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Scaling Up Synthetic Cell Production Using Robotics and Machine Learning Toward Therapeutic Applications

Advanced Biology 2025年第5期9卷 e2400671页

作者： Sharf-Pauker, Noga Galil, Ido Kfir, Omer Chen, Gal Menachem, Rotem Shklover, Jeny Schroeder, Avi Ackerman, Shanny The Louis Family Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies Department of Chemical Engineering Technion – Israel Institute of Technology Haifa32000 Israel The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology Technion – Israel Institute of Technology Haifa32000 Israel Faculty of Computer Science Technion – Israel Institute of Technology Haifa32000 Israel The Interdisciplinary Program for Biotechnology Technion – Israel Institute of Technology Haifa32000 Israel Cell Biology and Cancer Science Rappaport Faculty of Medicine Technion – Israel Institute of Technology Haifa32000 Israel

Synthetic cells (SCs), developed through bottom-up synthetic biology, hold great potential for biomedical applications, with the promise of replacing malfunctioning natural cells and treating diseases with spatiotemporal control. Currently, most SC synthesis and characterization processes are manual, limiting scalability and efficiency. In this study, an automated method is developed for large-scale production of protein-producing SCs for therapeutic applications. The optimized process, compatible with a robotic liquid handling system (LiHa), reduces production time by half. Additionally, incorporation of an automated tissue dissociator-based emulsification increases batch size 30-fold while preserving SC characteristics. To assess SC quality and protein synthesis, artificial intelligence (AI)-based image analysis is employed, allowing for automated, accurate and high-throughput SC characterization. Large-scale luciferase-expressing SCs from a single homogeneous batch are administered to mice, allowing for real-time monitoring of protein expression and reducing experimental variability. By troubleshooting several central steps in SC synthesis, it is demonstrated that automation and computerized quality control can significantly improve the process of SC synthesis for preclinical and clinical applications. © 2025 The Author(s). Advanced Biology published by Wiley-VCH GmbH.

关键词： AI artificial Intelligence automation machine learning robotics synthetic biology synthetic cells

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Impact of High-k Materials and Tube Numbers on CNFET Gate's Performances 5

Impact of High-k Materials and Tube Numbers on CNFET Gate's ...

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5th International Conference on Smart Systems and Inventive technology, ICSSIT 2023

作者： Yalung, Christofer Tantraviwat, Doldet Uatrongjit, Sermsak Yamwong, Wittawat Doctor of Philosophy Program in Nanoscience and Nanotechnology International Program/Interdisciplinary Center of Excellence in Materials Science and Technology Faculty of Science Chiang Mai University Chiang Mai Thailand Chiang Mai University Faculty of Engineering Department of Electrical Engineering Chiang Mai Thailand Chachoengsao Thailand

ISBN: (纸本)9781665474672

Carbon nanotube field effect transistor (CNFET) performance is primarily influenced by a variety of characteristics, such as nanotube diameter, operating voltage, pitch, the number of tubes, dielectric constant, and contact materials. In this study, the dielectric constant and the number of carbon nanotubes (CNTs) were emphasized to analyze the propagation delay and average power dissipation of basic logic gates. Zirconium dioxide (ZrO2), hafnium oxide (HfO2), and silicon dioxide (SiO2) were used as dielectric materials. In this experiment, the Stanford CNFET model was utilized, and variable factors such as the number of CNTs and the dielectric constant (Kox) accumulated on the nanotube gate's surface were diversified. The correlation coefficients of the delay, the average power, and the CNT numbers were also presented. Furthermore, the multi-objective genetic algorithm and the Rmethod were utilized to find the optimal Kox and the number of CNTs for minimizing the average dissipation power and the propagation delay of some logic gates designed with CNFET. For all the gates considered, the optimal number of CNTs is 4 while the dielectric constant is within the ranges of 19 to 24. © 2023 IEEE.

关键词： Carbon nanotubes

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Cell surface display:its application to biotechnology

Cell surface display:its application to biotechnology

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The 14th China-Japan-Korea Joint Symposium on Enzyme Engineering

作者： Chan Woo Park Joseph P.Park Haeshin Lee Seung Hwan Lee Interdisciplinary Program of Bioenergy and Biomaterials Graduate School Chonnam National University Graduate School of Nanoscience and Technology Korea Advanced Institute of Science and Technology

Cell surface display is a technique to express peptides or proteins on the surface of bacteria,fungi,or even mammalian cells by fusing them to various surface anchoring *** can be used a non-conventional method for immobilization of enzyme,which make this technology apply to the many *** now,several applications such as live vaccine,adsorbents,biosensors and whole cell

关键词： Cell surface display:its application to biotechnology

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Cell surface display of mussel inspired catecholamine-the preparation of sticky Escherichia coli

Cell surface display of mussel inspired catecholamine-the pr...

