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Diving into COVID-19: Visualizing SARS-CoV-2 Patient Proteins using Liquid-Electron Microscopy

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 2023年第1期29卷 662-664页

作者： Berry, Samantha DiCecco, Liza-Anastasia Gray, Jennifer Boylan, Jack Solares, Maria Kelly, Deborah F. Department of Biomedical Engineering Pennsylvania State University University Park PA United States Department of Materials Science and Engineering McMaster University Hamilton ON Canada Materials Research Institute Pennsylvania State University University Park PA United States Molecular Cellular Integrative Biosciences Graduate Program Huck Institutes of the Life Sciences Pennsylvania State University University Park PA United States Huck Institutes of the Life Sciences Pennsylvania State University University Park PA United States

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Using 4D-STEM to Map 3D Morphologies of 2D Materials

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Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 2023年第1期29卷 262-263页

作者： Mireles, Adan Shi, Chuqiao Park, Jeongwon Shin, Bongki Sung, Suk Hyun Ophus, Colin Hovden, Robert Kang, Kibum Han, Yimo Applied Physics Graduate Program Smalley-Curl Institute Rice University Houston TX United States Department of Materials Science and NanoEngineering Rice University Houston TX United States Department of Materials Science and Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon South Korea Department of Materials Science and Engineering University of Michigan Ann Arbor MI United States National Center for Electron Microscopy Molecular Foundry Lawrence Berkeley National Laboratory Berkeley CA United States

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Imaging atomic-scale chemistry from fused multi-modal electron microscopy

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npj Computational Materials 2022年第1期8卷 164-171页

作者： Jonathan Schwartz Zichao Wendy Di Yi Jiang Alyssa J.Fielitz Don-Hyung Ha Sanjaya D.Perera Ismail El Baggari Richard D.Robinson Jeffrey A.Fessler Colin Ophus Steve Rozeveld Robert Hovden Department of Materials Science and Engineering University of MichiganAnn ArborMIUSA Mathematics and Computer Science Division Argonne National LaboratoryLemontILUSA Advanced Photon Source Facility Argonne National LaboratoryLemontILUSA Dow Chemical Co. MidlandMIUSA Department of Material Science and Engineering Cornell UniversityIthacaNew YorkUSA School of Integrative Engineering Chung-Ang UniversitySeoulRepublic of Korea Department of Physics Cornell UniversityIthacaNYUSA The Rowland Institute at Harvard CambridgeMAUSA Department of Electrical Engineering and Computer Science University of MichiganAnn ArborMIUSA National Center for Electron Microscopy Lawrence Berkeley National LaboratoryMolecular FoundryBerkeleyCAUSA Applied Physics Program University of MIchiganAnn ArborMIUnited States

Efforts to map atomic-scale chemistry at low doses with minimal noise using electron microscopes are fundamentally limited by inelastic ***,fused multi-modal electron microscopy offers high signal-to-noise ratio(SNR)recovery of material chemistry at nano-and atomic-resolution by coupling correlated information encoded within both elastic scattering(high-angle annular dark-field(HAADF))and inelastic spectroscopic signals(electron energy loss(EELS)or energy-dispersive x-ray(EDX)).By linking these simultaneously acquired signals,or modalities,the chemical distribution within nanomaterials can be imaged at significantly lower doses with existing detector *** many cases,the dose requirements can be reduced by over one order of *** high SNR recovery of chemistry is tested against simulated and experimental atomic resolution data of heterogeneous nanomaterials.

关键词： materials chemistry fused

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Steady-state phases and interaction-induced depletion in a driven-dissipative chirally-coupled dissimilar atomic array

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Physical Review Research 2024年第2期6卷 023232-023232页

作者： Shao-Hong Chung I Gusti Ngurah Yudi Handayana Yi-Lin Tsao Chun-Chi Wu G.-D. Lin H. H. Jen Department of Physics and Center for Quantum Science and Engineering Taipei 10617 Taiwan Molecular Science and Technology Program Taiwan International Graduate Program Department of Physics Physics Division National Center for Theoretical Sciences Taipei 10617 Taiwan Trapped-Ion Quantum Computing Laboratory Hon Hai Research Institute Taipei 11492 Taiwan

