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Cryptotanshinone Suppresses the STAT3/BCL-2 Pathway to Provoke Human Bladder Urothelial Carcinoma Cell Death

Environmental Toxicology 2025年第4期40卷 624-635页

作者： Tung, Min-Che Chang, Ge-Man Dai, Wen-Chyi Hsu, Chen-Hsuan Chang, Hsiang-Chun Yang, Wei-Ting Ho, Yann-Jen Lu, Chien-Hsing Chen, Yi-Hsin Chang, Chia-Che Division of Urology Department of Surgery Tungs' Taichung MetroHarbor Hospital Taichung Taiwan Graduate Institute of Biomedical Sciences National Chung Hsing University Taichung Taiwan Doctoral Program in Biotechnology Industrial Innovation and Management National Chung Hsing University Taichung Taiwan Department of Life Sciences National Chung Hsing University Taichung Taiwan Department of Obstetrics and Gynecology Taichung Veterans General Hospital Taichung Taiwan Doctoral Program in Translational Medicine National Chung Hsing University Taichung Taiwan Department of Nephrology Taichung Tzu Chi Hospital Buddhist Tzu chi Medical Foundation Taichung Taiwan School of Medicine Tzu Chi University Hualein Taiwan Department of Artificial Intelligence and Data Science National Chung Hsing University Taichung Taiwan Master Program in Precision Health Rong Hsing Research Center for Translational Medicine The iEGG and Animal Biotechnology Research Center National Chung Hsing University Taichung Taiwan Department of Medical Laboratory Science and Biotechnology Asia University Taichung Taiwan Department of Medical Research China Medical University Hospital Taichung Taiwan

Bladder cancer is one of the most common human malignancies worldwide. Aberrant activation of signal transducer and activator of transcription 3 (STAT3) is crucial to driving malignant progression and predicting poor prognosis of multiple human cancers, including bladder cancer, making STAT3 a promising target of cancer therapeutics. Cryptotanshinone (CTS) is an anticancer ingredient of Danshen (Salvia miltiorrhiza), a top-graded Chinese medicinal herb. However, whether CTS targets STAT3 to exert its cytotoxic effect on human bladder cancer remains unknown. Herein, we demonstrated that CTS is cytotoxic to multiple human urinary bladder transitional cell carcinoma (TCC) cell lines while sparing normal human urothelial cells. CTS provoked apoptosis-dependent bladder TCC cytotoxicity, as apoptosis blockage by z-VAD-fmk markedly rescued the clonogenicity of CTS-treated cells. Besides, CTS was found to suppress constitutive and interleukin 6-inducible activation of STAT3, evidenced by the downregulation of STAT3 tyrosine 705 phosphorylation and BCL2, a recognized STAT3 transcriptional target. Notably, ectopic expression of a dominant-active STAT3 mutant (STAT3-C) or BCL-2 alleviated CTS-induced apoptosis and clonogenicity inhibition, thus confirming STAT3 blockade as a pivotal mechanism of CTS's cytotoxic action on bladder TCC cells. Lastly, immunoblotting revealed that CTS lowered the levels of active JAK2, an upstream kinase that mediates STAT3 tyrosine 705 phosphorylation. Altogether, we conclude that the blockade of the JAK2/STAT3/BCL-2 antiapoptotic signaling axis is a vital mechanism whereby CTS provokes bladder cancer cytotoxicity. The current evidence implicates CTS's potential to be translated into a bladder cancer therapeutic agent. © 2024 Wiley Periodicals LLC.

