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Cytometry in the Short-Wave Infrared

ACS Nano 2024年第28期18卷 18534-18547页

作者： Liu, Te-I Wang, Jhih-Shan Nguyen, Ai-phuong Raabe, Marco Quiroz Reyes, Carlos Jose Lin, Chih-Hsin Lin, Ching-Wei Institute of Atomic and Molecular Sciences Academia Sinica Taipei City106319 Taiwan Department of Materials Science and Engineering National Taiwan University Taipei City106319 Taiwan Department of Physics University of Stuttgart Stuttgart70174 Germany Department of Chemistry National Tsing Hua University Hsinchu300044 Taiwan International Ph.D. Program in Biomedical Engineering Taipei Medical University New Taipei City235603 Taiwan Graduate Institute of Nanomedicine and Medical Engineering Taipei Medical University New Taipei City235603 Taiwan

Cytometry plays a crucial role in characterizing cell properties, but its restricted optical window (400-850 nm) limits the number of stained fluorophores that can be detected simultaneously and hampers the study and utilization of short-wave infrared (SWIR;900-1700 nm) fluorophores in cells. Here we introduce two SWIR-based methods to address these limitations: SWIR flow cytometry and SWIR image cytometry. We develop a quantification protocol for deducing cellular fluorophore mass. Both systems achieve a limit of detection of ∼0.1 fg cell-1 within a 30 min experimental time frame, using individualized, high-purity (6,5) single-wall carbon nanotubes as a model fluorophore and macrophage-like RAW264.7 as a model cell line. This high-sensitivity feature reveals that low-dose (6,5) serves as an antioxidant, and cell morphology and oxidative stress dose-dependently correlate with (6,5) uptake. Our SWIR cytometry holds immediate applicability for existing SWIR fluorophores and offers a solution to the issue of spectral overlapping in conventional cytometry. © 2024 The Authors. Published by American Chemical Society.

关键词： Oxygen

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Reusable Hydrogen Storage Tank with Flange design 10

Reusable Hydrogen Storage Tank with Flange Design

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10th International Conference on Applied System Innovation, ICASI 2024

作者： Li, Yun-Qi Chen, Hsuan-Jung Yang, Hsi-Wen Chen, Chih-Yuan Chang, Shao-Fu Chen, Ssu-Ying Chen, Chien-Chon College of Engineering and Science Ph.D. Program in Materials and Chemical Engineering National United University Miaoli Taiwan Michigan State University Department of Chemistry MI48824 United States National United University Department of Materials Engineering Miaoli Taiwan Graduate Institute of Intellectual Property National Taipei University of Technology Taiwan National United University Department of Energy Engineering Miaoli Taiwan

ISBN: (纸本)9798350394924

For how to store and transport hydrogen, the first thought is to store hydrogen directly in the cylinder. However, this method is not desirable, because generally at least 700 atm high pressure, the hydrogen contained in the cylinder to be useful. Therefore it is very unsafe at such high pressures. In This paper, we design and production of a new generation of solid-state hydrogen storage and discharge container. The goal of 'development of solid-state hydrogen storage technology' is to break through the bottlenecks of hydrogen storage technology safety, hydrogen storage density, high-efficiency hydrogen absorption and release, transportation safety, and engineering practice. © 2024 IEEE.

关键词： Aluminum

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Ethylene Glycol-Manipulated Syntheses of Calcium Carbonate Particles and dNA Capsules toward Efficient ATP-Responsive Cargo Release

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ACS Applied Bio materials 2023年第8期6卷 3351-3360页

作者： Lin, Yu-Hsuan Singuru, Murali Mohana Rao Marpaung, david Septian Sumanto Liao, Wei-Ching Chuang, Min-Chieh Department of Chemistry Tunghai University Taichung407224 Taiwan International Ph.D. Program in Biomedical and Materials Science Tunghai University Taichung407224 Taiwan Department of Biosystems Engineering Institut Teknologi Sumatera Lampung Selatan35365 Indonesia Institute of Biochemistry and Molecular Biology National Yang Ming Chiao Tung University Taipei112304 Taiwan

