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Effectiveness of dietary heat-killed Bacillus subtilis harboring plasmid containing 60 copies of CpG-ODN 1668 against Vibrio harveyi in Penaeus vannamei

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Veterinary Research Communications 2024年第1期48卷 85-101页

作者： Purbiantoro, Wahyu Huynh-Phuoc, Vinh Castillo-Corea, B.R.J. Byadgi, Omkar Vijay Cheng, Ta-Chih Laboratory of Molecular Fish Immunology and Genetics Department of Tropical Agriculture and International Cooperation National Pingtung University of Science and Technology Pingtung Taiwan Research Center for Animal Biologics National Pingtung University of Science and Technology Pingtung Taiwan Research Center for Marine and Land Bioindustry National Research and Innovation Agency (BRIN) Nusa Tenggara Barat Mataram Indonesia College of Aquaculture and Fisheries Can Tho University Can Tho Viet Nam Department of Biotechnology and Bioindustry Sciences National Cheng Kung University Tainan Taiwan International Program in Ornamental Fish Technology and Aquatic Animal Health International College National Pingtung University of Science and Technology Pingtung Taiwan

The cost of the purification process hinders the extensive use of cytosine phosphate guanosine-oligodeoxynucleotides (CpG-ODNs) for shrimp culture. Therefore, this study used a shuttle vector plasmid to carry 60 copies of CpG-ODN 1668 (pAD43-25_60CpG), which can replicate in Escherichia coli and Bacillus subtilis strain RIK1285. The first experiment used a reverse gavage procedure to deliver a substance (PBS [CK], pAD43-25 [P0], and pAD43-25_60CpG [P60], respectively) directly into the anterior midgut of Penaeus vannamei and transcriptome sequence analysis with a reference genome was performed to examine the expression of well-known immune-related genes. The results showed that the expression levels of immune-related genes in P60 group were significantly increased, particularly those associated with AMPs. In addition, using RT‒qPCR, the expression levels of AMP genes (LvALF, LvPEN-2, and LvPEN-3) in the P60 group may vary depending on the tissue and time point. The second experiment used dietary supplementation with three kinds of heat-killed B. subtilis (HKBS, HKBS-P0, and HKBS-P60) in 28 days of feeding experiments. The results showed that dietary supplementation with HKBS-P60 did not significantly improve shrimp growth performance and survival. However, on days 14 and 28 of the feeding regimens, alkaline phosphatase (AKP) and acid phosphatase (ACP) activity were considerably higher than in other treatments. In addition, following infection with Vibrio harveyi, AKP and ACP activity in the HKBS-P60 group was significantly higher than in other treatments, particularly at the early stage of bacterial infection. Moreover, HKBS-P60 was found to be better protected against V. harveyi infection with lower cumulative mortality (60%) compared to HKBS (90%) and HKBS-P0 (100%) at 7 days after infection. Overall, these findings confirmed that P60 could increase immunological responses in the shrimp midgut, and HKBS-P60 could be used as an effective tool to enhance the immune

关键词： Acid phosphatase Alkaline phosphatase Antimicrobial peptides Heat-killed Bacillus subtilis Multiple copies of CpG-ODN 1668 Oral administration Penaeus vannamei

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Near-infrared benzodiazoles as small molecule environmentally-sensitive fluorophores

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Frontiers of Chemical science and Engineering 2022年第1期16卷 128-135页

作者： Fabio de Moliner Ina Biazruchka Karolina Konsewicz Sam Benson Suraj Singh Jun-Seok Lee Marc Vendrell Centre for Inflammation Research Queen’s Medical Research InstituteThe University of EdinburghEdinburgh EH164TJUK Molecular Recognition Research Centre Korea Institute of Science and Technology(KIST)&Bio-Med ProgramKIST-School USTSeoul 02792Korea Department of Pharmacology Korea University College of MedicineSeoul 02841Korea

The development of fluorophores emitting in the near-infrared spectral window has gained increased attention given their suitable features for biological *** this work,we have optimised a general and straightforward synthetic approach to prepare a small library of near-infrared-emitting C-bridged nitrobenzodiazoles using commercial precursors.C-bridged benzodiazoles have low molecular weight and neutral character as important features that are not common in most nearinfrared *** have investigated their fluorescence response in the presence of a wide array of 60 different biomolecules and identified compound 3i as a potential chemosensor to discriminate between Fe2+and Fe^(3+)ions in aqueous media.

