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Mouse MrE11-rAd50-NBS1 is needed to start and extend meiotic dNA end resection

NATUrE COMMUNICATIONS 2025年第1期16卷 1-20页

作者： Kim, Soonjoung Yamada, Shintaro Li, Tao Canasto-Chibuque, Claudia Kim, Jun Hyun Marcet-Ortega, Marina Xu, Jiaqi Eng, diana Y. Feeney, Laura Petrini, John H. J. Keeney, Scott Mem Sloan Kettering Canc Ctr Mol Biol Program New York NY 10065 USA Yonsei Univ Coll Med Inst Immunol & Immunol Dis Dept Microbiol & Immunol Seoul South Korea Tokyo Metropolitan Inst Med Sci Dept Basic Med Sci Tokyo Japan Weill Cornell Grad Sch Med Sci New York NY 10065 USA Mem Sloan Kettering Canc Ctr Howard Hughes Med Inst New York NY 10065 USA Peking Univ Third Hosp Ctr Reprod Med Dept Obstet & Gynecol State Key Lab Female Fertil Promot Beijing Peoples R China PackGene Biotech Houston TX USA AstraZeneca Translat Med Oncol R&D Barcelona Spain

Nucleolytic resection of dNA ends is critical for homologous recombination, but its mechanism is not fully understood, particularly in mammalian meiosis. Here we examine roles of the conserved MrN complex (MrE11, rAd50, and NBS1) through genome-wide analysis of meiotic resection during spermatogenesis in mice with various MrN mutations, including several that cause chromosomal instability in humans. Meiotic dSBs form at elevated levels but remain unresected if Mre11 is conditionally deleted, thus MrN is required for both resection initiation and regulation of dSB numbers. resection lengths are reduced to varying degrees in MrN hypomorphs or if MrE11 nuclease activity is attenuated in a conditional nuclease-dead Mre11 model. These findings unexpectedly establish that MrN is needed for longer-range extension of resection beyond that carried out by the orthologous proteins in budding yeast meiosis. Finally, resection defects are additively worsened by combining MrN and Exo1 mutations, and mice that are unable to initiate resection or have greatly curtailed resection lengths experience catastrophic spermatogenic failure. Our results elucidate MrN roles in meiotic dSB end processing and establish the importance of resection for mammalian meiosis.

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rail fastener defect inspection method for multi railways based on machine vision

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railway Sciences 2022年第2期1卷 210-223页

作者： Junbo Liu YaPing Huang ShengChun Wang XinXin Zhao Qi Zou XingYuan Zhang Infrastructure Inspection Research Institute China Academy of Railway Sciences Corporation LimitedBeijingChina Beijing Key Laboratory of Traffic Data Analysis and Mining Beijing Jiaotong UniversityBeijingChina Beijing R&D Centre Huawei Technologies Co LtdShenzhenChina

Purpose–This research aims to improve the performance of rail fastener defect inspection method for multi railways,to effectively ensure the safety of railway ***/methodology/approach–Firstly,a fastener region location method based on online learning strategy was proposed,which can locate fastener regions according to the prior knowledge of track image and template matching *** learning strategy is used to update the template library dynamically,so that the method not only can locate fastener regions in the track images of multi railways,but also can automatically collect and annotate fastener ***,a fastener defect recognition method based on deep convolutional neural network was *** structure of recognition network was designed according to the smaller size and the relatively single content of the fastener *** data augmentation method based on the sample random sorting strategy is adopted to reduce the impact of the imbalance of sample size on recognition ***–Test verification of the proposed method is conducted based on the rail fastener datasets of multi ***,fastener location module has achieved an average detection rate of 99.36%,and fastener defect recognition module has achieved an average precision of 96.82%.Originality/value–The proposed method can accurately locate fastener regions and identify fastener defect in the track images of different railways,which has high reliability and strong adaptability to multi railways.

