The extremophilic red microalga Cyanidioschyzon merolae suffers irreversible photosynthetic damage, oxidative stress, and apoptosis when exposed to Ni concentrations above 3 mM. To enhance Ni tolerance, we applied ad...
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The extremophilic red microalga Cyanidioschyzon merolae suffers irreversible photosynthetic damage, oxidative stress, and apoptosis when exposed to Ni concentrations above 3 mM. To enhance Ni tolerance, we applied adaptive laboratory evolution (ALE) and developed a robust strain capable of thriving at 10 mM Ni. The novel strain, dubbed 10NiLAD (10 mM Ni Long-term Adapted), exhibited growth, photosynthetic performance, as well as pigment and ATP content comparable to the wild type (WT), with c-phycocyanin content reaching 197 mg g. 10NiLAD strain accumulated lipids as cytoplasmic droplets with increased saturated fatty acids content, and showed significantly enhanced Ni biosorption (15 mg g). Notably, it produced significantly less reactive oxygen species than the WT, likely due to increased superoxide dismutase activity, suggesting a key role for this enzyme in oxidative stress mitigation under Ni stress. These results demonstrate the potential of ALE to generate metal-hyper resistant microalgal strains suitable for biotechnological applications in extreme environments.
Mitochondria change their morphology and distribution depending on the metabolism and functional state of a cell. Here, we analyzed the mitochondria and selected structures in female germ-line cysts in a representativ...
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