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Corrigendum to "Uptake and translocation of UV-filters and synthetic musk compounds into edible parts of tomato grown in amended soils" [Sci. Total Environ. 792 (2021) 148482]

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The Science of the total environment 2022年 808卷 152068页

作者： Sara Ramos Pedro Humberto Castro Vera Homem Lúcia Santos LEPABE - Laboratory for Process Engineering Environment Biotechnology and Energy Faculty of Engineering University of Porto Rua Dr. Roberto Frias 4200-465 Porto Portugal. CIBIO Centro de Investigação em Biodiversidade e Recursos Genéticos InBIO Laboratório Associado Campus de Vairão Universidade do Porto 4485-661 Vairão Portugal BIOPOLIS Program in Genomics Biodiversity and Land Planning CIBIO Campus de Vairão 4485-661 Vairão Portugal. LEPABE - Laboratory for Process Engineering Environment Biotechnology and Energy Faculty of Engineering University of Porto Rua Dr. Roberto Frias 4200-465 Porto Portugal. Electronic address: vhomem@fe.up.pt.

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Biodegradability of Partially Reduced Nanographene Oxide by Human, Plant and Microbial Enzymes: Impact of Magnetic Nanoparticles

SSRN

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SSRN 2024年

作者： Kurapati, Rajendra Swetha, K. Sudeshna, Samantaray Silva, Filipa A.L.S. Silva, Filipa C. Freitas, Bruno Incorvia, Jean Anne C. Banerjee, Sourav Afonso Fernandes, José Ramiro Jayaraj, Arya Singh, N. Sadananda Magalhães, Fernão D. Pinto, Artur M. School of Chemistry Indian Institute of Science Education and Research Thiruvananthapuram Maruthimala PO Thiruvananthapuram India LEPABE - Laboratory for Process Engineering Environment Biotechnology and Energy Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias s/n Porto4200-180 Portugal ALiCE - Associate Laboratory in Chemical Engineering Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias s/n Porto4200-180 Portugal i3S - Instituto de Investigação e Inovação em Saúde Universidade do Porto Rua Alfredo Allen 208 Porto4200-180 Portugal INEB - Instituto de Engenharia Biomédica Universidade do Porto Rua Alfredo Allen 208 Porto4200-180 Portugal IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto Universidade do Porto Porto4200-180 Portugal Department of Electrical and Computer Engineering University of Texas at Austin. AustinTX78712 United States CQVR – Centro de Química Vila Real Universidade de Trás-os-Montes e Alto Douro Portugal Physical Department University of Trás-os-Montes and Alto Douro Quinta dos Prados Vila Real5000-801 Portugal School of Biology Indian Institute of Science Education and Research Thiruvananthapuram Maruthimala PO Thiruvananthapuram India

Although graphene-based materials continuously expand their range of industrial and biomedical applications, understanding their long-term fate in the organisms and environment is still in its infancy. Herein we examine the biodegradation of partially reduced graphene oxide (p-rGOn) and magnetic nanoparticles (IONPs) embedded rGOn (@rGOn) by incubating with the human myeloperoxidase (hMPO) isolated from the neutrophils, including with plant enzyme, horseradish peroxidase and treating with microbial laccase in the presence of its redox mediator ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]. Further, the biodegradation of p-rGOn and @rGOn was investigated by incubating with the laccase-secreting fungi, Aspergillus sp, present in the compost soil. The biodegradation of both rGO samples was analyzed for morphological and structural changes using electron microscopy and Raman spectroscopy. The results confirmed the efficient degradation of p-rGOn and that the presence of IONPs enhanced the biodegradability of @rGOn (magnetic) over p-rGOn, which could be attributed to the Fenton-type reaction because of IONPs. These results confirmed that the presence of IONPs facilitates the magnetic manipulation of @rGOn and increases their biodegradability. The current work could be crucial for better designing the potential biomedical applications of @rGOn (magnetic) for near-infrared photothermal (PTT) and externally controlled magnetotherapy applications useful in cancer treatment. © 2024, The Authors. All rights reserved.

