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Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered ***

Bioresources and Bioprocessing 2024年第1期11卷 1340-1352页

作者： Kasimaporn Promubon Kritsada Tathiya Aussara Panya Wasu Pathom-Aree Pachara Sattayawat Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Cell Engineering for Cancer Therapy Research Group Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Master of Science Program in Applied Microbiology(International Program) Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand

Defining suitable enzymes for reaction steps in novel synthetic pathways is crucial for developing microbial cell factories for non-natural ***,we developed a computational workflow to identify C12 alcohol-active *** workflow involved three steps:(1)assembling initial candidates of putative UDP-glycosyltransferases,(2)refining selection by examining conserved regions,and(3)3D structure prediction and molecular *** sequences from Candida,Pichia,Rhizopus,and Thermotoga,known for lauryl glucoside synthesis via whole-cell biocatalysis,were *** of 240 predicted glycosyltransferases,8 candidates annotated as glycosyltransferases were selected after filtering out those with signal peptides and identifying conserved UDP-glycosyltransferase *** proteins underwent 3D structure prediction and molecular docking with ***3G,a candidate from Rhizopus delemar RA 99-880 with a relatively high ChemPLP fitness score,was selected and expressed in Escherichia coli BL21(DE3).It was further characterized using a feeding experiment with *** confirmed that the RO3G-expressing strain could convert 1-dodecanol to lauryl glucoside,as quantified by HPLC and identified by targeted *** the growth and fermentation profiles of the engineered strain revealed that RO3G expression did not affect cell ***,acetate,a major fermentation product,was reduced in the RO3G-expressing strain compared to the GFP-expressing strain,suggesting a redirection of flux from acetate to other ***,this work presents a successful workflow for discovering UDP-glycosyltransferase enzymes with confirmed activity toward 1-dodecanol for lauryl glucoside production.

关键词： UDP-glycosyltransferase Lauryl glucoside Computational method 1-Dodecanol Engineered E.coli Novel biosynthetic pathway Genome mining

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Economic Feasibility Analysis and Breakeven Point Estimation of Microgrid System Combinations Under Carbon Fee Policies 4

Economic Feasibility Analysis and Breakeven Point Estimation...

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4th IEEE International Conference on Recent Advances in Systems science and engineering, RASSE 2024

作者： I-Hua, Chung Chin-Pao, Chu Ming-Jun, Zhang Master's Program in Offshore Wind Energy Engineering National Kaohsiung University of Science and Technology Kaohsiung Taiwan

ISBN: (数字)9798331528263

ISBN: (纸本)9798331528263

This study delves into the economic feasibility of various energy system combinations under the influence of carbon fee policies, using a convenience store located in the Changbin Industrial Park as a representative case. The primary focus is analyzing the intricate relationship between the Levelized Cost of Energy (LCOE) and carbon fees. In light of the growing importance of sustainable energy practices, this research explores how different combinations of renewable energy sources and storage systems can be optimized within the context of evolving carbon fee regulations. The findings underscore the pivotal role of carbon fees as a decisive factor in determining the economic viability of microgrid systems. Specifically, when the carbon fee escalates to NT$3000/tCO2e, certain system configurations, such as GPWL (Grid-Photovoltaic-Wind-Lithium) and GPWF (Grid-Photovoltaic-Wind-Flow Battery), emerge as economically viable options. However, the feasibility of these systems is significantly influenced by the prevailing costs of green electricity procurement, which can either enhance or limit their attractiveness. Moreover, the study introduces a regression analysis model capable of accurately predicting fluctuations in energy costs across different carbon fee scenarios. This model provides a robust analytical tool for decision-makers, enabling them to anticipate the financial implications of policy changes on energy systems. The insights derived from this research are particularly valuable for businesses and policymakers, offering a hopeful vision of a future with cost-effective and sustainable energy solutions. © 2024 IEEE.

关键词： Clean energy

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Impact of Energy Architecture on Assessing Microgrid Construction and Its Economic Benefits for Convenience Stores 7

Impact of Energy Architecture on Assessing Microgrid Constru...