关键词： Cell death

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Two Novel Compounds Isolated from the Marine Fungal Symbiont of Aspergillus unguis Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells: In vitro Study

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Journal of Experimental Pharmacology 2025年 17卷 37-50页

作者： Bashari, Muhammad Hasan Agung, Mochamad Untung Kurnia Ariyanto, Eko Fuji Al Muqarrabun, Laode Muhammad Ramadhan Salsabila, Syefira Chahyadi, Agus Rosandy, Andi Rifki Afifah, Ervi Afni, Merry Atmaja, Harold Eka Putri, Tenny Utami, Fitria Subhan, Beginer Syafrizayanti Andriani, Yosie Elfahmi Department of Biomedical Sciences Faculty of Medicine Universitas Padjadjaran West Java Bandung Indonesia Department of Marine Science Faculty of Fisheries and Marine Science Universitas Padjadjaran West Java Bandung Indonesia University Center of Excellence for Nutraceuticals Bioscience and Biotechnology Research Center Bandung Institute of Technology West Java Bandung Indonesia Graduate School of Master Program Faculty of Medicine Universitas Padjadjaran Bandung Indonesia Biomedical Laboratory Faculty of Medicine Universitas Padjadjaran West Java Bandung Indonesia Department of Marine Science and Technology Faculty of Fisheries and Marine Science IPB University West Java Bogor Indonesia Biochemistry Laboratory Department of Chemistry Universitas Andalas West Sumatera Padang Indonesia Institute of Climate Adaptation and Marine Biotechnology Universiti Malaysia Terengganu Kuala Nerus Terengganu Malaysia School of Pharmacy Bandung Institute of Technology West Java Bandung Indonesia

Purpose: A promising feature of marine sponges is the potential anticancer efficacy of their secondary metabolites. The objective of this study was to explore the anticancer activities of compounds from the fungal symbiont of Aaptos suberitoides on breast cancer cells. Methods: In the present research, Aspergillus unguis, an endophytic fungal strain derived from the marine sponge A. suberitoides was successfully isolated and characterized. Subsequently, ethyl acetate extraction and isolation of chemical constituents produced was performed. The structures of the isolated compounds were identified using several spectroscopic methods, ie, UV, NMR, and mass spectrometry. Thereafter, MDA-MB-231, MCF-7 breast cancer cells and HaCat cells were treated with the isolated compounds. Not only viability, apoptosis, and cell cycle analyses were conducted, but also the mRNA expression of MCL1, BCL2L1, AKT1 and CDK2 were evaluated. Results: The extract showed cytotoxic activity in breast cancer cells. Two novel compounds were successfully isolated and identified, ie, Unguisol A (15.1 mg) and Unguisol B (97.9 mg). Both compounds share the same basic skeleton and comprise an aromatic ring which is attached to a sulphur-containing, seven-membered ring via an oxygen atom. This marked the first-time isolation of Unguisol A and Unguisol B from A. unguis, highlighting their novelty. Both compounds induced early apoptosis (p < 0.01) and cell cycle arrest at the S phase (p < 0.05) in MDA-MB-231 cells, but not in HaCat cells. Both compounds suppressed BCL2L1 and AKT1 mRNA expression (p < 0.01). Conclusion: Two novel compounds were isolated from A. unguis. Unguisol A and Unguisol B induced apoptosis in MDA-MB-231 breast cancer cells via BCL2L1 mRNA downregulation, while both compounds induced cell cycle arrest at the S phase through AKT1 mRNA downregulation. © 2025 Bashari et al.

关键词： Aspergillus unguis drug screening assays Porifera triple negative breast neoplasms

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Efficient butanol production from sweet sorghum stem juice by a co-culture system at an optimum temperature: Insights from oxidation-reduction potential monitoring and pilot scale validation

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Carbon Resources Conversion 2025年第2期8卷

作者： Daengbussadee, Chalida Laopaiboon, Pattana Lay, Chyi-How Laopaiboon, Lakkana Khon Kaen University Khon Kaen40002 Thailand Department of Biotechnology Faculty of Technology Khon Kaen University Khon Kaen40002 Thailand Khon Kaen University Khon Kaen40002 Thailand Master's Program of Green Energy Science and Technology Feng Chia University Taichung40724 Taiwan General Education Center Feng Chia University Taichung40724 Taiwan