Cargo molecule-encapsulated dNA capsules synthesized with a solid sacrificial template have elicited significant interest in the last decade and have been used for active materials in applications ranging from biosensors to drug delivery. However, the correlation between template properties and the subsequent assembly and triggered release behavior of the resultant carriers remain uninvestigated. In this study, ethylene glycol (EG) was added during the CaCO3 precipitation synthesis to yield particles of various sizes and surface properties, and the adenosine triphosphate (ATP)-responsive release characteristics of the fabricated dNA capsules affected by these particle properties were investigated. The geometry, crystallization, and surface morphology of the CaCO3 particles co-precipitated at various EG concentrations were characterized. We discuss the integrity of cross-linking hybridization, fluorescent molecule internalization, degree of leakage, and release efficiency of the resulting dNA capsules and their relevance brought by particle properties. To achieve efficient encapsulation and cargo release, the surface roughness of the CaCO3 particles was explored and was deemed a key determinant of the compactness of the dNA shell after template removal. This effect was particularly strong in CaCO3 particles in connection with high EG concentrations. The dNA capsules fabricated using 83% EG exhibited low leakage, high loading, and moderate release efficiencies as well as a greater apparent association constant with ATP due to their small particle size and the high-integrity dNA shells. © 2023 American Chemical Society.

关键词： Ethylene glycol

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An Improvement of Three-Factor Remote User Authentication Protocol Using ECC

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International Journal of Network Security 2024年第3期26卷 528-534页

作者： Hwang, Min-Shiang Lin, Cheng-Ying Wu, Chia-Chun Department of Computer Science & Information Engineering Asia University Taichung Taiwan 500 Lioufeng Rd. Wufeng Taichung Taichung 41354 Taiwan Department of Medical Research China Medical University Hospital China Medical University Taiwan Ph.D. Program in Artificial Intelligence Asia University Taichung Taiwan 500 Lioufeng Rd. Wufeng Taichung Taichung 41354 Taiwan Kinmen892 Taiwan

With the development of the Internet, many patients’ private medical data are maliciously stolen by attackers. Therefore, Liu et al. proposed an improved ECC-based three-factor user authentication scheme. The main advantage of their scheme is that it provides the same or better security than other schemes using more secondary keys. However, this article will show that Liu et al.’s scheme is vulnerable to denial of service attacks. In addition, their protocol uses public key cryptography, which is inefficient. Finally, the method is not immune to counterfeit smart card attacks. In this paper, we also improve their protocol from the perspective of practicality and security. © (2024), (International Journal of Network Security). All rights reserved.

关键词： Authentication

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Metacognitive-Based Collaborative programming: A Novel Approach to Enhance Learning Performance in programming Courses 6th

Metacognitive-Based Collaborative Programming: A Novel Appro...

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Proceedings of the 6th International Conference on Innovative Technologies and Learning, ICITL 2023

作者： Li, Wei Tseng, Judy C. R. Cheng, Li-Chen STEM Education Research Center Wenzhou University Zhejiang Province Wenzhou China Ph.D. Program in Engineering Science Chung Hua University Hsinchu Taiwan Department of Computer Science and Information Engineering Chung Hua University Hsinchu Taiwan Department of Information and Finance Management National Taipei University of Technology Taipei City Taiwan

ISBN: (纸本)9783031401121

Students’ computational thinking and programming skills may grow due to collaborative programming. But as the researchers have noted, students frequently do not use metacognition to manage their cognitive activities while collaborating, which negatively affects learning. This study created a metacognition-based collaborative programming (MCP) system to improve students’ performance in collaborative programming. A seven-week study examined how the approach affected students’ performance in programming courses. The 88 middle school students were split into two groups: the experimental group received the metacognition-based collaborative programming approach, and the control group received the conventional computer-supported collaborative programming approach. The results indicated that the metacognitive-based collaborative programming approach enhanced students’ academic scores in programming courses and their computational thinking tendencies. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

关键词： Learning systems

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Integration of a Microfluidics System With a U-Shaped Optical Fiber Sensor for Sensing Various Concentrations of Glucose

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IEEE Sensors Journal 2024年第13期24卷 20617-20628页

作者： Hsiang-Cheng Hsu Tien-Bao Tran Thi Thuy-Vy Nguyen Hoang-dang Le Chia-Chin Chiang Department of Ph.D. Program in Maritime Science and Technology College of Maritime National Kaohsiung University of Science and Technology Kaohsiung Taiwan Department of Mechanical Engineering National Kaohsiung University of Science and Technology Kaohsiung Taiwan College of Engineering Can Tho University Ninh Kieu Can Tho Vietnam

This study introduces a successfully manufactured U-shaped optical fiber sensor integrated with a microfluidics system to detect varying glucose concentrations. It outlines the fabrication process of the microfluidics system, accomplished using a CO2 laser on polymethyl methacrylate (PMMA) material. Through three cycles of experiments involving different glucose concentrations, the results indicate a consistent trend in both resonance wavelength and transmission loss, showing an increase as glucose concentration rises from 5% to 50%. The system demonstrates a high wavelength sensitivity coefficient of 0.114 nm/% with ${R}^{{2}} =0.95$ and a notable transmission loss sensitivity of 0.09 dB/% and ${R}^{{2}} =0.94$ . This advanced system proves to be effective in achieving rapid, sensitive, and cost-efficient glucose monitoring. Consequently, the microfluidics system is deemed suitable for its intended purpose.

