The quantification of immune cell subpopulations in blood is important for the diagnosis,prognosis and management of various diseases and medical *** cytometry is currently the gold standard technique for cell quantif...
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The quantification of immune cell subpopulations in blood is important for the diagnosis,prognosis and management of various diseases and medical *** cytometry is currently the gold standard technique for cell quantification;however,it is laborious,time-consuming and relies on bulky/expensive instrumentation,limiting its use to laboratories in high-resource *** cytometers offering enhanced portability have been developed that are capable of rapid cell quantification;however,these platforms involve tedious sample preparation and processing protocols and/or require the use of specialized/expensive instrumentation for flow control and cell ***,we report an artificial intelligence-enabled microfluidic cytometer for rapid CD4^(+)T cell quantification in whole blood requiring minimal sample preparation and ***4^(+)T cells in blood are labeled with anti-CD4 antibody-coated microbeads,which are driven through a microfluidic chip via gravity-driven slug flow,enabling pump-free operation.A video of the sample flowing in the chip is recorded using a microscope camera,which is analyzed using a convolutional neural network-based model that is trained to detect bead-labeled cells in the blood *** functionality of this platform was evaluated by analyzing fingerprick blood samples obtained from healthy donors,which revealed its ability to quantify CD4^(+)T cells with similar accuracy as flow cytometry(<10%deviation between both methods)while being at least 4×faster,less expensive,and simpler to *** envision that this platform can be readily modified to quantify other cell subpopulations in blood by using beads coated with different antibodies,making it a promising tool for performing cell count measurements outside of laboratories and in low-resource settings.
Dislocations in ceramics are increasingly recognized for their promising potential in applications such as toughening intrinsically brittle ceramics and tailoring functional ***,the atomistic simulation of dislocation...
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Dislocations in ceramics are increasingly recognized for their promising potential in applications such as toughening intrinsically brittle ceramics and tailoring functional ***,the atomistic simulation of dislocation plasticity in ceramics remains challenging due to the complex interatomic interactions characteristic of ceramics,which include a mix of ionic and covalent bonds,and highly distorted and extensive dislocation core structures within complex crystal *** complexities exceed the capabilities of empirical interatomic ***,constructing neural network potentials(NNPs)emerges as the optimal ***,creating a training dataset that includes dislocation structures proves difficult due to the complexity of their core configurations in ceramics and the computational demands of density functional theory for large atomic models containing dislocation *** this work,we propose a training dataset from properties that are easier to compute via high-throughput calculation.
The inculcation of bioinspiration in sensing and human–machine interface(HMI)technologies can lead to distinctive characteristics such as conformability,low power consumption,high sensitivity,and unique properties li...
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The inculcation of bioinspiration in sensing and human–machine interface(HMI)technologies can lead to distinctive characteristics such as conformability,low power consumption,high sensitivity,and unique properties like self-healing,self-cleaning,and *** sensing and HMI are fields rife with opportunities for the application of bioinspired nanomaterials,particularly when it comes to wearable sensory systems where biocompatibility is an additional *** review discusses recent development in bioinspired nanomaterials for wearable sensing and HMIs,with a specific focus on state-of-the-art bioinspired capacitive sensors,piezoresistive sensors,piezoelectric sensors,triboelectric sensors,magnetoelastic sensors,and electrochemical *** also present a comprehensive overview of the challenges that have hindered the scientific advancement in academia and commercialization in the industry.
One distinct advantage of microfluidic-based cell assays is their scalability for multiple concentrations or *** scaling can be extremely powerful when combining multiple parameters and ***,in situ stimulation and det...
