检索结果-内蒙古大学图书馆

Dormancy heterogeneity among Arabidopsis thaliana seeds is linked to individual seed size

Plant Communications 2024年第2期5卷 215-227页

作者： Michal Krzyszton Sebastian P.Sacharowski Veena Halale Manjunath Katarzyna Muter Grzegorz Bokota Ce Wang Dariusz Plewczynski Tereza Dobisova Szymon Swiezewski Laboratory of Seeds Molecular Biology Institute of Biochemistry and BiophysicsPAS02-106 WarsawPoland Laboratory of Functional and Structural Genomics Centre of New TechnologiesUniversity of WarsawWarsawPoland Laboratory of Bioinformatics and Computational Genomics Faculty of Mathematics and Information ScienceWarsaw University of TechnologyWarsawPoland Labdeers Ltd. 680-01 BoskoviceCzech Republic

Production of morphologically and physiologically variable seeds is an important strategy that helps plants to survive in unpredictable natural ***,the model plant Arabidopsis thaliana and most agronomically essential crops produce visually homogenous *** automated phenotype analysis,we observed that small seeds in Arabidopsis tend to have higher primary and secondary dormancy levels than large *** analysis revealed distinct gene expression profiles between large and small *** seeds have higher expression of translation-related genes implicated in germination *** contrast,small seeds have elevated expression of many positive regulators of dormancy,including a key regulator of this process,the DOG1 *** in DOG1 expression are associated with differential production of its alternative cleavage and polyadenylation isoforms;in small seeds,the proximal poly(A)site is selected,resulting in a short mRNA ***,single-seed RNA sequencing analysis demonstrated that large seeds resemble DOG1 knockout mutant ***,on the single-seed level,expression of genes affected by seed size is correlated with expression of genes that position seeds on the path toward *** results demonstrate an unexpected link between seed size and dormancy phenotypes in a species that produces highly homogenous seed pools,suggesting that the correlation between seed morphology and physiology is more widespread than initially assumed.

关键词： seed size dormancy DOG1 Arabidopsis

来源：评论

学校读者我要写书评

暂无评论

Engagement of sialylated glycans with Siglec receptors on suppressive myeloid cells inhibits anticancer immunity via CCL2

引用

Cellular & Molecular Immunology 2024年第5期21卷 495-509页

作者： Ronja Wieboldt Michael Sandholzer Emanuele Carlini Chia-wei Lin Anastasiya Börsch Andreas Zingg Didier Lardinois Petra Herzig Leyla Don Alfred Zippelius Heinz Läubli Natalia Rodrigues Mantuano Laboratory for Cancer Immunotherapy Department of BiomedicineUniversity Hospital and University of BaselBaselSwitzerland. Functional Genomics Center Zurich ETH ZurichZurichSwitzerland. Bioinformatics Core Facility Department of BiomedicineUniversity of Basel and Swiss Institute of BioinformaticsBaselSwitzerland. Department of Thoracic Surgery University Hospital BaselBaselSwitzerland. Laboratory of Cancer Immunology Department of BiomedicineUniversity Hospital and University of BaselBaselSwitzerland. Division of Oncology University Hospital BaselBaselSwitzerland.

The overexpression of sialic acids on glycans,called hypersialylation,is a common alteration found in cancer *** glycans can enhance immune evasion by interacting with sialic acid-binding immunoglobulin-like lectin(Siglec)receptors on tumorinfiltrating immune ***,we investigated the effect of sialylated glycans and their interaction with Siglec receptors on myeloid-derived suppressor cells(MDSCs).We found that MDSCs derived from the blood of lung cancer patients and tumor-bearing mice strongly express inhibitory Siglec receptors and are highly *** murine cancer models of emergency myelopoiesis,Siglec-E knockout in myeloid cells resulted in prolonged survival and increased tumor infiltration of activated T *** suppressive myeloid cells by blocking Siglec receptors or desialylation strongly reduced their suppressive *** further identified CCL2 as a mediator involved in T-cell suppression upon interaction between sialoglycans and Siglec receptors on *** results demonstrated that sialylated glycans inhibit anticancer immunity by modulating CCL2 expression.