关键词： fluorescence probes iron screening library

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Direct Identification of Intact Proteins Using a Low-Resolution Mass Spectrometer with CIDn/ETnoD

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Journal of the American Society for Mass Spectrometry 2024年第7期35卷 1507-1515页

作者： Kuo, Cheng-Yu Zheng, Yi-Feng Wang, Wei-Chen Toh, Jie-Teng Hsu, Yu-Ming Chien, Han-Ju Chang, Chih-Jui Lai, Chien-Chen Institute of Molecular Biology National Chung Hsing University Taichung402 Taiwan Department of Biochemical Science and Technology National Chiayi University Chiayi600 Taiwan Department of Molecular Biology and Human Genetics Tzu Chi University Hualien City970 Taiwan Advanced Plant and Food Crop Biotechnology Center National Chung Hsing University Taichung402 Taiwan Graduate Institute of Chinese Medical Science China Medical University Taichung406 Taiwan Doctoral Program in Translational Medicine National Chung Hsing University Taichung402 Taiwan Rong Hsing Translational Medicine Research Center National Chung Hsing University Taichung402 Taiwan

Over the past decades, proteomics has become increasingly important and a heavily discussed topic. The identification of intact proteins remains a major focus in this field. While most intact proteins are analyzed using high-resolution mass spectrometry, identifying them through low-resolution mass spectrometry continues to pose challenges. In our study, we investigated the capability of identifying various intact proteins using collision-induced dissociation (CID) and electron transfer without dissociation (ETnoD). Using myoglobin as our test protein, stable product ions were generated with CID, and the identities of the product ions were identified with ETnoD. ETnoD uses a short activation time (AcT, 5 ms) to create sequential charge-reduced precursor ion (CRI). The charges of the fragments and their sequences were determined with corresponding CRI. The product ions can be selected for subsequent CID (termed CIDn) combined with ETnoD for further sequence identification and validation. We refer to this method as CIDn/ETnoD. The use of a multistage CID activation (CIDn) and ETnoD protocol has been applied to several intact proteins to obtain multiple sequence identifications. © 2024 The Authors. Published by American Chemical Society.

关键词： Dissociation

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Rational effect of CuSe2phase with octadecyl amine modified reduced graphene composite for dual-mode sensing of environmental hazardous anthelminthic drug in aquatic and biological samples

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Journal of Environmental Chemical Engineering 2023年第1期11卷

作者： Hwa, Kuo-Yuan Ganguly, Anindita Santhan, Aravindan Department of Molecular Science and Engineering National Taipei University of Technology Taipei Taiwan International Graduate Program in Energy and Optoelectronic Materials National Taipei University of Technology Taipei Taiwan

The increasing usage of antihelminthic drug mebendazole (MEB) in humans and livestock poses serious threat and is considered a significant environmental pollutant. Hence, monitoring the MEB residues in environmental samples, and human fluids is essential to decrease the ecological risk and improve the health of humans. In this work, copper selenide (CuSe2) nano/microspheres were hydrothermally synthesized and ultrasonically treated with rGO surface modified with ODA alkyl amine (rGO-ODA). The optical, structural, morphological, and functional analysis were performed which revealed the CuSe2 in marcasite phase, orthorhombic pattern with Pnnm space group, and substantial composite formation. The morphological studies revealed sphere structure of the CuSe2 and wrinkled sheets of rGO-ODA. A sensor that detects MEB using the cyclic voltammetry (CV), differential pulse voltammetry (DPV) and UV-Vis absorbance techniques was developed. For DPV analysis, the sensor exhibited a very low value for limit of detection 13.55 nM and a sensitivity of 9.4871 μA M-1cm-2. For UV-Vis, the sensor demonstrated a very low limit of detection 22.3 nM. The use of this sensor was validated in real-time analysis with aquatic and biological real samples. The results suggested > 90% of analyte recovery and enhanced efficiency for MEB detection with a potential for commercialization in future. © 2022 Elsevier Ltd.