关键词： Microfluidics Glucose Optical fiber sensors Sensors Sensitivity Claddings Viscosity

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Enhancement of piezophototronic hydrogen evolution reaction through the polarization of ferroelectric Bi4Ti3O12 microplates

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International Journal of Hydrogen Energy 2024年第PartB期50卷 15-25页

作者： Chang, Shun Cheng Chen, Po-Han Chen, Yu-Ching Wu, Jyh Ming Department of Materials Science and Engineering National Tsing Hua University 101 Section 2 Kuang Fu Road Hsinchu300 Taiwan High Entropy Materials Center National Tsing Hua University Section 2 Kuang Fu Road 101 Hsinchu300 Taiwan Ph.D. Program in Prospective Functional Materials Industry National Tsing Hua University 101 Section 2 Kuang Fu Road Hsinchu300 Taiwan

In this study, we analyzed the properties of Bi4Ti3O12 microplates in the context of hydrogen evolution reaction. Piezoresponse force microscopy revealed a typical butterfly curve, indicating that as-synthesized Bi4Ti3O12 exhibits ferroelectric properties. Under ultrasonication, the amount of hydrogen produced by polarized (2000 V) Bi4Ti3O12 increased from 728 to 1349 μmol g−1, which was 1.85 times higher than that generated by unpolarized Bi4Ti3O12. The increase in hydrogen evolution rate may be attributed to the gradual alignment of polarization within the ferroelectric domains of Bi4Ti3O12. Moreover, with synergistic piezophototronic activity, the amount of hydrogen produced by Bi4Ti3O12 was further increased to 1478 μmol g−1, which was 200% higher than that generated by unpolarized Bi4Ti3O12. Theoretical calculations revealed that Bi4Ti3O12 microplates exhibit a high piezoelectric potential along the x-axis, which is consistent with the spontaneous polarization of the microplates. By applying an external polarization field, ferroelectric polarization was activated to effectively separate free charges and extend carrier lifetime (from 0.6 to 0.84 ns). The application of an external electric field to ferroelectric Bi4Ti3O12 appears to be a favorable strategy for enhancing its piezophototronic activity. Our findings may facilitate future studies in the fields of environmental science and renewable energy. © 2023 Hydrogen Energy Publications LLC

关键词： Bismuth compounds

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Synergistic sodium alginate- and biochar-immobilized cells for enhancing fermentative hydrogen production from food waste

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Environmental science and Pollution Research 2024年第56期31卷 64524-64533页

作者： Ngamnurak, phonsini Plangklang, Pensri Pomdaeng, Prakaidao Ko, Ting-Wu Reungsang, Alissara Chu, Chen-Yeon Graduate School Khon Kaen University Khon Kaen40002 Thailand Department of Biology Technology Faculty of Technology Khon Kaen University Khon Kaen40002 Thailand Research Group for Development of Microbial Hydrogen Production Process From Biomass Khon Kaen University Khon Kaen40002 Thailand Institute of Green Products Feng Chia University Taichung Taiwan Ph.D. Program of Mechanical and Aeronautical Engineering Feng Chia University Taichung Taiwan Master’s Program of Green Energy Science and Technology Feng Chia University Taichung Taiwan Academy of Science Royal Society of Thailand Bangkok10300 Thailand

An immobilized hydrogen-producing consortium investigated biohydrogen production from food waste using a combination of sodium alginate and cassava rhizome biochar. We investigated the effect of varying the biochar concentration from 0 to 3% and the size of immobilized cells from 1 to 7 mm. Immobilized cells were prepared using 50% (v/v) enriched hydrogen-producing consortium, 2% (w/v) sodium alginate, and 0 to 3% (w/v) cassava rhizome biochar. The optimal conditions for achieving the highest hydrogen production in the batch fermentation reactor were identified as a biochar concentration of 2% (w/v) and an immobilized cell size of 2 mm. The highest hydrogen yield, maximum hydrogen production rate, and lag time recorded were 0.69 mmol H2/g-COd, 0.02 mmol H2/g-COd.h, and 41.51 h, respectively. This research highlights the potential of cassava biochar technology for efficient biohydrogen production from food waste, contributing to renewable energy generation and sustainable waste management. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.

