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Profiling Phenotypic Heterogeneity of Circulating Tumor Cells through Spatially Resolved Immunocapture on Nanoporous Micropillar Arrays

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ACS nano 2024年第45期18卷 2024 Nov 4页

作者： Lianyu Lu Yaohui Wang Yue Ding Yuqing Wang Zhi Zhu Jinsong Lu Liu Yang Peng Zhang Chaoyong Yang Institute of Molecular Medicine Department of Breast Surgery Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127 P. R. China. The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation Key Laboratory for Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 P. R. China. Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. China.

The phenotype of circulating tumor cells (CTCs) offers valuable insights into monitoring cancer metastasis and recurrence. While microfluidics presents a promising approach for capturing these rare cells in blood, the phenotypic profiling of CTCs remains technically challenging. Herein, we developed a nanoporous micropillar array chip enabling highly efficient capture and in situ phenotypic analysis of CTCs through enhanced and tunable on-chip immunoaffinity binding. The nanoporous micropillar array addresses the fundamental limits in fluidic mass transfer, surface stagnant flow boundary effect, and interface topographic and multivalent reactions simultaneously within a single device, resulting in a synergistic enhancement of CTC immunocapture efficiency. The CTC capture efficiency increased by approximately 40% for cancer cells with low surface marker expressing. By manipulating fluidic velocity (hydrodynamic drag force) on the chip, a cell adhesion gradient was generated in the capture chamber, enabling individual CTCs with varying expression levels of epithelial cellular adhesion molecules to be immunocaptured at the corresponding spatial locations where equilibrium drag force is provided. The clinical utility of the nanoporous micropillar array was demonstrated by accurately distinguishing early and advanced stages of breast cancer and further longitudinally monitoring treatment response. We envision that the nanoporous micropillar array chip will provide an in situ capture and molecular profiling approach for CTCs and enhance the clinical application of CTC liquid biopsy.

关键词： circulating tumor cells liquid biopsy microfluidic device nanoporous micropillar array phenotypic profiling

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In-Flow Extraction of RNA in Extracellular Vesicles Using a Silicon-Based Microfluidic Device

In-Flow Extraction of RNA in Extracellular Vesicles Using a ...

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IEEE International Conference on Micro Electro Mechanical Systems

作者： Sihui Chen Xi Chen Jing Du Yi Zhang Hui Yang Laboratory of Biomedical Microsystems and Nano Devices Center for Bionic Sensing and Intelligence Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen CHINA CAS Key Laboratory of Health Informatics Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen CHINA

ISBN: (数字)9781665419123

ISBN: (纸本)9781665430241

Extracellular vesicles (EVs) transport proteins, signaling lipids, and nucleic acids from donor cells to recipient cells, therefore have been verified to serve as mediators of intercellular communications. EV-resident RNAs have been widely used as biomarkers of disease and prognostic indicators. Thus, in situ extraction of RNAs in a microfluidic flow is of great importance for developing integrated microsystems to realize on-chip PCR. This paper presents a silicon-based microfluidic device for solid-phase extraction of EV-resident RNAs. The microfluidic device contains micro-pillar arrays within an area of 0.25cm 2 on the silicon substrate, different structures with various surface-to-volume ratio (SVR) are investigated to compare their RNA extraction efficiencies. A 20% extraction efficiency is obtained and characterized by using a Nanodrop UV-Vis spectrophotometer. The proposed on-chip extraction of EV-resident RNAs would provide new approaches to develop integrated microfluidic systems to realize PCR for point-of-care applications.

关键词： Micromechanical devices RNA Point of care Silicon System-on-chip Extracellular Microfluidics

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Design, simulation and experimentation of a biomimetic wall-climbing robot with tracked spines

Design, simulation and experimentation of a biomimetic wall-...