关键词： Copper compounds

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New type of frequency standard transitions realized by comb laser

New type of frequency standard transitions realized by comb ...

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CLEO: science and Innovations, S and I 2022

作者： Chen, Bo-Wei Liu, Tze-Wei Yu, Hsin-Hung Cheng, Wang-Yau Department of Physics National Central University Taoyuan32001 Taiwan Molecular Sciences and Technology Taiwan International Graduate Program Academia Sinica National Central University Taipei Taiwan Institute of Atomic and Molecular Science Academia Sinica Taipei11529 Taiwan

The clock transitions recommended by BIPM are never realized by a femtosecond comb laser. In this report, we recommend two comb-laser-based stepwise transitions resolved simultaneously from rubidium and cesium atom. &... 详细信息

ISBN: (纸本)9781557528209

关键词： Frequency standards

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Effective monitoring of environmental hazardous drug moxifloxacin hydrochloride using silver selenide on octadecyl amine modified reduced graphene oxide composite

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Journal of Environmental Chemical Engineering 2024年第1期12卷

作者： Hwa, Kuo-Yuan Ganguly, Anindita Graduate Institute of Organic and Polymeric Materials National Taipei University of Technology Taipei China Department of Molecular Science and Engineering National Taipei University of Technology Taipei China International Graduate Program in Energy and Optoelectronic Materials National Taipei University of Technology China

The precise detection of antibiotics is very critical. Here, silver selenide (Ag2Se) nanorods are hydrothermally synthesized and ultrasonically treated with rGO surface modified with octadecylamine (rGO-ODA). The optical, structural, morphological, and functional analysis were performed which revealed the orthorhombic pattern of the P2221 E space group, the crystalline purity of Ag2Se, and the substantial composite formation. The morphological studies revealed nanorod like structure of the Ag2Se material and wrinkled sheets of rGO-ODA. A sensor that detects moxifloxacin hydrochloride (MOXH) antibiotic using the cyclic voltammetry (CV), differential pulse voltammetry (DPV) and UV-Vis absorbance was developed. For DPV analysis, a quite low value for detection limit 12.46 nM was exhibited with 0.199 μM – 425.16 μM linear range, and a sensitivity of 5.0439 μA M−1cm−2. For UV-Vis, the sensor demonstrated a very low limit of detection 17.42 nM over 0.12 μM – 7.7 μM working range. The use of this sensor was validated in real-time analysis with industrial waste water, fish, human blood, and urine samples. The results suggested an excellent rate of analyte recovery and enhanced efficiency for MOXH detection with a potential for commercialization in the future. © 2023

关键词： Nanorods

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Structure-Guided Discovery of PD-1/PD-L1 Interaction Inhibitors: Peptide Design, Screening, and Optimization via Computation-Aided Phage Display Engineering

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Journal of Chemical Information and Modeling 2024年第5期64卷 1615-1627页

作者： Tseng, Tien-Sheng Lee, Chao-Chang Chen, Po-Juei Lin, Chiu-Yuen Chen, Wang-Chuan Lee, Yu-Ching Lin, Jiun-Han Chen, Kaun-Wen Tsai, Keng-Chang Institute of Molecular Biology National Chung Hsing University Taichung40202 Taiwan National Research Institute of Chinese Medicine Ministry of Health and Welfare Taipei11221 Taiwan The School of Chinese Medicine for Post Baccalaureate I-Shou University Kaohsiung824005 Taiwan Department of Chinese Medicine E-Da Hospital Kaohsiung824005 Taiwan TMU Research Center of Cancer Translational Medicine Taipei Medical University Taipei11031 Taiwan Ph.D. Program in Medical Biotechnology College of Medical Science and Technology Taipei Medical University Taipei11031 Taiwan Department of Industrial Technology Ministry of Economic Affairs Taipei100210 Taiwan Food Industry Research and Development Institute Hsinchu30062 Taiwan Molecular Science and Digital Innovation Center Genetics Generation Advancement Corporation Taipei11949 Taiwan