A nanophotonic waveguide coupled with an atomic array forms one of the strongly coupled quantum interfaces to showcase many fascinating collective features of quantum dynamics. In particular, for a dissimilar array of two different interparticle spacings with competing photon-mediated dipole-dipole interactions and directionality of couplings, we study the steady-state phases of atomic excitations under a weakly driven condition of laser field. We identify a partial set of steady-state phases of the driven system composed of combinations of steady-state solutions in a homogeneous array. We also reveal the intricate role of the atom at the interface of the dissimilar array in determining the steady-state phases and find an alteration in the dichotomy of the phases strongly associated with steady-state distributions with crystalline orders. We further investigate in detail the interaction-induced depletion in half of the dissimilar array, where the blockaded region results from two contrasting interparticle spacings near the reciprocal coupling regime. This can be further evidenced from the analytical solutions under the reciprocal coupling. Our results can provide insights in the driven-dissipative quantum phases of atomic excitations with nonreciprocal couplings and pave the avenues toward quantum simulations of exotic many-body states essential for quantum information applications.

关键词： Collective behavior Dissipative dynamics Nanophotonics Phase diagrams Dipole approximation Two-level models

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Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a molecular NIR-II Cell Tracker

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Research 2021年第1期2021卷 935-950页

作者： Hao Chen Huaxiao Yang Chen Zhang Si Chen Xin Zhao Mark Zhu Zhiming Wang Yuebing Wang Hung-Ta Wo Kai Li Zhen Cheng Center for Molecular Imaging Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Molecular Imaging Program at Stanford(MIPS) Bio-X Programand Department of RadiologyCanary Center at Stanford for Cancer Early DetectionStanford UniversityStanfordCA 94305-5344USA University of North Texas Biomedical EngineeringDentonTX 76207USA Department of Biomedical Engineering Southern University of Science and TechnologyShenzhenGuangdong 518055China Department of Neurology Xiangya HospitalCentral South UniversityXiangya Road 88ChangshaHunan 410008China Stanford Cardiovascular Institute StanfordCA 94305USA Division of Cardiology Department of Internal MedicineChang Gung Memorial HospitalLinkouTaiwan33305China

Stem cell therapy holds high promises in regenerative *** major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution,migration,and engraftment,which cannot be easily achieved by current *** address this issue,for the first time,we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared(NIR-II)window(1,000-1,700 nm)for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal *** NIR-II tracker(CelTrac1000)has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem *** advantage of the unique merits of CelTrac1000,the responses of transplanted stem cells to different diseased environments have been discriminated and ***,we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100μm single-cell cluster spatial resolution,along with the lung contraction and heart *** such,this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy,affording optimal doses and efficacy.

关键词： doses distribution holds

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A Multi-Scale Approach to Assess Water Quality and Evaporation in Agricultural Irrigation Canals

SSRN

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SSRN 2024年

作者： Fender, Chloe L. Good, Stephen Garcia-Jaramillo, Manuel Department of Environmental and Molecular Toxicology Oregon State University CorvallisOR United States Department of Biological and Ecological Engineering Oregon State University CorvallisOR United States Water Resources Graduate Program Oregon State University CorvallisOR United States

Irrigation networks play a crucial role in water distribution and food production in agricultural systems and contribute to the overall economy. While studies have examined the effects of organic chemical pollutants, including pesticides, on crop and soil health, there is a lack of research on the overall composition and occurrence of organic chemical contaminants in irrigation water. To this point, there are currently no standardized methods for monitoring contaminants and assessing the transformation and potential toxicity of these compounds in irrigation water distribution systems. In this study, we used liquid chromatography high-resolution mass spectrometry to analyze the organic chemical composition of water samples from an irrigation canal and a river that serves as the source of water for the canal. We estimated the relative evaporation rate in the canal as a proxy for residence time by measuring the isotopic ratio of hydrogen and oxygen in the canal water with cavity ring-down spectroscopy. We assessed adverse effects of chemical contaminants in our samples by evaluating the collected water with a high-throughput zebrafish embryonic platform. We evaluated the hazard of annotated chemicals with an assessment tool developed by the US EPA. The canal showed a greater decrease in the abundance of organic contaminants relative to the river as we moved further from the source. Evaporation, a proxy for residence time, was evaluated in the canal and increased after a holding reservoir due to its higher surface area relative to the rest of the canal. This study improves our understanding of the occurrence and degradation of organic chemicals in irrigation networks compared with rivers, and how they may correlate with evaporation and distance. The approach implemented in this study can help build a standardized methodology for monitoring water quality and assessing the potential toxicity of organic contaminants in agricultural irrigation systems. © 2024, The Authors.