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2020 IEEE International Conference on Power, Intelligent Computing and Systems, ICPICS 2020

作者： Shi, Jia Xu, Linsen Liu, Jinfu Cheng, Gaoxin Liang, Xingcan Liu, Lei Chen, Shouqi Xu, Hong Institute of Advanced Manufacturing Technology Hefei Institutes of Physical Science Cas University of Science and Technology of China Changzhou City Jiangsu Province China Institute of Advanced Manufacturing Technology Hefei Institutes of Physical Science Cas Key Laboratory of Biomimetic Sensing and Advanced Robot Technology Changzhou City Jiangsu Province China

ISBN: (数字)9781728198743

ISBN: (纸本)9781728198736

This paper presents a novel cooperative grasping wheeled wall-climbing robot with the ability to climb concrete, brick walls using tracked-spines arrays located around the track. The robot uses a combination of the crank-link mechanism and gear transmission to control the tracked spines on both sides of the robot to grasp the asperities. The kinematic and dynamic analysis of the crank-link mechanism and the simulation demonstrate that the motion state of the robot is stable in the climbing process. By means of wireless communication, the control information is transmitted to the main controller, which drives the motors to make each mechanism work together. Finally, the prototype is fabricated and several experiments are carried out to verify the climbing performance of the robot. © 2020 IEEE.

关键词： Biomimetics

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Layer-by-layer stacked graphene nanocoatings by Marangoni self-assembly for corrosion protection of stainless steel

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Chinese Chemical Letters 2021年第1期32卷 501-505页

作者： Chen Ye Yangguang Zhu Hongyan Sun Feiyue Chen Huifang Sun Wen Dai Qiuping Wei Li Fu Aimin Yu Shiyu Du Minghui Yang Liang-Feng Huang Jinhong Yu Nan Jiang Cheng-Te Lin Key Laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Materials Technology and Engineering(NIMTE)Chinese Academy of SciencesNingbo 315201China 100049 China Laboratory of Environmental Biotechnology School of Environmental and Civil EngineeringJiangnan UniversityWuxi 214122China Bionic Sensing and Intelligence Center(BSIC) Institute of Biomedical and Health EngineeringShenzhen Institutes of Advanced TechnologyChinese Academy of ScienceShenzhen 518055China College of Science Henan University of TechnologyZhengzhou 450001China Faculty of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China School of Materials Science and Engineering Central South UniversityChangsha 410083China College of Materials and Environmental Engineering Hangzhou Dianzi UniversityHangzhou 310018China Department of Chemistry and Biotechnology Faculty of ScienceEngineering and TechnologySwinburne University of TechnologyHawthorn VIC 3122Australia Ningbo Institute of Materials Technology and Engineering(NIMTE) Chinese Academy of SciencesNingbo 315201China

Graphene nanosheets are widely used in anti-corrosion polymeric coating as filler,owing to the excellent electrochemical inertness and barrier ***,as the arrangement of graphene nanosheets is difficult to form a perfect layered structure,polymeric coating with graphene nanosheets usually needs micron-scale thickness to ensure the enhancement of corrosion *** this work,layer-by-layer stacked graphene nanocoatings were fabricated on stainless steel by self-assembly based on Marangoni *** anti-corrosion property of graphene coatings were studied through Tafel polarization curves,electrochemical impedance spectroscopy and accelerated corrosion test with extra applied *** self corrosion current density of optimized three-layered graphene coated sample was one quarter of that of bare stainless *** the self corrosion potential of optimized sample is increased to-0.045 *** to the results,graphene nanocoatings composed of layered nanosheets exhibits good anticorrosion ***,the self-assembly method provide a promising approach to make layeredstructure coating for other researches about 2 D material nanosheets.

关键词： Self-assembly Graphene nanosheets Layer-by-layer stacking Electrochemical corrosion Anti-corrosion coating

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Ultrahigh Resolution On-Chip Separation System Using Negative Magnetophoresis

Ultrahigh Resolution On-Chip Separation System Using Negativ...

