Ovarian cancer(OC),a gynecologic cancer,has a poor prognosis and limited treatment *** than 75％of patients are initially diagnosed with advanced OC[1].The 5-year overall survival rate of OC has remained virtually unc...
Ovarian cancer(OC),a gynecologic cancer,has a poor prognosis and limited treatment *** than 75％of patients are initially diagnosed with advanced OC[1].The 5-year overall survival rate of OC has remained virtually unchanged,which means it is still low[2].Therefore,research on OC is unceasing,and new therapeutic targets and biomarkers are urgently needed[3].
Objective Bacillus strains are well known for their natural bioactive products that have antimicrobial and/or anti-cancer *** of Bacillus’structurally unique metabolites can combat human diseases,including ***,becaus...
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Objective Bacillus strains are well known for their natural bioactive products that have antimicrobial and/or anti-cancer *** of Bacillus’structurally unique metabolites can combat human diseases,including ***,because Bacillus’metabolites are so abundant,few have been studied extensively enough to fully characterize their chemical constitutions and biological *** In this study,we focused on the isolation and purification of a new Bacillus strain,and determined the effects of its metabolites on bacteria and cancer *** study focused on a new strain of Bacillus isolated from deer *** on BLAST results,this isolate belongs to Bacillus subtilis,and therefore we named the strain Bacillus subtilis *** red assay was used to test the cellulase *** inhibition zone was measured to test the antimicrobial ***-8,wound healing and flow cytometry were used to test the anti-cancer *** Metabolites from Bacillus subtilis NC16 have both antimicrobial and anti-cancer *** can both suppress the growth of Trichoderma vride and Staphylococcus aureus,and inhibit the proliferation and promote the apoptosis of non-small cell lung cancer cell *** Our results suggest that Bacillus subtilis NC16 can not only degrade cellulose,but its metabolites may be sources of antibiotics and anti-cancer drugs.
*** Wang(王德宝)was bom on May 7th,1918,in Taixing,Jiangsu Province,*** the age of 18,he was admitted to the Agricultural Chemistry Department of the National Central University,and graduated in *** the summer of 1943...
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*** Wang(王德宝)was bom on May 7th,1918,in Taixing,Jiangsu Province,*** the age of 18,he was admitted to the Agricultural Chemistry Department of the National Central University,and graduated in *** the summer of 1943,*** worked in the Department of Biochemistry,School of Biological Medicine,under the supervision of *** ***(Zhang,2020).At that time,he had a dream to contribute to the development of his motherland,which inspired him to study aboard.
Objective: Positive peritoneal lavege cytology(CY1) gastric cancer is featured by dismal prognosis, with high risks of peritoneal metastasis. However, there is a lack of evidence on pathogenic mechanism and signature ...
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Objective: Positive peritoneal lavege cytology(CY1) gastric cancer is featured by dismal prognosis, with high risks of peritoneal metastasis. However, there is a lack of evidence on pathogenic mechanism and signature of CY1and there is a continuous debate on CY1 therapy. Therefore, exploring the mechanism of CY1 is crucial for treatment strategies and targets for CY1 gastric ***: In order to figure out specific driver genes and marker genes of CY1 gastric cancer, and ultimately offer clues for potential marker and risk assessment of CY1, 17 cytology-positive gastric cancer patients and 31matched cytology-negative gastric cancer patients were enrolled in this study. The enrollment criteria were based on the results of diagnostic laparoscopy staging and cytology inspection of exfoliated cells. Whole exome sequencing was then performed on tumor samples to evaluate genomic characterization of cytology-positive gastric ***: Least absolute shrinkage and selection operator(LASSO) algorithm identified 43 cytology-positive marker genes, while Mut Sig CV identified 42 cytology-positive specific driver genes. CD3G and CDKL2 were both driver and marker genes of CY1. Regarding mutational signatures, driver gene mutation and tumor subclone architecture, no significant differences were observed between CY1 and negative peritoneal lavege cytology(CY0).Conclusions: There might not be distinct differences between CY1 and CY0, and CY1 might represent the progression of CY0 gastric cancer rather than constituting an independent subtype. This genomic analysis will thus provide key molecular insights into CY1, which may have a direct effect on treatment recommendations for CY1and CY0 patients, and provides opportunities for genome-guided clinical trials and drug development.
The 5-year survival rate of lung cancer is one of the lowest among various malignant *** noncodingrnas (lncrnas),noncodingrnas longer than 200 nucleotides,can function either as tumor suppressors or as *** aim of th...
