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Mild liver injury following withdrawal of long-term prednisonetherapy: A case report

World Journal of Gastroenterology 2025年第4期31卷 144-150页

作者： Jing-Wen Zhu Jun Yan Zhi-Han Zhang Tian-Qi Wang Department of Biochemistry and Molecular Biology School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001Henan ProvinceChina

BACKGROUND Liver injury manifesting as hepatic enzyme abnormalities,has been occasionally identified to be a feature of primary or secondary Addison's disease,an uncommon endocrine disease characterized by adrenal *** have been no more than 30 reported cases of liver injury explicitly attributed to Addison's *** injury resulting from adrenal insufficiency due to glucocorticoid withdrawal is exceptionally *** SUMMARY A 42-year-old man presented with fatigue and mildly elevated *** investigations and imaging studies excluded common etiologies of liver *** on the fact that the patient discontinued long-term therapy with prednisone approximately 2 weeks before he was found to have elevated transaminase levels and the observation that his cortisol was lower than the normal value,he was diagnosed as having hypertransaminasemia secondary to adrenal insufficiency caused by glucocorticoid *** patient was infused intravenously with compound diisopropylamine dichloroacctate and compound glycyrrhizin,and his transaminase levels returned to normal after 1 *** 2 years later,the patient received hydroprednisone treatment for 2 days in an endoscopic sinus *** days after he discontinued the hydroprednisone treatment,he developed symptoms reminiscent of glucocorticoid withdrawal *** symptoms resolved spontaneously after 1 ***,the patient did not develop hepatic dysfunction this *** The present case,showing some unusual clinical features,highlights the importance of education of clinicians and patients to avoid improper discontinuation of glucocorticoid therapy and complete history taking for prompt recognition.

关键词： Liver injury Transaminase Prednisone Addison's disease Adrenal insufficiency Case report

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miR-2940-1 is involved in the circadian regulation of oviposition in Aedes albopictus

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Insect Science 2025年第1期32卷 69-79页

作者： Xiaolin Xiao Ling Kong Zhensheng Xie Hongkai Liu Lijun Cai Siyu Zhao Jiayong Zhou Shuang Liu Jing Wu Yiming Wu Peilin Wu Anthony A.James Xiao-Guang Chen Department of Pathogen Biology Institute of Tropical MedicineSchool of Public HealthSouthern Medical UniversityGuangzhouChina Department of Microbiology&Molecular Genetics University of CaliforniaIrvineCAUSA Department of Molecular Biology&Biochemistry University of CaliforniaIrvineCAUSA

The vast majority of all global species have circadian rhythm cycles that allow them to adapt to natural *** regular rhythms are regulated by core clock genes and recent studies have also implicated roles for microRNAs in this *** is an important circadian behavior in the reproductive cycle of insect vectors of diseases,and little is known about the rhythm or its regulation in *** albopictus is a diurnal mosquito that transmits arboviruses and is the major cause of outbreaks of dengue fever in *** analyzed the oviposition rhythm patterns of *** under different light/dark conditions and show that the mosquitoes have an oviposition peak between zeitgeber time 9(ZT 9)and ZT ***,the antagomir-mediated knockdown of expression of the microRNA miR-2940-1 affected the oviposition rhythm of *** data support the conclusion that miR-2940-1 is involved in the regulation of oviposition rhythm in *** and provide a foundation for using oviposition rhythms as a new target for vector mosquito control.

关键词： Aedes albopictus miR-2940-l oviposition rhythm regulation

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Hypoxia-Associated GPNMB+ Macrophages Promote Malignant Progression of Colorectal Cancer and Its Related Risk Signature Are Powerful Predictive Tool for the Treatment of Colorectal Cancer Patients

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Environmental Toxicology 2025年第2期40卷 204-221页

