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Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography

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PhotoniX 2024年第1期5卷 605-621页

作者： Xiangyu Guo Jingjing Zhao Liqun Sun Varun Gupta Lin Du Komal Sharma Aidan Van Vleck Kaitlyn Liang Liangcai Cao Lingjie Kong Yuanmu Yang Yong Huang Adam de la Zerda Guofan Jin State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentTsinghua UniversityBeijing 100084China Department of Structural Biology Stanford University School of MedicineStanford UniversityStanfordCA 94305USA Department of Neurosurgery Stanford University School of MedicineStanford UniversityStanfordCA 94305USA Department of Radiology Stanford University School of MedicineStanford UniversityStanfordCA 94305USA Department of Electrical Engineering and Computer Sciences University of CaliforniaBerkeley 94720USA School of Optics and Photonics Beijing Institute of TechnologyNo.5 South Zhongguancun StreetHaidianBeijing 100081China Biophysics Program Molecular Imaging Programand Bio-X Program at Stanford UniversityStanfordCA 94305USA Chan Zuckerberg Biohub San FranciscoCA 94110USA Institute of Medical Equipment Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China

Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical ***,high-resolution imaging in optical microscopy fundamentally requires a tight optical focus,and thus a limited depth of field(DOF).Consequently,large-scale,non-stitched,high-resolution images of curved surfaces,like brains,are difficult to acquire without z-axis *** overcome this limitation,we developed a needle-shaped beam optical coherence tomography angiography(NB-OCTA)system,and for the first time,achieved a volumetric resolution of less than 8μm in a non-stitched volume space of 6.4 mm×4 mm×620μm in *** system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam(GB-OCTA).We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo,and quantitatively analyzed the vessel area density(VAD)and the diameters of representative vessels in different regions over 10 days,revealing different spatiotemporal dynamics in the acute,sub-acute and chronic phase of post-ischemic *** from our NB-OCTA,we revealed that the recovery process is not only the result of spontaneous reperfusion,but also the formation of new *** study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.

关键词： Optical coherence tomography angiography Diffractive optical elements Needle-shaped beam Brain imaging Stroke Cortical blood perfusion

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Wavelength dependence of laser-induced nanowelded microstructures assembled from metal nanoparticles

arXiv

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arXiv 2023年

作者： Rogers, Ariel Niyonshuti, Isabelle I. Ou, Jun Shrestha, Diksha Chen, Jingyi Wang, Yong Department of Physics University of Arkansas FayettevilleAR72701 United States Department of Chemistry and Biochemistry University of Arkansas FayettevilleAR72701 United States School of Engineering California State Polytechnic University Humboldt ArcataCA95521 United States Mechanical Engineering Program California State Polytechnic University Humboldt ArcataCA95521 United States Cell and Molecular Graduate Program University of Arkansas FayettevilleAR72701 United States Materials Science and Engineering Program University of Arkansas FayettevilleAR72701 United States

Light-based nanowelding of metallic nanoparticles is of particular interest because it provides convenient and controlled means for the conversion of nanoparticles into microstructures and fabrication of nanodevices. Here, we demonstrated the wavelength dependence of laser-induced nanowelded shapes of silver nanoparticles (AgNPs). We observed that the nanowelded microstructures illuminated by the 405 nm laser only were more branched than those formed with illumination of both the 405 nm and 532 nm lasers. We quantified this observation by several compactness descriptors and examined the dependence of the power of the 532 nm laser. More importantly, to understand the experimental observations, we formulated and tested a hypothesis by calculating the wavelength-dependent electric field enhancement due to surface plasmon resonance of the AgNPs and nanowelded microstructures when illuminated with lights at the two wavelengths. Based on the different patterns of "hot spots" for welding AgNPs from these calculations, numerical simulations successfully "reproduced" the different shapes of nanowelded microstructures, supporting our hypothesis. This work suggests the possibility of light-based controlling the shapes of laser-induced nanowelded microstructures of metallic nanoparticles. This work is expected to facilitate the development of better nanowelding strategies of metallic nanoparticles for broader applications. Copyright © 2023, The Authors. All rights reserved.

