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Author Correction: Predicting the efficiency of prime editing guide RNAs in human cells

Nature biotechnology 2024年第3期42卷 529页

作者： Hui Kwon Kim Goosang Yu Jinman Park Seonwoo Min Sungtae Lee Sungroh Yoon Hyongbum Henry Kim Department of Pharmacology Yonsei University College of Medicine Seoul Republic of Korea. Brain Korea 21 Plus Project for Medical Sciences Yonsei University College of Medicine Seoul Republic of Korea. Center for Nanomedicine Institute for Basic Science (IBS) Seoul Republic of Korea. Graduate Program of Nano Biomedical Engineering (NanoBME) Advanced Science Institute Yonsei University Seoul Republic of Korea. Electrical and Computer Engineering Seoul National University Seoul Republic of Korea. Interdisciplinary Program in Bioinformatics Seoul National University Seoul Republic of Korea. Graduate School of Data Science Seoul National University Seoul Republic of Korea. Department of Pharmacology Yonsei University College of Medicine Seoul Republic of Korea. hkim1@yuhs.ac. Brain Korea 21 Plus Project for Medical Sciences Yonsei University College of Medicine Seoul Republic of Korea. hkim1@yuhs.ac. Center for Nanomedicine Institute for Basic Science (IBS) Seoul Republic of Korea. hkim1@yuhs.ac. Graduate Program of Nano Biomedical Engineering (NanoBME) Advanced Science Institute Yonsei University Seoul Republic of Korea. hkim1@yuhs.ac. Severance Biomedical Science Institute Yonsei University College of Medicine Seoul Republic of Korea. hkim1@yuhs.ac. Graduate Program of NanoScience and Technology Yonsei University Seoul Republic of Korea. hkim1@yuhs.ac.

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[email protected] nanocomposite modified electrode for sensitive and simultaneous detection of Cd (II) and Pb (II)

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Synthetic Metals 2018年 245卷 251-259页

作者： Jutiporn Yukird Pongsakorn Kongsittikul Jiaqian Qin Orawon Chailapakul Nadnudda Rodthongkum Nanoscience and Technology Interdisciplinary Program Graduate School Chulalongkorn University Phayathai Road Patumwan Bangkok 10330 Thailand Petrochemical and Polymer Science Faculty of Science Chulalongkorn UniversityPhayathai Road Pathumwan Bangkok 10330 Thailand Metallurgy and Materials Science Research Institute Chulalongkorn University Soi Chula 12 Phayathai Road Pathumwan Bangkok 10330 Thailand Electrochemistry and Optical Spectroscopy Research Unit Department of Chemistry Faculty of Science Chulalongkorn University Phayathai Road Pathumwan Bangkok 10330 Thailand Nanotec-CU Center of Excellent on Food and Agriculture Chulalongkorn University Phayathai Road Patumwan Bangkok 10330 Thailand

Zinc Oxide (ZnO) nanorods were synthesized by using zinc acetate as a precursor via thermal decomposition method and then used to prepare [email protected] nanocomposite by facile room-temperature approach using a colloidal coagulation effect. The as prepared [email protected] nanocomposite was applied to modify the working electrode surface in an electrochemical sensor of Cd 2+ and Pb 2+ for the first time. The morphologies of ZnO and [email protected] nanocomposite were characterized by transmission electron microscopy, that exhibits the nanorod shape with an average diameter of 89 ± 18 nm, uniformly dispersed and intercalated between each G layer. Furthermore, the important factors affecting on the sensor sensitivity, including [email protected] ratio, [email protected] concentration, Bi 3+ concentration and electrochemical parameters were systematically investigated using square-wave anodic stripping voltammetry. Under the optimal conditions, a linear range was found to be 10–200 μg L −1 and the detection limits were 0.6 and 0.8 μg L -1 for Cd 2+ and Pb 2+ , respectively. Eventually, it was successfully used for the simultaneous detection of Cd 2+ and Pb 2+ in real wastewater samples and the results corresponded well with a standard inductively coupled plasma-optical emission spectroscopy.