关键词： sialoglycans Sialic acid-binding immunoglobulin-like lectin tumor microenvironment myeloid derived suppressor cells

来源：评论

学校读者我要写书评

暂无评论

Developing a pipeline for identification,characterization and molecular editing of cis-regulatory elements:a case study in potato

引用

aBIOTECH 2025年第1期6卷 91-96页

作者： Min Wan Handan Xie Hongwei Guo Shenglin Jing Deying Zeng Bing Li Bo Zhu Zixian Zeng Department of Biological Science College of Life SciencesSichuan Normal UniversityChengdu 610101China Plant Functional Genomics and Bioinformatics Research Center Sichuan Normal UniversityChengdu 610101China Crop Research Institute Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan ProvinceSichuan Academy of Agricultural SciencesChengdu 610066China

Crop breeding requires a balance of tradeoffs among key agronomic traits caused by gene *** molecular manipulation of genes can effectively improve target traits,but this may not reduce gene pleiotropy,potentially leading to undesirable traits or even lethal ***,molecular editing of cis-regulatory elements(CREs)of target genes may facilitate the dissection of gene pleiotropy to fine-tune gene *** this study,we developed a pipeline,in potato,which employs open chromatin to predict candidate CREs,along with both transient and genetic assays to validate the function of CREs and CRISPR/Cas9 to edit candidate *** used StCDF1 as an example,a key gene for potato tuberization and identified a 288 bp-core promoter region,which showed photoperiodic inducibility.A homozygous CRISPR/Cas9-editing line was established,with two deletions in the core promoter,which displayed a reduced expression level,resulting in late tuberization under both longday and short-day *** pipeline provides an alternative pathway to improve a specific trait with limited downside on other phenotypes.

关键词： Cis-regulatory element Potato CRISPR Genome-editing

来源：评论

学校读者我要写书评

暂无评论

Single-cell transcriptome analysis dissects lncRNA-associated gene networks in Arabidopsis

引用

Plant Communications 2024年第2期5卷 94-105页

作者： Zhaohui He Yangming Lan Xinkai Zhou Bianjiong Yu Tao Zhu Fa Yang Liang-Yu Fu Haoyu Chao Jiahao Wang Rong-Xu Feng Shimin Zuo Wenzhi Lan Chunli Chen Ming Chen Xue Zhao Keming Hu Dijun Chen State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China Department of Bioinformatics College of Life SciencesZhejiang UniversityHangzhou 310058China Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education College of AgricultureYangzhou UniversityYangzhou 225009China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou UniversityYangzhou 225009China Zhejiang Zhoushan High School Zhoushan 316099China National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops Hubei Hongshan LaboratoryWuhan 430070China

The plant genome produces an extremely large collection of long noncoding RNAs(lncRNAs)that are generally expressed in a context-specific manner and have pivotal roles in regulation of diverse biological ***,we mapped the transcriptional heterogeneity of lncRNAs and their associated gene reg-ulatory networks at single-cell *** generated a comprehensive cell atlas at the whole-organism level by integrative analysis of 28 published single-cell RNA sequencing(scRNA-seq)datasets from juvenile Arabidopsis *** then provided an in-depth analysis of cell-type-related lncRNA signatures that show expression patterns consistent with canonical protein-coding gene *** further demon-strated that the cell-type-specific expression of lncRNAs largely explains their tissue *** addi-tion,we predicted gene regulatory networks on the basis of motif enrichment and co-expression analysis of lncRNAs and mRNAs,and we identified putative transcription factors orchestrating cell-type-specific expression of *** analysis results are available at the single-cell-based plant lncRNA atlas data-base(scPLAD;https://***/scPLAD/).Overall,this work demonstrates the power of inte-grative single-cell data analysis applied to plant lncRNA biology and provides fundamental insights into lncRNA expression specificity and associated gene regulation.

关键词： single-cell transcriptomics long noncoding RNAs lncRNAs gene regulatory networks GRNs plants

来源：评论

学校读者我要写书评

暂无评论

Aptamer blocking S-TLR4 interaction selectively inhibits SARS-CoV-2 induced inflammation

引用

Signal Transduction and Targeted Therapy 2022年第5期7卷 1383-1385页

作者： Gang Yang Shengnan Zhang Yuchun Wang Ling Li Yu Li Deyu Yuan Fatao Luo Jincun Zhao Xu Song Yongyun Zhao Center for Functional Genomics and Bioinformatics College of Life ScienceSichuan UniversityChengduSichuan 610064P.R.China State Key Laboratory of Respiratory Disease Guangzhou Institute of Respiratory DiseaseThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong 510182China

Excessive inflammatory responses lead to increased mortality from the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Nearly all deceased patients infected with SARS-CoV-2 are found to have cytokine storm syndrome and viral *** are prone to superinfections exacerbating the course of disease.1 Therefore,preventing hyperinflammation is critical for avoiding this cytokine storm ***,specific immunomodulators are under clinical research or application,including targeting inflammatory cytokines(sarilumab,anakinra,tocilizumab,infliximab,adalimumab)and inflammatory pathway(baricitinib,ruxolitinib).