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The 5-year survival rate of lung cancer is one of the lowest among various malignant *** noncodingrnas (lncrnas),noncodingrnas longer than 200 nucleotides,can function either as tumor suppressors or as *** aim of this study is to investigate the function of lncrna LINC01296 and its molecular mechanism in non-small-cell lung cancer (NSCLC).Accord-ing to the Gene Expression Omnibus database,10 differentially expressed lncrnas in NSCLC cells and patient tissues are ***01296 is the one with the most significant *** of LINC01296 inhibits the growth and migration,arrests the cell cycle,and promotes the apoptosis of NSCLC *** down LINC01296 in vivo suppresses tumor growth and ***01296 also acts as the sponge of *** the expres-sion of LINC01296 leads to decreased expression of autophagy-related 2B (ATG2B),a target gene of ***,downregulation of LINC01296 promotes paclitaxel sensitivity in *** results demonstrated that the LINC01296/miR-143-3p/ATG2B axis is crucial in promoting the development of NSCLC and paclitaxel *** study may provide new ideas for the further research of clinical chemotherapy of NSCLC in the near future.
BCL-2 gene as well as its products is recognized as a promising target for the molecular targeted therapy of ***,due to certain defense measures of tumor cells,the therapeutic effect based on the gene silencing of BCL...
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BCL-2 gene as well as its products is recognized as a promising target for the molecular targeted therapy of ***,due to certain defense measures of tumor cells,the therapeutic effect based on the gene silencing of BCL-2 is greatly *** we fabricate a smart response nucleic acid therapeutic that could silence the gene effectively through a dual-targeted and cascade-enhanced *** brief,nano-graphene oxide(GO),working as a nano-carrier,is loaded with a well-designed DNAzyme,which can target and silence the BCL-2 ***,upon binding with the BCL-2 mrna,the enzymatic activity of the DNAzyme can be initiated,cutting a substrate oligonucleotide to produce an anti-nucleolin aptamer ***,a nucleolar phosphoprotein,is known as a stabilizer of BCL-2 *** binding and inactivating the nucleolin,AS1411 can destabilize BCL-2 *** this means of simultaneously targeting mrna and its stabilizer in an integrated system,effective silencing of the BCL-2 gene of tumor cells is achieved at both the cellular and in vivo *** being dosed with this nucleic acid therapeutic and without any chemotherapeutics,apoptosis of tumor cells at the cellular level and apparent shrinkage of tumors in vivo are *** labeling a molecular beacon on the substrate of DNAzyme,visualization of the enzymatic activity as well as the tumor in vivo can be also *** work presents a pure bio-therapeutic strategy that has positive implications for enhancing tumor treatment and avoiding side effects of chemotherapeutics.
Mitophagy is a degradative pathway that mediates the degradation of the entire mitochondria,and defects in this process are implicated in many diseases including *** mammals,mitophagy is mediated by BNIP3L (also known...
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Mitophagy is a degradative pathway that mediates the degradation of the entire mitochondria,and defects in this process are implicated in many diseases including *** mammals,mitophagy is mediated by BNIP3L (also known as NIX) that is a dual regulator of mitochondrial turnover and programmed cell death *** myeloid leukemia (AML) cells with deficiency of BNIP3L are more sensitive to mitochondria-targeting *** small molecular inhibitors for BNIP3L are currently not *** immunomodulatory drugs (IMiDs) have been proved by FDA for hematologic malignancies,however,the underlining molecular mechanisms are still elusive,which hindered the applications of BNIP3L inhibition for AML *** this study we carried out MS-based quantitative proteomics analysis to identify the potential neosubstrates of a novel thalidomide derivative CC-885 in A549 *** total,we quantified 5029 proteins with 36 downregulated in CRBN+/+ cell after CC-885 *** analysis showed that macromitophagy pathway was enriched in the negative pathway after CC-885 *** further found that CC-885 caused both dose-and time-dependent degradation of BNIP3L in CRBN+/+,but not CRBN-/-***,our data uncover a novel role of CC-885 in the regulation of mitophagy by targeting BNIP3L for CRL4CRBN E3 ligase-dependent ubiquitination and degradation,suggesting that CC-885 could be used as a selective BNIP3L degradator for the further ***,we demonstrated that CC-885 could enhance AML cell sensitivity to the mitochondria-targeting drug rotenone,suggesting that combining CC-885 and mitochondria-targeting drugs may be a therapeutic strategy for AML patients.
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