A two-stage co-culture approach was employed in an acetone-butanol-ethanol (ABE) fermentation. An obligate aerobic bacterium, Arthrobacter sp., was first grown for 6 h at 30 °C to create anaerobic conditions. Subsequently, Clostridium beijerinckii TISTR 1461 was inoculated and a fermentation was performed at 37 °C. To identify an intermediate temperature suitable for both microorganisms, their growth was examined at 30, 34, and 37 °C. C. beijerinckii exhibited the highest specific growth rate at 37 °C, while Arthrobacter sp. displayed similar growth rates at all tested temperatures. Butanol production from a synthetic medium (P2 medium) by C. beijerinckii at different temperatures using oxygen-free nitrogen (OFN) gas flushing as a control treatment revealed that fermentation at 37 °C gave the highest butanol concentration (PB, 9.98 g/L). Consequently, 37 °C was chosen for butanol production from sweet sorghum stem juice (SSJ) by co-culture of these two microorganisms in 1-L screw–capped bottles. Compared to the control treatment, higher PB (11.38 g/L), yield (YB/S, 0.37 g/g) and productivity (QB, 0.24 g/L·h) were achieved using the co-culture system. These results were further confirmed by monitoring the oxidation–reduction potential (ORP) during ABE fermentation in a 2-L stirred-tank bioreactor (STR). Moreover, when the co-culture fermentation at 37 °C was scaled up in a 30-L STR, the PB, YB/S and QB values were comparable to those obtained in the 2-L STR. Therefore, co-culture fermentation of Arthrobacter sp. and C. beijerinckii TISTR 1461 at 37 °C represents a promising method for large-scale butanol production. © 2024

关键词： Fruit juices

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Dadih, traditional fermented buffalo milk: a comprehensive review of the aspects of gastronomy, health benefits, and product development

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Journal of Ethnic Foods 2025年第1期12卷 1-17页

作者： Pramana, Angga Yudhistira, Bara Jayalaksamana, Maghfirah Kurnia, Dihan Husnayain, Naila Pramitasari, Rianita Department of Agricultural Technology Faculty of Agriculture Universitas Riau Kampus Bina Widya Km. 12.5 Simpang Baru Kota Pekanbaru Indonesia Department of Food Science and Technology Faculty of Agriculture Sebelas Maret University Jl. Ir. Sutami 36 A Kentingan Surakarta 57126 Indonesia Entrepreneurship Development Center (PPKwu) Sebelas Maret University Jl. Ir. Sutami 36 A Kentingan Surakarta 57126 Indonesia Department of Agro-Industrial Technology Faculty of Agricultural Technology Universitas Gadjah Mada Yogyakarta Indonesia Research Center for Biomass and Bioproducts National Research and Innovation Agency of Indonesia (BRIN) West Java Bogor 16911 Indonesia Politeknik Pertanian Negeri Payakumbuh Animal Production Harau Lima Puluh Kota 26271 Indonesia International Master Program of Agriculture National Chung Hsing University Taichung City 402 Taiwan Food Technology Study Program Faculty of Biotechnology Atma Jaya Catholic University of Indonesia BSD Campus Banten Tangerang 15345 Indonesia

Indonesia is a country with a rich array of traditional meals renowned for their health benefits and potential to serve as functional foods, including dadih. Several studies have shown that dadih is a traditional fermented product from Indonesia, with various culinary applications. In addition, it is typically produced from fermented buffalo milk, which forms an unchanged or broken lump when fermented at room temperature. This product is also widely consumed by the Minangkabau ethnic group in Central Sumatra (nowadays known as the provinces of West Sumatra, Riau, Kepulauan Riau, and Jambi), holding a significant position in their culinary practices. Dadih is often traditionally processed through spontaneous fermentation in bamboo containers without the need for additional inoculation with buffalo milk. The indigenous dadih lactic acid bacteria has been evaluated to have several advantages, including immunomodulatory, antioxidant, antimutagenic, hypocholesterolemic, and antimicrobial properties, by various mechanisms. In addition, this product offers significant nutritional content, promoting digestive health and enhancing the body’s resilience. © The Author(s) 2024.