关键词： Surface plasmon resonance

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Low Threshold Plasmonic Lattice Laser Based on CsPbBr3 Quantum Dots with Directional Emission

Low Threshold Plasmonic Lattice Laser Based on CsPbBr3 Quant...

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Pacific Rim Conference on Lasers and Electro-Optics (CLEO/Pacific Rim)

作者： Di Xing Cheng-Chieh Lin Ya-Lun Ho Yang-Chun Lee Mu-Hsin Chen Bo-Wei Lin Chun-Wei Chen Jean-Jacques Delaunay School of Engineering The University of Tokyo Tokyo Japan International Graduate Program of Molecular Science and Technology (NTU-MST) National Taiwan University and Academia Sinica Taipei Taiwan Department of Materials Science and Engineering National Taiwan University Taipei Taiwan Molecular Science and Technology Program Taiwan International Graduate Program (TIGP) Academia Sinica Taipei Taiwan Center of Atomic Initiative for New Materials (AI-MAT) National Taiwan University Taipei Taiwan

Metallic nanoparticles array is served as a promising way to realize on-chip lasers. To achieve high-performance lasers, efficient gain media are also critical. Here, we present a pressure-assisted process for CsPbBr3...

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Performance of 500 liter stainless steel portable biogas anaerobic digester with agitator designed for the tropical developing country 11th

Performance of 500 liter stainless steel portable biogas ana...

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11th International Conference on Materials and Manufacturing Technology, ICMMT 2020

作者： Sebayuana, Kadek Nindhia, Tjokorda Gde Tirta Surata, I. Wayan Nindhia, Tjokorda Sari Shukla, Shailendra Kumar Khanal, Samir Kumar Master student at Ma ster Study Program of Mechanical Engineering Engineering Faculty Udayana University Jimbaran Bali80361 Indonesia Study Program of Mechanical Engineering Engineering faculty Udayana University Jimbaran Bali80361 Indonesia Faculty of Veterinary medicine Udayana University Denpasar Bali802232 Indonesia Departmen of Mechanical Engineering IIT Banaras Hindu University Varanasi221005 India Dept. of Molecular Biosciences and Bioengineering University of Hawaii at Manoa HonoluluHI96822 United States

ISBN: (纸本)9783035737226

This document it is established that 2 types of biogas anaerobic digester that usually found in developing country. First type is fixed dome type of anaerobic digester and secondly is floating drum biogas anaerobic digester. Both of this type have draw back that the anaerobic process is not completed with agitation process that yield low rate of biogas production. Other serious problem is the release of slurry cannot optimal. Some of the slurry will still remain stay in the anaerobic digester especially scum. To drain and cleaning the fixed dome or floating drum biogas anaerobic digester is mandatory to be done regularly that consume time and cost. The fixed dome types as well as floating drum type are usually not portable which cause no possibility to relocate in the new site. It is the purpose of this work to introduce portable biogas anaerobic digester that suitable for developing country with volume of digester around 500 liter of slurry. The requirement of agitator is facilitated, and the anaerobic digester is possible to be operated in batch system or continuous system. The material that is used for anaerobic digester is stainless steel 304 with tungsten inert gas welding technology that is used for the manufacturing. With this design the anaerobic digester are easy to be maintenance. The batch system can keep producing biogas until 52 day with total production of biogas around 3320 liters. The continuous system was conducted by releasing for about 5 liters slurry from the outlet and after that filled with new slurry with stirring with agitator was conducted for about 10 minutes for 3 times in a day (morning, noon, and evening). The result for continuous system is a linear biogas production with rate biogas production is around 51.7 liter/day (1550 liter/30days). © 2021 Trans Tech Publications Ltd, Switzerland.