作者： Zhang, Junli Hu, Shangshang Jin, Xinxin Zheng, Yiwen Yu, Lianchen Ma, Junrao Gu, Biao Wang, Fen Wu, Wenjuan Department of Blood Transfusion The Third People's Hospital of Bengbu Affiliated to Bengbu Medical University Anhui Bengbu China Department of Biochemistry and Molecular Biology Bengbu Medical University Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis Bengbu Medical University Anhui Bengbu China Department of Clinical Medicine School of Medicine Southeast University Jiangsu Nanjing China Department of Biochemistry and Molecular Biology School of Laboratory Medicine Bengbu Medical College Bengbu China

Colorectal cancer (CRC) is a highly malignant tumor with hypoxia being a crucial feature during its progression. This study utilized multiple independent CRC cohorts for bioinformatics analysis and in vitro experiments to investigate the role of hypoxia-related subgroups in CRC. Machine learning was employed to construct risk features associated with this subgroup and further explore its therapeutic value in CRC. The study identified the GPNMB+ Macrophage (GPNMB+ Macr) subgroup as most relevant to hypoxia. GPNMB+ Macr showed significantly higher infiltration in tumor tissues compared to non-tumor tissues, increasing with CRC stage. High infiltration of GPNMB+ Macr was associated with poor prognosis in terms of overall and recurrence-free survival in CRC patients. GPNMB+ Macrophages exhibit M2-like characteristics and have the ability to promote 5-FU resistance, proliferation, and metastasis of CRC cells. The study developed the Hypoxia-Related Macrophage Risk Score (HMRS), which not only served as an independent prognostic factor for CRC patients but also demonstrated robust prognostic performance compared to 84 previously published prognostic features. Patients with low HMRS were sensitive to fluorouracil, oxaliplatin (FOLFOX), and anti-PD-1 immunotherapy, while those with high HMRS showed resistance. Additionally, HMRS was identified as an independent prognostic factor in other digestive tract tumors (hepatocellular carcinoma, pancreatic cancer, esophageal cancer, and gastric cancer), indicating potential extrapolation to other tumor types. In conclusion, GPNMB+ Macr promotes the malignant progression of CRC, and HMRS serves as a powerful predictive tool for prognosis, chemotherapy, and immunotherapy in CRC patients, aiding in improving the quality of survival. © 2024 Wiley Periodicals LLC.

关键词： Macrophages

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ARPC1A Promotes NSCLC Malignancy via Stimulating the Drug Resistance and Cell Migration

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BIOCELL 2025年第3期49卷 483-502页

作者： Hongjuan Guo Dan Liu Ruyu Yan Tianjing Zhang Kecheng Zhou Minxia Liu School of Life Sciences Anhui Medical UniversityHefei230032China Department of Biochemistry and Molecular Biology School of Basic Medical SciencesAnhui Medical UniversityHefei230032China Department of Orthopaedics Hejin Shengji HospitalYuncheng043300China

Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic *** identification of potential biomarkers delineating the progression and metastatic cascade of NSCLC assumes paramount importance in fostering advancements toward enhanced patient outcomes and prognostic ***:The expression level of the actin-related protein 2/3 complex;subunit 1A(ARPC1A)in NSCLC was evaluated using The Cancer Genome Atlas(TCGA)and Gene Expression Profiling Interactive Analysis(GEPIA)databases;along with the LinkedOmics database for co-expression *** verification of ARPC1A expression in normal lung cells and NSCLC cells;as well as in normal tissues and lung cancer tissues;was performed using quantitative real-time reverse transcription PCR(RTqPCR)and Western *** function of ARPC1A was explored through Gene Set Enrichment Analysis(GSEA)and immune infiltration analysis;followed by functional experiments for ***:ARPC1A is upregulated in NSCLC and is associated with unfavorable clinical ***,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis highlights a potential link between the ARPC1A gene and the cell cycle and p53 signaling ***1A also promotes cell proliferation and resistance to chemotherapeutic drugs,thereby enhancing the oncogenic potential of *** cell-based experiments confirm that targeted inhibition of ARPC1A effectively suppresses cellular migratory and invasive *** immune infiltration analysis showed a close association between ARPC1A expression and various immune components,suggesting ARPC1A may interact with the tumor ***,ARPC1A promotes cell migration by stimulating the epithelialto-mesenchymal transition(EMT).Conclusion:The study results revealed the potential of ARPC1A as a valuable prognostic bioma

