检索结果-内蒙古大学图书馆

GMNN mutations cause female infertility characterized by preimplantation embryo arrest through regulating DNA re-replication

引用

Science China Life Sciences 2025年

作者： Honghui Zhang Wei Su Xiaohong Jiang Yang Wang Bohan Yang Xian Wan Cheng Li Shuai Zhao Changlong Zhang Shigang Zhao Yuehong Bian Mei Li Keliang Wu Fei Gong Ge Lin Wei Zheng Han Zhao Zi-Jiang Chen Clinical Research Center for Reproduction and Genetics in Hunan Province Reproductive and Genetic Hospital of CITIC-XIANGYA NHC Key Laboratory of Human Stem Cell and Reproductive Engineering School of Basic Medical Sciences Central South University Center for Medical Genetics School of Life Sciences Central South University Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Department of Reproductive Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine State Key Laboratory of Reproductive Medicine and Offspring Health Center for Reproductive Medicine Institute of Women Children and Reproductive Health Shandong University National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong University Key Laboratory of Reproductive Endocrinology (Shandong University) Ministry of Education Shandong University Shandong Technology Innovation Center for Reproductive Health Shandong Provincial Clinical Research Center for Reproductive Health Shandong Key Laboratory of Reproductive Research and Birth Defect Prevention Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical Sciences (No.2021RU001) The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Municipal Hospital Gusu School Nanjing Medical University The Second Affiliated Hospital Hengyang Medical School University of South China

Preimplantation embryo arrest is a common cause of female infertility and recurrent failure of assisted reproductive technology. However, its genetic basis is largely unrevealed. Geminin, encoded by the GMNN gene, plays an important role in preventing DNA re-replication by inhibiting CDT1. Here, using whole-exome sequencing and Sanger sequencing, we identified three rare missense mutations of GMNN in females with preimplantation arrest, following a dominant inheritance pattern. The RNA sequencing data from both the mouse zygotes and the patient's one-cell embryo demonstrated the altered cell cycle processes. We then found that these mutations decreased the binding with CDT1 and resulted in activation of CHK1 and DNA damage, resulting in cell cycle disturbance. Our findings uncover a mechanistic understanding of the pathogenesis of human preimplantation embryo arrest, which acts by impairing the correct cell cycle and DNA rereplication procedure, and provides a new molecular target for the diagnosis and treatment of infertile patients.

关键词：

来源：评论

学校读者我要写书评

暂无评论

X-scPAE: An explainable deep learning model for embryonic lineage allocation prediction based on single-cell transcriptomics revealing key genes in embryonic cell development

引用

Computers in Biology and Medicine 2025年 188卷 109787-109787页

作者： Liao, Kai Yan, Bowei Ding, Ziyin Huang, Jian Fan, Xiaodan Wu, Shanshan Chen, Changshui Li, Haibo The Central Laboratory of Birth Defects Prevention and Control The Affiliated Women and Children's Hospital of Ningbo University Ningbo315021 China Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases The Affiliated Women and Children's Hospital of Ningbo University Ningbo315021 China Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention The Affiliated Women and Children's Hospital of Ningbo University Ningbo315021 China MOE Engineering Research Center of Gene Technology School of Life Sciences Fudan University Shanghai200433 China Institutes of Biomedical Sciences Fudan University Shanghai200433 China Center for Reproductive Medicine The Affiliated Women and Children's Hospital of Ningbo University Ningbo315021 China College of Computer Science Chongqing University Chongqing400044 China

In single-cell transcriptomics research, accurately predicting cell lineage allocation and identifying differences between lineages are crucial for understanding cell differentiation processes and reducing early pregnancy miscarriages in humans. This paper introduces an explainable PCA-based deep learning attention autoencoder model, X-scPAE (eXplained Single Cell PCA - Attention Auto Encoder), which is built on the Counterfactual Gradient Attribution (CGA) algorithm. The model is designed to predict lineage allocation in human and mouse single-cell transcriptomic data, while identifying and interpreting gene expression differences across lineages to extract key genes. It first reduces dimensionality using Principal Component Analysis (PCA) and ranks the importance of principal components. An autoencoder is then employed for feature extraction, integrating an attention mechanism to capture interactions between features. Finally, the Counterfactual Gradient Attribution algorithm calculates the importance of each feature. The model achieved an accuracy of 0.945 on the test set and 0.977 on the validation set, with other metrics such as F1-score, Precision, and Recall all reaching 0.94. It significantly outperformed both baseline algorithms (XGBoost, SVM, RF, and LR) and advanced approaches like F-Score-SVM, CV2-LR, scChrBin, and TripletCell. Notably, the explainability analysis uncovered key lineage predictor genes for both humans and mice and identified crucial genes distinguishing between developmental stages and lineages. A logistic regression model built using the extracted key genes still achieved an AUROC of 0.92, surpassing the performance of other feature extraction methods, including F-Score, CV2, PCA, random feature selection, and the interpretability method Shapley. Lastly, ablation studies demonstrated the effectiveness of each model component. © 2025 The Authors

