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Homogeneous label-free electrochemiluminescence biosensor based on double-driven amplification and magnetic graphene platform

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Biosensors and Bioelectronics: X 2022年 11卷

作者： Ni, Jiancong Liu, Liyang Dai, Xiaohui Huang, Danni Chen, Xiaoping Yang, Weiqiang Lin, Zhenyu Guo, Longhua Wang, Qingxiang Fujian Provincial Key Laboratory of Pollution Monitoring and Control Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology College of Chemistry and Environment Minnan Normal University Zhangzhou363000 China Zhangzhou Product Quality Inspection Institute Fujian Zhangzhou363000 China MOE Key Laboratory for Analytical Science of Food Safety and Biology Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety College of Chemistry Fuzhou University Fujian Fuzhou350116 China Jiaxing Key Laboratory of Molecular Recognition and Sensing College of Biological Chemical Sciences and Engineering Jiaxing University Zhejiang Jiaxing314001 China

Traditional DNA-based electrochemiluminescence (ECL) biosensors generally require a laborious and time-consuming process of electrode modification. Although immobilization-free homogeneous electrochemical approaches have been proposed, the problems of low detectable signals from indicators and high background interferences resulting from label-free sensors remain unsolved. Herein, a new type of homogeneous label-free ECL biosensor for aflatoxin B1 (AFB1) is designed based on a double-driven signal-amplifying strategy (combination of entropy-driven and nuclease-driven) and a background reduction by magnetic graphene oxide (M-GO). The target of AFB1 competitively binds the aptamer in the duplex DNA, leading to the release of the catalyst strand which would further bind the Ru(bpy)32+-Probe (Ru-Probe) absorbed in M-GO based on the principle of entropy increase. In the presence of exonuclease III, the Ru-Probe is degraded to release Ru(bpy)32+ for detection, and the catalyst strand is preserved to trigger much more Ru(bpy)32+ released. So the nuclease-driven combined with entropy-driven amplifying strategy greatly enhances the ECL signal. Moreover, the unreacted Ru-Probes adsorbed in M-GO are removed by the magnetic separation, providing the background signal was reduced as far as possible. Under the optimum conditions, the enhanced ECL signal exhibits a linear relationship with the AFB1 concentration in the range of 1 pM–100 nM with a detection limit of 20 fM. The homogeneous ECL biosensor is successfully applied to determine AFB1 in corn samples with high sensitivity and selectivity. By altering the probes employed, this type of novel ECL biosensor is promising for further determination of diverse targets which are trace contaminants in food, environment, and the disease biomarkers. © 2022 The Authors

关键词： Biosensors

Comparative Analysis of Nitrate Evolution Patterns During pollution Episodes: Method Development and Results from Tianjin, China

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SSRN 2022年

作者： Li, Yafei Han, Yan Ma, Simeng Zhang, Yufen Wang, Haoqi Yang, Jingyi Yao, Lu Bi, Xiaohui Wu, Jianhui Feng, Yinchang State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control Center for Urban Transport Emission Research College of Environmental Science and Engineering Nankai University Tianjin300350 China CMA-NKU Cooperative Laboratory for Atmospheric Environment-Health Research Tianjin300000 China Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing 400714 China

Nitrate plays an increasingly important role in the formation of pollution process, while the main mechanisms of nitrate concentration change are different in each stage, same as the driving factors. In this study, we proposed an episode-based analysis to illustrate the typical nitrate evolution patterns and identify the possible impacting factors in different evolution stages. Applying into twelve pollution episodes, three typical patterns of nitrate evolution were abstracted, and the corresponding conceptual models were constructed. All the pollution episodes were grouped by their evolving shapes, which were driven by physical and chemical processes. Type 1 ("hump-shaped type"), accounting for 66.3% of the total episode durations, including two "peak" concentrations, displays a rapid growth rate, mainly relying on the sharp drop in the planetary boundary layer height. Type 2 ("triangle-shaped type", 24.3%) shows a gentle growth rate and short duration. Both physical and chemical effects are weaker compared with Type 1. Type 3 ("trapezoid-shaped type", 9.4%) presents a long platform stage, during which the source and sink keep balance. Our study highlights the importance of pattern identification for understanding the nitrate evolution behavior, it may also provide insights for pollution prediction and scientific mitigation strategies. © 2022, The Authors. All rights reserved.