关键词： Dadih Gastronomic Health benefit Indonesia Minangkabau

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The gut-skin axis in psoriasis: Evidence-based insights from a meta-analysis on probiotics-synbiotics-mediated microbiota interventions

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Medicine in Microecology 2025年 25卷

作者： Amalia, Nurlinah Wicaksono, Danar Wiyarta, Elvan Rampengan, Derren David Christian Homenta Darmawan, Hari Aditya, Muhammad Reva Athallah, Ariq Fadhil Cahyono, Maulana Bagus Adi Alatas, Aiman Idrus Tallei, Trina Ekawati Tjandrawinata, Raymond Rubianto Bhunia, Arun K. Nurkolis, Fahrul Medical Study Program Faculty of Medicine Universitas Brawijaya East Java Malang Indonesia Master Program of Biomedical Science Faculty of Medicine Universitas Brawijaya East Java Malang Indonesia Medical Research Center of Indonesia East Java Surabaya Indonesia Alumnus Department of Dermatology and Venereology Faculty of Medicine Public Health and Nursing Universitas Gadjah Mada Yogyakarta Indonesia University of Indonesia Hospital Depok 16424 Indonesia Risetku Jakarta South Jakarta 12820 Indonesia Medical Study Program Faculty of Medicine Universitas Sam Ratulangi North Sulawesi Manado Indonesia Dermatovenerology Department Faculty of Medicine Universitas Tarumanagara Jakarta Indonesia Medical Study Program Faculty of Medicine Universitas Airlangga East Java Surabaya Indonesia Program of Clinical Microbiology Residency Faculty of Medicine University of Indonesia Jakarta Indonesia Department of Biology Faculty of Mathematics and Natural Sciences Sam Ratulangi University Manado 95115 Indonesia Center for Pharmaceutical and Nutraceutical Research and Policy Faculty of Biotechnology Atma Jaya Catholic University of Indonesia Jakarta 12930 Indonesia Molecular Food Microbiology Laboratory Department of Food Science Purdue University West Lafayette 47907 IN United States Purdue Institute of Inflammation Immunology and Infectious Diseases Purdue University West Lafayette 47907 IN United States Department of Comparative Pathobiology Purdue University West Lafayette 47907 IN United States Master of Basic Medical Science Faculty of Medicine Universitas Airlangga Surabaya Indonesia State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga) Yogyakarta 55281 Indonesia

Psoriasis, a chronic immune-mediated skin condition, has been intricately linked to gut microbiota dysbiosis through the gut-skin axis. This meta-analysis synthesizes data from 15 randomized controlled trials encompassing 1,423 participants to evaluate the efficacy of gut microbiota interventions—probiotics and synbiotics—in psoriasis management. The findings reveal that probiotics significantly improved the Psoriasis Area and Severity Index (PASI) (Mean Difference [MD]: −4.05, 95 % CI: −6.73 to −1.38;p < 0.0001) and Dermatology Life Quality Index (DLQI) (MD: −5.74, 95 % CI: −11.45 to −0.03;p = 0.0001), outperforming synbiotics and systemic pharmacological therapies such as anti-TNF-α and anti-interleukin agents. Notably, probiotics demonstrated superior systemic anti-inflammatory (TNF-α and IL-17) and immunomodulatory responses, and enhanced gut barrier integrity. This study highlights probiotics as a promising adjunct or alternative therapy, paving the way for integrative treatment strategies that address psoriasis's multifaceted pathophysiology. Future research should focus on long-term efficacy and molecular mechanisms to optimize outcomes. These findings could redefine therapeutic paradigms, offering a cost-effective and accessible solution for millions of psoriasis patients worldwide. © 2025 The Authors

关键词： Gut microbiota Gut-skin axis Immune modulation Probiotics Psoriasis Synbiotics Systemic inflammation