关键词： Mammals

来源：评论

学校读者我要写书评

暂无评论

NOP2-mediated m5C Modification of c-Myc in an EIF3A-Dependent Manner to Reprogram Glucose Metabolism and Promote Hepatocellular Carcinoma Progression

引用

研究（英文） 2023年第6期2023卷 400-416页

作者： Hao Zhang Xiangyu Zhai Yanfeng Liu Zhijia Xia Tong Xia Gang Du Huaxin Zhou Dorothee Franziska Strohmer Alexandr V.Bazhin Ziqiang Li Xianqiang Wang Bin Jin Deliang Guo Department of Hepatobiliary and Pancreatic Surgery Zhongnan Hospital of Wuhan UniversityWuhanChina Department of Hepatobiliary Surgery The Second Hospital of Shandong UniversityJinanChina Organ Transplant Department Qilu Hospital of Shandong UniversityJinanChina Department of Hepatobiliary Surgery The Second Hospital of Shandong UniversityJinanChina Organ Transplant Department Qilu Hospital of Shandong UniversityJinanChina Department of Hepatobiliary Surgery Qilu Hospital of Shandong UniversityJinanChina Department of General Visceraland Transplant SurgeryLudwig-Maximilians-University MunichMunichGermany Organ Transplant Department Qilu Hospital of Shandong UniversityJinanChina Department of Hepatobiliary Surgery The Second Hospital of Shandong UniversityJinanChina Department of Hepatobiliary and Pancreatic Surgery Zhongnan Hospital of Wuhan UniversityWuhanChina Department of Pediatrics Surgery The Seventh Medical Center of PLA General HospitalNational Engineering Laboratory for Birth Defects Prevention and Control of Key TechnologyBeijing Key Laboratory of Pediatric Organ FailureBeijingChina

Mitochondrial dysfunction and glycolysis activation are improtant hallmarks of hepatocellular carcinoma(HCC).NOP2 is an S-adenosyl-L-methionine-dependent methyltransferase that regulates the cell cycle and proliferation *** this study,found that NOP2 contributes to HCC progression by promoting aerobic *** results revealed that NOP2 was highly expressed in HCC and that it was associated with unfavorable ***2 knockout in combination with sorafenib enhanced sorafenib sensitivity,which,in turn,led to marked tumor growth ***,we identified that NOP2 regulates the c-Myc expression in an m5C-modification manner to promote ***,our results revealed that m5C methylation induced c-Myc mRNA degradation in an eukaryotic translation initiation factor 3 subunit A(EIF3A)-dependent *** addition,NOP2 was found to increase the expression of the glycolytic genes LDH A,TPI1,PKM2,and ***,MYC associated zinc finger protein(MAZ)was identified as the major transcription factor that directly controlled the expression of NOP2 in ***,in a patient-derived tumor xenograft(PDX)model,adenovirus-mediated knockout of NOP2 maximized the antitumor effect and prolonged the survival of PDX-bearing *** cumulative findings revealed the novel signaling pathway MAZ/NOP2/c-Myc in HCC and uncovered the important roles of NOP2 and m5C modifications in metabolic ***,targeting the MAZ/NOP2/c-Myc signaling pathway is suggested to be a potential therapeutic strategy for the treatment of HCC.