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IEEE International Conference on Micro Electro Mechanical Systems

作者： Lin Zeng Xi Chen Jing Du Zitong Yu Rongrong Zhang Yi Zhang Hui Yang Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen CHINA Center for Medical AI Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen CHINA CAS Key Laboratory of Health Informatics Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen CHINA

ISBN: (数字)9781665419123

ISBN: (纸本)9781665430241

A high-resolution magnetic separation microfluidic system used for the negative magnetophoretic separation of nanoparticles is presented in this paper. The chip can generate an ultrahigh gradient magnetic field greater than 10 5 T/m in the separation channel by integrating two on-chip magnetic pole arrays. A label-free separation of nanoparticle is achieved by this microfluidic system with a mixed sample of 0.2 μm and 1 μm polystyrene particles, where the recovery rate of 0.2 μm particles is 91.20%, and the purity reaches 94.72%. The experimental results show that the chip can efficiently separate nano-scale particles with ultrahigh resolution, and in future, it may develop into a versatile and robust tool for the separation and purification of nano-biological samples.

关键词： Nanoparticles Purification Magnetic separation Magnetic devices System-on-chip Nanobioscience Microfluidics

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Ultrasensitive Plasmonic Biosensing Based on Critical Tuning of Phase Change Materials

Ultrasensitive Plasmonic Biosensing Based on Critical Tuning...

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Asia Communications and Photonics Conference and Exhibition (ACP)

作者： Yuye Wang Yurui Hu Shuwen Zeng Aurelian Crunteanu Yi Zhang Hui Yang Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen China Center for Medical AI Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen China Laboratory Light Nanomaterials & Nanotechnologies -L2n University of Technologie of Troyes (UTT) & CNRS-EMR Troyes France XLIM Research Institute UMR7252 CNRS/University of Limoges Limoges France

Here, we have revealed the potential of exploiting phase change materials (PCM) for ultrasensitive plasmonic biosensing through simulations and theoretical analysis. We have demonstrated that the detection sensitivity of the plasmonic sensors can be optimized and significantly improved by precisely engineering the configuration of PCM on the plasmonic sensing substrate and inducing a giant lateral position shift. These results will provide new opportunities for the ultrasensitive detection of biomolecules in various application scenarios.

关键词： Phase change materials Sensitivity Refractive index Molecular biophysics Plasmons Sensors Biosensors

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Optofluidics in bio-imaging applications

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Photonics Research 2019年第5期7卷 532-542页

作者： SIHUI CHEN RUI HAO YI ZHANG HUI YANG Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence Center Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

Bio-imaging generally indicates imaging techniques that acquire biological information from living ***, the ability to detect, diagnose, and monitor pathological, physiological, and molecular dynamics is in great demand, while scaling down the observing angle, achieving precise alignment, fast actuation, and a miniaturized platform become key elements in next-generation optical imaging systems. Optofluidics, nominally merging optic and microfluidic technologies, is a relatively new research field, and it has drawn great attention since the last decade. Given its abilities to manipulate both optic and fluidic functions/elements in the micro-/nanometer regime, optofluidics shows great potential in bio-imaging to elevate our cognition in the subcellular and/or molecular level. In this paper, we emphasize the development of optofluidics in bio-imaging, from individual components to representative applications in a more modularized, systematic sense. Further, we expound our expectations for the near future of the optofluidic imaging discipline.

关键词： Optofluidics biological information molecular dynamics

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Analysis on the Environmental Adaptability of A Reconfigurable Wheel-Tracked Wheel

Analysis on the Environmental Adaptability of A Reconfigurab...

