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18th Chinese Conference on Image and Graphics technology and Application Conference, IGTA 2023

作者： Qian, Jing Kong, Bin Yang, Jing Wang, Can Hefei Institutes of Physical Science Chinese Academy of Sciences Hefei230031 China University of Science and Technology of China Hefei230026 China Peng Cheng Laboratory Shenzhen518053 China Anhui Key Laboratory of Biomimetic Sensing and Advanced Robot Technology Hefei230031 China School of Artificial Intelligence and Big Data Hefei University Hefei230031 China

ISBN: (纸本)9789819975488

Contrast is one of the commonly used indicators for evaluating image quality and is related to color information. However, the traditional contrast calculation method only reflects the clarity of the image by counting the distribution probability of the gray difference between adjacent pixels in the region, which lacks consideration of the chrominance information. We propose a contrast calculation method considering the perceptual color difference in color images. First, we obtain each pixel’s hue and saturation information from the HSV color space. And the luminance information of each pixel is obtained while retaining the color contrast information of the original image. Then, the contrast of a color image is obtained by counting the number of adjacent pixels whose color difference exceeds the threshold and calculating the sum of color differences. Experiment results show that our method is less affected by the uniform color patches than other methods. It can effectively reflect the relationship between the contrast values of different images and reduce the information loss of color contrast. In addition, for different color images, the magnitude of the contrast value obtained by our method is consistent with the trend of human visual judgment. © 2023, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

关键词： Image quality

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Rapid prototyping of Parafilm®-based analytical microfluidic devices using laser ablation and thermal fusion bonding 26

Rapid prototyping of Parafilm®-based analytical microfluidi...

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26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

作者： Wei, Yuanyuan Wang, Tianle Wang, Yuye Ho, Yi-Ping Ho, Ho-Pui Department of Biomedical Engineering The Chinese University of Hong Kong Hong Kong Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Science Shenzhen518055 China

ISBN: (纸本)9781733419048

Motivated by the growing demand for inexpensive and versatile microfluidic chips and lab-on-a-chip (LOC) systems, we provide a rapid prototyping method for the fabrication of Parafilm®-based analytical microfluidic devices using laser ablation and thermal fusion bonding method (PLT). Laser ablation, as well as one-step bonding of multiple layers to form a 3D structure, can be realized without the necessity of photolithography and prefabricated master molds. Practical actuation devices fabricated using this PLT method including microvalves and micropumps are demonstrated. The biocompatibility of the PLT fabricated bioreactor has also been investigated. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： Microfluidics

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Fluorescence Enhancement Utilizing Dielectric Microbeads with Semi-open Microwells 16

Fluorescence Enhancement Utilizing Dielectric Microbeads wit...

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16th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2021

作者： Zhang, Pengcheng Yan, Bing Gu, Guoqiang Yu, Zitong Chen, Xi Wang, Zengbo Yang, Hui Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Science Shenzhen China

ISBN: (纸本)9781665419413

Dielectric microbeads can converge light into a narrow beam with high intensity which allows for enhancing the fluorescent signals. However, a critical challenge for the experimental realization is introducing the fluorescent samples into this extremely small beam area. Here, we design and fabricate dielectric microbeads with semi-open microwells in which localized converging light beam of high intensity can be generated. We show that fluorescent microspheres can be efficiently loaded into the semi-open microwells and thus simultaneously illuminated by the converging beam generated by the micro-optical structures without any further manipulations. Pronounced fluorescent enhancement of around 9 folds can be obtained in comparison with the fluorescent microspheres on glass substrates, stemming from the excellent convergent effect of this micro-optical structure. © 2021 IEEE.

关键词： Fluorescence

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Encapsulation of Micrornas in Exosomes for Efficient Intracellular Delivery by a Nanofluidic Platform

Encapsulation of Micrornas in Exosomes for Efficient Intrace...

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International Conference on Solid State Sensors and Actuators (TRANSDUCERS)

作者： Zitong Yu Rui Hao Sihui Chen Huitao Zhang Shi Hu Jingyi Ren Yanhang Hong Bingrun Liu Qisang Zuo Hui Yang Laboratory of Biomedical Microsystems and Nano Devices Center for Bionic Sensing and Intelligence Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences China SomesTech Co. Ltd. Shenzhen China

Here we report and validate a new strategy to package RNAs into exosomes using micro- and nanofluidic technology. We propose a nanofluidic constriction-based platform for miRNAs encapsulation into exosomes, referred to as the exosome nanoengineering platform (ExoNP). The device enables over 10 times microRNA-494 (miR494) enrichment in exosomes. When delivered intracellularly via these processed exosomes, the miR494 are biologically active in recipient cells to regulate downstream gene LRG1, further causing the repression of the proteins. Our results provide a proof-of-principle demonstration that the ExoNP can provide a straightforward and robust exogenous loading technique for packaging and intracellular delivery of therapeutic RNA molecules.