关键词： Mammals

来源：评论

学校读者我要写书评

暂无评论

Oxygen Atom‑Concentrating Short Fibrous Sponge Regulates Cellular Respiration for Wound Healing

引用

Advanced Fiber Materials 2023年第5期5卷 1773-1787页

作者： Xiaohan Fu Juan Wang Dejian Qian Linhe Xi Liang Chen Yawei Du Wenguo Cui Yan Wang Department of Plastic Surgery Key Laboratory of Birth Regulation and Control Technology of National Health Commission of ChinaShandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao UniversityJinan 250014People’s Republic of China Department of Orthopedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopedicsRuijin HospitalShanghai Jiao Tong University School of Medicine197 Ruijin 2nd RoadShanghai 200025People’s Republic of China Department of Orthopedic Surgery The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan HospitalJinan 250014People’s Republic of China Department of Plastic Surgery The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan HospitalJinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound RepairJinan 250014People’s Republic of China

Cellular respiration can provide energy for wound ***,some of retarded healing processes in local hyperglycemic environment suffer from a decrease in cellular adaptation to oxygen,thus reducing in situ oxidative ***,a three-dimensional(3D)extracellular matrix(ECM)bionic short fibrous sponge was prepared for chronic diabetic wound healing and effectively regulated cellular respiration by enhancing cellular adaptation to oxygen and remolding the local tissue *** 3D bionic sponge scaffold exhibited good cell adhesion,biocompatibility,bioactivity,and,most importantly,aggregated oxygen atoms on the graphene oxide(GO)*** an in vitro assay,the oxygen atom-concentrating short fibrous sponge activated monocyte chemoattractant protein-1(MCP-1),induced the expression of vascular endothelial growth factor(VEGF),and effectively promoted angiogenesis in a hyperglycemic *** sponge was also applied to diabetic wounds in vivo to verify its roles in the promotion of angiogenesis and collagen *** experiments confirmed the synergistic effect of GO with adipose-derived stem cells(ADSCs),which could further promote diabetic wound ***,oxygen atom-concentrating short fibrous sponges that regulate cellular respiration provide a new idea for the repair of poorly healing wounds by improving oxidative metabolism and have importantclinical significance.

关键词： Cellular respiration Graphene oxide MCP-1 Short fibrous sponge Wound healing

来源：评论

学校读者我要写书评

暂无评论

Application of next generation sequencing in the screening of monogenic diseases in China,2021:a consensus among Chinese newborn screening experts

引用

World Journal of Pediatrics 2022年第4期18卷 235-242页

作者： Fan Tong Jian Wang Rui Xiao Bing-Bing Wu Chao-Chun Zou Ding-Wen Wu Hua Wang Hui Zou Lian-Shu Han Lin Yang Lin Zou Ming-Yan Hei Ru-Lai Yang Tian-Ming Yuan Wei Wen Xin-Wen Huang Xue-Fan Gu Yan-Ling Yang Yong-Lan Huang Yong-Jun Zhang Yong-Guo Yu Zheng-Feng Xu Wen-Hao Zhou Zheng-Yan Zhao Children’s Hospital Zhejiang University School of MedicineNational Clinical Research Center for Child Health3333 Binsheng RoadBinjiang DistrictHangzhou 310052China Shanghai Children’s Medical Center Shanghai Jiaotong University School of MedicineShanghaiChina National Engineering Laboratory for Key Technology of Birth Defect Control and Prevention Screening and Diagnostic R and D CenterHangzhouChina Center for Molecular Medicine Children’s Hospital of Fudan University399 Wan Yuan RoadMin Xing DistrictShanghai 200000China National Health Commission Key Laboratory for Birth Defect Research and Prevention Maternal and Child Health Hospital of Hunan ProvinceChangshaChina Jinan Maternity and Child Care Hospital Affiliated To Shandong First Medical University JinanChina Xinhua Hospital Shanghai Institute for Pediatric ResearchShanghai Jiao Tong University School of MedicineShanghaiChina Clinical Genetic Center Children’s Hospital of Fudan UniversityShanghaiChina Children’s Hospital Shanghai Jiaotong UniversityShanghaiChina Neonatal Center Beijing Children’s HospitalCapital Medical UniversityBeijingChina Affiliated Shenzhen Maternity and Child Healthcare Hospital Southern Medical UniversityShenzhenChina Xinhua Hospital School of MedicineShanghai Jiaotong UniversityShanghaiChina Peking University First Hospital BeijingChina Guangzhou Women and Children’s Medical Center GuangzhouChina Center of Genetic Medicine Women’s Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingChina

Newborn screening(NBS)refers to a maternal and newborn healthcare technology,in which special examinations of congenital and genetic diseases that could seriously impact the health of newborns,are implemented during the neonatal period to provide early diagnosis and treatment[1].With a history of more than 60 years,NBS has advanced greatly due to technological progress resulting in significant improvement in the number of diseases covered by NBS and in screening efficiency[2-7].

