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High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH

Cell 2025年第12期188卷 3310-3328.e27页

作者： Zhu, Yanyu Balaji, Ashwin Han, Mengting Andronov, Leonid Roy, Anish R. Wei, Zheng Chen, Crystal Miles, Leanne Cai, Sa Gu, Zhengxi Tse, Ariana Yu, Betty Chentzu Uenaka, Takeshi Lin, Xueqiu Spakowitz, Andrew J. Moerner, W.E. Qi, Lei S. Department of Bioengineering Stanford University Stanford 94305 CA United States Department of Chemistry Stanford University Stanford 94305 CA United States Biophysics PhD Program Stanford University Stanford 94305 CA United States Computational Biology Program Public Health Sciences Division and Translational Data Science IRC Fred Hutchinson Cancer Center Seattle 98109 WA United States Department of Chemical Engineering Stanford University Stanford 94305 CA United States Department of Materials Science and Engineering Stanford University Stanford 94305 CA United States Institute for Stem Cell Biology & Regenerative Medicine and Department of Pathology Stanford University School of Medicine Stanford 94305 CA United States Sarafan ChEM-H Stanford University Stanford 94305 CA United States Chan Zuckerberg Biohub San Francisco 94080 CA United States

Three-dimensional (3D) genome dynamics are crucial for cellular functions and disease. However, real-time, live-cell DNA visualization remains challenging, as existing methods are often confined to repetitive regions, suffer from low resolution, or require complex genome engineering. Here, we present Oligo-LiveFISH, a high-resolution, reagent-based platform for dynamically tracking non-repetitive genomic loci in diverse cell types, including primary cells. Oligo-LiveFISH utilizes fluorescent guide RNA (gRNA) oligo pools generated by computational design, in vitro transcription, and chemical labeling, delivered as ribonucleoproteins. Utilizing machine learning, we characterized the impact of gRNA design and chromatin features on imaging efficiency. Multi-color Oligo-LiveFISH achieved 20-nm spatial resolution and 50-ms temporal resolution in 3D, capturing real-time enhancer and promoter dynamics. Our measurements and dynamic modeling revealed two distinct modes of chromatin communication, and active transcription slows enhancer-promoter dynamics at endogenous genes like FOS. Oligo-LiveFISH offers a versatile platform for studying 3D genome dynamics and their links to cellular processes and disease. © 2025 Elsevier Inc.

关键词： CRISPR imaging DNA communication dynamic tracking enhancer-promoter interaction high-resolution imaging live DNA imaging non-repetitive genome imaging Oligo-LiveFISH polymer modeling primary cell

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Compressed air energy storage systems (CAES) and assessment of expansion cooling as an ancillary process

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science Talks 2024年 10卷

作者： Abdul Hai Alami Ahmad Yasin Siren Khuri Ayman Mudallal Mohamad Ayoub Adnan Alashkar Rashid AlRashid Shamma Abdalla Sustainable and Renewable Energy Engineering Department University of Sharjah Sharjah P.O. Box 27272 United Arab Emirates Materials Science and Engineering PhD Program American University of Sharjah Sharjah P.O. Box 26666 United Arab Emirates Mechanical Engineering Department American University of Sharjah Sharjah 26666 United Arab Emirates

This work introduces compressed air energy storage (CAES) systems and their role in mitigating the lag between energy supply and demand. The concept of energy storage relies on storing energy during periods of low demand to supply it during periods of high demand, where CAES stands as a scalable solution with the ability to house ancillary systems in order to harvest useful effects such as expansion cooling. CAES on its own compares well with electrochemical batteries, while bearing a simpler system design and less stringent operational requirements; however, with the integration of thermal management the role of CAES can be extended to produce chilled water that can be used for cooling applications. Not only does expansion cooling broaden the utilization of CAES, it has also enhanced the energetic efficiency of the system by 4%. Future work within the scope of CAES includes the integration of heat pipes and latent heat energy storage, and experimenting with different working gases.