关键词： NSCLC ARPC1A drug resistance EMT immune infiltration dynamics

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NUMB endocytic adaptor protein(NUMB)mediates the anti-hepatic fibrosis effect of artesunate(ART)by inducing senescence in hepatic stellate cells(HSCs)

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Chinese Journal of Natural Medicines 2025年第3期23卷 322-333页

作者： Yangling Qiu Yujia Li Mengran Li Yingqian Wang Min Shen Jiangjuan Shao Feng Zhang Xuefen Xu Feixia Wang Zili Zhang Shizhong Zheng Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica Nanjing University of Chinese MedicineNanjing 210023China Department of Biochemistry and Molecular Biology Medical CollegeYangzhou UniversityYangzhou 225009China

Developing and identifying effective medications and targets for treating hepatic fibrosis is an urgent *** previous research demonstrated the efficacy of artesunate(ART)in alleviating liver fibrosis by eliminating activated hepatic stellate cells(HSCs).However,the underlying mechanism remains unclear despite these ***,endocytic adaptor protein(NUMB)has significant implications for treating hepatic diseases,but current research primarily focuses on liver regeneration and hepatocellular *** precise function of NUMB in liver fibrosis,particularly its ability to regulate HSCs,requires further *** study aims to elucidate the role of NUMB in the anti-hepatic fibrosis action of ART in *** observed that the expression level of NUMB significantly decreased in activated HSCs compared to quiescent HSCs,exhibiting a negative correlation with the progression of liver ***,ART induced senescence in activated HSCs through the NUMB/P53 tumor suppressor(P53)*** identified NUMB as a crucial regulator of senescence in activated HSCs and as a mediator of ART in determining cell *** research examines the specific target of ART in eliminating activated HSCs,providing both theoretical and experimental evidence for the treatment of liver fibrosis.

关键词： Hepatic stellate cells NUMB endocytic adaptor protein Senescence Artesunate Ubiquitylation Liver fibrosis

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Development of a Novel Peptide with RGD Tumor Homing Motif: Evaluation of its Anticancer Potential in Hepatocellular Carcinoma and Colon Cancer Cells

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Recent Patents on Biotechnology 2025年第2期19卷 128-141页

作者： Mohamed, Reda Abdallah Marie, Ohoud Mohammed Badran, Dahlia Ibrahim Hammam, Olfat Ali Ahmed, Hend Okasha Department of Chemistry Faculty of Science Suez Canal University Ismailia Egypt Department of Medical Biochemistry Faculty of Medicine Suez Canal University Ismailia Egypt Department of Pathology Theodor Bilharz Research Institute Giza Egypt Department of Biochemistry and Molecular Biology Theodor Bilharz Research Institute Giza Egypt

Background: Peptide-based therapy has emerged as a promising avenue for treating various disorders, and recent research has highlighted the potential of anti-cancer peptides (ACPs) in cancer treatment. In this context, this study aimed to design a novel peptide incorporating a tumor-homing peptide (RGD) and C-amidation to enhance its anticancer activity, particularly against liver (HepG2) and colon (HCT-116) cancer cell lines. Objectives: The primary objective was to design a peptide with improved anticancer properties by leveraging the tumor-homing capabilities of RGD and enhancing its activity through C-amidation. The study sought to evaluate the cytotoxicity of the designed peptide against red blood cells (RBCs) and normal Vero cells. Furthermore, the anticancer efficacy of the peptide was assessed in hepatocellular carcinoma (HepG2) and colon cancer (HCT-116) cell lines. The specific objectives included examining the apoptot-ic induction and morphological changes in treated cells compared to untreated cells. Methods: The peptide was designed using the ACPred-FL bioinformatics tool, and its cytotoxicity was assessed through hemolysis assays against RBCs and normal Vero cells. Anticancer activity was evaluated against HepG2 and HCT-116 cell lines. The analysis of apoptotic induction involved measuring the relative gene expression of oncogenic marker BCL2 and apoptotic markers (BAX, BID, CAS-8). Additionally, Cytopathologi-cal examination and Western Blot analysis were employed to study morphological changes and confirm the quantification of relevant markers. Results: The designed peptide, consisting of twelve amino acids with a molecular mass of 1230.6233 Da and an isoelectric point of 9.81, exhibited low erythrocyte lysis and minimal toxicity to normal cells. The IC50 values demonstrated significant anticancer activity against both HepG2 (36.49±2.6 µg/mL) and HCT-116 (11.03±2.5 µg/mL) cell lines. Treated cells exhibited a significant decrease in the oncogenic mar

