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Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered ***

Bioresources and Bioprocessing 2024年第1期11卷 1340-1352页

作者： Kasimaporn Promubon Kritsada Tathiya Aussara Panya Wasu Pathom-Aree Pachara Sattayawat Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Cell Engineering for Cancer Therapy Research Group Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Master of Science Program in Applied Microbiology(International Program) Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand

Defining suitable enzymes for reaction steps in novel synthetic pathways is crucial for developing microbial cell factories for non-natural ***,we developed a computational workflow to identify C12 alcohol-active *** workflow involved three steps:(1)assembling initial candidates of putative UDP-glycosyltransferases,(2)refining selection by examining conserved regions,and(3)3D structure prediction and molecular *** sequences from Candida,Pichia,Rhizopus,and Thermotoga,known for lauryl glucoside synthesis via whole-cell biocatalysis,were *** of 240 predicted glycosyltransferases,8 candidates annotated as glycosyltransferases were selected after filtering out those with signal peptides and identifying conserved UDP-glycosyltransferase *** proteins underwent 3D structure prediction and molecular docking with ***3G,a candidate from Rhizopus delemar RA 99-880 with a relatively high ChemPLP fitness score,was selected and expressed in Escherichia coli BL21(DE3).It was further characterized using a feeding experiment with *** confirmed that the RO3G-expressing strain could convert 1-dodecanol to lauryl glucoside,as quantified by HPLC and identified by targeted *** the growth and fermentation profiles of the engineered strain revealed that RO3G expression did not affect cell ***,acetate,a major fermentation product,was reduced in the RO3G-expressing strain compared to the GFP-expressing strain,suggesting a redirection of flux from acetate to other ***,this work presents a successful workflow for discovering UDP-glycosyltransferase enzymes with confirmed activity toward 1-dodecanol for lauryl glucoside production.

关键词： UDP-glycosyltransferase Lauryl glucoside Computational method 1-Dodecanol Engineered E.coli Novel biosynthetic pathway Genome mining

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Atmospheric clearing of solid-particle debris using femtosecond filaments

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Physical Review Applied 2024年第1期22卷 014075-014075页

作者： Kyle S. Latty Kyle C. Hartig Nuclear Engineering Program Department of Materials Science and Engineering

Through nonlinear self-focusing, femtosecond pulses can propagate several kilometers beyond diffraction limits, forming an ionization channel in air known as a laser filaments. It has been demonstrated that in the wake of the filament, aerosols can be effectively cleared to improve the transmission of subsequent laser pulses or secondary light sources, pertinent to applications in atmospheric sensing. However, the current understanding of aerosol clearing is founded on interactions with droplets to simulate fogs and clouds and thus does not extend to solid particles or atmospheric debris. Using optical trapping, we isolate both graphite and silica microparticles and directly measure the subsequent displacement caused by the filament using time-resolved shadowgraphy. The shock wave from the filament is demonstrated to propel particles away from the filament, directly contributing to atmospheric debris clearing. Particles exposed to the laser light in either the intense filament core or the surrounding energy reservoir are axially displaced along the beam path. It is found that the optomechanical properties of the particle largely influence the axial displacement induced by laser exposure through mechanisms such as radiation pressure, mass ejection from ablation or optical damage, and particle deagglomeration.

关键词： Atmospheric science Light-matter interaction Microparticles Self-focusing Shock waves & discontinuities in plasma Aerosols Femtosecond laser irradiation Optical tweezers

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Historical, Contemporary, and Future Perspectives on Fuel Qualification

Historical, Contemporary, and Future Perspectives on Fuel Qu...

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2024 Annual Conference on Transactions of the American nuclear Society, ANS 2024

作者： Mika, Mitch Aitkaliyeva, Assel University of Florida Department of Materials Science Nuclear Engineering Program GainesvilleFL United States

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Automating selective area electron diffraction phase identification using machine learning

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Journal of Materiomics 2024年第4期10卷 896-905页

作者： M.Mika N.Tomczak C.Finney J.Carter A.Aitkaliyeva Nuclear Engineering Program in Department of Materials Science University of Florida100 Rhines HallGainesville32611FLUSA Department of Materials Science&Engineering Case Western Reserve University2111 Martin Luther King Jr DrCleveland44106OHUSA

Selective area electron diffraction(SAED)patterns can provide valuable insight into the structure of a ***,the manual identification of collected patterns can be a significant bottleneck in the overall phase classification *** this work,we utilize the recent advances in computer vision and machine learning(ML)to automate the indexing of SAED *** performance of six different ML algorithms is demonstrated using metallic plutonium-zirconium *** most successful approach trained a neural network(NN)to make a classification of the phase and zone axis,and then utilized a second NN to synthesize multiple independent predictions of different tilts in a single sample to make an overall phase *** results demonstrate that automated SAED phase identification using ML is a viable route to accelerate materials characterization.