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Development of BACE2-IN-1/tranylcypromine-based compounds to induce steroidogenesis-dependent neuroprotection

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Biomedicine and Pharmacotherapy 2025年 183卷 117851-117851页

作者： Banerjee, Suddhasatwa Hsu, Ying-Ting Nguyen, Duc-Hieu Yeh, Shiu-Hwa Liou, Ke-Chi Liu, Jr-Jiun Liou, Jing-Ping Chuang, Jian-Ying School of Pharmacy Taipei Medical University Taipei 11031 Taiwan Ph.D. Program in Medical Neuroscience Taipei Medical University and National Health Research Institutes Taipei 11031 Taiwan Institute of Biotechnology and Pharmaceutical Research National Health Research Institutes Miaoli County 35053 Taiwan TMU Research Center for Drug Discovery Taipei Medical University Taipei 11031 Taiwan Ph.D. Program in Drug Discovery and Development Industry College of Pharmacy Taipei Medical University Taipei 110 Taiwan International Master Program in Medical Neuroscience Taipei Medical University New Taipei City 23564 Taiwan TMU Research Center of Neuroscience Taipei Medical University Taipei 11031 Taiwan Department of Biomedical Science and Environmental Biology Kaohsiung Medical University Kaohsiung 80780 Taiwan

Traumatic brain injury (TBI) constitutes a significant burden on global healthcare systems, especially affecting younger populations, where it is a leading cause of disability and mortality. Current treatments for TBI mainly focus on preventing further brain damage and controlling symptoms. However, despite these approaches, several clinical needs remain unmet. Revelations from single-cell RNA sequencing (scRNA-seq) performed to determine cell-type heterogeneity and gene expression changes in brain tissue indicated that brain trauma increases the expression of lysine-specific demethylase 1 (LSD1) and secretase 2 (BACE2). To capitalize on this finding, a medicinal chemistry campaign was conducted to pragmatically insert tranylcypromine, an LSD1 inhibitor, into a carefully designed BACE2 inhibitory template (BACE2-IN-1). Additionally, tranylcypromine was structurally modified to enhance the effects of LSD1 inhibition in TBI. As a result, a tractable neuroprotective agent, BACE2-IN-1/tranylcypromine-based compound 4, was identified, showing potential to maintain Neuro-2a cell survival by alleviating mitochondrial damage after oxidative stress. Compound 4 also restored TBI-mediated inhibition of the cholesterol biosynthetic pathway (mevalonate pathway) and damage of redox metabolism, increasing neuroprotective effects. Furthermore, behavioral assays, including nest-building and cognitive performance tests, demonstrated significant improvement in mice post-TBI following treatment with compound 4. Taken together, the outcomes of this study validate the favorable effects of inhibiting LSD1 and beta-secretase in mitigating mitochondrial stress and promoting neurometabolic recovery in TBI. These findings pave the way for the development of rationally designed inhibitors as promising neuroprotective agents, potentially addressing unmet clinical needs in TBI treatment. © 2025 The Authors

关键词： Beta-secretase LSD1 Oxidative stress Sterol biosynthesis Tranylcypromine Traumatic brain injury

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Distinctive nitrogen oxides reduction capabilities of a Denitrifying Polyphosphate Accumulating Organisms (DPAOs) enrichment in a long-term nitrite fed sequencing batch reactor

Distinctive nitrogen oxides reduction capabilities of a Deni...