关键词： Enzymes

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Application of magnetic nanoparticles for water purification

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Environmental Advances 2020年 2卷

作者： Pinto, Mariana Ramalho, P.S.F. Moreira, N.F.F. Gonçalves, A.G. Nunes, O.C. Pereira, M.F.R. Soares, O.S.G.P. Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials LSRE-LCM Departamento de Engenharia Química Faculdade de Engenharia Universidade do Porto Porto 4200-465 Portugal Laboratory for Process Engineering Environment Biotechnology and Energy LEPABE Departamento de Engenharia Química Faculdade de Engenharia da Universidade do Porto Porto 4200-465 Portugal Bosch Termotecnologia Estrada Nacional 16 Cacia 3800-533 Aveiro Portugal

Water is one of the main sources of human pathogenic microorganisms in developing countries. Therefore, new low-cost water treatment technologies are required to prevent public health problems. The main goal of this work was to develop a water purification technology using magnetic nanoparticles, to be applied in a water outlet point where the contact time between the two phases is minimal. For that, six different nanomaterials were synthesised based on iron oxide (FeO): FeO, mixed oxides of Fe with Mn, Co or Cu, a composite of FeO with activated carbon, and FeO subsequently coated with carbon by chemical vapour deposition (CVD). Different techniques were used to characterise these nanomaterials and their ability to remove E. coli (a Gram-negative bacteria) and also S. aureus (a Gram-positive bacteria) cells from a suspension was assessed. CuFeO, FeO, MnFeO and FeO/AC composite MNPs showed high removal efficiencies of Escherichia coli (Gram-negative). For the nanoparticles with higher removal efficiencies in the first tests (FeO and CuFeO), 50 mg mL⁻¹ was the optimal concentration of particles and 1 min of contact time it was enough to obtain high removal efficiencies. The removal efficiency of S. aureus was higher than that of E. coli when FeO particles were used. In contrast, the removal efficiency of E. coli was higher than that of S. aureus for CuFeO particles. For both particles, the removal efficiency of microorganisms for the well water sample was lower when compared with the bacteria suspensions, being 64.1 % for FeO and 91.8 % for CuFeO. The reuse tests showed that these particles could be re-used several times without losing efficiency in bacteria removal. The MNPs used are simple and low-cost and show promising results for the elimination of both types of bacteria (gram-positive and gram-negative) demonstrating that this technology is a promising alternative to the conventionally used processes showing an easy, efficient, and inexpensive method

关键词： Bacteria removal Drinking water Magnetic nanoparticles (MNPs) Water purification

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Occurrence and diversity of Listeria monocytogenes in Portuguese dairy farms

Microbe (Netherlands)

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Microbe (Netherlands) 2024年 3卷

作者： Nunes, Bárbara Barata, Ana Rita Oliveira, Ricardo Guedes, Hugo Almeida, Carina da Silva, Gabriela Jorge Nogueira, Teresa Saavedra, Maria José Almeida, Gonçalo National Institute for Agricultural and Veterinary Research (INIAV) I.P Vila do Conde Portugal Veterinary Sciences Department Antimicrobials Biocides & Biofilms Unit University of Trás-os-Montes and Alto Douro (UTAD) Vila Real Portugal School of Life Science and Environment Escola de Ciências da Vida e do Ambiente Universidade de Trás-os-Montes e Alto Douro University of Trás-os-Montes and Alto Douro (UTAD) Vila Real Portugal Laboratory for Process Engineering Environment Biotechnology and Energy (LEPABE) Porto Portugal Centre of Biological Engineering (CEB) University of Minho Braga Portugal Faculty of Pharmacy and Center for Neurosciences and Cell Biology University of Coimbra Coimbra Portugal Centre for Ecology Evolution and Environmental Changes (cE3c) & Global Change and Sustainability Institute (CHANGE) Faculdade de Ciências da Universidade de Lisboa Lisboa Portugal Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB) and Institute for Innovation Capacity building and Sustainability of Agri-Food production (Inov4Agro) University of Trás-os-Montes e Alto Douro Vila Real Portugal Centre for Study in Animal Science (CECA) ICETA University of Porto Porto Portugal