关键词： Nitrates

A New-Style Pohotoelectrochemical Sensing Device Based on Nh2-Uio-66@Bi2o3 for the Sensitive Detection of Hydrogen Sulfide

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SSRN 2024年

作者： Song, Zhiping Luo, Tingfang Ke, Jingru Hu, Shuisheng Yang, Weiqiang Ni, Jiancong Chen, Xiaoping Chen, Zhonghui Fujian Provincial Key Laboratory of Pollution Monitoring and Control Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology College of Chemistry Chemical Engineering and Environment Minnan Normal University Zhangzhou363000 China Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases Ministry of Education Key Laboratory for Tissue Engineering of Jiangxi Province Gannan Medical University Jiangxi Ganzhou341000 China Central Laboratory Affiliated Hospital of Putian University Putian University Putian351100 China

Hydrogen sulfide (H2S) has been regarded as an environmentally hazardous substance and its abnormal level in the atmosphere can cause great harm for human body. Therefore, the development of a rapid and sensitive method for H2S detection is valuable for both human health and ecosystem safety. Herein, a new-style photoelectrochemical (PEC) sensing device integrated with a headspace separation system for sensing H2S was constructed by assembling a NH2-UiO-66@Bi2O3 modified photoelectrode array which was prepared by a vacuum filtration technique. According to the experimental results, the H2S gas volatilized from the test sample induces partial in situ transformation of the NH2-UiO-66@Bi2O3 modified on photoelectrode array into NH2-UiO-66@Bi2S3 which promotes electron-holes separation and visible light absorption, thus leading to the enhancement of photocurrent response. Correspondingly, a signal-on PEC sensing device was thereby constructed for sensing H2S concentration linearly ranging from 2.5 to 100 µM, with a detection limit of about 0.78 µM. Beyond that, the feasibility of sensing H2S in environment samples was further confirmed by recovery assays. This work could broaden a new vision of the elegant design of portable PEC sensor for rapid and sensitive environmental analysis. © 2024, The Authors. All rights reserved.

关键词： Hydrogen sulfide

Impact of Clogging on Accumulation and Stability of Phosphorus in the Subsurface Flow Constructed Wetland

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SSRN 2022年

作者： Jin, Fenglin Hu, Zhen Liu, Huaqing Su, Jixin Zhang, Jian Wang, Shuo Zhao, Yanhui Shandong Key Laboratory of Water Pollution Control and Resource Reuse School of Environmental Science & Engineering Shandong University Qingdao266237 China College of Safety and Environmental Engineering Shandong University of Science and Technology Qingdao266590 China Field Monitoring Station of the Ministry of Education East Route of the South-to-North Water Transfer Project Shandong University Jinan250100 China

Substrate clogging is one of the major operation challenges of subsurface flow constructed wetlands (SSF-CWs). And the phosphorus (P) removal performance and stability of P accumulation of SSF-CWs would be varied with the development of substrate clogging. In this study, three horizontal SSF-CWs microcosms with different clogging degree were conducted to explore the mechanism of P accumulation behavior influenced by substrate clogging. Increase of clogging degree resulted in hydraulic retention time (HRT) diminution and adsorption sites increase, which jointly led to reduced P removal efficiency at low clogging degree (L-CW), however, higher P removal efficiency was obtained at high clogging degree (H-CW). Substrate adsorption was the primary removal pathway in all CW systems. It accounted for 77.86% of the P input in the H-CW, significantly higher than the control (60.08%). This was attributed to higher proportion of Fe/Al-P accumulated on the substrate of H-CW, since clogging aggravated the anaerobic condition and promoted the generation of Fe ions. The increase of clogging degree also elevated the release risk of the accrued P in SSF-CW, since Fe/Al-P was considered bioavailable and readily released under environmental disturbance. The obtained results provide new insights into the P transport and transformation in SSF-CWs and would be helpful to optimize the substrate clogging management. © 2022, The Authors. All rights reserved.

关键词： Phosphorus

Detection of SARS-CoV-2 and Its Mutated Variants Using RT-LAMP-CRISPR-Cas12a Platform

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Wuhan University Journal of Natural Sciences 2024年第5期29卷 453-460页

作者： WANG Shanshan YAN Jun DU Tongtong LIU Luo CHEN Xiong HAN Song YIN Jun PENG Biwen HE Xiaohua LU Xuan LIU Wanhong Hubei Province Key Laboratory of Allergy and Immunology Taikang Medical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071HubeiChina Department of Laboratory Medicine Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430016HubeiChina Beijing Bioprocess Key Laboratory Beijing University of Chemical TechnologyBeijing 100029China Key Laboratory of Environmental Pollution Monitoring and Disease Control(Guizhou Medical University) Ministry of EducationGuiyang 550025GuizhouChina