关键词： Zinc oxide nanorod Graphene Nanocomposite Electrochemical sensor Cadmium Lead

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Cell surface display:its application to biotechnology

Cell surface display:its application to biotechnology

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The 14th China-Japan-Korea Joint Symposium on Enzyme Engineering

作者： Chan Woo Park Joseph P.Park Haeshin Lee Seung Hwan Lee Interdisciplinary Program of Bioenergy and Biomaterials Graduate School Chonnam National University Graduate School of Nanoscience and Technology Korea Advanced Institute of Science and Technology

Cell surface display is a technique to express peptides or proteins on the surface of bacteria,fungi,or even mammalian cells by fusing them to various surface anchoring *** can be used a non-conventional method for immobilization of enzyme,which make this technology apply to the many *** now,several applications such as live vaccine,adsorbents,biosensors and whole cell

关键词： Cell surface display:its application to biotechnology

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Cell surface display of mussel inspired catecholamine-the preparation of sticky Escherichia coli

Cell surface display of mussel inspired catecholamine-the pr...

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The 14th China-Japan-Korea Joint Symposium on Enzyme Engineering

Cell surface display is a technique to display peptides or proteins on the surface of gram-negative and gram-positive bacteria,fungi,or even mammalian cells by appropriately fusing them to surface anchoring *** can be used a non-conventional method for immobilization of ***,we present a sticky *** which displayed catecholamine,a mussel-inspired adhesive moiety with catechol and amine functional group,was displayed on the cell surface using outer membrane protein

关键词： Cell surface display of mussel inspired catecholamine-the preparation of sticky Escherichia coli

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LifeTime and improving European healthcare through cell-based interceptive medicine (vol 587, pg 377, 2020)

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NATURE 2021年第7852期592卷 E8-E8页

作者： Rajewsky, Nikolaus Almouzni, Genevieve Gorski, Stanislaw A. Aerts, Stein Amit, Ido Bertero, Michela G. Bock, Christoph Bredenoord, Annelien L. Cavalli, Giacomo Chiocca, Susanna Clevers, Hans De Strooper, Bart Eggert, Angelika Ellenberg, Jan Fernandez, Xose M. Figlerowicz, Marek Gasser, Susan M. Hubner, Norbert Kjems, Jorgen Knoblich, Jurgen A. Krabbe, Grietje Lichter, Peter Linnarsson, Sten Marine, Jean-Christophe Marioni, John C. Marti-Renom, Marc A. Netea, Mihai G. Nickel, Dorthe Nollmann, Marcelo Novak, Halina R. Parkinson, Helen Piccolo, Stefano Pinheiro, Ines Pombo, Ana Popp, Christian Reik, Wolf Roman-Roman, Sergio Rosenstiel, Philip Schultze, Joachim L. Stegle, Oliver Tanay, Amos Testa, Giuseppe Thanos, Dimitris Theis, Fabian J. Torres-Padilla, Maria-Elena Valencia, Alfonso Vallot, Celine van Oudenaarden, Alexander Vidal, Marie Voet, Thierry Berlin Institute for Medical Systems Biology Max Delbrück Center for Molecular Medicine in the Helmholtz Association Berlin Germany Charité-Universitätsmedizin Berlin Germany Berlin Institute of Health (BIH) Berlin Germany German Center for Cardiovascular Research (DZHK) Partner Site Berlin Berlin Germany Institute for Biology Humboldt University of Berlin Berlin Germany Cardiovascular and Metabolic Sciences Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC) Berlin Germany Department of Pediatric Oncology/Hematology Charité-Universitätsmedizin Berlin Berlin Germany Institut Curie CNRS PSL Research University Sorbonne Université Nuclear Dynamics Unit Equipe Labellisée Ligue contre le cancer Paris France VIB Center for Brain and Disease Research Leuven Belgium Department of Human Genetics KU Leuven Leuven Belgium Department of Neurosciences KU Leuven Leuven Belgium UK Dementia Research Institute at UCL University College London London UK Wellcome Sanger Institute Wellcome Genome Campus Cambridge UK Department of Immunology Weizmann Institute of Science Rehovot Israel Centre for Genomic Regulation (CRG) Barcelona Institute of Science and Technology Barcelona Spain CNAG-CRG Centre for Genomic Regulation Barcelona Institute of Science and Technology Barcelona Spain Universitat Pompeu Fabra Barcelona Spain ICREA Barcelona Spain CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Vienna Austria Department of Laboratory Medicine Medical University of Vienna Vienna Austria Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases Vienna Austria Department of Medical Humanities Julius Center for Health Sciences and Primary Care University Medical Center Utrecht Utrecht The Netherlands Institute of Human Genetics UMR 9002 CNRS and University of Montpellier Montpellier France Department of Experimental Oncology IEO European Institute of Oncology IRCCS Milan Italy Department of Oncology and Hemato