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7th IEEE Eurasian Conference on Educational Innovation, ECEI 2024

作者： Chung, I-Hua Chu, Chin-Pao Zhang, Ming-Jun National Kaohsiung University of Science and Technology Master's Program in Offshore Wind Energy Engineering Kaohsiung Taiwan

ISBN: (纸本)9798350307207

For zero carbon emission, the industry has the pressure of carbon emission reduction. The service industry must also correspond to carbon emission policies. Hence, operators of convenience stores must prioritize the effective reduction of carbon emissions. There are two ways for them to reduce carbon emissions: energy conservation and renewable energy microgrids. Using HomerPro, we simulated the application of a renewable energy microgrid in convenience stores. Then microgrid system combinations were composed of the power grid, wind power, solar photovoltaics, lithium, and flow batteries. We used the 2030 carbon fee set in the Ministry of Environment report as the benchmark for techno-economic assessment to explore the carbon reduction benefits and cost assessments brought by the microgrid system. © 2024 IEEE.

关键词： Carbon

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Analysis of Renewable Energy Potential Combinations in Microgrid Systems 4

Analysis of Renewable Energy Potential Combinations in Micro...

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4th IEEE International Conference on Recent Advances in Systems science and engineering, RASSE 2024

作者： I-Hua, Chung Chin-Pao, Chu Ming-Jun, Zhang Master's Program in Offshore Wind Energy Engineering National Kaohsiung University of Science and Technology Kaohsiung Taiwan

ISBN: (数字)9798331528263

ISBN: (纸本)9798331528263

In response to the growing global and local demand for net-zero carbon emissions, this study thoroughly explores the feasibility of implementing microgrid technology in convenience stores across Taiwan to reduce carbon emissions and enhance overall energy sustainability significantly. The research emphasizes the integration of renewable energy sources, specifically solar and wind power, aligning with Taiwan's strategic initiatives in promoting renewable energy development. Utilizing the HomerPro simulation tool, the study conducts an in-depth assessment of various energy combinations, evaluating their performance under various conditions, including different carbon pricing scenarios, grid reliability factors, and essential financial variables such as discount rates and inflation. A total of 256 system configurations were simulated to identify the most economically viable and environmentally sustainable solutions for these convenience stores. The study provides a comprehensive understanding of the impact of these variables on the financial and operational feasibility of microgrid systems. The findings are crucial for informing policymakers, energy planners, and business owners, offering practical guidance for designing and implementing effective energy strategies within Taiwan's convenience store sector. By advocating for the adoption of microgrid technology, this study contributes to Taiwan's broader goals of achieving carbon neutrality and improving energy security and plays a key role in reducing dependence on conventional grid systems. This work supports transitioning to a more sustainable, resilient, and future-proof energy infrastructure. © 2024 IEEE.

关键词： Zero-carbon

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Improving Design Concepts for Overtemperature System Protection of Transformer Through Analysis of System Events Following an Accident 7

Improving Design Concepts for Overtemperature System Protect...

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7th IEEE Eurasian Conference on Educational Innovation, ECEI 2024

作者： Chung, I-Hua National Kaohsiung University of Science and Technology Master's Program in Offshore Wind Energy Engineering Kaohsiung Taiwan

ISBN: (纸本)9798350307207

The project staff investigates and discovers if the output contact of the protection relay fails in an electrical accident. In a short-circuit accident, the protection relay may not issue commands to fix the fault by activating the switch. The investigation result can be used to find the primary cause of the accident and why the equipment was damaged. In replacing the faulty protection relay, all newly installed relays must be tested to verify the functionality of their input and output contacts. Precautionary measures need to be ensured to prevent a similar incident. Poor equipment quality and improper installation cause a newly added power system's protection relay not to output signals correctly. Despite general procedures of testing the relay's output and input contacts before integrating the relay into the grid, accidents caused by the abovementioned reasons are still unavoidable. In this study, an in-depth analysis of the data logs and relevant protection relay settings was conducted, focusing on reliability and failure prevention. The analysis result revealed that the protection relay of transformer temperature was set conservatively, failing the backup protection. The case study results and discussions in this study provide a reference for power engineering designers and a guiding framework for accident analysis. © 2024 IEEE.