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Ultrahigh-transparency and pressure-sensitive iontronic device for tactile intelligence

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npj Flexible Electronics 2022年第1期6卷 272-285页

作者： Jie Tang Chao Zhao Qian Luo Yu Chang Zhenguo Yang Tingrui Pan Department of Materials Science Fudan UniversityShanghai 200433People’s Republic of China Bionic Sensing and Intelligence Center(BSIC) Institute of Biomedical and Health EngineeringShenzhen Institutes of Advanced TechnologyChinese Academy of ScienceShenzhenGuangdong 518055People’s Republic of China Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development ShenzhenGuangdong 518055People’s Republic of China C-MIND Center for MicroMedical Instruments and Devices University of Science and Technology of ChinaSuzhou 215123People’s Republic of China

Emerging tactile sensing devices mimic biological functions of human *** introducing the feature of optical transparency,it can lead to a combined capacities of tactile and visual intelligence into single ***,it is difficult to realize ultrahigh level of optical transparency and device sensitivity in single structure,for the widely used methods for sensitivity improvement,such as elevating the interfacial roughness,may further reduce the *** utilizing a transparent ionic material with tunable surface topologies,as well as introducing a strategy of refractive index matching,we have proposed a transparent iontronic sensing(TIS)device based on the iontronic sensing mechanism,simultaneously offering combined high device sensitivity(83.9 kPa−1),with ultrahigh optical transparency(96.9%),the highest reported value in *** from its comprehensive performance in sensing and optical characteristics,the TIS devices hold enormous potential for the humanmachine interfaces for industrial and medical applications.

关键词： transparency transparent offering

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Subwavelength Nano-imaging with a Microbottle Lens

Subwavelength Nano-imaging with a Microbottle Lens

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Asia Communications and Photonics Conference and Exhibition (ACP)

作者： Guoqiang Gu Lu Ma Pengcheng Zhang Yuye Wang Lin Zeng Hui Yang Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen China

A dielectric microbottle lens constituted by circular glass microcylinder and parabolic-shaped polymer covering layer is demonstrated here. The microbottle lens is fabricated by transferring, wetting and photocuring of UV-curable polymer on a tapered optical microfiber. By using such microbottle lens, periodic grating structures with subwavelength pitch of 360 nm can be imaged in two different imaging modes. When the pitch of the grating structures decreases to 260 nm, clear imaging of the grating structures can only be achieved in the case that the grating stripes are parallel to the microfiber axis of the microbottle lens. The results are analyzed and discussed by finite element simulation.

关键词： Silicon compounds Analytical models Shape Optical device fabrication Optical imaging Diffraction gratings Finite element analysis

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Inflection point: a perspective on photonic nanojets

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Photonics Research 2021年第7期9卷 1157-1171页

作者： GUOQIANG GU PENGCHENG ZHANG SIHUI CHEN YI ZHANG HUI YANG Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence CenterInstitute of Biomedical and Health EngineeringShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China CAS Key Laboratory of Health Informatics Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

When light propagates through the edge or middle part of a microparticle’s incoming interface,there is a basic rule that light converges and diverges rapidly or slowly at the output port. These two parts are referred to as the region of rapid change (RRC) and region of slow change (RSC),respectively. Finding the boundary point between RRC and RSC is the key to reveal and expound upon this rule scientifically. Based on the correlation between light convergence–divergence and the slope of emergent light,combined with the relationship between a natural logarithm and growth in physical reality and the second derivative of a function in practical significance,we determine the boundary point between RRC and RSC,namely,the inflection point. From such a perspective,a photonic nanojet (PNJ) and near-field focusing by light irradiation on RSC and RRC,as well as the position of the inflection point under different refractive index contrasts and the field distribution of light focusing,are studied with finite-element-method-based numerical simulation and ray-optics-based theoretical analysis. By illuminating light of different field intensity ratios to the regions divided by the inflection point,we demonstrate the generation of a photonic hook (PH) and the modulation of PNJ/PH in a new manner.

关键词： point optics slowly

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LLNet: A Lightweight Lane Line Detection Network 11th

LLNet: A Lightweight Lane Line Detection Network

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11th International Conference on Image and Graphics, ICIG 2021

作者： Zhang, Lu Kong, Bin Wang, Can Institute of Intelligent Machines Chinese Academy of Sciences Hefei230031 China University of Science and Technology of China Hefei230026 China The Key Laboratory of Biomimetic Sensing and Advanced Robot Technology Hefei230031 China

ISBN: (纸本)9783030873547

The lane line detection methods based on semantic segmentation networks have achieved remarkable results in recent years. However, the semantic segmentation networks are aimed at the pixel level. These methods have a large amount of computation, which can reduce the real-time performance. To reduce the calculation, we propose a Light-weight Lane line detection Network (LLNet). A new sub-layer is established. We also adopt a jumping structure between two sub-layers to enhance the supervisory role of ground truth. Furthermore, we adopt two branches, including instance segmentation and embeddable branch. The combination of two branches can filter out the wrongly detected pixels and further improve the accuracy of detection. Experimental results in the Tusimple dataset show that the detection accuracy of the proposed network is comparable with LaneNet. Meanwhile, it has good real-time performance, and the processing time of a single image is 10.285 ms, which is about one-half of LaneNet. © 2021, Springer Nature Switzerland AG.