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One distinct advantage of microfluidic-based cell assays is their scalability for multiple concentrations or *** scaling can be extremely powerful when combining multiple parameters and ***,in situ stimulation and detection eliminates variability between individual ***,conventional microfluidics must combat diffusion,which limits the spatial distance and time for molecules traveling through ***,we leveraged a multilayered microfluidic approach to integrate a novel oxygen gradient(0–20%)with an enhanced hydrogel sensor to study pancreatic beta *** enabled our microfluidics to achieve spatiotemporal detection that is difficult to achieve with traditional *** this device,we demonstrated the in situ detection of calcium,insulin,and ATP(adenosine triphosphate)in response to glucose and oxygen ***,insulin was quantified at levels as low as 25 pg/mL using our imaging ***,by analyzing the spatial detection data dynamically over time,we uncovered a new relationship between oxygen and beta cell *** observed an optimum oxygen level between 10 and 12%,which is neither hypoxic nor normoxic in the conventional cell culture *** results provide evidence to support the current islet oscillator *** future applications,this spatial microfluidic technique can be adapted for discrete protein detection in a robust platform to study numerous oxygen-dependent tissue dysfunctions.
A novel analytical system was developed to monitor the environmental hydrogen embrittlement of aluminum alloys dynamically and quantitatively under atmospheric air pressure. Use of this system revealed that hydrogen a...
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The continuous pursuit for a better quality of life promotes continuous advancements in intelligent *** wearable and implantable bioelectronics have emerged as an innovative complement to rigid material-based electron...
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The continuous pursuit for a better quality of life promotes continuous advancements in intelligent *** wearable and implantable bioelectronics have emerged as an innovative complement to rigid material-based electronic devices[1-3].Due to their distinct advantages in terms of ductile,ultrathin,and biocompatible features,these elastic and soft bioelectronic devices can be seamlessly mounted onto various real or artificial tissues and organs.
Over the past 2 decades,cancer stem cells(CSCs)have been identified as the root cause of cancer occurrence,progression,chemoradioresistance,recurrence,and *** CSCs is a novel therapeutic strategy for cancer management...
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Over the past 2 decades,cancer stem cells(CSCs)have been identified as the root cause of cancer occurrence,progression,chemoradioresistance,recurrence,and *** CSCs is a novel therapeutic strategy for cancer management and *** cancer(LC)is a malignant disease that can endanger human *** are increasingly suggesting that changes in the liver mechanical microenvironment are a primary driver triggering the occurrence and development of liver *** this review,we summarize current understanding of the roles of the liver mechano-microenvironment and liver cancer stem cells(LCSCs)in liver cancer *** also discuss the relationship between the mechanical heterogeneity of liver cancer tissues and LCSC recruitment and ***,we highlight potential mechanosensitive molecules in LCSCs and mechanotherapy in liver *** the roles and regulatory mechanisms of the mechano-microenvironment and LCSCs may provide fundamental insights into liver cancer progression and aid in further development of novel therapeutic strategies.
Recursive Dynamics Simulator (ReDySim) is a tool for the dynamic analysis of multibody systems. It has high computational speed, and most importantly, it is freely available. ReDySim is built on the computationally ef...
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Niobium(Nb)is sensitive to even minute quantities of silicon(Si)solutes,which are known to induce pronounced ***,the underlying mechanism for hardening remains elusive since the ef-fect of Si solutes on dislocation be...
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Niobium(Nb)is sensitive to even minute quantities of silicon(Si)solutes,which are known to induce pronounced ***,the underlying mechanism for hardening remains elusive since the ef-fect of Si solutes on dislocation behavior is ***,using tensile testing,in-situ microscopy and nanomechanical testing,the behavior of dislocations in dilute Nb-Si alloys,containing from 0 at.%to 0.8 at.%Si,is *** show that the hardness,strength and strain hardening rate increase from two to four times,while the uniform elongation in tension only reduces 50%as the Si content *** evolve from complex entangled patterns in Nb to parallel long-straight screw dislocation-dominated structures in Nb-Si ***-situ indentation reveals that the origins of the marked harden-ing in Nb-Si alloy are the reduction of dislocation mobility and cross-slip *** densities of dislocation debris-superjogs and loops introduced throughout the sample during warm rolling and an-nealing are found to provide active internal dislocation sources,which explain the minimal ductility loss seen in these Nb-Si *** findings can help guide the alloy design of high-performance refractory materials for extreme temperature applications.
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