关键词： Timing circuits

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Cost-Effectiveness Analysis of Solar and Wind Power Generation in Microgrid Systems: A Case Study of Changbin Industrial Zone 4

Cost-Effectiveness Analysis of Solar and Wind Power Generati...

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4th IEEE International Conference on Recent Advances in Systems science and engineering, RASSE 2024

作者： I-Hua, Chung Chin-Pao, Chu Ming-Jun, Zhang Master's Program in Offshore Wind Energy Engineering National Kaohsiung University of Science and Technology Kaohsiung Taiwan

ISBN: (数字)9798331528263

ISBN: (纸本)9798331528263

The study centers on a comprehensive cost-effectiveness analysis of solar and wind power generation within the microgrid system located in the Changbin Industrial Zone. This region, characterized by favorable solar irradiance and wind conditions, is an ideal location for developing and implementing renewable energy systems. Through extensive simulations, we examined the impact of temperature fluctuations on photovoltaic (PV) module efficiency and the influence of varying wind turbine heights on energy output. Our findings revealed that temperature plays a crucial role in determining the performance of PV modules, with higher temperatures leading to a noticeable decline in efficiency. Conversely, wind power generation is significantly affected by the height of wind turbines, where increased height results in enhanced energy capture due to stronger and more consistent wind speeds at elevated altitudes. Furthermore, the study underscores the importance of considering seasonal variations, as these can significantly influence the overall efficiency and reliability of renewable energy systems within a microgrid. By optimizing wind turbine heights and incorporating strategies to mitigate the effects of temperature fluctuations on solar panels, the study demonstrates that it is possible to significantly improve the overall power generation efficiency of the microgrid system. Improving efficiency reduces the cost per kilowatt-hour of electricity generated, thereby enhancing the economic viability of renewable energy projects. The insights gained from this analysis are expected to provide valuable guidance for future renewable energy planning, particularly in regions with similar climatic and geographical conditions. Such strategic planning is essential for maximizing the return on investment and ensuring the long-term sustainability of renewable energy systems. © 2024 IEEE.

关键词： Windmill

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Scaled Component Models for Wind Farm Power Analysis Based on Direct-Drive Wind Turbines 7

Scaled Component Models for Wind Farm Power Analysis Based o...

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7th IEEE Eurasian Conference on Educational Innovation, ECEI 2024

作者： Chung, I-Hua Zhang, Ming-Jun Chu, Chin-Pao National Kaohsiung University of Science and Technology Master's Program in Offshore Wind Energy Engineering Kaohsiung Taiwan

ISBN: (纸本)9798350307207

In the past, gearboxes were used in wind turbines to adjust the rotor rotational speed. With advancements in the technology of wind turbines, wind farm developers adopt direct-drive wind turbines based on permanent magnet synchronous generators. In the power quality analysis in wind farms, the wind turbine model is used to simulate the electrical characteristics of wind turbines to meet regulatory requirements. However, it takes considerable time for a detailed analysis and assessment of wind turbine models in the early stages of large wind farms. Therefore, based on direct-drive wind turbines, we used power systems computer aided design (PSCAD) to construct several wind turbine units (SWTU) and scale component models (SCM). The model's performance was explored regarding tip speed ratio, pitch angle, and output power at different wind speeds. The practicality and performance of aggregated models were evaluated effectively in power system analysis. © 2024 IEEE.

关键词： Wind turbines

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A ShuffleNet Architecture-Based Multi-Layer Feature Scaling and Fusion Approach for Underwater Net Cage Damage Detection in Aquaculture

A ShuffleNet Architecture-Based Multi-Layer Feature Scaling ...