关键词： rail fastener defects inspection Multi railways Image recognition deep convolutional neural network Machine vision

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Characteristics, Impacts and High-Efficiency Treatment technologies for Antibiotic residues in Aquaculture Wastewater: A review

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SSrN 2024年

作者： Wang, Tai-Yue Cheng, Ming Wang, Xiang-Yang Zhu, Hao-rui Huang, Ao Zhang, Nan Hao, Xiao-di Hao, Li-Ting Shi, Chen Sino-Dutch R&D Centre for Future Wastewater Treatment Technologies Key Laboratory of Urban Stormwater System and Water Environment Beijing University of Civil Engineering and Architecture Beijing100044 China Institute of Agricultural Environment and Sustainable Development Chinese Academy of Agriculture Sciences Beijing100081 China Key Laboratory of Urban Stormwater System and Water Environment Beijing University of Civil Engineering and Architecture Beijing100044 China

As the global aquaculture sector experiences significant growth, its societal prominence has been progressively elevated. Currently, Asia has emerged as the primary region for aquaculture, encompassing various forms of fish and shrimp farming. However, the extensive use of antibiotics in this sector has raised significant concerns regarding wastewater composition and drug residues. If these substances are released into the environment, they can pose serious risks. To mitigate the environmental impact of antibiotics, a range of removal methods have been proposed, including adsorption, photocatalysis, ozonation, and constructed wetlands. This review systematically examines the types, causes, characteristics, and environmental impacts of aquaculture wastewater while comparing different antibiotic removal techniques and conducting a life cycle assessment for each treatment process. Building on this analysis, this work anticipates future developments in aquaculture wastewater treatment. © 2024, The Authors. All rights reserved.

关键词： Aquaculture

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Phosphate recovery from Sludge-Incinerated Ash by In-Situ Adsorption with Hydrotalcite Synthesized by Metals in the Ash

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SSrN 2023年

作者： Hao, Xiaodi Wang, Xiangyang Shi, Chen Wu, Yuanyuan Sino-Dutch R&D Centre for Future Wastewater Treatment Technologies Key Laboratory of Urban Stormwater System and Water Environment Beijing University of Civil Engineering & Architecture Beijing100044 China Beijing Capital Eco-Environment Protection Group Co. Ltd. Beijing100044 China

Selective adsorption of phosphorus (P) from the acidic leachate of sludge-incinerated ash (SIA) becomes more attractive due to avoiding removing heavy metals. Especially, layered double hydroxides (LdHs) as an anion adsorbent could be applied into this area owing to their good capacity on P-adsorption and low cost on preparation. Interestingly, SIA contains more aluminum (Al) and iron (Fe) needed to be removed prior to P-recovery, and removed Al and Fe could be utilized to synthesize LdHs, like Mg/Al-LdH and Mg/Fe-LdH. With this study, Mg/Al-LdH-r and Mg/Fe-LdH-r were economically synthesized with Al and Fe removed from SIA, which were similar in their chemical structures to commercial LdHs. The synthesized LdHs had a high P-adsorption capacity, up to 95.0%. "NaOH + desalinated brine" as a new desorption solution could achieve a desorption ratio at about 80%, which could reduce the liquid-solid ratio by at least 60%, greatly decreasing the desorption cost. Pot trials demonstrated that the desorbed and precipitated Ca-P could promote the growth of maize as well as a commercial P-fertilizer. Furthermore, the adsorbed phosphate by LdHs could be directly used as a slow-released P-fertilizer and also improve the pH value of acidic soil, completely deleting the desorption process. © 2023, The Authors. All rights reserved.

关键词： Adsorption

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Efficient Extraction and Formation Mechanism of Fulvic Acid from Lignite: Experimental and dft Studies

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SSrN 2024年

作者： Li, Na Ma, Hang Wang, Guodong Ma, Xunmeng deng, Jin Shenfu, Yuan School of Chemical Science and Engineering Key Laboratory of Medicinal Chemistry for Natural Resource-Ministry of Education National Demonstration Center for Experimental Chemistry and Chemical Engineering Education Yunnan Key Laboratory of Carbon Neutrality and Green Low-Carbon Technologies Yunnan University 2 North Cuihu Road Yunnan Kunming 650091 China R&D Center Yunnan Yuntianhua CO. LTD NO.1417 Dian Lake Road Kunming650228 China

As a kind of fossil energy, coal plays an important role in human daily life. Coal-based fulvic acid (FA) was used widely in many fields such as modern agriculture, ecological restoration and life sciences because of its unique chemical properties. However, the efficient and environmentally friendly preparation of FA and its formation mechanism still need to be realized. In this study, ML lignite and JS lignite were used as raw materials to prepare coal-based FA by H2O2 and NaOH/KOH oxidation. By exploring extraction temperature, time, NaOH/KOH concentration and other conditions, the optimal process conditions were selected. Meanwhile, HCl was used to acid-washed ML lignite. After acid-washed, the coal-based FA yield could reach 60.49%. Under the action of •O2- and •OH radicals, the carboxyl group was prone to delocalization reaction. Adding silicone defoamer can effectively prevent excessive foam during the reaction and inhibit the excessive oxidation of -COOH to CO2, thereby increasing the yield of FA. The composition and microstructure of coal-based FA were analyzed through characterization. The characterization results showed that because FA has more polar functional groups (-COOH, -OH), the solubility of FA increases, thereby increasing the yield of FA. Moreover, the Gibbs free energies of •O2-, HO2• and •OH radicals during the formation process of coal-based FA were calculated by density-functional theory (dFT) to oxidize the bond-breaking of the macromolecular structure in lignite, which further proved the feasibility of the mechanism of coal-based FA formation. © 2024, The Authors. All rights reserved.