关键词： Biogas

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Growth of Wafer-Scale Single-Crystal 2D Semiconducting Transition Metal Dichalcogenide Monolayers

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Advanced Science 2024年第11期11卷 e2307839页

作者： Singh, Jitendra Astarini, Nadiya Ayu Tsai, Meng-Lin Venkatesan, Manikandan Kuo, Chi-Ching Yang, Chan-Shan Yen, Hung-Wei Department of Materials Science and Engineering National Taiwan University of Science and Technology Taipei City106335 Taiwan Department of Physics Udit Narayan Post Graduate College Padrauna Uttar Pradesh Kushinagar274304 India Department of Molecular Science and Engineering Institute of Organic and Polymeric Materials National Taipei University of Technology Taipei City106344 Taiwan Institute and Undergraduate Program of Electro-Optical Engineering National Taiwan Normal University Taipei City11677 Taiwan Department of Materials Science and Engineering National Taiwan University Taipei City106319 Taiwan

Due to extraordinary electronic and optoelectronic properties, large-scale single-crystal two-dimensional (2D) semiconducting transition metal dichalcogenide (TMD) monolayers have gained significant interest in the development of profit-making cutting-edge nano and atomic-scale devices. To explore the remarkable properties of single-crystal 2D monolayers, many strategies are proposed to achieve ultra-thin functional devices. Despite substantial attempts, the controllable growth of high-quality single-crystal 2D monolayer still needs to be improved. The quality of the 2D monolayer strongly depends on the underlying substrates primarily responsible for the formation of grain boundaries during the growth process. To restrain the grain boundaries, the epitaxial growth process plays a crucial role and becomes ideal if an appropriate single crystal substrate is selected. Therefore, this perspective focuses on the latest advances in the growth of large-scale single-crystal 2D TMD monolayers in the light of enhancing their industrial applicability. In the end, recent progress and challenges of 2D TMD materials for various potential applications are highlighted. © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.

关键词： Single crystals

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Resolving Contradictory Estimates of Band Gaps of Bulk PdSe2: A Wannier-Localized Optimally-Tuned Screened Range-Separated Hybrid Density Functional Theory Study

arXiv

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arXiv 2025年

作者： Florio, Fred Camarasa-Gómez, María Ohad, Guy Naveh, Doron Kronik, Leeor Ramasubramaniam, Ashwin Department of Mechanical and Industrial Engineering University of Massachusetts Amherst AmherstMA01003 United States Department of Molecular Chemistry and Materials Science Weizmann Institute of Science Rehovoth7610001 Israel Institute for Nanotechnology and Advanced Materials Bar-Ilan University Ramat Gan52900 Israel Materials Science and Engineering Graduate Program University of Massachusetts Amherst AmherstMA01003 United States

Palladium diselenide (PdSe2)—a layered van der Waals material—is attracting significant attention for optoelectronics due to the wide tunability of its band gap from the infrared through the visible range as a function of the number of layers. However, there continues to be disagreement over the precise nature and value of the optical band gap of bulk PdSe2, owing to the rather small value of this gap that complicates experimental measurements and their interpretation. Here, we design and employ a Wannier-localized optimally-tuned screened range-separated hybrid (WOT-SRSH) functional to investigate the electronic bandstructures and optical absorption spectra of bulk and monolayer PdSe2. In particular, we account carefully for the finite exciton center-of-mass momentum within a time-dependent WOT-SRSH framework to calculate the indirect optical gap and absorption onset accurately. Our results agree well with the best available photoconductivity measurements, as well as with state-of-the-art many-body perturbation theory calculations, confirming that bulk PdSe2 has an optical gap in the mid-infrared (upper-bound of 0.44 eV). More generally, this work further bolsters the utility of the WOT-SRSH approach for predictive modeling of layered semiconductors. Copyright © 2025, The Authors. All rights reserved.

关键词： Layered semiconductors

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Bioluminescence-Activated Proximity Labeling for Spatial Multi-Omics

SSRN

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SSRN 2024年

作者： Wang, Ruixiang Fang, Yuxin Hu, Youyue Liu, Yanjun Chen, Peng R. Zou, Peng College of Chemistry and Molecular Engineering Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering Ministry of Education PKU-IDG/McGovern Institute for Brain Research Peking University Beijing100871 China Peking-Tsinghua-NIBS Joint Graduate Program Peking University Beijing100871 China National Key Laboratory of Crop Genetic Improvement Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan430070 China Academy for Advanced Interdisciplinary Studies PKU-Tsinghua Center for Life Science Peking University Beijing100871 China Beijing102206 China