关键词： Compressed air energy storage (CAES) Mechanical energy storage Expansion cooling Thermal management

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Progress in experimental assessments of buoyancy work energy storage

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science Talks 2024年 10卷

作者： Abdul Hai Alami Mohamad Ayoub Adnan Alashkar Shamma Abdalla Ahmad Yasin Ayman Mdallal Sustainable and Renewable Energy Engineering Department University of Sharjah Sharjah P.O. Box 27272 United Arab Emirates Materials Science and Engineering PhD Program American University of Sharjah Sharjah P.O. Box 26666 United Arab Emirates Mechanical Engineering Department American University of Sharjah Sharjah 26666 United Arab Emirates

This work reiterates the potential of buoyancy work energy storage (BWES) systems which has been presented in previously published experimental-based literature. The concept of buoyancy work can be grasped when studying the operation of mobility devices such as hot air balloons or boats, where buoyancy plays a key role in keeping them afloat. Conventional experimental BWES systems that were studied in previous literature consist of a water tank, a submergible buoy, a motor/generator carrying out the charging/discharging processes, respectively, and a data acquisition interface. It is interesting to see the progression of this technology at lab scale, where initial designs included the use of a barrel as the water tank, and Styrofoam cut-outs as buoys. One design utilized a ring with multiple Styrofoam buoys attached to it, and it was later enhanced by adding helical engravings to the buoys' surface to affect its interaction with water positively during descension and ascension. The latest study added to this timeline of ongoing development investigates the effect of different buoy materials, surface coatings, buoy infill gasses, and load.

关键词： Buoyancy work energy storage Mechanical energy storage Offshore wind turbines Battery alternatives

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Emergence of Non-photoresponsive Catalytic Techniques for Environmental Remediation and Energy Generation

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Chemistry - An Asian Journal 2023年第9期18卷 e202300090页

作者： Kumar Sharma, Manish Khan, Imran Kaswan, Kuldeep Roy Barman, Snigdha Saha, Subhajit Hsieh, Wu-Chiao Chueh, Yu-Lun Wang, Yu-Lin Lee, Sangmin Choi, Dukhyun Lin, Zong-Hong Department of Materials Science and Engineering National Tsing Hua University Hsinchu30013 Taiwan Institute of NanoEngineering and Microsystems National Tsing Hua University Hsinchu30013 Taiwan International Intercollegiate PhD Program National Tsing Hua University Hsinchu30013 Taiwan Department of Renewable Energy Maulana Abul Kalam Azad University of Technology West Bengal741249 India Department of Obstetrics and Gynecology Chang Gung Memorial Hospital and College of Medicine Chang Gung University Taoyuan333 Taiwan Frontier Research Center on Fundamental and Applied Sciences of Matters National Tsing Hua University Hsinchu30013 Taiwan School of Mechanical Engineering Chung-Ang University Seoul06974 Korea Republic of Department of Mechanical Engineering Sungkyunkwan University Suwon16419 Korea Republic of Department of Biomedical Engineering National Taiwan University Taipei10617 Taiwan

Catalysis plays a crucial role in all the major applications and challenges in the environment, including energy generation and environmental remediation. Although photocatalysts and electrocatalysts are useful in addressing energy and environmental issues, they have some major drawbacks, such as low efficiency and easy charge recombination which limits their applications. Hence, it is imperative to design and explore new catalytic techniques that include non-photoresponsive catalysts. In this review, the detailed possibilities, characteristics and prospects of non-photoresponsive catalysts, such as piezocatalysts, thermocatalysts, pyrocatalysts, and tribocatalysts along with hybrid catalysts are described. The overall mechanism of each catalytic technique and its applications in different fields such as energy generation, environmental remediation, and carbon dioxide reduction are discussed. © 2023 Wiley-VCH GmbH.