关键词： diseases diagnosis congenital

来源：评论

学校读者我要写书评

暂无评论

Silk Fibroin Protein and Graphene Synergistically Boosting the Reactive Oxygen Species Generation of Pu/Agi Photocatalytic Membrane for Tetracycline Sustained Removal

SSRN

引用

SSRN 2024年

作者： Zhao, Xiufei Liu, Xiaowen Chen, Wuhui Zhang, Yusheng Zhou, Hu Zhang, Lei Key Laboratory of Theoretical Organic Chemistry and Functional Molecule MoE College of Chemistry and Chemical Engineering Hunan University of Science and Technology Xiangtan411201 China School of Resource and Environment and Safety Engineering Hunan University of Science and Technology Xiangtan411201 China Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control Changsha Hospital for Maternal and Child Health Care Hunan Normal University Changsha410007 China Scientific Research Department Jiashangtong Interchange Energy Group Co. Ltd Changsha410007 China

Solar-light driven organic pollutants degradation by the recyclable photocatalytic membrane materials emerges as a promising technology for sewage purification. However, low generation of reactive oxygen species severely constraints their photocatalytic activity. Herein, we introduce a novel hydrophilic PU/SF/GO/AgI composite photocatalytic membrane fabricated via adding silk fibroin (SF) and graphene oxide (GO) for TC removal. By visible light irradiation, the PU/SF/GO/AgI membrane with 66.7 wt% SF and 0.05 wt% GO degrades TC with 7-fold increase in comparison with the PU/AgI membrane. The enhance photocatalytic activity is primarily attributed to its efficient generation of reactive oxygen species facilitated by the improved hydrophilicity and boosted charge separation. FTIR and electrochemical results demonstrate that the SF and GO with rich surface oxygen-containing groups contribute to the formation of Ag-O bonds for accelerating charge migration and separation. Significantly, the improved hydrophilicity of PU/SF/GO membrane can not only provide rich binging sites for AgI loading, but also benefit to attract small molecules for facilitating to reactive oxygen species generation. As a result, •O2-, •OH and H2O2 concentrations produced in PU/SF/GO/AgI membrane system reaches up to 53.20 μmol g-1 h-1, 7.89 μmol g-1 h-1 and 16.52 μmol, respectively, 6.7, 15.4 and 5.1-times higher than PU/AgI membrane system. Meanwhile, under LED irradiation of 12 h, TC degradation efficiency by the dynamic membrane reactor equipped with PU/SF/GO/AgI can reach up to 53% , exceeding the pure PU/AgI. This work offers an effective strategy for constructing sustained solar-light driven photocatalytic membrane system. © 2024, The Authors. All rights reserved.

关键词： Membranes

来源：评论

学校读者我要写书评

暂无评论

Population-level analyses identify host and environmental variables influencing the vaginal microbiome

引用

Signal Transduction and Targeted Therapy 2025年第3期10卷 1677-1691页

作者： Lang Qin Tianyong Sun Xiao Li Shigang Zhao Zheng Liu Changlong Zhang Congcong Jin Yanqi Xu Xuan Gao Yongzhi Cao Jiaojiao Wang Ting Han Lei Yan Jialun Song Fangfang Zhang Feifei Liu Yousheng Zhang Yuzhen Huang Yuping Song Yanjun Liu Jing Zhang Xiuqing Zhang Zhina Yao Honglei Chen Zhenzhen Zhang Shengrui Zhao Yuhan Feng Ya-nan Zhang Qian Yu Fang Cao Lijuan Zhao Lei Xie Ling Geng Qiang Feng Han Zhao Zi-Jiang Chen State Key Laboratory of Reproductive Medicine and Offspring Health Center for Reproductive MedicineInstitute of WomenChildren and Reproductive HealthShandong UniversityJinanShandong 250012China Key Laboratory of Reproductive Endocrinology(Shandong University) Ministry of EducationJinanShandong 250012China Shandong Key Laboratory of Reproductive Research and Birth Defect Prevention JinanShandong 250012China Shandong Technology Innovation Center for Reproductive Health JinanShandong 250012China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinanShandong 250012China Department of Human Microbiome School and Hospital of StomatologyCheeloo College of MedicineShandong UniversityJinan 250012 ShandongChina Shandong Provincial Key Laboratory of Oral Tissue Regeneration Jinan 250012 ShandongChina Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration Jinan 250012 ShandongChina Reproductive Medicine Center of the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 ZhejiangChina Linyi People’s Hospital Linyi 276000 ShandongChina Department of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021 ShandongChina State Key Laboratory of Microbial Technology Shandong UniversityQingdaoChina Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200135China Department of Reproductive Medicine Ren Ji HospitalShanghai Jiao Tong University School of MedicineShanghai 200135China