关键词： Gene expression

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A pioneering review on Ganoderma lucidum–derived leather: taking a step towards a cruelty-free leather manufacturing

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Environmental Science and Pollution Research 2025年第10期32卷 5730-5746页

作者： Sahu, Sudarshan Bhardwaj, Priyanka Singh, Gursharan Bhalla, Aditya Arya, Shailendra Kumar Department of Biotechnology University Institute of Engineering and Technology Panjab University Chandigarh India Department of Medical Laboratory Sciences Lovely Professional University Punjab Phagwara144411 India Department of Biochemistry and Molecular Biology Michigan State University East Lansing United States

Ganoderma lucidum–derived leather presents a sustainable alternative to traditional leather, addressing ethical, environmental, and socio-economic concerns in the fashion industry. This study explores the advantages of G. lucidum–derived leather, including its cruelty-free nature, eco-friendly characteristics, and positive social and economic implications. By eliminating the need for animal exploitation, G. lucidum–derived leather aligns with ethical consumer preferences and promotes compassion towards animals. Additionally, its production process is environmentally friendly, utilizing organic substrates and avoiding toxic chemicals, thereby reducing pollution and minimizing ecological footprints. Furthermore, G. lucidum–derived leather offers significant social and economic benefits, enhancing brand reputation and attracting socially conscious consumers. Its cultivation creates economic opportunities in rural communities, stimulating local economies and providing jobs in mushroom cultivation and leather manufacturing. However, challenges remain, such as the need for further research on its mechanical properties and biodegradability. G. lucidum–derived leather represents a promising solution to the challenges facing the fashion industry, offering a sustainable and ethical alternative that meets the demands of conscious consumers and promotes environmental stewardship. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.

关键词： Leather

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Identification of AAV serotypes for gene therapy in Krabbe iPSCs-derived brain organoids

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Genes & Diseases 2025年第1期12卷 144-147页

作者： Yafeng Lv Zhi Cui Hongbo Li Jing Wang Mulan Wei Yuanlang Hu Xun Li Chunyu Cao Ye Zhang Wei Wang Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy College of Basic Medical SciencesChina Three Gorges UniversityYichangHubei 443000China State Key Laboratory of Medical Molecular Biology Department of Biochemistry and Molecular BiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China Yiling People’s Hospital of Yichang YichangHubei 443000China China-Japan Friendship Hospital Beijing 100029China

Krabbe disease,also known as globoid cell leukodystrophy,is a rare lysosomal storage *** is primarily caused by mutations in the GALC gene on chromosome 14q31,leading to GALC enzyme deficiency in *** results in the accumulation of toxic substrate psychosine in the nervous systems.1 Currently,hematopoietic stem cell transplantation is the only available treatment,offering only a delay in neurological *** therapy,particularly using recombinant adeno-associated viruses(AAVs),shows promise for treating genetic diseases by introducing functional genes into target cells.