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IEEE International Conference on Mechatronics, robotics and Automation (ICMRA)

作者： Lei Liu Linsen Xu Jinfu Liu Shouqi Chen Gaoxin Cheng Jia Shi University of Science and Technology of China Hefei China Anhui Province Key Laboratory of Biomimetic Sensing and Advanced Robot Technology Hefei China

The following topics are dealt with: mobile robots; motion control; path planning; human-robot interaction; particle filtering (numerical methods); medical robotics; finite element analysis; robot vision; biomechanics... 详细信息

ISBN: (数字)9781728190327

ISBN: (纸本)9781728190334

关键词： Adaptation models Analytical models Tracking Roads Wheels Finite element analysis Strain

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Design and Analysis for the Axe Foot of Bio-inspired Anchoring robot

Design and Analysis for the Axe Foot of Bio-inspired Anchori...

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IEEE International Conference on Mechatronics and Automation

作者： Lei Liu Linsen Xu Jinfu Liu Jiajun Xu Shouqi Chen Gaoxin Cheng Hong Xu Jia Shi University of Science and Technology of China Hefei China Anhui Province Key Laboratory of Biomimetic Sensing and Advanced Robot Technology Hefei China

ISBN: (数字)9781728164168

ISBN: (纸本)9781728164175

Mooring location has become increasingly important with the rapid development and utilization of marine resources. The mooring system of the underwater mobile robot is required to be efficient and intelligent. This paper proposes a novel anchoring robot by imitating the excellent inhabitation functions and physiological structure of the razor clam. This bio-inspired robot can autonomously dig holes, anchor, and detach to improve the efficiency of mooring location in marine operations. Specifically, the axe foot of the robot plays an important role in these movements. It contains a screw-cone drill and two soft parts. The screw-cone drill is the main forced part while the robot is in the digging state and the rationality of drill design is proved by mechanics analysis. Moreover, the soft part, which is connected with the upper end of the drill, is analyzed to show the capability of obstacle avoidance. The rationality of the structural design and a good submarine anchoring function of the axe foot are verified by the above analysis.

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Confining photonic nanojet in a microwell on microsphere lens for highly efficient light focusing, signal amplification and quantitative detection

arXiv

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arXiv 2021年

作者： Zhang, Pengcheng Yan, Bing Gu, Guoqiang Yu, Zitong Chen, Xi Wang, Zengbo Yang, Hui Laboratory of Biomedical Microsystems and Nano Devices Bionic Sensing and Intelligence Center Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Science Shenzhen China School of Computer Science and Electronic Engineering Bangor University Dean Street Gwynedd BangorLL57 1UT United Kingdom

Dielectric microspheres or microbeads can squeeze light into the subwavelength scale via photonic nanojet (PNJ) focusing. This enables strong light-matter interactions within its focus and induces extraordinary effects such as enhancements in light emission, signal collection and various other applications. However, critical challenges exist on how to efficiently and precisely loading the studied objects into the desired location of the PNJ focusing, and subsequently measure the field and signal of interests precisely and reliably. Such technique is currently missing. We present for the first time a unique microwell-decorated microsphere lens (μ-well lens), with a semi-open microwell sample compartment directly fabricated on top of the microsphere lens. The μ-well lens confines PNJ in a semi-open microwell and allows passive trapping of individual micro-object into the PNJ focusing area with high efficiency and spatial accuracy. We demonstrate that individual fluorescent microsphere of different sizes can be readily introduced to the designated location with loading efficiency >70% and generates reproducible enhanced fluorescence signals with standard deviation better than that can be quantitatively measured. A comprehensive analysis on the optical properties of the μ-well lens reveals the synergistic effect of field enhancement and collection efficiency on the optical enhancement. We finally employ this special microsphere lens for fluorescent-bead-based biotin concentration analysis. The results suggest a greatly enhanced sensitivity and highly improved detection limit, opening the door for its application in highly sensitive and quantitative detection. The μ-well microsphere lens demonstrated here is promising for the development of next-generation on-chip signal amplification and quantitative detection systems with extremely high sensitivity and low detection limit;it also provides a platform for investigating a wide range of light-matter interaction processes.

关键词： Microspheres

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