关键词： Electrocatalysts

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Corrigendum to "Curcumin-laden dual-targeting fucoidan/chitosan nanocarriers for inhibiting brain inflammation via intranasal delivery" [Int. J. Biol. Macromol. 181 (2021) 835-846]

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International journal of biological macromolecules 2022年 206卷 1040-1041页

作者： Trong-Ming Don Wan-Ju Chang Pei-Ru Jheng Yi-Cheng Huang Er-Yuan Chuang Department of Chemical and Materials Engineering Tamkang University New Taipei City Taiwan College of Life Sciences Department of Food Science National Taiwan Ocean University Keelung Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering International PhD Program in Biomedical Engineering Graduate Institute of Biomedical Optomechatronics School of Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei Taiwan Research Center of Biomedical Device Taipei Medical University Taipei Taiwan Cell Physiology and Molecular Image Research Center Taipei Medical University-Wan Fang Hospital Taipei Taiwan. Cell Physiology and Molecular Image Research Center Taipei Medical University-Wan Fang Hospital Taipei Taiwan. Electronic address: ychuang@mail.ntou.edu.tw. Cell Physiology and Molecular Image Research Center Taipei Medical University-Wan Fang Hospital Taipei Taiwan. Electronic address: eychuang@tmu.edu.tw.

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Assessment of circulating extracellular vesicles from calorie-restricted mice and humans in ischaemic injury models

Journal of Extracellular Biology

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Journal of Extracellular Biology 2023年第5期2卷 e86页

作者： Jaimes, Manuel S. V. Liao, Chia-Te Chen, Max M. Czosseck, Andreas Lee, Tsung-Lin Chou, Yu-Hsiang Chen, Yung-Ming Lin, Shuei-Liong Lai, James J. Lundy, David J. Graduate Institute of Biomedical Materials & Tissue Engineering Taipei Medical University Taipei Taiwan Division of Nephrology Department of Internal Medicine Shuang Ho Hospital Taipei Medical University New Taipei Taiwan Division of Nephrology Department of Internal Medicine School of Medicine College of Medicine Taipei Medical University Taipei Taiwan TMU Research Center of Urology and Kidney (TMU-RCUK) Taipei Medical University Taipei Taiwan Division of Nephrology Department of Internal Medicine National Taiwan University Hospital College of Medicine National Taiwan University Taipei Taiwan Graduate Institute of Physiology College of Medicine National Taiwan University Taipei Taiwan Department of Bioengineering University of Washington Seattle WA United States Department of Materials Science and Engineering National Taiwan University of Science and Technology Taipei Taiwan International PhD Program in Biomedical Engineering Taipei Medical University Taipei Taiwan Center for Cell Therapy Taipei Medical University Hospital Taipei Taiwan

Calorie restriction (CR) and fasting affect lifespan, disease susceptibility and response to acute injury across multiple animal models, including ischaemic injuries such as myocardial infarction or kidney hypoxia. The cargo and function of circulating extracellular vesicles (EV) respond to changes in host physiology, including exercise, injury, and other interventions. Thus, we hypothesised that EVs induced following CR may reflect some of the beneficial properties of CR itself. In a pilot study, EVs were isolated from mice following 21 days of 30 % CR, and from eight human donors after 72 h water-only fasting. EV size, concentration and morphology were profiled by NTA, western blot and cryoEM, and their function was assessed using multiple assays related to ischaemic diseases. We found that EVs from post-fasting samples better protected cardiac cells from hypoxia/reperfusion (H/R) injury compared to pre-fasting EVs. However, there was no difference when used to treat H/R-injured kidney epithelial cells. Post-fasting derived EVs slowed the rate of fibroblast migration and slightly reduced macrophage inflammatory gene expression compared to pre-fasting derived EVs. Lastly, we compared miRNA cargos of pre- and post-fasting human serum EVs and found significant changes in a small number of miRNAs. We conclude that fasting appears to influence EV cargo and function, with varied effects worthy of further exploration. © 2023 The Authors. Journal of Extracellular Biology published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.

