Metabolism is vital to every aspect of cell function, yet the metabolome of induced pluripotent stem cells (iPSCs) remains largely unexplored. Here we report, using an untargeted metabolomics approach, that human iPSC...
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Metabolism is vital to every aspect of cell function, yet the metabolome of induced pluripotent stem cells (iPSCs) remains largely unexplored. Here we report, using an untargeted metabolomics approach, that human iPSCs share a pluripotent metabolomic signature with embryonic stem cells (ESCs) that is distinct from their parental cells, and that is characterized by changes in metabolites involved in cellular respiration. Examination of cellular bioenergetics corroborated with our metabolomic analysis, and demonstrated that somatic cells convert from an oxidative state to a glycolytic state in pluripotency. Interestingly, the bioenergetics of various somatic cells correlated with their reprogramming efficiencies. We further identified metabolites that differ between iPSCs and ESCs, which revealed novel metabolic pathways that play a critical role in regulating somatic cell reprogramming. Our findings are the first to globally analyze the metabolome of iPSCs, and provide mechanistic insight into a new layer of regulation involved in inducing pluripotency, and in evaluating iPSC and ESC equivalence.
作者：
Jong Hwan BaekNam Su HeoMee-Jung HanSang Yup LeeMetabolic and Biomolecular Engineering National Research Laboratory
Department of Chemical (&) Biomolecular Engineering (BK21 Program) BioPracess Engineering Research Center Center for Systems and Synthetic Biotechnology and Institute for the BioCentury Republic of Korea Metabolic and Biomolecular Engineering National Research Laboratory Department of Chemical (&) Biomolecular Engineering (BK21 Program) BioPracess Engineering Research Center Center for Systems and Synthetic Biotechnology and Institute for the BioCentury Republic of Korea Department of Bio and Brain Engineering
and Bioinformatics Research Center KAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of Korea
作者：
Xiao-Xia XiaSang Yup LeeDepartment of Bio and Brain Engineering
and Bioinformatics Research Center KAIST Daejeon Republic of Korea Metabolic and Biomolecular Engineering National Research Laboratory
Department af Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center Center for Sys tems and Synthetic Biotechnology and Institute for the BioCenturyKAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of Korea Metabolic and Biomolecular Engineering National Research Laboratory Department af Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center Center for Sys tems and Synthetic Biotechnology and Institute for the BioCenturyKAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of Korea
作者：
Zhi-Gang QianXiao-Xia XiaJong Hyun ChoiSang Yup LeeDepartment of Bio and Brain Engineering
and Bioinformatics Research Center KAIST Daejeon Republic of Korea Metabolic and Biomolecular Engineering National Research Labora tory
Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center Center for Systems and Synthetic Biotechnology and Institute for the BioCenturyKAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of Korea Metabolic and Biomolecular Engineering National Research Labora tory Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center Center for Systems and Synthetic Biotechnology and Institute for the BioCenturyKAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of Korea
作者：
Xiao-Xia XiaZhi-Gang QianMee-Jung HanSang Yup LeeMetabolic and Biomolecular Engineering National Research Lahoratory
Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center Cen terror Sys tems and Synthetic Biotechnology and Institute for the BioCenturyKAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of Korea Metabolic and Biomolecular Engineering National Research Lahoratory Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center Cen terror Sys tems and Synthetic Biotechnology and Institute for the BioCenturyKAIST 335 Gwahangno Yuseong-gu Daejeon 305-701 Republic of KoreaDepartment of Bio and Brain Engineering and Bioinformatics Research Center KAIST Daejeon Republic of Korea
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