The design of functional metallohydrogels is attractive but challenging. A rational approach is introduced for designing functional metallohydrogels using chiral ligands, a phenylalanine derivative with a pyridyl grou...
详细信息
The design of functional metallohydrogels is attractive but challenging. A rational approach is introduced for designing functional metallohydrogels using chiral ligands, a phenylalanine derivative with a pyridyl group ( l / d ‐PF). Intriguingly, the as‐prepared metallohydrogel exhibits excellent O 2 binding and activating properties. Insights into the O 2 binding pathway reveals the presence of a novel [( l + d )‐PF‐Cu 3+ ‐O 2 − ] species, which can efficiently reduce ferric cytochrome c with the reactive O 2 − by receiving an electron from reductant ascorbic acid. This study provides helpful instructions for developing new artificial systems with specific functions through the effective combination of chiral ligands with metal ions.
Understanding uranium-protein interaction is important for revealing the mechanism of uranyl ion(UO2+2)toxicity. In this study, we investigated the interaction between UO2+2and a quadruple mutant of cytochrome b5(E44/...
详细信息
Understanding uranium-protein interaction is important for revealing the mechanism of uranyl ion(UO2+2)toxicity. In this study, we investigated the interaction between UO2+2and a quadruple mutant of cytochrome b5(E44/48/56A/D60 A cyt b5, namely 4A cyt b5) by spectroscopic approaches. The four mutated negativelycharged surface residues of cyt b5 have been considered to be the interactive sites with cytochrome c(cyt c).Also, we studied the interaction between UO2+2and the protein-protein complex of 4A cyt b5-cyt c. The results were compared to the interaction between UO2+2and cyt b5, and the interaction between cyt c and cyt b5-cyt c complex, from previous studies. It was found that the interaction of UO2+2-cyt b5, i.e., uranyl ion binding to cyt b5 surface at Glu37 and Glu43 as previously proposed by molecular modeling, is regulated by both surface mutations of cyt b5 and its interacting protein partner cyt c. These provide valuable information on metal-protein-protein interactions and clues for understanding the mechanism of uranyl toxicity.
After publication of this article, the authors noticed that a N-C dimension error was unwillingly coded in the 3D NMR spectrum "***" processing script used to perform backbone assignments for this enzyme. Th...
After publication of this article, the authors noticed that a N-C dimension error was unwillingly coded in the 3D NMR spectrum "***" processing script used to perform backbone assignments for this enzyme. The authors noticed that some OBS, CAR and LAB values in the "***" had been switched in the y and z dimensions, probably resulting from a wrong NMRPipe selection when reading the Varian NMR experimental parameters. They have carefully re-processed, re-analyzed, re-assigned, in addition to checking all scripts to evaluate the extent of this processing error on the published assignments. Authors determined that the "***" error resulted in a significant number of incorrect backbone resonance assignments, requiring us to issue corrections in Figs. 2, 3 and 4 of this published manuscript, in addition to Table S1. New versions of these figures and table are provided below. The corresponding BMRB entry has also been revised. The authors note that these modifications do not change the global message, conclusions, and molecular dynamics simulations presented in this article. The authors are grateful to David N. Bernard (INRS) for help with finding and correcting these errors.
作者：
WHEELWRIGHT, SMScott M. Wheelwright
Ph.D. Product Manager Reagent Laboratory Chiron Corp. 4560 Horton Street Emeryville CA 94608REFERENCES Office of Technology Assessment. 1984. Commercial Biotechnology: An International Analysis p. 5.Gareil P. and Rosset R. 1982. La Chromatographie en Phase Liquide Preparative par Development par Elution. Analusis 10: 397.|ISI|ChemPort|Hayes R.H. and Wheelwright S.C. 1984. Restoring Our Competitive Edge: Competing Through Manufacturing. John Wiley & Sons New York.Whitney D.E. 1986. Real Robots Do Need Jigs. Harvard Business Review 64: 110.|ISI|Shockley W.B. in Reid T.R. 1984. The Chip: How Two Americans Invented the Microchip and Launched a Revolution p. 70. Simon and Shuster New York.Curling J. 1979. Large Scale Ion Exchange Chromatography of Proteins in Protein: Structure Function and Industrial Applications. E. Hofman W. Pfeil and H. Aurich (eds.) Pergamon Press New York.Porter P.S. and Semmens J.J. 1980. A Review of Virus and Protein Sorption by Ion Exchange Resins. Environment International 3: 311.|Article|ChemPort|Yamamoto S. et al. 1983. Ion Exchange Chromatography of Proteins. Biotechnology and Bioengineering 25: 1465.Fischer L. 1980. Gel Filtration Chromatography. Elsevier Amsterdam.Provender T. (ed.). Size Exclusion Chromatography. ACS Symposium Series 245 ACS Washington D.C.Yang C.M. and Tsao G.T. 1982. Packed-Bed Adsorption Theories and Their Application to Affinity Chromatography. Advances in Biochemical Engineering 25.Ghetie V. Mota G. and Sjoquist J. 1978. Separation of Cells by Affinity Chromatography. J. Immunol. Methods 21: 133.|Article|PubMed|ISI|ChemPort|Fausnaugh J.L. Kennedy L.A. and Regnier F.E. 1984. Comparison of Hydrophobic Interaction and Reversed-Phase Chromatography of Proteins. J. Chromatogr. 317: 141.|Article|PubMed|ISI|ChemPort|Van Oss C.J. et al. 1978. Repulsive van der Waals Interactions: Their Role in Various Separation Methods. Separation and Purification Methods 7: 245.|ChemPort|Toth-Martinez
暂无评论