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Water Environment Federation Technical Exhibition and Conference 2017, WEFTEC 2017

作者： Gao, Han Zhao, Xiaotian Zhou, Lei Wells, George F. Department of Civil and Environmental Engineering Northwestern University EvanstonIL United States Master of Science in Biotechnology Program McCormick School of Engineering Northwestern University EvanstonIL United States

ISBN: (纸本)9781510863682

Denitrifying polyphosphate accumulating organisms (DPAOs), are capable of nitrate and/or nitrite (NO2-) reduction coupled to P uptake when subjected to alternating anaerobic/anoxic conditions. However, strong accumulation of nitrous oxide (N2O) has been previously observed in DPAO enrichments under high NO2- concentrations. To date, denitrification capability and denitrifying P uptake rates of DPAOs using different electron acceptors after long-term exposure to elevated NO2- concentration hasn't been examined. To address this knowledge gap, we operated a lab-scale SBR under alternating anaerobic/anoxic conditions with high nitrite feed for >2 years. Ex-situ batch assays were performed to clarify capacity for reduction of various nitrogen oxides and P uptake of the DPAOs enrichment culture. These batch assays revealed distinct nitrogen oxides reduction and denitrifying P uptake capabilities. High-throughput 16S rRNA sequencing combined with qPCR assays revealed the selection of putative DPAOs and other potential denitrifying microbial consortia responsible for N2O accumulation under high nitrite-feeding regimes. Copyright © 2017 Water Environment Federation.

关键词： Denitrification

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Converting hydroponic effluent into high PUFA-rich biomass by mixotrophically cultivated microalga Chlorella sp. through light regulation for sustainable production of bioingredient and bioenergy

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Journal of Environmental Chemical Engineering 2025年第3期13卷

作者： Nanthakrit Sriket Antira Wichaphian Apiwit Kamngoen Jeeraporn Pekkoh Wasu Pathom-aree Yupa Chromkaew Benjamas Cheirsilp Wageeporn Maneechote Yoong Kit Leong Sirasit Srinuanpan Master of Science Program in Applied Microbiology (International Program) Department of Biology Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Department of Biology Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Microbial Biorefinery and Biochemical Process Engineering Research Group Chiang Mai University Chiang Mai 50200 Thailand Center of Excellence in Microbial Diversity and Sustainable Utilization Chiang Mai University Chiang Mai 50200 Thailand Department of Plant and Soil Sciences Faculty of Agriculture Chiang Mai University Chiang Mai 50200 Thailand Program of Biotechnology Faculty of Agro-Industry Prince of Songkla University Hat Yai Songkhla 90110 Thailand Office of Research Administration Office of the University Chiang Mai University Chiang Mai 50200 Thailand Department of Chemical and Materials Engineering Tunghai University Taichung 407 Taiwan

Currently, addressing global environmental challenges—particularly the management of nutrients and resource recovery from hydroponic effluent (HE)—is crucial for promoting sustainable agriculture and fostering circularity in the pursuit of a net-zero future. This study aimed to evaluate the potential of Chlorella sp. to convert HE into high-value biomass rich in polyunsaturated fatty acids (PUFAs) under mixotrophic conditions with optimized light illumination. The results demonstrated that supplementing HE with half the concentration of commercial JM medium, and optimizing with blue light, a 16-hour light/8-hour dark photoperiod, and a light intensity of 50 µmol/m 2 /s resulted in a biomass production of 4.04 g/L, containing 16.81 % lipids and 34.04 % PUFAs. These conditions also achieved significant pollutant removal from HE, including 93.33 % nitrate reduction, 100 % ammonia removal, 99.08 % phosphate removal, and 92.68 % reduction in chemical oxygen demand. The nutritional profile of the fatty acids suggests potential applications in the functional ingredient sector, while their characteristics indicate strong potential as biodiesel feedstock, meeting international biodiesel fuel standards. Overall, this study presents an eco-friendly model for nutrient recovery from hydroponic effluent using Chlorella sp., promoting sustainable agriculture, reducing environmental impact, and contributing to bioresource innovation.

