Background:Single-nucleotide polymorphisms(SNPs)-associated genes and long non-coding rnas(lncrnas)can contribute to human *** comprehensively investigate the contribution of lncrnas to breast cancer,we performed the ...
详细信息
Background:Single-nucleotide polymorphisms(SNPs)-associated genes and long non-coding rnas(lncrnas)can contribute to human *** comprehensively investigate the contribution of lncrnas to breast cancer,we performed the first genome-wide lncrna association study on Han Chinese ***:We designed an lncrna array containing>800,000 SNPs,which was incorporated into a 96-array plate by Affymetrix(CapitalBio Technology,China).Subsequently,we performed a two-stage genome-wide lncrna association study on Han Chinese women covering 11,942 individuals(5634 breast cancer patients and 6308 healthy controls).Additionally,in vitro gain or loss of function strategies were performed to clarify the function of a novel SNP-associated ***:We identified a novel breast cancer-associated susceptibility SNP,rs11066150(P_(meta)=2.34×10^(-8)),and a previously reported SNP,rs9397435(P_(meta)=4.32×10^(-38)),in Han Chinese ***11066150 is located in NONHSAT164009.1(lncHSAT164),which is highly expressed in breast cancer tissues and cell ***164 overexpression promoted colony formation,whereas lncHSAT164 knockdown promoted cell apoptosis and reduced colony formation by regulating the cell ***:Based on our lncrna array,we identified a novel breast cancer-associated lncrna and found that lncHSAT164 may contribute to breast cancer by regulating the cell *** findings suggest a potential therapeutic target in breast cancer.
The pirna machinery is known for its role in mediating epigenetic silencing of transposons. Recent studies suggest that this function also involves pirna-guided cleavage of transposon-derived transcripts. As many piRN...
详细信息
The pirna machinery is known for its role in mediating epigenetic silencing of transposons. Recent studies suggest that this function also involves pirna-guided cleavage of transposon-derived transcripts. As many pirnas also appear to have the capacity to target diverse mrnas, this raises the intriguing possibility that pirnas may act extensively as sirnas to degrade specific mrnas. To directly test this hypothesis, we compared mouse PIWI (MIWI)-associated pirnas with experimentally identified cleaved mrna fragments from mouse testes, and observed cleavage sites that predominantly occur at position 10 from the 5′ end of putative targeting pirnas. We also noted strong biases for U and A residues at nucleotide positions 1 and 10, respectively, in both pirnas and mrna fragments, features that resemble the pattern of pirna amplification by the 'ping-pong' cycle. Through mapping of MIWI-rna interactions by CLIP-seq and gene expression profiling, we found that many potential pirna-targeted mrnas directly interact with MIWI and show elevated expression levels in the testes of Miwi catalytic mutant mice. Reporter-based assays further revealed the importance of base pairing between pirnas and mrna targets and the requirement for both the slicer activity and pirna-loading ability of MIWI in pirna-mediated target repression. Importantly, we demonstrated that proper turnover of certain key pirna targets is essential for sperm formation. Together, these findings reveal the sirna-like function of the pirna machinery in mouse testes and its central requirement for male germ cell development and maturation.
Intermediate-size(50-500nt)non-coding rnas(is-ncrnas)have been recognized as important players during the development of several eukaryotic *** parasitic protozoa Plasmodium falciparum is the most deadly Plasmodium sp...
详细信息
Intermediate-size(50-500nt)non-coding rnas(is-ncrnas)have been recognized as important players during the development of several eukaryotic *** parasitic protozoa Plasmodium falciparum is the most deadly Plasmodium species that cause human *** identification of ncrnas in *** has been conducted in several studies,the whole profile of is-ncrnas is still largely not
暂无评论