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The 14th China-Japan-Korea Joint Symposium on Enzyme Engineering

Cell surface display is a technique to display peptides or proteins on the surface of gram-negative and gram-positive bacteria,fungi,or even mammalian cells by appropriately fusing them to surface anchoring *** can be used a non-conventional method for immobilization of ***,we present a sticky *** which displayed catecholamine,a mussel-inspired adhesive moiety with catechol and amine functional group,was displayed on the cell surface using outer membrane protein

关键词： Cell surface display of mussel inspired catecholamine-the preparation of sticky Escherichia coli

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Detachable Microneedles With Crosslinked Hyaluronic Acid as Superficial Skin Filler

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Journal of Biomedical Materials Research Part A 2025年第6期113卷 e37933页

作者： Pattrawadee Toprangkobsin Apasinee Teepakakorn Supanan Ampawa Ahmad Efendi Benchaphorn Limcharoen Wijit Banlunara Pravit Asawanonda Supason Wanichwecharungruang Department of Chemistry Faculty of Science and Technology Phra Nakhon Si Ayutthaya Rajabhat University Ayutthaya Thailand Department of Chemistry Faculty of Science Chulalongkorn University Bangkok Thailand Mineed Technology Co. Ltd. Bangkok Thailand Interdisciplinary Program of Nanoscience and Technology Graduate School Chulalongkorn University Bangkok Thailand Department of Anatomy Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand Center of Excellence in Materials and Bio-Interfaces Chulalongkorn University Bangkok Thailand Department of Pathology Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand Department of Medicine Division of Dermatology Faculty of Medicine Chulalongkorn University Bangkok Thailand

Crosslinking gives hyaluronic acid (HA) the ability to resist digestion by hyaluronidase; thus, crosslinked hyaluronic acid (cHA) is currently the most popular soft tissue filler. Clinically trained physicians usually administer cHA to the desired site via injections. Injection into the upper dermis and epidermis layers of the skin is challenging, and cHA with a low level of crosslinking is employed to prevent the uneven look, but it comes with a shorter lifetime. We demonstrate here the synthesis of cHA and its simultaneous fabrication into cHA-detachable microneedles (cHA-DDMNs). The cHA-DDMN patch allowed for the deposition of cHA into the ex vivo porcine skin in 1 min. The non-crosslinked HA placed into the ex vivo porcine skin spread out evenly within 30 min after it was applied. In contrast, the deposited cHA demonstrated prolonged localization in the tissue. Furthermore, we demonstrated the sustained release of curcumin delivered by cHA both in vitro and ex vivo. In rats, the administration of cHA-DDMNs to the skin did not cause skin irritation and led to a significant increase in dermal thickness and collagen deposition.

关键词： crosslinked-hyaluronic acid filler microneedles superficial skin

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Influence of the electrodeposited co-fe alloys’ characteristics on their magnetic properties 2014

Influence of the electrodeposited co-fe alloys’ characteris...

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2014 International Conference on Advanced Materials, Structures and Mechanical Engineering, ICAMSME 2014

作者： Chotibhawaris, Thanakrit Luangvaranunt, Tachai Jantaratana, Pongsakorn Boonyongmaneerat, Yuttanant Nanoscience and Technology Interdisciplinary Program Graduate School Chulalongkorn University Phyathai Rd Pathumwan Bangkok Thailand Metallurgical Engineering Department Chulalongkorn University Phyathai Rd Pathumwan Bangkok Thailand Department of Physics Kasetsart University Ngam Wong Wan Rd Chatuchak Bangkok Thailand Chulalongkorn University Phyathai Rd Pathumwan Bangkok Thailand

ISBN: (纸本)9783038352549

Nanocrystalline CoFe films were fabricated by electrodeposition process for an investigation of the relationship between the alloys’ characteristics and their magnetic properties. The study shows that coating thickness promotes softer magnetic properties of the films, and induces changes of film roughness, preferred orientation and domain pattern. The preferred orientation of the ~1 μm films (thin films) is (110) plane, whereas that of the ~3 μm films (thick films) are (110) and (200) planes. The magnetic domain of the thin films exhibit a stripe-liked pattern, whereas a bubble-liked pattern appears in the thick films. Iron content significantly affects the magnetic properties of the thick films. In this study, the 57.3wt.%Fe thick film has the highest saturation magnetization, and the 80.0wt.%Fe thick film shows the lowest coercivity. © (2014) Trans Tech Publications, Switzerland.

关键词： Electrodeposition

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Design and Fabrication of Two-Dimensional Photonic Crystals with Predetermined Nonlinear Optical Properties

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Physical Review Letters 2005年第10期94卷 103904-103904页

作者： Shin-ichiro Inoue Yoshinobu Aoyagi Nanoscience Research Program RIKEN (The Institute of Physical and Chemical Research) 2-1 Hirosawa Wako-shi Saitama 351-0198 Japan Interdisciplinary Graduate School of Science and Engineering Tokyo Institute of Technology 4259 Nagatsuta Midori-ku Yokohama 226-8502 Japan

By probing the resonances between a photonic band and an external laser field and their nonlinear changes in angle-resolved reflectivity, we show experimental evidence that the nonlinear optical changes in a two-dimensional photonic crystal waveguide with a Kerr nonlinearity are critically dependent on the dispersion nature and the group velocity of the photonic bands. The results agree well with the behavior predicted from band structures, indicating that the design of nonlinear optical properties of material systems is realistically possible by band dispersion and group velocity engineering.