关键词： Selective area electron diffraction Machine learning Phase identification Metallic fuels Pu alloys

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Distance Learning at the MS and PhD Levels in the nuclear and Radiation engineering program, nuclear and Applied Robotics, Materials science and engineering and Walker department of Mechanical engineering at the University of Texas at Austin

Distance Learning at the MS and PhD Levels in the Nuclear an...

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2023 Transactions of the American nuclear Society Winter Conference and Expo, ANS 2023

作者： Landsberger1, Sheldon Charlton, William Clamo, Kevin Ezekoye, Ofodike Kovar, Desiderio Haas, Derek Pryor, Mitchell University of Texas at Austin Nuclear and Radiation Engineering Program Nuclear Engineering Teaching Lab 10100 Burnet Road Pickle Research Campus R-9000 AustinTX United States Walker Department of Mechanical Engineering University of Texas at Austin 204.Dean Keeton ETC II 5.160 AustinTX United States The University of Texas at Austin Materials Science and Engineering Walker Department of Mechanical Engineering 204 Dean Keeton ETC II 5.100 AustinTX United States

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A ShuffleNet Architecture-Based Multi-Layer Feature Scaling and Fusion Approach for Underwater Net Cage Damage Detection in Aquaculture

A ShuffleNet Architecture-Based Multi-Layer Feature Scaling ...

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2024 IEEE International Conference on Consumer Electronics-Asia, ICCE-Asia 2024

作者： Huang, Xiang-Rui Chen, Yi-Cheng Chen, Liang-Bi Hu, Wu-Chih National Penghu University of Science and Technology Master Program in Electrical Engineering and Computer Science Department of Electrical Engineering Penghu Taiwan National Penghu University of Science and Technology Department of Computer Science and Information Engineering Penghu Taiwan

ISBN: (纸本)9798331530839

In offshore aquaculture operations, personnel equipped with diving gear are often necessary to inspect the underwater net cages for damage, particularly on the sea floor. This manual inspection process is time-consuming and complex. To overcome this problem, this paper proposes a computer vision solution for identifying damage in underwater net cages to address the inefficiencies and challenges of traditional manual inspections. The proposed scheme utilizes a high-performance multi-branch computational architecture designed based on ShuffleNet architecture to detect net cage damage more efficiently. Experimental results demonstrate that this work performs well on the ImageNet ILSVRC-2010 dataset and achieves an accuracy of 88.54% in underwater net damage detection. © 2024 IEEE.

关键词： Aquaculture

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Evaluated Material Attractiveness of Plutonium Composition from Economic Simplified Boiling Water Reactor (ESBWR) 10

Evaluated Material Attractiveness of Plutonium Composition f...

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10th Asian Physics Symposium 2023, APS 2023

作者： Nasyidiah, Fungky Iqlima Permana, Sidik Pramuditya, Syeilendra Master Program in Nuclear Science and Engineering Faculty of Mathematics and Natural Sciences Institute Technology of Bandung Ganesa 10th Street Bandung40132 Indonesia Department of Nuclear Science and Engineering Faculty of Mathematics and Natural Sciences Institute Technology of Bandung Ganesa 10th Street Bandung40132 Indonesia

The potential for misuse of nuclear fuel has prompted many to make efforts to prevent a country from acquiring nuclear explosive devices (weapons). Weapon utility equated with nuclear material attractiveness when applied to protection and security. Material attractiveness evaluated using an equation first developed by Masaki Saito, namely ATTR. ESBWR is one of the reactors of the BWR reactor type that is still operating. The purpose of this study is to evaluate Material Attractiveness based on the composition of plutonium in the ESBWR reactor, during reactor operation and after reactor operation. The ATTR concept utilizes the plutonium isotope composition to evaluate the material attractiveness aspect. Parameters such as BCM, Rossi-alpha, and neutron prompt life become additional aspects in evaluating material attractiveness. In the research results, the ATTR value decreased from the beginning of reactor operation until the reactor stopped operating. At the beginning of the reactor operation, it was categorizing as a weapon-grade with an ATTR value of 0.19 and when the reactor stopped operating it was categorizing as an unusable grade with an ATTR value of 0.012. At the time of cooling time up to 1x106 (one million) cooling time, the ATTR value increases by 0.019 which was categorizing as an unusable grade. After a cooling time of 1x106 (one million) years up to 1x107 (ten million) years, the ATTR value was 0.001 which categorized as an exempt level. This states that the increasing burnup value will further reduce the ATTR value. Likewise with the Rossi-Alpha and Bare Critical Mass values. Conversely, it will decrease the value of neutron lifetime. The ATTR value after the reactor operates up to one hundred years of cooling time increases and then decreases significantly after 1x106 (one million) years due to the half-life of plutonium isotopes. Likewise, the Rossi-Alpha and Bare critical mass values. However, it is different with the neutron fast lifetime. © Publis

关键词： Boiling water reactors

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Design of an Automatic Price Tag System for Web-Based Retail Business 9

Design of an Automatic Price Tag System for Web-Based Retail...

