. Here we report an updated algorithm of Group-based Prediction System (GPS) 5.0 to improve the performance for predicting kinase-specific phosphorylation sites (p-sites). Two novel methods, position weight determination (PWD) and scoring matrix optimization (SMO), were developed. Compared with other existing tools, GPS 5.0 exhibits a highly competitive accuracy. Besides serine/threonine or tyrosine kinases, GPS 5.0 also supports the prediction of dual-specificity kinase-specific p-sites. In the classical module of GPS 5.0, 617 individual predictors were constructed for predicting p-sites of 479 human PKs. To extend the application of GPS 5.0, a species-specific module was implemented to predict kinase-specific p-sites for 44,795 PKs in 161 eukaryotes. The online service and local packages of GPS 5.0 are freely available for academic research at http://***.

关键词： Dual-specificity kinase Group-based Prediction System Kinase-specific phosphorylation site Protein kinase Protein phosphorylation

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Label Reusing Based Graph Neural Network for Unbalanced Classification of Personalized Driver Genes in Cancer

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SSRN 2023年

作者： Wan, Han-Wen Wu, Meng-Han Zhao, Wen-Shan Cheng, Han Bi, Ying Wang, Xian-Fang Zhang, Xiang-Rui Li, Yan Guo, Wei-Feng School of Electrical and Information Engineering Zhengzhou University Zhengzhou450001 China School of International College Zhengzhou University Zhengzhou450001 China School of Life Sciences Zhengzhou University Zhengzhou450001 China The school of computer science and engineering Henan Institute of Technology Xinxiang453000 China Key Laboratory of Information Fusion Technology of Ministry of Education School of Automation Northwestern Polytechnical University Xian710072 China State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat-sen University Cancer Center Guangzhou510060 China State Key Laboratory of Intelligent Agricultural Power Equipment Zhengzhou University Luoyang471000 China

It is a big challenge to identify personalized driver genes (PDGs) for understanding tumor heterogeneity of cancer individual patients. From the perspective of machine learning, identifying PDGs is an inherent class imbalance issue due to the fewer known driver genes than most passenger ones. However, existing machine learning based methods including unsupervised and supervised learning based methods ignore the importance of limited well-established cancer tissue specific driver genes(CSDGs) for this class imbalance issue. Here we converted the PDG prediction issue to a semi-supervised classification task and a novel method (namely PersonalizedGNN) was developed to identify PDGs by using graph attention neural network and label reuse strategy in personalized gene interaction network (PGIN). PersonalizedGNN effectively utilizes the structure information of PGIN and the limited well-established CSDG information for achieving promising performance. Using the breast cancer and lung cancer datasets from The Cancer Genome Atlas, we validated our method and compared it with other advanced methods. PersonalizedGNN showed outstanding potential in identifying cancer driver genes in terms of prediction precision. Furthermore, we could discover subtype-specific de novo cancer driver genes and in vitro cell-based assays for a novel driver gene FZD7 in lung squamous cell carcinoma cells further validated the PersonalizedGNN. In summary, PersonalizedGNN offers a new effective perspective for discovering PDGs by considering information of prior known CSDGs in PGIN which help researchers understand tumor heterogeneity of cancer individual patients. © 2023, The Authors. All rights reserved.

关键词： Genes

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Concurrently Produce a Novel High-Potency Sweetener (Glycyrrhetic Acid 3-O-Mono-β-D-glucuronide) and Lignocellulolytic Enzymes using Plant Entophytic Chaetomium Globosum DX-THS3 by Solid-State Fermentation

Research Square

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Research Square 2021年

作者： Gao, Boliang Xiao, Yiwen Zhang, Qian Sun, Junru Zhang, Zhibing Zhu, Du Key Lab of Bioprocess Engineering of Jiangxi Province China College of life sciences Jiangxi Science and Technology Normal University China Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province China Jiangxi Science and Technology Normal University China

Licorice straw was used for the first time as a medium for glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) and lignocellulosic enzyme production via solid-state fermentation (SSF) of endophytic fungus Chaetomium globosum DX-THS3. Under optimal fermentation conditions, the percent conversion of glycyrrhizin reached 90% in 15 days, whereas the control needed 35 days to achieve the same result. The productivity of optimization (P=2.1 mg•g-1•day-1) was 2.33-fold that of non-optimization (P=0.9 mg•g-1•day-1). Meanwhile, high activities of filter paper enzyme (FPase) (234.6 U/g), carboxymethyl cellulase (CMCase) (29.25 U/g), xylanase (72.52 U/g), and β-glucuronidase activity (264.17 U/g) were obtained faster than those in the control during SSF. Our study provides a novel and efficient strategy for GAMG production and indicates C. globosum DX-THS3 as a potential producer of lignocellulolytic enzymes. © 2021, CC BY.

关键词： Lignocellulose

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