The global outbreak of coronavirus disease 19(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2),has raised significant global *** a rapid,efficient,sensitive,and accurate point-of-care detection method is imperative for curbing SARS-Co V-2 ***,we screened a sequence,designed a set of highly sensitive loopmediated isothermal amplification primers(LAMP)and g RNA,and developed a user-friendly detection platform combining CRISPRCas12a and RT-LAMP technology to specifically detect SARS-Co V-2 and its 5 *** analysis and Cas12a-g RNA identification ensured sequence specificity,allowing us to identify SARS-Co V-2 *** developed a method for the detection of SARSCoV-2 using these primers in combination with LAMP amplification and CRISPR-Cas12a *** method is designed to detect SARS-CoV-2(NC_045512),Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),Delta(B.1.617.2)and Omicron(B.1.1.529).Additionally,it can differentiate SARS-CoV-2 from other *** analysis can be conducted by measuring fluorescence values,while qualitative analysis can be performed by observing fluorescence color point-of-care diagnosis changes with the naked *** results suggest that a set of novel sensitive LAMP primers and g RNA have been obtained to detect the extensive variants,and the RT-LAMPCRISPR-Cas12a platform significantly facilitates point-of-care diagnosis,thereby halting the spread of SARS-Co V-2,thus contributing to COVID-19 prevention and control.

关键词： SARS-CoV-2 mutation CRISPR RT-LAMP point-of-care diagnosis

Optimization and Application Of Pretreatment Method of Microplastics Detection in Municipal Solid Waste Landfills

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SSRN 2024年

作者： Zheng, Saqi Zhou, Baiyu Guo, Nanfei Li, Naying Wu, Jialun Cheng, Yong Han, Zhiyong State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology Chengdu610059 China State Environmental Protection Key Laboratory of Synergetic Control Joint Remediation for Soil & Water Pollution Chengdu University of Technology Chengdu610059 China College of Ecology and Environment Chengdu University of Technology Chengdu610059 China YangJiang Nuclear Power Co. Ltd. Yangjiang529500 China Chengdu Ecological Environment Monitoring Center Station of Sichuan Province Chengdu610041 China

Landfills are one of the most important sources of microplastics (MPs). The pretreatment method is a precondition of microplastics study for the presence of complex substances in landfills. Therefore, it is essential to examine the impact of different pretreatment methods on the microplastics detection. A literature review and a comparison experiment on digestion solutions were performed to establish a comprehensive identification method for MPs in landfills. When exposed to of 30% H2O2, minimal mass reduction of PE, PP and PET were 4.00%, 3.00% and 3.00% respectively, and the least surface damage was observed in MPs, while exhibiting the most optimal peak value for infrared spectral characteristics. It is demonstrated that the effect of 30% H2O2 dissolution was superior compared to 10% KOH and 65% HNO3. The method was subsequently utilized to investigate the distribution of MPs in a landfill. The dominant MPs were polyethylene (PE, 18.56-23.91%), polyethylene terephthalate (PET, 8.80-18.66%), polystyrene (PS, 10.31-18.09%), and polypropylene (PP, 11.60-14.91%). The comprehensive identification method of "NaCl density separation + 30% H2O2 digestion + NaI density separation + sampling microscope + Mirco-FTIR" is suitable for MPs in landfills. © 2024, The Authors. All rights reserved.

关键词： Sodium chloride

Release and Utilization of Nitrogen and Phosphorus from Fertilizers Coated with Pig Manure Biochar and Struvite Derived from Swine Wastewater

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SSRN 2024年

作者： Lu, Tingtian Wu, Die Xu, Defu Li, Bing Wu, Liuying Yang, Hong Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Nanjing210044 China Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Nanjing210044 China School of Environmental Science and Engineering Nanjing University of Information Science &Technology Nanjing210044 China Joint International Research Laboratory of Climate and Environment Change Nanjing210044 China Department of Geography and Environmental Science University of Reading ReadingRG6 6AB United Kingdom

Inadequate treatment of large quantities of pig manure and swine wastewater has impacted environment and human health. In this study, various materials, including MgO modified biochar derived from pig manure (MgO-BC), biochar post nitrogen and phosphorus adsorption (MgO-BC+NP), struvite (MAP) extracted from swine wastewater, were employed to prepare five types of coated fertilizer. These comprised: 1) coated fertilizer solely with MgO-BC (MgBCF), 2) coated fertilizer exclusively with MgO-BC+NP (MgBC+NPF), 3) coated fertilizer only with MAP (MAPF), 4) coated fertilizer combining MgO-BC and MAP (MgBC+MAPF), 5) coated fertilizer blending MgO-BC+NP and MAP (MgBC+NP+MAPF). The release of phosphorus (P) from these coated fertilizers and its uptake by peas were investigated. The compressive strength of the MgBC+MAPF and MgBC+NP+MAPF coated fertilizers were 16.21 N/granule and 15.31 N/granule, respectively, outperforming the other coated fertilizers. Up to a leaching time of 36 h, the MgBC+NPF and MgBC+NP+MAPF coated fertilizers showcased the higher water absorption rates, but the MgBC+MAPF coated fertilizer exhibited a larger water absorption (p © 2024, The Authors. All rights reserved.