关键词： Organic chemicals

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Quantitative Imaging of 55CO and 18F-Labeled Tracers in a Single “Multiplexed” Pet Imaging Session

Quantitative Imaging of 55CO and 18F-Labeled Tracers in a Si...

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IEEE International Symposium on Biomedical Imaging

作者： Sarah J. Zou Irene Lim Jackson W. Foster Garry Chinn Hailey A. Houson Suzanne E. Lapi Jianghong Rao Craig S. Levin Department of Electrical Engineering Stanford University Department of Radiology and Molecular Imaging Program at Stanford (MIPS) Stanford University Chemistry and Biochemistry Department Bates College Department of Radiology University of Alabama at Birmingham Department of Bioengineering Stanford University Department of Physics Stanford University

ISBN: (数字)9798331520526

ISBN: (纸本)9798331520533

In this study, we explore the use of Cobalt-55 as a radioisotope for multiplexed PET (mPET) by utilizing its emission of a prompt gamma-ray in cascade with a positron during decay. We leverage the prompt-gamma signal to generate triple coincidences for a Co-55-labeled tracer, allowing us to distinguish it from a tracer labeled with a pure positron emitter F-18, which yields a two photon coincidence. By employing this triple versus double coincidence detection and signal processing methodology, we show the potential to separate and quantify the Co-55 signal from that of F-18. Phantom studies showed strong linear correlation $(R^{2}=0.99)$ between Co-55 double and triple coincidence counts and total Co-55 activity. Finally, we highlight the capability of this method to quantify overlapping and non-overlapping hot spots within a warm background to within a 3 to 15% error range. Moreover, we demonstrate the feasibility of simultaneously imaging two tracers in vivo during a single PET session using mice.

关键词： Multiplexing Sensitivity Translation Positrons Biomarkers Recording Labeling Positron emission tomography Isotopes Cancer

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Atomic excitation delocalization at the clean to disordered interface in a chirally-coupled atomic array

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Physical Review Research 2024年第1期6卷 013159-013159页

作者： C.-C. Wu K.-T. Lin I. G. N. Y. Handayana C.-H. Chien S. Goswami G.-D. Lin Y.-C. Chen H. H. Jen Institute of Atomic and Molecular Sciences Academia Sinica Taipei 10617 Taiwan Department of Physics and Center for Quantum Science and Engineering National Taiwan University Taipei 10617 Taiwan Molecular Science and Technology Program Taiwan International Graduate Program Academia Sinica Taipei 115201 Taiwan Department of Physics National Central University Taoyuan City 320317 Taiwan Physics Division National Center for Theoretical Sciences Taipei 10617 Taiwan Trapped-Ion Quantum Computing Laboratory Hon Hai Research Institute Taipei 11492 Taiwan

In one-dimensional quantum emitter systems, the dynamics of atomic excitations are influenced by the collective coupling between emitters through photon-mediated dipole-dipole interactions. By introducing positional disorders in a portion of the atomic array, we investigate the delocalization phenomena at the interface between the disordered and clean zones. The excitation is initialized as symmetric Dicke states in the disordered zone, and several measures are used to quantify the excitation localization. We first use population imbalance and half-chain entropy to investigate the excitation dynamics under time evolutions, and further investigate the crossover of excitation localization to delocalization via the gap ratio from the eigenspectrum in the reciprocal coupling case. In particular, we study the participation ratio of the whole chain and the photon loss ratio between both ends of the atomic chain, which can be used to quantify the delocalization crossover in the nonreciprocal coupling cases. Furthermore, by increasing the overall size or the ratio of the disordered zone under a fixed number of the whole chain, we observe that excitation localization occurs at a smaller disorder strength in the former case, while in the latter, facilitation of the delocalization appears when a significant ratio of the clean zone to disordered zone is applied. Our results can reveal the competition between the clean zone and the disordered zone sizes on localization phenomenon, give insights to nonequilibrium dynamics in the emitter-waveguide interface, and provide potential applications in quantum information processing.