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Influence of the electrodeposited co-fe alloys’ characteristics on their magnetic properties 2014

Influence of the electrodeposited co-fe alloys’ characteris...

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2014 International Conference on Advanced Materials, Structures and Mechanical Engineering, ICAMSME 2014

作者： Chotibhawaris, Thanakrit Luangvaranunt, Tachai Jantaratana, Pongsakorn Boonyongmaneerat, Yuttanant Nanoscience and Technology Interdisciplinary Program Graduate School Chulalongkorn University Phyathai Rd Pathumwan Bangkok Thailand Metallurgical Engineering Department Chulalongkorn University Phyathai Rd Pathumwan Bangkok Thailand Department of Physics Kasetsart University Ngam Wong Wan Rd Chatuchak Bangkok Thailand Chulalongkorn University Phyathai Rd Pathumwan Bangkok Thailand

ISBN: (纸本)9783038352549

Nanocrystalline CoFe films were fabricated by electrodeposition process for an investigation of the relationship between the alloys’ characteristics and their magnetic properties. The study shows that coating thickness promotes softer magnetic properties of the films, and induces changes of film roughness, preferred orientation and domain pattern. The preferred orientation of the ~1 μm films (thin films) is (110) plane, whereas that of the ~3 μm films (thick films) are (110) and (200) planes. The magnetic domain of the thin films exhibit a stripe-liked pattern, whereas a bubble-liked pattern appears in the thick films. Iron content significantly affects the magnetic properties of the thick films. In this study, the 57.3wt.%Fe thick film has the highest saturation magnetization, and the 80.0wt.%Fe thick film shows the lowest coercivity. © (2014) Trans Tech Publications, Switzerland.

关键词： Electrodeposition

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Influence of the Electrodeposited Co-Fe Alloys' Characteristics on their Magnetic Properties

Influence of the Electrodeposited Co-Fe Alloys' Characterist...

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The 2014 International Conference on Advanced Materials,Structures and Mechanical Engineering(ICAMSME 2014)

作者： Thanakrit Chotibhawaris Tachai Luangvaranunt Pongsakorn Jantaratana Yuttanant Boonyongmaneerat Nanoscience and Technology Interdisciplinary Program Graduate School Chulalongkorn University Metallurgical Engineering Department Faculty of Engineering Chulalongkorn University Department of Physics Faculty of Science Kasetsart University Metallurgy and Materials Science Research Institute (MMRI) Chulalongkorn University

Nanocrystalline CoFe films were fabricated by electrodeposition process for an investigation of the relationship between the alloys' characteristics and their magnetic properties. The study shows that coating thickness promotes softer magnetic properties of the films, and induces changes of film roughness, preferred orientation and domain pattern. The preferred orientation of the μm films（thin films） is（110） plane, whereas that of the μm films（thick films） are（110） and（200） planes. The magnetic domain of the thin films exhibit a stripe-liked pattern, whereas a bubble-liked pattern appears in the thick films. Iron content significantly affects the magnetic properties of the thick films. In this study, the 57.3wt.%Fe thick film has the highest saturation magnetization, and the 80.0wt.%Fe thick film shows the lowest coercivity.

关键词： Electrodeposition plating CoFe film magnetic properties

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Influence of structural parameters on electrical characteristics of schottky tunneling field-effect transistor and its scalability

Influence of structural parameters on electrical characteris...