关键词： Phosphorus

Enhanced Removal Efficiency of Nitrogen and Phosphorus from Swine Wastewater Using Mgo Modified Pig Manure Biochar

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SSRN 2023年

作者： Wu, Liuying Xu, Defu Li, Bing Wu, Die Yang, Hong Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Nanjing210044 China Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Nanjing210044 China School of Environmental Science and Engineering Nanjing University of Information Science &Technology Nanjing210044 China Joint International Research Laboratory of Climate and Environment Change Nanjing210044 China Department of Geography and Environmental Science University of Reading ReadingRG6 6AB United Kingdom

Pig manure and swine wastewater from livestock and poultry contain high levels of nitrogen and phosphorus, which can seriously threaten ecosystems. In this study, biochar derived from pig manure (PMBC) was modified by various magnesium salt to obtain pig manure biochar MgO (MgO-PMBC), Mg(OH)2 (Mg(OH)2-PMBC) and MgCl2 (MgCl2-PMBC), and their capacities of simultaneous adsorption of NH4+-N and PO43-P were investigated. Results showed that the adsorption quantities for NH4+-N and PO43-P decreased in the order: MgO-PMBC > Mg(OH)2-PMBC > MgCl2-PMBC > PMBC. When the optimal dosage for MgO-PMBC was determined to be 0.6 g/L and the initial solution pH was set to the ideal value of 7, the maximum adsorption quantities for NH4+-N and PO43-P by MgO-PMBC reached 122.01 mg/g and 206.85 mg/g, respectively, which were 49.8 and 85.5 times of those of the PMBC. The specific surface area and total pore volume of MgO-PMBC were 8 times and 3 times of the PMBC. Removal rate of PO43-P from swine wastewater was 97.57%, and the concentration of PO43-P from treated swine wastewater was lower than 0.5 mg/L, the primary discharge standard of China’s Comprehensive Wastewater Discharge Standard. The enhanced simultaneous adsorption of NH4+-N and PO43-P by modified PMBC (MgO-PMBC) was ascribed to its greater specific surface area and total pore volume, and the reaction of crystallisation of struvite. Our results demonstrated that pig manure biochar modified by MgO can be used to treat swine wastewater effectively. © 2023, The Authors. All rights reserved.

关键词： Phosphorus

Enhancing rice production sustainability and resilience via reactivating small water bodies for irrigation and drainage (vol 14, 3794, 2023)

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NATURE COMMUNICATIONS 2023年第1期14卷 1-11页

作者： Li, Sisi Zhuang, Yanhua Liu, Hongbin Wang, Zhen Zhang, Fulin Lv, Mingquan Zhai, Limei Fan, Xianpeng Niu, Shiwei Chen, Jingrui Xu, Changxu Wang, Na Ruan, Shuhe Shen, Wangzheng Mi, Menghan Wu, Shengjun Du, Yun Zhang, Liang Hubei Provincial Engineering Research Center of Non-Point Source Pollution Control Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences Wuhan 430077 PR China Key Laboratory for Environment and Disaster Monitoring and Evaluation of Hubei Wuhan 430077 PR China University of Chinese Academy of Sciences Beijing 100049 PR China Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 PR China State Environmental Protection Key Laboratory of Soil Health and Green Remediation Huazhong Agricultural University Wuhan 430070 PR China Interdisciplinary Research Center for Territorial Spatial Governance and Green Development Huazhong Agricultural University Wuhan 430070 PR China Institute of Plant Protection Soil and Fertilizer Sciences Hubei Academy of Agricultural Sciences Wuhan 430064 PR China Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing 400714 PR China Liaoning Academy of Agricultural Sciences Shenyang 110161 PR China Institute of Soil & Fertilizer and Resources & Environment Jiangxi Academy of Agricultural Sciences Nanchang 330200 PR China

Rice farming threatens freshwater resources, while also being increasingly vulnerable to drought due to climate change. Rice farming needs to become more sustainable and resilient to climate change by improving irrigation drainage systems. Small water bodies, used to store drainage water and supply irrigation in traditional rice farming systems have gradually been abandoned in recent decades. This has resulted in a higher water footprint (WF) associated with rice farming due to increased freshwater usage and wastewater release, also leaving rice production more vulnerable to extreme weather events. Here, we propose how protecting and reactivating small water bodies for rice irrigation and drainage can decrease rice production WF in China by 30%, save 9% of China’s freshwater consumption, increase irrigation self-sufficiency from 3% to 31%, and alleviate yield loss in dry years by 2–3%. These findings show that redesigning rice irrigation drainage systems can help meet water scarcity challenges posed by climate change.

关键词： Environmental health Hydrology Wetlands ecology