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9th International Conference on Information Management and Technology, ICIMTech 2024

作者： Liawatimena, Suryadiputra Gunawan, Devina Bina Nusantara University Automotive & Robotics Program Computer Engineering Department BINUS ASO School of Engineering Computer Science Deparment BINUS Graduate Program Master of Computer Science Jakarta11480 Indonesia Bina Nusantara University Automotive & Robotics Program Computer Engineering Department BINUS ASO School of Engineering Jakarta11480 Indonesia

ISBN: (纸本)9798350390025

Modern retail businesses face a significant challenge with the inefficiency of manually changing price labels on shelves. This manual process not only consumes valuable time and resources but also increases the likelihood of errors, leading to potential inaccuracies in pricing and a less streamlined shopping experience for customers. The proposed solution is the implementation of an Electronic Shelf Label (ESL) system that automatically displays the prices of goods on retail business shelves. This system connects a website (front end and back end) to e-paper via a Wi-Fi network and microcontroller, allowing retail business owners to update prices more easily. Additionally, buyers can search for desired items through the website. The results are the time to send data from the website to the e-paper, namely, to know the performance of the e-paper used. The average time required from 10 attempts to send data from the website to the e-paper is 20.935 seconds. Since the data is connected to the server and will be updated automatically, using this system will be more efficient than manually changing the price label, although it takes time to transfer data from the website to the e-paper. © 2024 IEEE.

关键词： Sales

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RUL Estimation for Power Electronic Devices Using RNNs

RUL Estimation for Power Electronic Devices Using RNNs

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2024 Prognostics and System Health Management Conference, PHM 2024

作者： Cai, Congrui Lu, Zhonghai School of Electrical Engineering and Computer Science KTH Royal Institute of Technology Embedded Systems Master Program Sweden School of Electrical Engineering and Computer Science Department of Electrical Engineering KTH Royal Institute of Technology Sweden

ISBN: (纸本)9798350360585

Recurrent Neural Networks (RNNs) are commonly used in data-driven approaches to estimate the Remaining Useful Lifetime (RUL) of power electronic devices. RNNs are preferred because their intrinsic feedback mechanisms are better suited to model time-series data. However, the impact of RNN complexity on estimation accuracy is rarely discussed in the literature. This issue is important because choosing a lower-complexity model that delivers the same or similar performance as a higher-complexity model can increase implementation efficiency. In the paper, we use three RNN models, namely, the vanilla version, LSTM (Long Short Term Memory) and GRU (Gated Recurrent Unit) to conduct RUL estimation for power electronic devices. We use two accelerated aging datasets, one dataset targeting the package failure of MOSFETs, and the other dataset targeting package failure of power diodes. Our study shows that a lower-complexity RNN does not necessarily deliver a lower performance. Similarly, a higher-complexity model does not assure a higher performance. As such, our work highlights the importance of selecting a proper neural network for RUL estimation not biased towards complex models. This is especially useful and important for implementing such RUL estimation techniques in embedded resource-constrained and speed-limited computins platforms. © 2024 IEEE.

关键词： Long short-term memory

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Neutronic and Proliferation Resistance Analysis of Small Modular Pressurized Water Reactor with Various Fuel Types using SRAC 2006 10

Neutronic and Proliferation Resistance Analysis of Small Mod...

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10th Asian Physics Symposium 2023, APS 2023

作者： Amatullah, Amila Permana, Sidik Pramuditya, Syeilendra Master Program in Nuclear Science and Engineering Faculty of Mathematics and Natural Sciences Bandung Institute of Technology Ganesha No. 10 Bandung Indonesia Graduate Program in Nuclear Science and Engineering Faculty of Mathematics and Natural Sciences Bandung Institute of Technology Ganesha No. 10 Bandung Indonesia Graduate Program in Physics Faculty of Mathematics and Natural Sciences Bandung Institute of Technology Ganesha No. 10 Bandung Indonesia

One strategy for emission reduction in Indonesia is the dedieselization program, replacing diesel power plants with renewables. Pressurized Water Reactor (PWR) type Small Modular Reactor (SMR) is also a consideration. This study aims to compare fuel performance in SMRs. Four fuel types with varying fissile and fertile nuclides but equal fissile mass percentages were assessed: U235- U238, U235-Th232, U233-U238, and U233-Th232, using PIJ SRAC 2006 and JENDL 4.0. Parameters include neutron multiplication factor, conversion ratio, and isotope production. Results indicated that U233-U238 has a highest initial neutron multiplication factor, 1.55. Substituting uranium-238 with thorium-232 boosts neutron flux and conversion, reducing nuclear proliferation risk. While initial neutron multiplication may not be highest, thorium-232 can produce uranium-233 with high initial neutron multiplication factor. This research aids fuel selection for Indonesia SMR dedieselization program. © Published under licence by IOP Publishing Ltd.

关键词： Thorium

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