The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely ***,we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese *** 16S rRNA sequencing,we identified four core vaginal microbiota with a prevalence of over 90%and a total median abundance of 98.8%.Twenty-four variables,including physiology,lifestyle behaviors,gynecologic history,social and environmental information,were found associated with the microbiome composition,of which bacterial vaginosis(BV)showed the largest effect *** was among the strongest explanatory variables and the vaginal microbiome dynamically succeeded with increasing age,especially with a composition turning point at the age of *** mediation analyses indicated that the effects of age on the microbiome could be mediated by variables such as parity number and *** further classified the vaginal microbiomes of the population into 13“Vagitypes”.Women with Lactobacillus iners-and Lactobacillus jensenii-dominated Vagitypes had significantly higher live birth rate than those with Vagitype dominated by Fannyhessea vaginae(53.40%,59.09%vs 21.43%;OR[95%CI]:3.62[1.12-14.87],5.39[1.27-27.36];P=0.031,P=0.021).This study provides a comprehensive overview of the associations between identified variables and the vaginal microbiome,representing an important step toward understanding of environment-microbe-host interactions.

关键词： host environmental variables s rrna sequencingwe core vaginal microbiota environmental variables vaginal microbiome host variables reproductive health bacterial vaginosis

来源：评论

学校读者我要写书评

暂无评论

Target Analysis and Suspect Screening of Per- and Poly-Fluoroalkyl Substances in Paired Samples of Maternal Serum, Umbilical Cord Serum, and Placenta Near Fluorochemical Plants in Fuxin, China

SSRN

引用

SSRN 2022年

作者： Bao, Jia Shao, Li-Xin Liu, Yang Cui, Shi-Wei Wang, Xin Lu, Gui-Lin Wang, Xue Jin, Yi-He School of Environmental and Chemical Engineering Shenyang University of Technology Shenyang110870 China The National Institute for Occupational Health and Poison Control Chinese Center for Disease Control and Prevention Beijing100050 China Key Laboratory of Industrial Ecology and Environmental Engineering MOE School of Environmental Science and Technology Dalian University of Technology Dalian116024 China

The levels of legacy per- and poly-fluoroalkyl substances (PFASs) have been progressively growing in the environmental matrices and blood of residents living around the fluorochemical industrial park (FIP) in Fuxin of China over the past decade. Although some recent studies have reported occurrence of novel PFAS alternatives in biotic and abiotic matrices near fluorochemical facilities worldwide, little is known about novel PFAS congeners in maternal sera, umbilical cord sera, and placentas from the female residents close to the FIP and their possibly related health risks. In the present study, 50 paired samples of maternal and cord serum as well as placenta were derived from Fuxin pregnant women at delivery, and 21 target analytes of legacy PFASs in all the samples were analyzed via high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS), revealing that PFBS, PFBA, and PFOA were the dominant PFAS contaminants observed in the whole samples with an overall increasing trend recently. Based upon the suspect screening through high-resolution mass spectrometry (HRMS), 49 novel PFASs assigned to 11 classes were further identified in the Fuxin samples, of which, 20 novel congeners in 4 classes were reported in human blood and placentas for the first time. Moreover, the coefficients for mother-placenta transfer (Rm/p), placenta-newborn transfer (Rp/n), and mother-newborn transfer (Rm/n) of legacy PFASs could be calculated with median values of 1.7, 1.1, and 2.0, respectively, and Rm/p, Rp/n, and Rm/n for each novel PFAS identified were also estimated with the median values of 0.9, 1.2, and 0.8 individually. Subsequently, possible biotransformation pathways of legacy PFSAs and PFCAs during placental transfer could also be proposed. In addition, significant correlations were determined among the neonate birth outcomes and serum concentrations of thyroid hormone, sex hormone, and glucocorticoid, together with the levels of certain legacy and novel PFASs in