关键词： density functional theory

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rAPId ANTIBIOTIC SUSCEPTIBILITY TESTING OF BACTErIA BY SINGLE-FIELd TrACKING CENTrIFUGATION OF BACTErIA SOLUTION 12

RAPID ANTIBIOTIC SUSCEPTIBILITY TESTING OF BACTERIA BY SINGL...

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Optics in Health Care and Biomedical Optics XII 2022

作者： Zhang, Xiaonan Wang, Xuenian Shen, Zhiyuan Xu, Yang Wang, Bei Zhang, Haowei Guan, Tian He, Yonghong School of Medicine Tsinghua University Beijing100084 China Institute of Optical Imaging and Sensing Shenzhen Key Laboratory for Minimal Invasive Medical Technologies Shenzhen International Graduate School Tsinghua University Shenzhen518055 China Medical Optical Technology R&D Center Research Institute of Tsinghua Pearl River Delta Guangzhou510700 China School of Life Sciences Tsinghua University Beijing100084 China Department of Physics Tsinghua University Beijing100084 China

ISBN: (纸本)9781510657069

The misuse of antibiotics has led to the emergence of drug-resistant strains of bacteria, increasing the difficulty and cost of medical treatment. Antibiotic susceptibility testing(AST) of bacteria can guide doctors and other relevant personnel to issue the appropriate amount of antibiotics, and traditional AST methods cannot give timely results. Although the existing rapid AST methods can reduce the testing time to less than 4 hours, they still have problems of high complexity and high cost. In this study, we achieved rapid AST by centrifuging the bacterial solution, rapidly settling the bacteria to the bottom of the counting plate, and then tracking the growth of the bacteria in a single field of view. Our method is consistent with the results obtained by the gold standard microbial broth dilution method for AST experiments and reduces the time to 4 hours. Compared to other rapid AST methods, our method reduces cost and complexity and is suitable for clinical and point-of-care applications. © 2022 SPIE.

关键词： Escherichia coli

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UBIQUITOUS IOT WITH INTEGrATEd SPACE, AIr, GrOUNd, ANd OCEAN NETWOrKS

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IEEE NETWOrK 2021年第5期35卷 98-99页

作者： Bai, Tianyang Ben-Othman, Jalel Han, Shuai Kadoch, Michel Li, Wenjing rong, Bo Qualcomm Technol Inc Wireless R&D San Diego CA 92121 USA Univ Paris 13 Villetaneuse France CentraleSupelec L2S Lab Paris France Harbin Inst Technol Dept Elect & Commun Engn Harbin Peoples R China Univ Quebec Ecole Technol Super ETS Montreal PQ Canada BUPT Beijing Peoples R China Key Lab Network Management Res Ctr Beijing Peoples R China China Commun Stand Assoc Beijing Peoples R China First Natl Key Res & Dev Program China 6G Network Beijing Peoples R China Commun Res Ctr Ottawa ON Canada

Global connections to everyone and anything are becoming increasingly important as Internet of Things (IoT) applications and services spread throughout our society. Such needs cannot be met by terrestrial networks alone. Hence the idea of combining space, air, ground, and water communication infrastructures has emerged. The integrated platform that emerges from this process should not simply be a mashup of several networks and domains. It is extremely difficult to manage heterogeneity and hybridity's full potential at the world's largest geographical scale ever.

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Architecture design of IoT cloud platform for industrial monitoring

Architecture design of IoT cloud platform for industrial mon...

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Computer, Internet of Things and Control Engineering (CITCE), International Conference on

作者： Maoduo Li Beijing Key Laboratory of Big Data in Security & Protection Industry Communication University of China National Key R&D Program of China(2020YFF0305300) Beijing China

ISBN: (纸本)9781665421850

In order to achieve the purpose of remote monitoring and control, the combination of the Internet of Things and cloud computing is an inevitable trend, but the combination of the two will also bring challenges in network communication, security and data management. In response to these challenges, this paper proposes an industrial IoT cloud platform architecture. The platform includes four main modules: MQTT Broker, certification center, data management, and web application. The design of the modules is analyzed, and finally the platform functions are tested. The results show that the design of these modules has achieved the expected effect.