Cancer immunotherapy harnesses the immune system to combat tumors and has emerged as a major cancer treatment modality. The PD-1/PD-L1 immune checkpoint modulates interactions between tumor cells and T cells and has been extensively targeted in cancer immunotherapy. However, the monoclonal antibodies known to target this immune checkpoint have considerable side effects, and novel PD-1/PD-L1 inhibitors are therefore required. Herein, a peptide inhibitor to disrupt PD-1/PD-L1 interactions was designed through structure-driven phage display engineering coupled to computational modification and optimization. BetaPb, a novel peptide library constructed by using the known structure of PD-1/PD-L, was used to develop inhibitors against the immune checkpoint, and specific peptides with high affinity toward PD-1 were screened through enzyme-linked immunosorbent assays, homogeneous time-resolved fluorescence, and biolayer interferometry. A potential inhibitor, B8, was preliminarily screened through biopanning. The binding affinity of B8 toward PD-1 was confirmed through computation-aided optimization. Assessment of B8 variants (B8.1, B8.2, B8.3, B8.4, and B8.5) demonstrated their attenuation of PD-1/PD-L1 interactions. B8.4 exhibited the strongest attenuation efficiency at a half-maximal effective concentration of 0.1 μM and the strongest binding affinity to PD-1 (equilibrium dissociation constant = 0.1 μM). B8.4 outperformed the known PD-1/PD-L1 interaction inhibitor PL120131 in disrupting PD-1/PD-L1 interactions, revealing that B8.4 has remarkable potential for modification to yield an antitumor agent. This study provides valuable information for the future development of peptide-based drugs, therapeutics, and immunotherapies for cancer. © 2024 American Chemical Society.

关键词： Peptides

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SiC structural characterization by non destructive near-field microscopy techniques

SiC structural characterization by non destructive near-fiel...

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2022 International Semiconductor Conference, CAS 2022

作者： Wu, Kuan-Ting Vuillermet, Enora Usureau, Elise El-Helou, Youssef Kazan, Michel Woon, Wei-Yen Lazar, Mihai Bruyant, Aurelien Light Nanomaterials and Nanotechnologies Cnrs Emr 7004 University of Technology of Troyes Troyes10004 France Department of Physics National Central University Taoyuan32001 Taiwan Molecular Science and Technology Program Taiwan International Graduate Program Institute of Atomic and Molecular Science Academia Sinica Taipei10617 Taiwan Department of Physics American University of Beirut P.O. Box 11-0236 Riad El-Solh Beirut1107-2020 Lebanon

ISBN: (数字)9781665452557

ISBN: (纸本)9781665452557

SiC sputtered and e-beam evaporated layers have been deposited on 4H-SiC substrates. Recrystallization has been tried by high temperature annealing with two plateaus at 1400°C and 1700°C. The crystallinity was systematically investigated by non destructive near-field microscopy techniques, particularly adapted to the thin thickness of our layers. Good recrystallization is confirmed only on the e-beam evaporated layers and after the higher annealing plateau by preserving 4H substrate polytype. © 2022 IEEE.

关键词： Silicon carbide

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Microfractures in TiO2Enhance the Photoelectrochemical Properties of Photosystem i Bio-Sensitized Solar Cells 5

Microfractures in TiO2Enhance the Photoelectrochemical Prope...