关键词： calorie restriction cardiomyocyte diet extracellular vesicle fasting hypoxia ketone macrophage microRNA serum

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Sustainable flower-like Cu-Ni metal-organic frameworks catalyst for enhanced electrochemical sensing and photocatalytic degradation of antibiotic pollutants

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Microchemical Journal 2025年 214卷

作者： N.S.K. Gowthaman Ameer Farithkhan E. Narayanamoorthi Ponnusamy Arul Chinnathambi Nandhini Vellaichamy Balakumar S. Selvarajan School of Engineering Monash University Bandar Sunway Selangor 47500 Malaysia Center for Advanced Energy Materials SRM TRP Engineering College Tiruchirapalli 621 105 Tamil Nadu India Center for Research Easwari Engineering College Chennai 600 089 Tamil Nadu India School of Materials Science and Engineering Pusan National University Busan 46241 Republic of Korea International PhD Program in Innovative Technology of Biomedical Engineering and Medical Devices Ming Chi University of Technology New Taipei City 243303 Taiwan Institute of Biochemical and Biomedical Engineering Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei 106 Taiwan Department of Materials Engineering Ming Chi University of Technology New Taipei City 24303 Taiwan Department of Chemistry Sri Ramakrishna College of Arts & Science Coimbatore 641 006 Tamil Nadu India Department of Chemistry Theni Kammavar Sangam College of Arts & Science Theni 625 531 Tamil Nadu India

Massive usage of antibiotics harms the environment through animals’ excrement and squeezes open-wellbeing issues. Enrofloxacin (EF), an antimicrobial agent, treats bacterial diseases and infections in cattle, poultry and exotic animals and exhibits harmful side effects. The present work proposed a sensitive and rapid electrochemical scaffold based on the Cu-Ni bimetallic-organic framework (Cu-Ni-MOF) for the detection of harmful EF in real-time applications. The Cu-Ni-MOF was synthesized through solvothermal approach with the support of a multifunctional organic ligand, 4,4-biphenyldicarboxylicacid and characterized through various analytical tools. The electrochemical sensor for EF detection was constructed by drop-casting the Cu-Ni-MOF on a glassy carbon electrode and the fabricated sensor exhibited higher catalytic activity when compared to the parental counterparts by exhibiting 1.7-fold higher current for EF oxidation. Further, the fabricated sensor is highly active in the linear range response of 200 nM–2 mM with a limit of detection of 15.17 nM and sensitivity of 638.71 µA mM cm −2 under optimized environments. The higher electrocatalytic activity of the Cu-Ni-MOF electrode synergistically stems from the strong interaction between Cu 2+ of the MOF and pyridone and carboxylate moieties of the EF and facile electron transition facilitated by Ni 2+ . The practicability of the sensor was evaluated in milk, egg and river water samples and the obtained results were validated with Liquid Chromatography-Mass Spectrometric technique. Furthermore, the prepared catalyst was utilized for the photochemical decontamination of EF and the present Cu-Ni-MOF catalyst delivered excellent photocatalytic activity in the decontamination of EF under visible light at ambient temperature.

关键词： Cu-Ni-MOF Electrochemical sensor Enrofloxacin Environmental pollutants Metal-organic framework Photocatalytic degradation

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Influence of compatibilizer and carbon nanotubes on mechanical, electrical, and barrier properties of PTT/ABS blends

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Advanced Industrial and engineering Polymer Research 2019年第3期2卷 121-125页

作者： Braga, Natália F. LaChance, Anna Marie Liu, Bin Sun, Luyi Passador, Fabio R. Technology Laboratory of Polymers and Biopolymers Federal University of São Paulo São José dos CamposSP12231-280 Brazil Polymer Program Institute of Materials Science University of Connecticut StorrsCT06269 United States Department of Chemical and Biomolecular Engineering University of Connecticut StorrsCT06269 United States Beijing Key Laboratory of Electrochemical Process and Technology for Materials College of Materials Science and Engineering Beijing University of Chemical Technology Beijing100029 China Department of Biomedical Engineering University of Connecticut StorrsCT06269 United States