关键词： inflammation mortality acute

来源：评论

学校读者我要写书评

暂无评论

PlantCFG:A comprehensive database with web tools for analyzing candidate flowering genes in multiple plants

引用

Plant Communications 2024年第2期5卷 11-14页

作者： Dongxu Liu Jiawei Li Shengbo Wang Tingting Huang Fangting Tao Yuchen Lin Wei Lin Xinle Zhao Yiming Huang Yupeng Jia Zhiquan Yang Chengfang Luo Qiang Zhu Wing-Kin Sung Jian Wu Qing-Yong Yang National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Hubei Key Laboratory of Agricultural Bioinformatics College of InformaticsHuazhong Agricultural UniversityWuhan 430070China Hong Kong Genome Institute Hong Kong Science ParkShatinHong KongChina State Key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhou 510275China School of Life Sciences University of Science and Technology of ChinaHefei 230026China Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou UniversityYangzhou 225009China

Dear Editor,The transition from the vegetative to the reproductive phase of plant development,known as the floral transition,plays important roles in the plant life *** is a vulnerable but crucial phase for building crop yield,and proper timing of this period is therefore vital for obtaining optimal yields(Jung and Muller,2009).Analysis of flowering time is currently an important research topic in several fields,including plant molecular genetics,evolutionary biology,ecology,and crop breeding(Huang et al.,2011).

关键词： database web breeding

来源：评论

学校读者我要写书评

暂无评论

Computational screening of potential drugs against COVID-19 disease: the Neuropilin-1 receptor as molecular target

引用

VirusDisease 2022年第1期33卷 23-31页

作者： Charoute, Hicham Elkarhat, Zouhair Elkhattabi, Lamiae El Fahime, Elmostafa Oukkache, Naoual Rouba, Hassan Barakat, Abdelhamid Research Unit of Epidemiology Biostatistics and Bioinformatics 1 Place Louis Pasteur Institut Pasteur du Maroc Casablanca 20360 Morocco Laboratory of Genomics and Human Genetics Institut Pasteur du Maroc Casablanca Morocco Molecular Biology and Functional Genomics Platform National Center for Scientific and Technical Research Rabat Morocco Laboratory of Venoms and Toxins Institut Pasteur du Maroc Casablanca Morocco

The transmembrane receptor Neuropilin-1 (NRP-1) was reported to serve as a host cell entry factor for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of COVID-19 disease. Therefore, molecular compounds interfering with SARS-CoV-2 binding to NRP-1 seem to be potential candidates as new antiviral drugs. In this study, NRP-1 receptor was targeted using a library of 1167 compounds previously analyzed in COVID-19 related studies. The results show the effectiveness of Nafamostat, Y96, Selinexor, Ebastine and UGS, in binding to NRP-1 receptor, with docking scores lower than − 8.2 kcal/mol. These molecules interact with NRP-1 receptor key residues, which makes them promising drugs to pursue further biological assays to explore their potential use in the treatment of COVID-19. © 2021, The Author(s), under exclusive licence to Indian Virological Society.

关键词： Antivirals COVID-19 Molecular docking Neuropilin-1 SARS-CoV-2 Virtual screening

来源：评论

学校读者我要写书评

暂无评论

The genomes of medicinal skullcaps reveal the polyphyletic origins of clerodane diterpene biosynthesis in the family Lamiaceae

引用

Molecular Plant 2023年第3期16卷 549-570页

作者： Haixiu Li Song Wu Ruoxi Lin Yiren Xiao Ana Luisa Malaco Morotti Ya Wang Meytal Galilee Haowen Qin Tao Huang Yong Zhao Xun Zhou Jun Yang Qing Zhao Angelos K.Kanellis Cathie Martin Evangelos C.Tatsis National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China Novogene Bioinformatics Institute BeijingChina Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai 201602 Group of Biotechnology of Pharmaceutical Plants Lab.of PharmacognosyDepartment of Pharmaceutical SciencesAristotle University of Thessaloniki54124 ThessalonikiGreece John Innes Centre Norwich NR47UHUK CEPAMS-CAS-JIC Centre of Excellence for Plant and Microbial Sciences Shanghai 200032China

The presence of anticancer clerodane diterpenoids is a chemotaxonomic marker for the traditional Chinese medicinal plant Scutellaria barbata,although the molecular mechanisms behind clerodane biosynthesis are ***,we report a high-quality assembly of the 414.98 Mb genome of *** into 13 *** phylogenomic and biochemical data,we mapped the plastidial metabolism of kaurene(gibberellins),abietane,and clerodane diterpenes in three species of the family Lamiaceae(Scutellaria barbata,Scutellaria baicalensis,and Salvia splendens),facilitating the identification of genes involved in the biosynthesis of the clerodanes,kolavenol,and *** show that clerodane biosynthesis evolved through recruitment and neofunctionalization of genes from gibberellin and abietane *** the assumed monophyletic origin of clerodane biosynthesis,which is widespread in species of the Lamiaceae,our data show distinct evolutionary lineages and suggest polyphyletic origins of clerodane biosynthesis in the family *** study not only provides significant insights into the evolution of clerodane biosynthetic pathways in the mint family,Lamiaceae,but also will facilitate the production of anticancer clerodanes through future metabolic engineering efforts.