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5th IEEE International Conference on BioInspired Processing, BIP 2023

作者： Flores, Sebastian Castro Araya, Christopher Espinoza Segura, Fabricio Chaverri Pineda, Leslie W. Bruce, Barry D. Villarreal, Claudia C. Cartago Costa Rica Maestria Ingenieria en Dispositivos Medicos Cartago Costa Rica San Jose Costa Rica University of Tennessee Department of Biochemistry Cellular and Molecular Biology Program in Genome Science and Technology KnoxvilleTN United States San Jose Costa Rica

ISBN: (纸本)9798350330052

Climate change, caused by the increasing energy demand from fossil fuels due to population growth and economic expansion, triggers the need for clean, renewable, and sustainable energy sources. The solar energy contributes to approximately 99.9% of the Earth's energy. Therefore, photovoltaic technology is considered a possible substitute for fossil fuels owing to its abundance, but the current silicon and thin film photovoltaic (PV) technologies generate greenhouse gas emissions (GHE) during manufacturing and use scarce and polluting materials. Bio-sensitized solar cells (BSSCs) offer a promising solution using simple, lower energy-demand manufacturing processes and abundant, non-toxic materials. BSSCs are derived from dye-sensitized solar cells (DSSCs) technology by replacing the synthetic dye with biological molecules, such as photosystem I (PSI), into a thin film device. We report a BSSC using PSI as a sensitizer, TiO2 as a wide-bandgap semiconductor, Co-bipyridine compound as a redox mediator in the electrolyte, and PEDOT-CNT composite as the counter electrode. This study investigates how microfractures on the TiO2 thin film induced during thermal sintering affect the photoelectrochemical performance of the BSSCs. Electrochemical characterization of the PSI-BSSCs was performed under a solar simulator using linear sweep voltammetry (LSV), open-circuit voltage decay (OCVD), chronoamperometry, and electrochemical impedance spectroscopy (EIS). SEM micrographs reveal fissures in the photoanode surface induced by the fast heating and cooling during thermal treatments. Surprisingly, the microfractures improved the performance of the solar cells and will be discussed regarding the accessibility of PSI and the mediator to penetrate the nanostructured electrode and enhance the photocurrents generated. The BSSCs improvement due to the microfractures is ascribed to the enabled diffusion of the PSI sensitizer through the nanoporous TiO2, given the significantly larger size o

关键词： bio-sensitized solar cell microfractures photosystem I surface area TiO2 photoanodes

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Strongly Confined Atomic Excitation Localization in a Weakly-Driven Atom-Waveguide Interface

arXiv

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arXiv 2024年

作者： Chung, Shao-Hung Chen, Wei Jen, H.H. Institute of Atomic and Molecular Sciences Academia Sinica Taipei10617 Taiwan Department of Physics Center for Quantum Science and Engineering National Taiwan University Taipei10617 Taiwan Molecular Science and Technology Program Taiwan International Graduate Program Academia Sinica Taiwan Physics Division National Center for Theoretical Sciences Taipei10617 Taiwan

An atomic array coupled to a photonic crystal waveguide forms a strongly coupled quantum interface, exhibiting various intriguing collective features of quantum dynamics. Here we consider a homogeneous atomic array and theoretically investigate its steady-state distribution when the incident fields drive the atoms from both sides at asymmetric angles. This effectively creates an interface shared by two zones of atoms under different driving angles. This setup introduces a competition between photon-mediated dipole-dipole interactions and the directionality of coupling, while differences of the travelling phases from the incident angles further influence the overall steady-state behavior. Under this asymmetric driving scheme, the presence of strongly confined localization can be identified, where localization can occur either at the interface or at one of edges. Additionally, we examine the size effect on the atomic localization, deriving an empirical formula to predict parameter regimes that favor interfaced localization. We also consider a defect-driving scheme, where a third zone is created by undriven atoms under symmetric travelling phases. This results in strongly confined single-site excitation localization, which can be explained through analytical solutions under the reciprocal coupling. Finally, we propose several methods for precise control of multiple single-site localizations under the defect-driving scheme. Our results provide insights into driven-dissipative quantum systems with nonreciprocal couplings and pave the way for quantum simulation of exotic many-body states relevant to quantum information applications. Copyright © 2024, The Authors. All rights reserved.

关键词： Photonic crystals

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