Composites based on poly (trimethylene terephthalate) (PTT)/acrylonitrile butadiene styrene (ABS) blend with maleic anhydride grafted PTT (PTT-g-MA) as a compatibilizer and carbon nanotubes (CNTs) as reinforcers were prepared via melt-mixing, and the mechanical, electrical, and barrier properties of the as-fabricated composites were evaluated. Scanning electron microscopy (SEM) characterization of the cross-section of the composites after tensile test showed improved phase stability in the presence of the compatibilizer PTT-g-MA. The toughness of the composites was also improved with the addition of the compatibilizer and CNTs. Electrical conductivity tests were carried out and the results showed that CNT-reinforced composite exhibited significantly increased conductivity. Since barrier properties are very sensitive to structural changes, the oxygen transmission rate (OTR) of the composites was measured and the results showed that the composites possess a much high oxygen barrier compared to that of the neat PTT or ABS. © 2019 Kingfa SCI. & TECH. CO., LTD.

关键词： Carbon nanotubes

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CCL5 Protects Cortical Neuron Function by Regulating M2 Microglia Activation After Mild Traumatic Brain Injury

SSRN

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SSRN 2022年

作者： Tsai, Yih-Jeng Ho, Man-Hau Yen, Chia-Hung Wang, Jia-Yi Burnouf, Thierry Chen, Chia-Yen Wang, Yun Chiang, Yung-Hsiao Hoffer, Barry J. Chou, Szu-Yi Department of Otolaryngology Head and Neck Surgery Shin Kong Wu HoSu Memorial Hospital Taipei Taiwan School of Medicine Fu Jen Catholic University New Taipei City Taiwan Ph.D. Program in Medical Neuroscience College of Medical Science and Technology Taipei Medical University National Health Research Institute Taiwan Graduate Institute of Neural Regenerative Medicine College of Medical Science and Technology Taipei Medical University Taipei Taiwan Department of Biological Science and Technology National Pingtung University of Science and Technology Neipu Pingtung91201 Taiwan Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei11031 Taiwan Department of Neurosurgery Taipei Medical University Hospital Taipei11031 Taiwan Neuroscience Research Center Taipei Medical University Taipei11031 Taiwan International PhD Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei Taiwan NeuroTMULille International Laboratory Taipei Medical University Taipei Taiwan International PhD Program in Cell Therapy and Regeneration Medicine College of Medicine Taipei Medical University Taipei Taiwan Brain and Consciousness Research Centre TMU Shuang Ho Hospital New Taipei City Taiwan Center for Neuropsychiatric Research National Health Research Institutes Zhunan Miaoli County Taiwan Department of Surgery School of Medicine College of Medicine Taipei Medical University 11031 Taiwan Department of Neurosurgery Case Western Reserve University School of Medicine ClevelandOH United States Scientist Emeritus National Institutes of Health United States International Master Program in Medical Neuroscience College of Medical Science and Technology Taipei Medical University Taipei Taiwan