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2024 IEEE International Conference on Consumer Electronics-Asia, ICCE-Asia 2024

作者： Huang, Xiang-Rui Chen, Yi-Cheng Chen, Liang-Bi Hu, Wu-Chih National Penghu University of Science and Technology Master Program in Electrical Engineering and Computer Science Department of Electrical Engineering Penghu Taiwan National Penghu University of Science and Technology Department of Computer Science and Information Engineering Penghu Taiwan

ISBN: (纸本)9798331530839

In offshore aquaculture operations, personnel equipped with diving gear are often necessary to inspect the underwater net cages for damage, particularly on the sea floor. This manual inspection process is time-consuming and complex. To overcome this problem, this paper proposes a computer vision solution for identifying damage in underwater net cages to address the inefficiencies and challenges of traditional manual inspections. The proposed scheme utilizes a high-performance multi-branch computational architecture designed based on ShuffleNet architecture to detect net cage damage more efficiently. Experimental results demonstrate that this work performs well on the ImageNet ILSVRC-2010 dataset and achieves an accuracy of 88.54% in underwater net damage detection. © 2024 IEEE.

关键词： Aquaculture

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RUL Estimation for Power Electronic Devices Using RNNs

RUL Estimation for Power Electronic Devices Using RNNs

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2024 Prognostics and System Health Management Conference, PHM 2024

作者： Cai, Congrui Lu, Zhonghai School of Electrical Engineering and Computer Science KTH Royal Institute of Technology Embedded Systems Master Program Sweden School of Electrical Engineering and Computer Science Department of Electrical Engineering KTH Royal Institute of Technology Sweden

ISBN: (纸本)9798350360585

Recurrent Neural Networks (RNNs) are commonly used in data-driven approaches to estimate the Remaining Useful Lifetime (RUL) of power electronic devices. RNNs are preferred because their intrinsic feedback mechanisms are better suited to model time-series data. However, the impact of RNN complexity on estimation accuracy is rarely discussed in the literature. This issue is important because choosing a lower-complexity model that delivers the same or similar performance as a higher-complexity model can increase implementation efficiency. In the paper, we use three RNN models, namely, the vanilla version, LSTM (Long Short Term Memory) and GRU (Gated Recurrent Unit) to conduct RUL estimation for power electronic devices. We use two accelerated aging datasets, one dataset targeting the package failure of MOSFETs, and the other dataset targeting package failure of power diodes. Our study shows that a lower-complexity RNN does not necessarily deliver a lower performance. Similarly, a higher-complexity model does not assure a higher performance. As such, our work highlights the importance of selecting a proper neural network for RUL estimation not biased towards complex models. This is especially useful and important for implementing such RUL estimation techniques in embedded resource-constrained and speed-limited computins platforms. © 2024 IEEE.

关键词： Long short-term memory

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Design of an Automatic Price Tag System for Web-Based Retail Business 9

Design of an Automatic Price Tag System for Web-Based Retail...

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9th International Conference on Information Management and Technology, ICIMTech 2024

作者： Liawatimena, Suryadiputra Gunawan, Devina Bina Nusantara University Automotive & Robotics Program Computer Engineering Department BINUS ASO School of Engineering Computer Science Deparment BINUS Graduate Program Master of Computer Science Jakarta11480 Indonesia Bina Nusantara University Automotive & Robotics Program Computer Engineering Department BINUS ASO School of Engineering Jakarta11480 Indonesia

ISBN: (纸本)9798350390025

Modern retail businesses face a significant challenge with the inefficiency of manually changing price labels on shelves. This manual process not only consumes valuable time and resources but also increases the likelihood of errors, leading to potential inaccuracies in pricing and a less streamlined shopping experience for customers. The proposed solution is the implementation of an Electronic Shelf Label (ESL) system that automatically displays the prices of goods on retail business shelves. This system connects a website (front end and back end) to e-paper via a Wi-Fi network and microcontroller, allowing retail business owners to update prices more easily. Additionally, buyers can search for desired items through the website. The results are the time to send data from the website to the e-paper, namely, to know the performance of the e-paper used. The average time required from 10 attempts to send data from the website to the e-paper is 20.935 seconds. Since the data is connected to the server and will be updated automatically, using this system will be more efficient than manually changing the price label, although it takes time to transfer data from the website to the e-paper. © 2024 IEEE.

关键词： Sales

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