关键词： Screening

来源：评论

学校读者我要写书评

暂无评论

Fault Diagnosis of Roadheader Cutting Head Based on Improved RCMDE and Optimised Random Forests

引用

Science technology and engineering 2025年第9期25卷 3629-3636页

作者： Ma, Tian-Bing Yang, Ting Li, Chang-Peng Du, Fei Shi, Rui Yu, Ping-Ping State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Anhui University of Science and Technology Huainan232001 China Institute of Energy Hefei Comprehensive National Science Center [Anhui Energy Laboratory Hefei230051 China School of Mechanical Engineering Anhui University of Science and Technology Huainan232001 China

To address the challenges of extracting and identifying fault features from roadheader cutting vibration signal, a new fault diagnosis method of roadheader cutting head based on the refine composite multi-scale fuzzy dispersion entropy(RCMFDE) and hippo optimized random forest(HORF) was proposed. Firstly, RCMFDE was used to comprehensively characterize the fault feature information of the roadheader cutting head, and the fault feature data set was constructed. Secondly, the fault type was trained and tested by the HORF to realize the fault pattern recognition of the cutting head of the roadheader. Finally, the proposed method was applied to the experimental data analysis of the cutting head of the roadheader, and compared with the existing multi-scale fuzzy entropy and fine-complex multi-scale spread entropy fault feature extraction methods. The results of the trial indicate that RCMFDE performs better than the other two entropy approaches in discovering defect features, and hippo random forest outperforms extreme learning machine and support vector machine in error recognition. The fault diagnosis method can more correctly recognize the error type of the cutting head of the roadheader, and the rate of accuracy of the recognition obtained 100% . © 2025 Beijing Kexue Jishu yu Gongcheng Zazhishe. All rights reserved.

关键词： Feature Selection

来源：评论

学校读者我要写书评

暂无评论

CTCF organizes inter-A compartment interactions through RYBP-dependent phase separation

引用

细胞研究（英文版） 2022年第8期32卷 744-760页

作者： Chao Wei Lumeng Jia Xiaona Huang Jin Tan Mulan Wang Jing Niu Yingping Hou Jun Sun Pengguihang Zeng Jia Wang Li Qing Lin Ma Xinyi Liu Xiuxiao Tang Fenjie Li Shaoshuai Jiang Jingxin Liu Tingting Li Lili Fan Yujie Sun Juntao Gao Cheng Li Junjun Ding RNA Biomedical Institute Sun Yat-Sen Memorial HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdongChina Advanced Medical Technology Center The First Affiliated HospitalZhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdongChina Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdongChina School of Life Sciences Peking UniversityBeijingChina School of Medicine Tsinghua UniversityBeijingChina Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking UniversityBeijingChina RNA Biomedical Institute Sun Yat-Sen Memorial HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdongChina Advanced Medical Technology Center The First Affiliated HospitalZhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdongChina Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdongChina Department of Cell Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdongChina RNA Biomedical Institute Sun Yat-Sen Memorial HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdongChina Advanced Medical Technology Center The First Affiliated HospitalZhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdongChina Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdongChina Department of Pediatric Surgery Guangdong Provincial Key Laboratory of Research in Structural Birth Defect DiseaseGuangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhouGuangdongChina State Key Laboratory of Proteomics National Ce

Chromatin is spatially organized into three-dimensional structures at different levels including A/B compartments,topologically associating domains and *** canonical CTCF-mediated loop extrusion model can explain the formation of ***,the organization mechanisms underlying long-range chromatin interactions such as interactions between A-A compartments are still poorly *** we show that different from the canonical loop extrusion model,RYBP-mediated phase separation of CTCF organizes inter-A compartment *** on this model,we designed and verified an induced CTCF phase separation system in embryonic stem cells(ESCs),which facilitated inter-A compartment interactions,improved self-renewal of ESCs and inhibited their differentiation toward neural progenitor *** findings support a novel and non-canonical role of CTCF in organizing long-range chromatin interactions via phase separation.

关键词： Chromatin structure Pluripotency

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：