关键词： AAV dystrophy diseases

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Interplay and cooperation between GLI2 and master transcription factors promote progression of esophageal squamous cell carcinoma

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American Journal of Human Genetics 2025年第5期112卷 1039-1061页

作者： Liu, Yin-Qiao Zheng, Ze-Jun Fang, Wang-Kai Li, Yan-Shang Li, Chun Yang, Min Han, Dong-Chen Zhou, Jun-Hua Xie, Ying-Hua Zhang, Yu-Ying Kang, Zhuo-Ying Xu, Yi-Wei Xie, Jian-Jun Department of Clinical Laboratory Medicine Chaoshan Branch of State Key Laboratory for Esophageal Cancer Prevention and Treatment the Cancer Hospital of Shantou University Medical College Shantou 515041 China Department of Biochemistry and Molecular Biology Shantou University Medical College Shantou 515041 China Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics Institute of Biochemistry & Molecular Biology Institute of Aging Research Guangdong Medical University Dongguan 523808 China Beijing University of Chinese Medicine Fangshan 102400 China

The establishment of gene expression programs that drive cell identity is governed by tightly regulated transcription factors (TFs) that engage in auto- and cross-regulation in a feedforward manner, forming core regulatory circuitries (CRCs). Here, we identify and validate an important interconnected CRC formed by three master TFs—GLI2, TP63, and RUNX1—in esophageal squamous cell carcinoma (ESCC). Furthermore, master TFs co-bind to their own and each other's super-enhancers, forming an interconnected auto-regulatory loop. Mechanistically, these master TFs occupy the majority of ESCC super-enhancers and cooperatively orchestrate the ESCC transcription program. Functionally, GLI2, a master TF, is essential for ESCC viability, migration, invasion, and the growth of xenograft tumors. Moreover, the overexpression of GLI2 is significantly associated with shorter overall survival of patients with ESCC. Downstream, this CRC apparatus coordinately regulates gene expression networks in ESCC, controlling important cancer-promoting pathways, including Hedgehog, glycolysis, and epidermal growth factor receptor signaling pathways. Together, these findings offer significant mechanistic insights into the transcriptional dysregulation in ESCC and recognize GLI2 as a potential therapeutic target and prognostic marker for ESCC. More importantly, CRC-downstream genes and signaling pathways may contain potential therapeutic targets for this malignancy. © 2025 American Society of Human Genetics

关键词： esophageal cancer gene regulation oncogenesis signal transduction transcription factor

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Non-invasive tools for analysis of plasma membrane protein topology in living cells

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Methods 2025年 239卷 111-126页

作者： Savenkova, Daria Bulatova, Leysan Skripova, Vera Kiyamova, Ramziya Bogdanov, Mikhail Department of Biochemistry Biotechnology and Pharmacology Kazan (Volga Region) Federal University Tatarstan Kazan Russian Federation Department of Biochemistry and Molecular Biology McGovern Medical School at The University of Texas Health Science Center at Houston 77030 TX United States

Membrane protein topology studies offer guidance to membrane protein structure, folding, and function, serving as a credible scaffold for designing site-directed mutagenesis and biochemical experiments, helping to identify functionally significant extracellular and intracellular regions, modeling three-dimensional structures, and building reliable mechanistic models. Membrane protein structure as a function of given lipid composition and physiological state of the cell is best probed in whole intact cells. A described simple and advanced immunofluorescence protocol applied to the transmembrane orientation of extramembrane domains permits a topology analysis of plasma membrane proteins in their native state in living unperturbed eucaryotic cells. The accessibility of native epitopes to corresponding antibodies is determined in intact and permeabilized cells to establish their extra- or intracellular or localization respectively. The ability of the given antibody to bind the epitope in intact live and permeabilized cells is then assessed routinely by intact and permeabilized cell immunofluorescent confocal microscopy or fluorescence flow cytometry parametric analyses in several hours. To ensure that the observed immunofluorescence is entirely a result of the binding of antibodies, cells are alive and the plasma membrane is intact, plasma membrane integrity is routinely monitored by co-incubating the cells with a cell membrane-impermeable probe, propidium iodide. Accordingly, plasma membrane side-specific immunostaining analysis was restricted to the propidium iodide-negative, non-permeabilized cell population. The strength of this technique is its simplicity since each native epitope is unique and there is no need to mutate any endogenous sites, introduce new epitopes, or engineer single, dual, or split colorimetric enzymatic reporters. Aside from its simplicity, the advantage of this approach is that the topology is documented in the context of full-length and fully

关键词： Antibodies Confocal microscopy Flow cytometry Fluorescence Membrane protein Plasma membrane Propidium iodide Topology

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