Listeria monocytogenes is a pathogenic microorganism that causes listeriosis, an infection that usually occurs after consumption of contaminated food and is considered particularly dangerous due to its ability to grow and multiply under adverse conditions. In recent years, there has been an increase in the consumption of unprocessed products, such as raw milk and dairy products, by people of all ages, including those with compromised immune systems, which could lead to an increase in foodborne illness. Ruminants play a very important role in the persistence and transmission of L. monocytogenes through a continuous oral-faecal cycle. Therefore, farms are considered a reservoir of this microorganism and are involved in the transmission from animals to humans. In this study, samples of faeces, milk, water, silage, feed and teat cups swabs were collected from 8 farms to assess the distribution of the pathogen in the farm environment. Milk samples were also collected from 100 dairy farms to assess the risk associated with the consumption of raw milk. Detection was performed by real-time PCR, while preparation, enrichment and confirmation were performed according to ISO 11290–1, (2017). The prevalence in water was 8.3%, in faeces 12.5% and in feed 12.0%, while in the other samples the microorganism was not detected. It was also observed that this microorganism was more abundant in spring months. The eight isolates were serotyped by real-time PCR and the most frequent serogroup was IVb with 5 isolates (2 of which were IVb-v1) and the remaining 3 were IIb. Two of the clonal complexes (CCs) identified were shared by two isolates (CC 213 and CC 217), the remaining CCs identified (CC 392, CC 554, CC 489, CC 224 and CC 183) were not identified in more than one isolate. This study contributed to a better understanding of the ecology of L. monocytogenes in dairy farms, showing that most of the clones found in food were not present in this environment and that genes coding for dis

关键词： Cow's milk Dairy Food safety Foodborne pathogens Listeria WGS

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Corrigendum to Minimizing mass-transport loss in proton exchange membrane fuel cell by freeze-drying of cathode catalyst layers [J. Power Sour. Vol. 427, 1 July 2019, Pages 309–317]

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Journal of Power Sources 2020年 465卷

作者： Krishan Talukdar Sofia Delgado Tiago Lagarteira Pawel Gazdzicki K. Andreas Friedrich German Aerospace Center (DLR) Institute of Engineering Thermodynamics Pfaffenwaldring 38-40 Stuttgart 70569 Germany Laboratory for Process Engineering Environmental Biotechnology and Energy (LEPABE) Faculty of Engineering of University of Porto Rua Roberto Frias S/n 4200-465 Porto Portugal University of Stuttgart Institute of Building Energetics Thermal Engineering and Energy Storage (IGTE) Pfaffenwaldring 31 70569 Stuttgart Germany

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Correction: Syngas production by bi-reforming methane on an Ni-K-promoted catalyst using hydrotalcites and filamentous carbon as a support material

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RSC advances 2020年第41期10卷 24492页

作者： Adelino F Cunha Sergio Morales-Torres Luisa M Pastrana-Martínez António A Martins Teresa M Mata Nídia S Caetano José M Loureiro Laboratory of Separation and Reaction Engineering Associate Laboratory LSRE-LCM Department of Chemical Engineering Faculty of Engineering University of Porto Rua Dr. Roberto Frias 4200-465 Porto Portugal. School of Engineering (ISEP) Polytechnic of Porto (P.Porto) R. Dr. António Bernardino de Almeida 431 4249-015 Porto Portugal afccu@isep.ipp.pt +351 22 83 21 159 +351 22 83 41 904. Carbon Materials Research Group Department of Inorganic Chemistry Faculty of Sciences University of Granada Avenida de Fuentenueva s/n 18071 Granada Spain semoto@ugr.es +34 958248526 +34 958240443. LEPABE - Laboratory for Process Engineering Environment Biotechnology and Energy Faculty of Engineering University of Porto Rua Dr. Roberto Frias 4200-465 Porto Portugal.