关键词： Scutellaria barbata genome clerodane diterpenoids biosynthesis specialized metabolism evolution

来源：评论

学校读者我要写书评

暂无评论

Propene-bridged cyanurate tetramers decorated on carbon nanosheets with antibacterial activity: insights from molecular modeling and in vitro studies

RSC Applied Interfaces

引用

RSC Applied Interfaces 2024年第5期1卷 928-943页

作者： Agrawal, Omnarayan Sharma, Hitesh Kumar Chaurasia, Radhika Bakshi, Gaganjyot Kaur Agarwal, Aakanksha Sen, Mousumi Mamidala, Praveen Dey, R.K. Chourasia, Mukesh Mukherjee, Monalisa Amity Institute of Click Chemistry Research and Studies Amity University Uttar Pradesh Sector-125 U.P. Noida 201313 India Amity Institute of Pharmacy Amity University Uttar Pradesh Sector-125 U.P. Noida 201313 India Center for Computational Biology and Bioinformatics Amity Institute of Biotechnology Amity University Uttar Pradesh Noida 201301 India Amity Institute of Applied Sciences Amity University Uttar Pradesh Sector-125 U.P. Noida 201313 India Laboratory of Functional Genomics Department of Biotechnology Telangana University Nizamabad 503322 India Department of Chemistry Central University of Jharkhand Jharkhand Ranchi 835205 India

The increasing antibiotic resistance owing to the limitations of current antibiotics accentuates the imperative need for a trailblazing antibacterial therapy. Their unique physicochemical attributes make propene-bridged cyanurate tetramers decorated on carbon nanosheets (CNHs) promising materials for biomedical applications. In this study, we synthesized CNHs using cyanuric acid as the dopant and glycerol and sulphuric acid as the precursors and investigated their antibacterial activity against prominent bacterial strains, Escherichia coli and Staphylococcus aureus. By conducting in vitro experiments, we assessed the antibacterial efficacy of these materials. Additionally, by performing in silico molecular docking analysis, we unraveled the intricate interactions between CNH materials and their targets in bacterial cells. These studies confirmed the profound antibacterial effectiveness of CNHs against both the bacterial strains. In addition, molecular dynamics simulation of total 3 μs revealed the interaction of CNHs with the enzymes GlmU of E. coli and MurE of S. aureus, which are responsible for bacterial cell wall synthesis, resulting in the inhibition of bacterial growth. These findings manifest the potential of the CNH as a promising antibacterial agent. The unique physicochemical properties of the CNH, coupled with antibacterial activity, substantiate their promising roles across an expansive array of biomedical applications, prominently inclining towards the combat against debilitating bacterial infections. © 2024 RSC.

关键词：

来源：评论

学校读者我要写书评

暂无评论

T cell immune responses to SARS-CoV-2 and variants of concern(Alpha and Delta)in infected and vaccinated individuals

引用

Cellular & Molecular Immunology 2021年第11期18卷 2554-2556页

作者： Stanley C.Jordan Bong-Ha Shin Terry-Ann M.Gadsden Maggie Chu Anna Petrosyan Catherine N.Le Rachel Zabner Jillian Oft Isabel Pedraza Susan Cheng Ashley Vo Noriko Ammerman Jasmine Plummer Shili Ge Max Froch Anders Berg Mieko Toyoda Ruan Zhang Comprehensive Transplant Center Transplant Immunology LaboratoryCedars-Sinai Medical CenterLos AngelesCAUSA Division of Infectious Diseases Cedars-Sinai Medical CenterLos AngelesCAUSA Department of Cardiology Cedars-Sinai Medical CenterLos AngelesCAUSA Department of Bioinformatics&Functional Genomics Cedars-Sinai Medical CenterLos AngelesCAUSA Department of Pathology&Laboratory Medicine Cedars-Sinai Medical CenterLos AngelesCAUSA

Our understanding of immune responses to SARS-CoV-2 and variants of concern(VOCs)has been primarily acquired through analysis of Spike-specific IgG ***,a more comprehensive understanding of the breadth and longevity of immune responses after infection and vaccination requires analysis of cellular ***,we report on T cell immunity in infected and vaccinated individuals,identifying CD4+/CD8+T cell cytokine responses to SARS-CoV-2 and variant peptides(Alpha,B.1.1.7 and Delta,B.1.617.2).

关键词： Alpha infected immunity

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：