关键词： Anaerobic digestion

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TiO2 Hole Transport Layer Incorporated in a Thermally Evaporated Sb2Se3 photoelectrode Exhibiting Low Onset Potential for photoelectrochemical Applications

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Energy and Fuels 2024年第17期38卷 16936-16948页

作者： Xavier, Thatheyus Peter Piraviperumal, Malar Kuo, Chih-Yu Govindasamy, Mani Thinfilm Photovoltaics Laboratory Department of Physics and Nanotechnology SRM Institute of Science and Technology Tamil Nadu Kattankulathur603203 India Department of Chemical Engineering & Biotechnology National Taipei University of Technology Taipei City10608 Taiwan Research Center for Intelligence Medical Devices Ming Chi University of Technology New Taipei City243303 Taiwan Department of Research and Innovation Saveetha School of Engineering SIMATS Chennai602105 India Taipei10608 Taiwan International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices Ming Chi University of Technology New Taipei City243303 Taiwan

developing cost-effective photoelectrodes with a low band gap in the NIR-visible regions remains a challenge to achieve effective hydrogen production. We report the details of superstrate-configured photocathodes, consisting of antimony selenide (Sb2Se3) light absorbing layers with titanium dioxide (TiO2) as a hole transport layer, exhibiting the capability for hydrogen evolution with broad range light absorption enabled by the low band gap value of ∼1.2 eV of Sb2Se3. Strong anisotropy is a significant characteristic property of Sb2Se3, allowing superior electrical transportation along the [hkl] crystallographic orientation, which is obtained via close space evaporation of presynthesized bulk material at a growth temperature of 250 °C, over the solution-processed TiO2 film on a fluorine-doped tin oxide (FTO) substrate. The superstrate-configured FTO/TiO2/Sb2Se3 photocathode exhibited a photocurrent density of 100 μA cm-2 at −0.3 V vs RHE in 0.5 M H2SO4 under 1 sun illumination. To further improve photocurrent density, Pt as a cocatalyst was deposited over Sb2Se3 that led to enhanced photocurrent to 3 mA cm-2 at −0.3 V vs RHE in 0.5 M H2SO4 under 1 sun illumination. despite the relatively lower photocurrent observed, which resulted due to a large conduction band offset (ΔECB = 0.99 eV) of the TiO2/Sb2Se3 interface in a superstrate configuration, a significantly lower onset potential of −0.1 V vs RHE was observed. The results are encouraging due to the low band gap of Sb2Se3, photocorrosion stability, and straightforward fabrication, which is expected to assist for significant outcomes. © 2024 American Chemical Society.

关键词： Titanium dioxide

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Analysis of Gait data Based on deep Learning to Predict Multiple Balance Scale Scores

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IEEE Sensors Journal 2024年第1期24卷 920-930页

作者： Lin, Chueh-Ho Peng, Chih-Wei Lee, I-Jung Wu, Chi-Ming Lin, Bor-Shing Taipei Medical University International Ph.D. Program in Gerontology and Long-Term Care Taipei City110 Taiwan Taipei Medical University Research Center in Nursing Clinical Practice Wan Fang Hospital Taipei City110 Taiwan Taipei Medical University School of Biomedical Engineering College of Biomedical Engineering Taipei City110 Taiwan Taipei Medical University School of Gerontology and Long-Term Care College of Nursing Taipei City110 Taiwan National Taipei University Department of Computer Science and Information Engineering New Taipei City237 Taiwan National Taipei University College of Electrical Engineering and Computer Science New Taipei City237 Taiwan

Unbalanced walking is increasingly common among older adults;therefore, routinely assessing the balance of older adults is crucial. The traditional method of assessing balance uses scales, requires the supervision of a physical therapist (PT), and is time-consuming. The present study, therefore, proposes a deep learning (dL) model architecture that combines a convolutional neural network (CNN) and a long short-term memory (LSTM) network model to predict the scores of three scales, namely the Berg balance scale (BBS), timed up and go (TUG), and single-leg stance (SLS). The gait data of 15 m of walking were collected from seven inertial measurement units (IMUs) and input into the CNN-LSTM model for evaluation. The BBS and TUG only require the participants to wear two IMUs on the left and right thighs, respectively, for accurate predictions. The mean absolute errors (MAEs) of predicting the scores of the BBS and TUG are 1.2562 and 1.4016 s, respectively;however, the MAE of score predictions of SLS is higher than that of the BBS and TUG, indicating that gait data cannot be used for assessing SLS;moreover, participants only wear one IMU on the right calf for BBS and TUG evaluations, which yield MAEs of 1.4334 and 1.5229 s, respectively. The proposed system can quickly and accurately predict the scores of the BBS and TUG. The proposed model can assist PTs with making clinical decisions. © 2001-2012 IEEE.

关键词： Long short-term memory

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