关键词： Crystals

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Single-molecule fluorescence study on the phase shift and phase locking of RecA nucleoprotein filament

Single-molecule fluorescence study on the phase shift and ph...

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第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Sung Hyun Kim TakKyoon Ahn Chirlmin Joo Doseok Kim Interdisciplinary Program of Integrated Biotechnology Sogang UniversitySeoulRepublic of Korea Department of Physics Sogang UniversitySeoulRepublic of Korea Kavli Institute of NanoScience and Department of BioNanoScience Delft University of TechnologyDelftThe Netherlands

RecA protein lies at the core of DNA repair *** forms a helical structured filament on as single-strand DNA(ssDNA) appears as an intermediate of homologous *** the recent RecA crystal structure with embedded ssDNA,a RecA monomer binds to a group of three nucleotides and generates a gap in ***,there are three possible phases in the binding of RecA according to the relative position

关键词： RecA single molecule FRET DNA repair

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Influence of structural parameters on electrical characteristics of schottky tunneling field-effect transistor and its scalability

Influence of structural parameters on electrical characteris...

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作者： Wu, Yan Dou, Chunmeng Wei, Feng Kakushima, Kuniyuki Ohmori, Kenji Ahmet, Parhat Watanabe, Takanobu Tsutsui, Kazuo Nishiyama, Akira Sugii, Nobuyuki Natori, Kenji Yamada, Keisaku Kataoka, Yoshinori Hattori, Takeo Iwai, Hiroshi Frontier Research Center Tokyo Institute of Technology Yokohama 226-8502 Japan Interdisciplinary Graduate School of Science and Engineering Tokyo Institute of Technology Yokohama 226-8502 Japan Honors Graduate Program for Nanotech/Sciences University of Tsukuba Tsukuba Ibaraki 305-8573 Japan Graduate School of Pure and Applied Sciences University of Tsukuba Tsukuba Ibaraki 305-8573 Japan Institute for Nanoscience and Nanotechnology Waseda University Shinjuku Tokyo 169-8555 Japan

The influence of structural parameters, including the Schottky barrier height for electron (Bn) and channel doping (Na), on the electrical characteristics of a scaled Schottky barrier tunneling FET (SBTFET) have been clarified by numerical device simulation. The thermionic emission current (ITH) as well as the tunneling current (ITN) have been considered as the main electron injections at the source edge. Simulation results have revealed that the main conduction is ITN in the region near and above the threshold voltage (Vth). As tunneling probability is determined by Bn and the width of the triangular potential barrier at the source edge, a lower Bn with higher Na results in a better subthreshold swing (SS) with high on-state drive current (ION) at a gate length (Lg) of 50 nm. With Lg scaling down to 10 nm, however, a lower Bn has shown an increased offstate leakage current (IOFF) due to the short-channel effect (SCE), while a larger Bn can suppress the IOFF at the cost of ION. Therefore, considering SS with ION and IOFF ratio, it can be concluded that an optimum Bn exists for short-channel devices. The SBTFET showed good subthreshold performance and higher ION=IOFF than the conventional silicon-on-insulator (SOI) MOSFET in 10nm region with the Schottky barrier height optimization. © 2013 The Japan Society of Applied Physics.

关键词： Threshold voltage

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Influence of the Electrodeposited Co-Fe Alloys' Characteristics on their Magnetic Properties

Influence of the Electrodeposited Co-Fe Alloys' Characterist...

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The 2014 International Conference on Advanced Materials,Structures and Mechanical Engineering(ICAMSME 2014)

作者： Thanakrit Chotibhawaris Tachai Luangvaranunt Pongsakorn Jantaratana Yuttanant Boonyongmaneerat Nanoscience and Technology Interdisciplinary Program Graduate School Chulalongkorn University Metallurgical Engineering Department Faculty of Engineering Chulalongkorn University Department of Physics Faculty of Science Kasetsart University Metallurgy and Materials Science Research Institute (MMRI) Chulalongkorn University

Nanocrystalline CoFe films were fabricated by electrodeposition process for an investigation of the relationship between the alloys' characteristics and their magnetic properties. The study shows that coating thickness promotes softer magnetic properties of the films, and induces changes of film roughness, preferred orientation and domain pattern. The preferred orientation of the μm films（thin films） is（110） plane, whereas that of the μm films（thick films） are（110） and（200） planes. The magnetic domain of the thin films exhibit a stripe-liked pattern, whereas a bubble-liked pattern appears in the thick films. Iron content significantly affects the magnetic properties of the thick films. In this study, the 57.3wt.%Fe thick film has the highest saturation magnetization, and the 80.0wt.%Fe thick film shows the lowest coercivity.

关键词： Electrodeposition plating CoFe film magnetic properties

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