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High resolution melting real-time PCR detect and identify filarial parasites in domestic cats

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Asian Pacific Journal of Tropical Medicine 2018年第12期11卷 682-687页

作者： Darawan Nonsaithong Supit Yotmek Somsri Yotmek Hathai Nochote Sirichit Wongkamcha Sittiruk Roytrakul Usa Lek-Uthai Master of Science (Public Health) Program in Infectious Diseases and Epidemiology Faculty of Graduate Studies Mahidol University Department of Parasitology and Entomology Faculty of Public Health Mahidol University Center for Disease Control Vector Borne Disease Control Center 11.3 Surat Thani Province Department of Parasitology Faculty of Medicine Siriraj Hospital Mahidol University National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA)

Objective: To detect and identify filarial parasites in dried blood spots(DBS) collected from domestic cats using high resolution melting real-time PCR(HRM RT-PCR). Methods: A total of 208 DBS were collected from domestic cats in a brugian filariasis endemic areas in Surat Thani Province, southern Thailand. Microfilariae were found in 9 blood slides using Giemsa-stained thick blood film. The extracted DNA from blood spot volumes of 10 and 20 μL DBS with positive filarial parasites in cats were performed using HRM RT-PCR method. The primers were designed based on the partial mitochondrial 12S rRNA gene for identifying Brugia malayi, Brugia pahangi, Dirofilaria immitis. All purified samples were then detected. Results: Using different volumes of 10 μL and 20 μL DBS could easily distinguish filarial parasites and showed similar results. PCR amplicons of Brugia malayi, Brugia pahangi and Dirofilaria immitis were determined at melting peak(temperature) of 75.70℃, 77.46 ℃, and 73.56 ℃, respectively. All 9 positive DBS samples showed positive Brugia pahangi and similar nucleotide sequences. Conclusions: This HRM RT-PCR method is able to diagnose, identify and discriminate filarial parasites collected from DBS, which is simple and inexpensive compared with other probe-based genotyping methods. Furthermore, this method is useful to survey, prevent and control filariasis.

关键词： High resolution melting analysis Dried blood spot Brugia malayi Brugia pahangi Dirofilaria immitis

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Application of LDPE Film–Loaded Nutmeg Essential Oil Pickering Emulsion to Extend Tilapia Fillets’ Shelf Life

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Food and Bioprocess Technology 2024年第10期17卷 3031-3045页

作者： Yudhistira, Bara Chang, Chao-Kai Punthi, Fuangfah Cheng, Kuan-Chen Husnayain, Naila Hsieh, Chang-Wei Department of Food Science and Biotechnology National Chung Hsing University Taichung City402 Taiwan Department of Food Science and Technology Sebelas Maret University Surakarta City Central Java57126 Indonesia Institute of Biotechnology National Taiwan University Taipei10617 Taiwan Graduate Institute of Food Science Technology National Taiwan University Taipei10617 Taiwan Department of Optometry Asia University Taichung City413305 Taiwan Department of Medical Research China Medical University Hospital Taichung City404333 Taiwan International Master Program of Agriculture National Chung Hsing University Taichung City402 Taiwan

The food industry is still dependent on plastics for packaging materials, primarily due to their effectiveness in safeguarding products from external factors, such as moisture, light, and gas. However, traditional plastic packaging lacks the ability to actively preserve the quality and extend the shelf life of food. To address this challenge, various methods are currently being developed through surface modifications and the incorporation of active compounds. In most cases, studies on the modification of active film using low-pressure plasma (LPP) use different parameters, including time, pressure, and power, without a systematic optimization process. Therefore, this study aims to determine the optimum parameters for the combination of LPP treatment and Pickering emulsion (PE) of nutmeg essential oil (NEO) using Taguchi’s orthogonal assay design (OAD) to produce LDPE-active film. The results showed that the optimum conditions were 15 W LPP power, 30 s LPP duration time, and 1.5% PE-NEO concentration. These results showed a 50% improvement in power and time efficiency. LDPE film–loaded PE-NEO was then applied to tilapia fillets in cold storage. The results showed that the TBARS and TBVN values of the samples after the treatment were lower than those of the control by 12.11% and 16.74%, respectively. © The Author(s), under exclusive licence to Springer science+Business Media, LLC, part of Springer Nature 2024.

关键词： Cold storage

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