关键词： Localization Collective dynamics Disordered systems

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Passive elasticity properties of Octopus rubescens arm

arXiv

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arXiv 2023年

作者： Halder, Udit Gribkova, Ekaterina Gillette, Rhanor Mehta, Prashant G. Coordinated Science Laboratory United States Neuroscience Program United States Department of Molecular and Integrative Physiology Department of Mechanical Science and Engineering University of Illinois Urbana-ChampaignIL61801 United States

In this report, passive elasticity properties of Octopus rubescens arm tissue are investigated using a multidisciplinary approach encompassing biomechanical experiments, computational modeling, and analyses. Tensile tests are conducted to obtain stress-strain relationships of the arm under axial stretch. Rheological tests are also performed to probe into dynamic shear response of the arm tissue. Based on these tests, comparisons against three different viscoelasticity models are reported. © 2023, CC BY-NC-ND.

关键词： Tissue

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An Open Hardware Bioprinter Part I: A bioextruder with a peristaltic pump approach 30

An Open Hardware Bioprinter Part I: A bioextruder with a per...

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30th IEEE International Conference on Electronics, Electrical engineering and Computing, INTERCON 2023

作者： Latorre-Quevedo, Piero G. Romero-Rodriguez, Omar A. Ramos-Gallegos, Mauricio L. Bravo-Bravo, Ashley E. Zimic-Sheen, Alen Torres-Ayala, Lizardo K. Castillo, Denis Biomedical Engineering Program PUCP-UPCH Pontificia Universidad Catolica Del Peru Lima Peru Biomedical Engineering Program PUCP-UPCH Universidad Peruana Cayetano Heredia Lima Peru Biology Program Universidad Peruana Cayetano Heredia Lima Peru Ambiental Engineering Program Universidad Peruana Cayetano Heredia Lima Peru Laboratorio de Ingeniería Biomédica Laboratorios de Investigación y Desarrollo Facultad de Ciencias e Ingeniería Universidad Peruana Cayetano Heredia Av Honorio Delgado 430 SMP Lima15102 Peru Laboratorio de Bioinformática Biología Molecular y Desarrollos Tecnológicos Laboratorios de Investigación y Desarrollo Facultad de Cienciase Ingeniería Universidad Peruana Cayetano Heredia Av Honorio Delgado 430 SMP Lima15102 Peru Laboratorios de Investigación y Desarrollo Facultad de Ciencias e Ingeniería Universidad Peruana Cayetano Heredia Av Honorio Delgado 430 SMP Lima15102 Peru

ISBN: (纸本)9798350315578

This study presents the development and characterization of an open source bioextruder for 3D bioprinting, focusing on its application in research laboratories in South America. Bioextruder design is based on several requirements, the design was manufactured using techniques of 3D printing and its electronic control is managed using an Arduino Mega and Ramps 1.4 shield. The paper evaluates mechanical and biological properties, demonstrating that the flow behavior of alginate through the bioextruder is influenced by its concentration, viscosity, and extrusion rate. Results: As for the mechanical characterization test, for low alginate concentrations high velocities show stable extrusion values, while for high concentrations, a low velocity gives stable results. Strand thickness consistent results were obtained with respect to the width of the prints. It is also shown that cell viability decreases as the extrusion speed increases. Conclusion: This research demonstrates a successful bioextruder for precise 3D bioprinting, emphasizing open-source hardware's significance particularly in resource-limited regions like Latin America. © 2023 IEEE.

关键词： Tissue engineering

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