Mapping the spatial organization of proteins and cellular interactions is crucial for understanding their biological functions. Herein, we report a biocompatible, multi-functional luminescence-activated proximity labeling (LAP) strategy for profiling subcellular proteomes and cell-cell interactions in live cells and animals. Our method capitalizes on fusing the photocatalyst miniSOG to NanoLuc luciferase, whose bioluminescence activates miniSOG via a resonance energy transfer mechanism, generating reactive oxygen species in situ to mediate proximity labeling. We demonstrated the high spatial specificity of LAP in a C57BL6/N mouse model transplanted with MC38 cells. Our data revealed tumor microenvironment-dependent remodeling of secretome. LAP was further applied to identify ligand-receptor mediated cell-cell interactions both in vitro and in vivo. We also achieved local transcriptome profiling by combining LAP with next-generation sequencing. Overall, LAP was proved to be a versatile PL technique with strong biocompatibility for spatial multi-omic applications. © 2024, The Authors. All rights reserved.

关键词： Bioluminescence

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Microengineered platforms for characterizing the contractile function of in vitro cardiac models

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Microsystems & Nanoengineering 2022年第1期8卷 29-50页

作者： Wenkun Dou Manpreet Malhi Qili Zhao Li Wang Zongjie Huang Junhui Law Na Liu Craig A.Simmons Jason T.Maynes Yu Sun Department of Mechanical and Industrial Engineering University of TorontoTorontoON M5S 3G8Canada Program in Molecular Medicine The Hospital for Sick ChildrenTorontoON M5G 1X8Canada Department of Biochemistry University of TorontoTorontoON M5S 1A8Canada Institute of Robotics and Automatic Information System and the Tianjin Key Laboratory of Intelligent Robotics Nankai UniversityTianjin 300350China School of Mechanical&Automotive Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China School of Mechatronics Engineering and Automation Shanghai UniversityShanghai 200444China Institute of Biomedical Engineering University of TorontoTorontoON M5S 3G9Canada Translational Biology&Engineering Program Ted Rogers Centre for Heart ResearchTorontoON M5G 1M1Canada Department of Anesthesiology and Pain Medicine University of TorontoTorontoON M5S 1A8Canada Department of Anesthesia and Pain Medicine The Hospital for Sick ChildrenTorontoON M5G 1X8Canada Department of Electrical and Computer Engineering University of TorontoTorontoON M5S 3G4Canada Department of Computer Science University of TorontoTorontoON M5T 3A1Canada

Emerging heart-on-a-chip platforms are promising approaches to establish cardiac cell/tissue models in vitro for research on cardiac physiology,disease modeling and drug cardiotoxicity as well as for therapeutic *** still exist in obtaining the complete capability of in situ sensing to fully evaluate the complex functional properties of cardiac cell/tissue *** to contractile strength(contractility)and beating regularity(rhythm)are particularly important to generate accurate,predictive *** new platforms and technologies to assess the contractile functions of in vitro cardiac models is essential to provide information on cell/tissue physiologies,drug-induced inotropic responses,and the mechanisms of cardiac *** this review,we discuss recent advances in biosensing platforms for the measurement of contractile functions of in vitro cardiac models,including single cardiomyocytes,2D monolayers of cardiomyocytes,and 3D cardiac *** characteristics and performance of current platforms are reviewed in terms of sensing principles,measured parameters,performance,cell sources,cell/tissue model configurations,advantages,and *** addition,we highlight applications of these platforms and relevant discoveries in fundamental investigations,drug testing,and disease ***,challenges and future outlooks of heart-on-a-chip platforms for in vitro measurement of cardiac functional properties are discussed.

关键词： cardiac contractile regularity

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Key Parameters in Determining the Reactivity of Lithium Metal Battery

arXiv

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arXiv 2023年

作者： Lu, Bingyu Cheng, Diyi Sreenarayanan, Bhagath Li, Weikang Bhamwala, Bhargav Bao, Wurigumula Meng, Ying Shirley Department of NanoEngineering University of California San Diego La Jolla CA92093 United States Materials Science and Engineering Program University of California San Diego La Jolla CA92093 United States Pritzker School of Molecular Engineering University of Chicago ChicagoIL60637 United States

