Hypochlorous acid （HOCI） is one of highly reactive oxygen species （ROS）. It is involved in both immune defense against invading microbes and the progression of many diseases including cardiovascular disease and ne...
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Hypochlorous acid （HOCI） is one of highly reactive oxygen species （ROS）. It is involved in both immune defense against invading microbes and the progression of many diseases including cardiovascular disease and neurodegeneration disorders. It is generated from hydrogen peroxide （H2O2） and chloride ions in the presence of myeloperoxidase in activated neutrophils. To illustrate HOCI＇s biological functions, fluorescent probes, particularly those fluorescence emissions are in the near-infrared range, are highly needed for in vivo applications. Herein, we reported the design of a pinacol boronate caged near-infrared （NIR） fluorescent probe I derived from an Aft binding fluorophore NIAD-4 for fast and selective detection of HOC1/C10 over other ROS. Upon exposure to HOCI/CIO-, the pinacol boronate caging group of the probe 1 was quickly converted to electron-donating hydroxyl group, which increased intramolecular charge transfer OCT） in the excited state and resulted in the red-shift and intensity enhancement of fluorescence emission. The probe bears several unique features： （1） It could be used as either a ratiomatic or turn-on fluorescent probe; （2） Reaction of the caging group boronate with HOCI is very fast and finishes within seconds, which provides the selectivity over H2O2; （3） The NIAD-4 fluorophore provides additional selectivity for detection of HOCl over peroxynitrite. Moreover, the utility of the probe in imaging HOCllClO- was demonstrated in in vitro phantom imaging studies using mouse brain homogenate as biological relevant media.
The large thermal energy in launching because of high-current and high-speed affect the engineering application of electromagnetic rail launcher. In this paper, considering the inductance gradient and the resistance g...
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ISBN:
(纸本)9781728133997
The large thermal energy in launching because of high-current and high-speed affect the engineering application of electromagnetic rail launcher. In this paper, considering the inductance gradient and the resistance gradient, the circuit model of Electromagnetic Rail Launcher including PFN, rail, armature and cable is established, the motion model of armature position, speed and current are taken for interative analytical calculation by using Matlab. The data contrast with other literature and experimental shows that the model is correct and effective. The heating effect analysis of Electromagnetic Rail Launcher include Joule energy of rail resistance, cable and induction, friction energy between armature and rail, etc. Comparison with the kinetic energy of projectile, the thermal energy of rail including resistance and friction and Joule energy of cable take a large proportion in system energy. The paper provides a basis for the cooling design of Electromagnetic Rail Launcher.
With the development of electronic equipment integration technology, the air demand for cooling in cabin and electronic system is increasing continuously. The ram air outlet plays an important role in the airflow syst...
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With the development of electronic equipment integration technology, the air demand for cooling in cabin and electronic system is increasing continuously. The ram air outlet plays an important role in the airflow system. In this paper, the three-dimensional modeling of the door-type ram air outlet and the grille-type ram air outlet is carried out. Then, the flow characteristics and resistance characteristics of ram air outlet are analyzed based on structured grid technology. The results show that the flow rate of the door panel ram air outlet is greater than that of the grille-type ram air outlet in the same size, the discharge rate of the door panel outlet is 0.3kg/s more than that of the grille-type outlet under the design condition, and the skin surface temperature at the back of the door panel outlet is 13.5K lower than that of the grille outlet. Therefore, the door-type ram air outlet has better emission characteristics and flow characteristics, which is more suitable for the aircraft in this research. This study provides a technical reference for the design of the civil aircraft.
An optimization design of china supercritical water-cooled reactor (SCWR) with the rated electric power of 1000 MWe (CSR1000) conceptual core is proposed. Steady-state performance of the proposed core is then studied ...
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A low profile microstrip antenna with adjustable bandwidth around 3 GHz is proposed. The compact antenna consists of a square patch with a square aperture to tune the resonance center frequency. The input impedance ba...
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In the severe high-temperature environment caused by aerodynamic heating, the vibrational excitation, dissociation and ionization of gas may successively occur, which are known as real gas effects. Under the real gas ...
In the severe high-temperature environment caused by aerodynamic heating, the vibrational excitation, dissociation and ionization of gas may successively occur, which are known as real gas effects. Under the real gas effects, the thermodynamic properties of gas vary drastically and significantly influence the performances of the active thermal protection system of hypersonic vehicles, especially in the case with coolant outflow, for example transpiration cooling. This paper numerically investigates the transpiration cooling performance with the consideration of the interaction between coolant outflow and hypersonic flow under the real gas effects. The mathematical models and coupled numerical strategy are firstly validated by experimental data, then the influences of real gas effects on the transpiration cooling of a wedged leading edge (WLE) are studied under a flight Mach number range from 8 to 12 and a flight height of 40 km. The analysis and discussions of the numerical results reveal some important phenomena and demonstrate the need to consider real gas effects.