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作者： Wu, Yan Dou, Chunmeng Wei, Feng Kakushima, Kuniyuki Ohmori, Kenji Ahmet, Parhat Watanabe, Takanobu Tsutsui, Kazuo Nishiyama, Akira Sugii, Nobuyuki Natori, Kenji Yamada, Keisaku Kataoka, Yoshinori Hattori, Takeo Iwai, Hiroshi Frontier Research Center Tokyo Institute of Technology Yokohama 226-8502 Japan Interdisciplinary Graduate School of Science and Engineering Tokyo Institute of Technology Yokohama 226-8502 Japan Honors Graduate Program for Nanotech/Sciences University of Tsukuba Tsukuba Ibaraki 305-8573 Japan Graduate School of Pure and Applied Sciences University of Tsukuba Tsukuba Ibaraki 305-8573 Japan Institute for Nanoscience and Nanotechnology Waseda University Shinjuku Tokyo 169-8555 Japan

The influence of structural parameters, including the Schottky barrier height for electron (Bn) and channel doping (Na), on the electrical characteristics of a scaled Schottky barrier tunneling FET (SBTFET) have been clarified by numerical device simulation. The thermionic emission current (ITH) as well as the tunneling current (ITN) have been considered as the main electron injections at the source edge. Simulation results have revealed that the main conduction is ITN in the region near and above the threshold voltage (Vth). As tunneling probability is determined by Bn and the width of the triangular potential barrier at the source edge, a lower Bn with higher Na results in a better subthreshold swing (SS) with high on-state drive current (ION) at a gate length (Lg) of 50 nm. With Lg scaling down to 10 nm, however, a lower Bn has shown an increased offstate leakage current (IOFF) due to the short-channel effect (SCE), while a larger Bn can suppress the IOFF at the cost of ION. Therefore, considering SS with ION and IOFF ratio, it can be concluded that an optimum Bn exists for short-channel devices. The SBTFET showed good subthreshold performance and higher ION=IOFF than the conventional silicon-on-insulator (SOI) MOSFET in 10nm region with the Schottky barrier height optimization. © 2013 The Japan Society of Applied Physics.

关键词： Threshold voltage

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Single-molecule fluorescence study on the phase shift and phase locking of RecA nucleoprotein filament

Single-molecule fluorescence study on the phase shift and ph...

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第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Sung Hyun Kim TakKyoon Ahn Chirlmin Joo Doseok Kim Interdisciplinary Program of Integrated Biotechnology Sogang UniversitySeoulRepublic of Korea Department of Physics Sogang UniversitySeoulRepublic of Korea Kavli Institute of NanoScience and Department of BioNanoScience Delft University of TechnologyDelftThe Netherlands

RecA protein lies at the core of DNA repair *** forms a helical structured filament on as single-strand DNA(ssDNA) appears as an intermediate of homologous *** the recent RecA crystal structure with embedded ssDNA,a RecA monomer binds to a group of three nucleotides and generates a gap in ***,there are three possible phases in the binding of RecA according to the relative position

关键词： RecA single molecule FRET DNA repair

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Linear and nonlinear plasmonic effects modulated by a metalinsulator transition

Linear and nonlinear plasmonic effects modulated by a metali...

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IEEE/LEOS Winter Topical Meeting Series

作者： R. F. Haglund E. U. Donev L. C. Feldman D. W. Ferrara R. Lopez J. Y. Suh K.A. Tetz Department of Physics and Astronomy Interdisciplinary Graduate Program in Materials Science and Vanderbilt Institute for Nanoscale Science and Engineering Vanderbilt University Nashville TN USA Department of Physics and Astronomy and Department of Materials Science and Engineering Rutgers University Piscataway NJ USA Department of Physics and Astronomy and Institute for Advanced Materials Nanoscience and Technology North Carolina State University Chapel Hill NC USA

Localized and propagating plasmonic effects in noble-metal nanostructures are receiving worldwide attention as potential enabling principles for optical and electro-optic devices. A critical need in plasmonic device design is a general technique for active modulation of the plasmon response. In this paper, we describe the use of a reversible, solid-solid phase transition in VO 2 to modulate plasmonic response. Case studies are drawn from experiments on nano structured hole and particle arrays in which VO 2 acts as a modulator by altering the local dielectric constant.

关键词： Plasmons Optical devices Optical propagation Nanostructures Nonlinear optical devices Nonlinear optics Optical modulation Electrooptic devices Phase modulation Dielectric constant

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