Lithium (Li) metal anodes are essential for developing next-generation high-energy-density batteries. Numerous concerns on the potential safety hazards of the Li metal have been brought up before its massive application in commercialized battery packs. However, few investigations have been performed to systematically evaluate the reactivity of Li metal anode in full cell level. Here, differential scanning calorimetry (DSC) with in situ Fourier-transform infrared spectroscopy (FTIR) are used to quantitatively investigate the Li metal reactivity. Lithiated graphite (Li-Gr) and lithiated silicon (Li-Si) are also studied as the comparison samples. The reactivity of the plated Li when coupled with different electrolyte composition, morphology, and atmosphere is systematically studied. More importantly, the reactivity of Li metal full cell with different cathode materials (NMC622, LFP and LNMO) has been compared. It was found that all cell components, including electrolyte composition, Li morphology, the control of inactive Li accumulation and cathode stability, are essential in controlling the reactivity of the plated Li. After optimizing these conditions, the Li metal full cell shows no significant thermal reaction up to 400°C. This work identifies the key parameters in controlling the reactivity of the plated Li and may facilitate lithium metal battery design and manufacturing in the coming future. © 2023, CC BY.

关键词： Differential scanning calorimetry

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Correction: Materials laboratories of the future for alloys, amorphous, and composite materials (MRS Bulletin, (2025), 50, 2, (190-207), 10.1557/s43577-024-00846-y)

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MRS Bulletin 2025年 1-2页

作者： Banerjee, Sarbajit Meng, Y. Shirley Minor, Andrew M. Zhang, Minghao Zaluzec, Nestor J. Chan, Maria K.Y. Seidler, Gerald McComb, David W. Agar, Joshua Mukherjee, Partha P. Melot, Brent Chapman, Karena Guiton, Beth S. Klie, Robert F. McCue, Ian D. Voyles, Paul M. Robertson, Ian Li, Ling Chi, Miaofang Destino, Joel F. Devaraj, Arun Marquis, Emmanuelle A. Segre, Carlo U. Liu, Huinan H. Yang, Judith C. Momeni, Kasra Misra, Amit Abdolrahim, Niaz Medvedeva, Julia E. Cai, Wenjun Sehirlioglu, Alp Dizbay-Onat, Melike Mehta, Apurva Graham-Brady, Lori Maruyama, Benji Rajan, Krishna Warner, Jamie H. Taheri, Mitra L. Kalinin, Sergei V. Reeja-Jayan, B. Schwarz, Udo D. Simon, Sindee L. Brown, Craig M. Department of Chemistry and Department of Materials Science & Engineering Texas A & M University College Station United States Pritzker School of Molecular Engineering The University of Chicago Chicago United States Department of Materials Science & Engineering University of California Berkeley Berkeley United States National Center for Electron Microscopy Molecular Foundry Lawrence Berkeley National Laboratory Berkeley United States Center for Nanoscale Materials Argonne National Laboratory Lemont United States Department of Physics University of Washington Seattle United States Department of Materials Science & Engineering The Ohio State University Columbus United States Department of Mechanical Engineering and Mechanics Drexel University Philadelphia United States School of Mechanical Engineering Purdue University West Lafayette United States Department of Chemistry University of Southern California Los Angeles United States Department of Chemistry Stony Brook University The State University of New York Stony Brook United States Department of Chemistry University of Kentucky Lexington United States Department of Physics University of Illinois at Chicago Chicago United States Department of Materials Science & Engineering Northwestern University Evanston Bangladesh Department of Materials Science & Engineering University of Wisconsin–Madison Madison United States Department of Materials Science and Engineering University of Pennsylvania Philadelphia United States Department of Mechanical Engineering and Materials Science Duke University Durham United States Department of Chemistry Creighton University Omaha United States Physical and Computational Sciences Directorate Pacific Northwest National Laboratory Richland United States Department of Materials Science & Engineering University of Michigan–Ann Arbor Ann Arbor United States Department of Physics Illinois Institute of Technology Chicago United States Department of Bioengineering and the Mater

This article was updated to correct Minghao Zhang’s affiliation from "Pritzker School of molecular engineering, The University of Chicago, Chicago, USA" to "Pritzker School of molecular engineering, The University of Chicago, Chicago, USA;Argonne National Laboratory, Lemont, USA." Argonne National Laboratory was left off the original publication. © The Author(s) 2025.

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