关键词： Cloud computing Protocols databases Memory Computer architecture Production Market research

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Synthesis of Size-Controllable NiCo2S4 Hollow Nanospheres Toward Enhanced Electrochemical Performance

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Energy & Environmental Materials 2020年第3期3卷 421-428页

作者： Lei Zhang Haitao Zhang Xiang Chu Fangyan Liu Chunsheng Guo Weiqing Yang Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China School of Physic Science&Technology Superconductivity and New Energy R&D CenterSouthwest Jiaotong UniversityChengdu Sichuan 610031China State Key Laboratory of Traction Power Southwest Jiaotong UniversityChengdu 610031China

Although the synthesis of novel nanostructured metal sulfides has been well established,further size-controllable optimization is still valuable to enhance their performance for various ***,a self-template method to size-controllably synthesize the hollow NiCo2S4 nanospheres is *** monodisperse Ni Co precursors with diameter widely ranging from 97 to 550 nm are controllably synthesized and subsequently transformed into hollow NiCo2S4 nanospheres through in situ *** nanoparticles’diameter results in the hollow NiCo2S4 nanospheres larger surface area and thinner shell thickness and hence provides much more electrochemical active sites as well as facilitate the ion and electron ***,the hollow NiCo2S4 nanospheres—used as the electrode materials in supercapacitors—achieve 19%enhancement of specific capacity from 484.8 to 575.1 C g-1 through lowering the 42.5%diameter of hollow NiCo2S4 nanospheres from 407 to 234 ***,the hollow NiCo2S4 nanospheres with 234 nm diameter exhibit superior rate capacity indicated by 49%capacity retention from 1 to 50 A g-1 and excellent cycling stability(77%after 2000 cycles).Furthermore,this method is a potentially general strategy in the size-controllable synthesis of the metal sulfides hollow nanostructures and results in the remarkable electrochemical applications.

关键词： nickel-cobalt sulfides self-template method size-controllable supercapacitor

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High density Fe-Based Soft Magnetic Composites with Excellent Magnetic Properties Prepared by Warm Compaction

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SSrN 2023年

作者： Huang, Haohui Zhang, rui Sun, Haibo Zhang, Jichao Wang, Jinghui School of Materials Science and Hydrogen Energy Foshan University Foshan528000 China Guangdong Key Laboratory for Hydrogen Energy Technologies Foshan University Foshan528000 China Product R&D Center Foshan Zhongyan Magnetoelectric Technology Co. LTD. Foshan528241 China Intelligent Manufacturing Institute Foshan Polytechnic Foshan528137 China

The influences of compacting temperature (Tc), molding pressure (Pm), contents of lubricant (Cl) and binder (Cb) on the compacted density (ρc) and its comprehensive soft magnetic properties for the FeSiCr soft magnetic composites (SMCs) fabricated by warm compaction were systematically studied. Appropriate increase of Tc is beneficial to improve the surface lubrication effect of magnetic powders during the forming process of SMCs, thus giving rise to the increase of ρc and the decrease of porosity fraction for the SMCs. Under this situation, the resistance of domain rotation and domain wall displacement in the SMCs during magnetization process is reduced, which is finally in favor of the improvement of SMCs’s soft magnetic properties. The results of loss separation show that the Ph value plays a dominant role in the variation trend of Pcv value for the FeSiCr-SMCs in the test frequency range of 20~200 KHz, wherein the ratio of Ph/Pcv is large than 81%. Under the optimized process parameters of Tc=110℃, Pm=600MPa, Cl=1.5wt% and Cb=2.5wt%, the ρc of FeSiCr-SMCs increases by 8.14% to 6.38 kg/m3, and the SMCs exhibit better comprehensive soft magnetic properties compared with those at Tc=25℃. Hereinto, μe increases by 19.89% to 45.8@100kHz, Pcv decreases by 26.11% to 495.8kW/m3@f=100kHz, Bm=50mT, and μe% reached 85.5% at H=8000A/m. These results indicated that the warm compaction process is one of the effective ways to further promote Fe-based SMCs to meet the development requirements of high-frequency, miniaturization and high-power of electronic equipment. © 2023, The Authors. All rights reserved.

关键词： Compaction

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