关键词： Semantic Segmentation

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RNA-sequencing-based Gene Expression Profile Revealing Breast Tumor Development Induced by Exposure of Bisphenol S

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Chemical Research in Chinese Universities 2023年第3期39卷 441-448页

作者： ZHOU Peng XIAO Yu ZHOU Xin LIU Jianjun ZHAO Chao Bionic Sensing and Intelligence Center Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055P.R.China Department of Ultrasound The First Affiliated Hospital of Shenzhen University Health Science CenterShenzhen Second People’s HospitalShenzhen518035P.R.China Department of Breast and Thyroid Surgery Shenzhen Second People’s Hospital/The First Hospital Affiliated to Shenzhen UniversityShenzhen518035P.R.China Shenzhen Key Laboratory of Modern Toxicology Shenzhen Medical Key Discipline of Health Toxicology(2020—2024)Shenzhen Center for Disease Control and PreventionShenzhen518055P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China Shenzhen Key Laboratory of Precision Diagnosis and Treatment of Depression Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055P.R.China Key Laboratory of Molecular Epidemiology of Hunan Province School of MedicineHunan Normal UniversityChangsha410013P.R.China

Aberrant biological information occurs naturally at exposure to bisphenol A or its alternatives,which was associated with the occurrence and development of breast ***,the potential molecular variation in gene expression during the breast tumor development is still ***,high throughput RNA sequencing(RNA-Seq)and bioinformatics analysis were used to investigate the variation of tumor-mRNA profile exposed with BPS5(5µg/kg bw/day)or BPS50(50µg/kg bw/day)in tumor development-associated MMTV-PyMT transgenic mouse ***,we analyzed the dose-effects of bisphenol S(BPS)and BPS-induced tumor development on the gene level *** dose-effect aspects of BPS,the increased concentration of BPS significantly changed the numbers and enrichment pathway of differentially expressed genes(DEGs),especially the enrichment pathways involved in up-regulated genes including ribosome,peroxisome proliferators-activated receptor(PPAR)signaling pathway and progesterone-mediated oocyte maturation *** effects of BPS exposure to tumor development,expression of IgκC,Zfp385b,Cldn10,Pgr and Snord14d has changed significantly throughout the tumor *** ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)results obtained from BPS-induced tumor development showed that the functional classifications were intensively altered with an extension of time in high-dose BPS *** acquired DEGs and pathway information could help with the accurate exploration of molecular mechanisms of tumor development,screening of molecular targets of breast cancer,and toxicological evaluation of environmental pollutants.

关键词： Bisphenol S RNA-sequencing Breast cancer Tumor development

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Noise Reduction of UAV Using Biomimetic Propellers with Varied Morphologies Leading-edge Serration

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Journal of bionic Engineering 2020年第4期17卷 767-779页

作者： Yuliang Wei Feng Xu Shiyuan Bian Deyi Kong State Key Laboratory of Transducer Technology Key Laboratory of Biomimetic Sensing and Advanced Robot Technology of Anhui ProvinceInstitute of Intelligent MachinesChinese Academy of SciencesHefei 230031China Science Island Branch of Graduate School University of Science and Technology of ChinaHefei 230009China

The Leading-Edge(LE)serrations on owls'wings are known to be responsible for silent ***,this design has rarely been applied to reduce the noise of rotational rotor propellers and the morphologies of the existing serration designs are ***,we present a comparative study ofLE serrations with different morphologies in terms of the effectiveness in suppressing noise and promoting thrust *** performances of biomimetic propellers are investigated by Computational Fluid Dynamics(CFD)simulations and rotation *** simulation results reveal that LE serrations could reduce velocity fluctuations and change the lamina-turbulent transition and turbulence distribution on the suction surface of propeller,but the morphology of the serrations influences its *** testing results indicate that the sawtooth propeller has the best performance on noise reduction(on average 2.43 dB and in maximum 4.18 dB)and simultaneously enhancing the thrust forces(3.53%).The largest practical noise reductions(4.73 dB and 3.79 rdB)using the sawtooth propeller are observed when the quad-rotor Unmanned-Aerial Vehicle(UAV)is hovering at heights of 5 m and 8 m,*** results indicate the robustness and usefulness of owl-inspired biomimetic serration devices for aero-acoustic control and aerodynamic performance promotion on propeller *** finding is expected to con^bute to suppressing the sound of propeller and the rotor-based aircraft.

关键词： unmanned-aerial vehicle biomimetic noise reduction LE serration rotor propeller

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