作者：
Ling-Ling WuWeihua DiLitao ShaoJie ZhouYu-Yu QiuMengrui ZhangZhenye LiYanqun ZhangPengcheng LuanJifan LiGongchang YuGuiqian FangCaicai MengBin ShiXuemei ZhaoQixin ChenXintian ShaoSchool of Pharmaceutical Sciences
National Key Laboratory of Advanced Drug Delivery System Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250062 PR China. Department of Traditional Chinese Medicine Orthopedics
Neck-Shoulder and Lumbocrural Pain Hospital of Shandong First Medical University Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250062 PR China. School of Life Sciences
Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250117 PR China. Department of Traditional Chinese Medicine Orthopedics
Neck-Shoulder and Lumbocrural Pain Hospital of Shandong First Medical University Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250062 PR China. Electronic address: bshi@***. School of Pharmaceutical Sciences
National Key Laboratory of Advanced Drug Delivery System Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250062 PR China. Electronic address: zhaoxm@***. School of Pharmaceutical Sciences
National Key Laboratory of Advanced Drug Delivery System Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250062 PR China Departments of Diagnostic Radiology
Surgery Chemical and Biomolecular Engineering and Biomedical Engineering Yong Loo Lin School of Medicine and College of Design and Engineering National University of Singapore Singapore 119074 Singapore. School of Life Sciences
Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250117 PR China Department of Traditional Chinese Medicine Orthopedics
Neck-Shoulder and Lumbocrural Pain Hospital of Shandong First Medical Univ
BACKGROUND:RECQL4 is a critical factor in DNA repair and serves as a significant biomarker for liver cancer. Inhibiting RECQL4 induces apoptosis in liver cancer cells. Given the role of mitochondria in regulating apop...
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BACKGROUND:RECQL4 is a critical factor in DNA repair and serves as a significant biomarker for liver cancer. Inhibiting RECQL4 induces apoptosis in liver cancer cells. Given the role of mitochondria in regulating apoptosis and the dual localization of RECQL4 in both the mitochondria and nucleus, targeting mitochondrial RECQL4 may induce mitochondrial dysfunction, thereby enhancing the therapeutic efficacy of liver cancer treatments.
PURPOSE:Unlike traditional drug structure optimization, which focuses solely on protein target interactions, our approach optimizes drug structure based on both organelle distribution and protein interactions.
METHODS:As a proof of concept, we selected Rhein (4,5-dihydroxyanthraquinone-2-carboxylic acid), an auto-fluorescent, lipophilic anthraquinone found in a variety of different medicinal herbs used in TCM that is known to bind to RECQL4, a protein that is distributed throughout the cytosol, mitochondria, and other subcellular locations, albeit unevenly. Rhein has the tendency to accumulate in different subcellular organelles, which reduces the overall efficacy of the drug. To overcome this limitation, we conjugated a triphenylphosphonium group Rhein to direct the drug to the mitochondria, resulting in Rh-Mito.
RESULTS:Our results demonstrated that this chemical modification retains the ability to intervene in the expression of RECQL4 protein while also improving the cellular uptake of Rh-Mito. Utilizing advanced imaging techniques, we show that Rh-Mito preferentially accumulated in the mitochondria, where it targeted RECQL4 to inhibit the repair of mtDNA, altered the morphology of mitochondrial cristae, and induced apoptosis. Notably, Rh-Mito displayed superior anti-tumor efficacy compared to unmodified Rhein.
CONCLUSION:Rh-Mito was designed to selectively target mitochondria, avoiding interactions with other organelles. This modification strengthens its binding to RECQL4, disrupts mitochondrial cristae, inhibits tumor cell migration
Because of the high temperature and high pressure characteristics of supercritical water-cooled reactor (SCWR), the thermal hydraulic performance of SCWR is greatly different from pressurized water reactor (PWR), whic...
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In mobile communication networks, the security of users' data depends on the guarantees provided by the authentication protocols. For the next generation of mobile network (i.e., 5G network), the 5G-EAP-TLS protoc...
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In mobile communication networks, the security of users' data depends on the guarantees provided by the authentication protocols. For the next generation of mobile network (i.e., 5G network), the 5G-EAP-TLS protocol has been proposed for this purpose. However, it is still unknown whether this protocol provides the claimed security guarantees or not. To fill this gap, we provide in this work the first formal model of 5G-EAP-TLS protocol and conduct a thorough analysis based on Scyther model checker. Secondly, we identify several design flaws in the protocol, which may jeopardize the security goals and result in severe security vulnerabilities when implemented in real systems. To our knowledge, this is the first study on the formal verification of 5G-EAP-TLS protocol.
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