[This corrects the article DOI: 10.1039/D0RA03264F.].

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Protein crystallization in a novel meso oscillatory flow reactor 22

Protein crystallization in a novel meso oscillatory flow rea...

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22nd International Congress of Chemical and process engineering, CHISA 2016 and 19th Conference on process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction, PRES 2016

作者： Castro, F. Ferreira, A. Teixeira, J.A. Rocha, F. LEPABE-Laboratory for Process Engineering Environment Biotechnology and Energy Faculty of Engineering of Porto Dep. of Chemical Engineering Univ. of Porto Rua Roberto Frias s/n Porto4200-465 Portugal Centre of Biological Engineering Dep. of Biological Engineering Univ. of Minho Campus de Gualtar Braga4710-057 Portugal

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#126. Nova estratégia para detetar e localizar patogénicos periodontais: a técnica de PNA‐FISH

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Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial 2016年 57卷 52-52页

作者： Luzia Mendes Rui Rocha Andreia S. Azevedo Mariana Henriques Miguel G. Pinto Nuno F. Azevedo Faculdade de Medicina Dentária da Universidade do Porto LEPABE – Laboratory for Process Engineering Environment Biotechnology and Energy FEUP LIBRO – Laboratório de Investigação em Biofilmes Rosário Oliveira Universidade do Minho

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Removal of paraquat pesticide with Fenton reaction in a pilot scale water system

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Drinking Water engineering and Science 2014年第1期7卷 11-21页

作者： Oliveira, C. Gruskevica, K. Juhna, T. Tihomirova, K. Alves, A. Madeira, L.M. LEPABE - Laboratory for Process Engineering Environment Biotechnology and Energy Department of Chemical Engineering University of Porto R. Dr. Roberto Frias s/n 4200-465 Porto Portugal Department of Water Engineering and Technology Riga Technical University 16 Azenes street Riga Latvia

Advanced oxidation processes, such as the Fenton's reagent, are powerful methods for decontamination of different environments from recalcitrant organics. In this work, the degradation of paraquat (PQ) pesticide was assessed (employing the commercial product gramoxone) directly inside the pipes of a pilot scale loop system;the effect of corroded cast iron pipe and loose deposits for catalysing the process was also evaluated. Results showed that complete degradation of paraquat ([PQ]0Combining double low line 3.9 × 10−4 M,TCombining double low line 20-30 C, pH0 Combining double low line 3, [H2O2]0 Combining double low line 1.5 × 10-2 M and [Fe (II)] Combining double low line 5.0 × 10−4 M,) was achieved within 8 h, either in lab scale or in the pilot loop. Complete PQ degradation was obtained at pH 3 whereas only 30% of PQ was degraded at pH 5 during 24 h. The installation of old cast iron segments with length from 0.5 to 14 m into PVC pipe loop system had a significant positive effect on degradation rate of PQ, even without addition of iron salt;the longer the iron pipes section, the faster was the pesticide degradation. Addition of loose deposits (mostly corrosion products composed of goethite, magnetite and a hydrated phase of FeO) also catalysed the Fenton reaction due to presence of iron in the deposits. Moreover, gradual addition of hydrogen peroxide improved gramoxone degradation and mineralization. This study showed for the first time that is possible to achieve complete degradation of pesticides in situ pipe water system and that deposits and corroded pipes catalyse oxidation of pesticides. © Author(s) 2014.

关键词： Polyvinyl chlorides

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Erratum to: mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids

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Applied microbiology and biotechnology 2015年第9期99卷 4117页

作者： Silvia Fontenete Joana Barros Pedro Madureira Céu Figueiredo Jesper Wengel Nuno Filipe Azevedo LEPABE-Laboratory for Process Engineering Environment Biotechnology and Energy Faculty of Engineering University of Porto Rua Dr. Roberto Frias 4200-465 Porto Portugal.

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