Cytokines and chemokines play important roles in inflammation and repair following brain injury. M1 microglia are pro-inflammatory which produce cytokines and lead to neural injury;in contrast, M2 microglia are anti-inflammatory, release neurotrophic factors, and promote neural repair processes. Chemokine CCL5, increased after TBI, shows a neuroprotective function by reducing oxidative stress. CCL5 may contribute to the balance between oxidative stress and immune responses after brain injury. Herein, we investigated the role of CCL5 in microglial polarization after mild traumatic brain injury, focusing on the cerebral cortex.C57BL/6 and CCL5 knockout (CCL5-KO) mice were given a mild traumatic brain injury (TBI) using weight-drop. Neurological parameters such as motor and sensory functions were analyzed by mNSS score, accelerating rotarod, beam walking, and adhesive removal tests. Oxidative stress and neuron damage were measured by NADPH oxidase activity, hypoxyloprobe staining and FJC. Performance of motor and sensory functions in both WT and CCL5-KO mice were impaired after brain injury which recovered after 7 days-post-injury (dpi) in the WT group but only after 14 days in the CCL5-KO mouse group. RT-qPCR analysis revealed that pro-inflammation cytokines - IL-1β, TNF-α, and IL-6 were higher in CCL5-KO mice compared to WT mice at 4 and 14 dpi. In contrast, M2-like microglia markers - IL-10 and Agr-1 were increased in WT mouse cortical tissue at 4 dpi. Oxidative stress increased both M1 and M2- related cytokine expression in BV2 cells treated with H 2 O 2;CCL5 treatment increased M2- but suppressed M1- related cytokine gene activation. Intranasal delivery of CCL5 reduced neuronal oxidative stress, increased IL-10 expression and improved motor and sensory functions in CCL5-KO mice after brain *** summary, CCL5, which alters immune responses and protects neurons from TBI damage, has an important function in regulating M2-like microglial polarization during post-

关键词： Polarization

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Microfluidic nanodevices for drug sensing and screening applications

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Biosensors and Bioelectronics 2023年 219卷 114783页

作者： Pal, Arnab Kaswan, Kuldeep Barman, Snigdha Roy Lin, Yu-Zih Chung, Jun-Hsuan Sharma, Manish Kumar Liu, Kuei-Lin Chen, Bo-Huan Wu, Chih-Cheng Lee, Sangmin Choi, Dongwhi Lin, Zong-Hong Institute of Biomedical Engineering National Tsing Hua University Hsinchu30013 Taiwan International Intercollegiate PhD Program National Tsing Hua University Hsinchu30013 Taiwan Department of Power Mechanical Engineering National Tsing Hua University Hsinchu30013 Taiwan Department of Materials Science and Engineering National Tsing Hua University Hsinchu30013 Taiwan Department of Chemistry National Tsing Hua University Hsinchu30013 Taiwan Frontier Research Center on Fundamental and Applied Sciences of Matters National Tsing Hua University Hsinchu30013 Taiwan Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital Linkou Medical Center Taoyuan333 Taiwan Center of Quality Management National Taiwan University Hospital Hsinchu Branch Hsinchu30059 Taiwan College of Medicine National Taiwan University Taipei10051 Taiwan Institute of Cellular and System Medicine National Health Research Institute Zhunan 35053 Taiwan School of Mechanical Engineering Chung-Ang University Seoul06974 Korea Republic of Kyung Hee University Gyeonggi 17104 Korea Republic of

The outbreak of pandemics (e.g., severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 in 2019), influenza A viruses (H1N1 in 2009), etc.), and worldwide spike in the aging population have created unprecedented urgency for developing new drugs to improve disease treatment. As a result, extensive efforts have been made to design novel techniques for efficient drug monitoring and screening, which form the backbone of drug development. Compared to traditional techniques, microfluidics-based platforms have emerged as promising alternatives for high-throughput drug screening due to their inherent miniaturization characteristics, low sample consumption, integration, and compatibility with diverse analytical strategies. Moreover, the microfluidic-based models utilizing human cells to produce in-vitro biomimetics of the human body pave new ways to predict more accurate drug effects in humans. This review provides a comprehensive summary of different microfluidics-based drug sensing and screening strategies and briefly discusses their advantages. Most importantly, an in-depth outlook of the commonly used detection techniques integrated with microfluidic chips for highly sensitive drug screening is provided. Then, the influence of critical parameters such as sensing materials and microfluidic platform geometries on screening performance is summarized. This review also outlines the recent applications of microfluidic approaches for screening therapeutic and illicit drugs. Moreover, the current challenges and the future perspective of this research field is elaborately highlighted, which we believe will contribute immensely towards significant achievements in all aspects of drug development. © 2022 Elsevier B.V.

关键词： Microfluidics

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