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Reduction on OFF-responses of Electroretinogram in Monkeys with Long-term High Intraocular Pressure

Chinese Medical Journal 2017年第22期130卷 2713-2719页

作者： Ke-Gao Liu Xiao-Yan Peng Zheng Zhang Hua Sun Di-Ya Yang Ning-Li Wang Department of Ophthalmology Beijing Tongren Eye Center Beijing Ophthalmology and Visual Science Key Lab Beijing Tongren Hospital Capital Medical University Beijing 100730 China Beijing Tongren Eye Center Beijing Institute of Ophthalmology Beijing Tongren Hospital Capital Medical University Beijing 100730 China

Background： There are ON- and OFF-pathways in the normal vertebrate retina. Short- and long-flash electroretinogram （ERG） are suitable methods to observe the function of ON- and OFF-pathways in vivo, respectively. It is clear that high intraocular pressure （IOP） might cause dysfunction of cone-dominated photopic negative response （PhNR） in monkeys with high lOP in ON-pathway. However, whether cone-dominated OFF-responses are also affected is less known. The aim of this study was to observe photopic OFF-responses of ERG in monkeys with high lOP. Methods： Nine monkeys were involved in the experiment from January 2006 to December 2016. High IOP was induced in the right eye by laser coagulation of the mid-trabecular meshwork in five monkeys. Six years after the laser coagulation, both short- and long-flash of the photopic ERG were recorded. Stimulus light was red flashes superimposed on a blue background. Four normal monkeys were examined under the same ERG protocols as controls. Paired t-test was used to compare the difference of each ERG parameter between the lasered eye and the fellow eye. Analysis of variance （ANOVA） with Tukey adjustment was adopted to calculate the differences among the lasered eye, the fellow eye, and the eyes of normal monkeys. Results： The mean amplitude of a-wave （11.73 ± 2.05） and PhNR （8.67 ± 2.44） in lasered eyes was significantly lower than that of a-wave （21.47 ±3.15） and PhNR （22.05 ± 3.42） in fellow eyes （P = 0.03 and P 0.01, respectively） in response to short flash. The mean amplitude old-wave （ 1.60 ± 0.59） and/-wave （3.13± 0.64） was significantly reduced in the lasered eyes than that of d-wave （4.01 ± 0.56） and/-wave （8.79 ± 1.75） in the fellow eyes （P =0.02 and P = 0.02, respectively） in response to long flash. Conclusions： Reduced OFF-responses are recorded in monkeys with high IOP when dysfunction ofphotoreceptor is involved. The reduced OFF-responses to long-flash stimulus show evidence of anomalous retinal circuitry in glaucomat

关键词： Electroretinogram OFF-responses Monkey liatraocular Pressure Retinal Pathway

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Differentiation of isolated human umbilical cord mesenchymal stem cells into neural stem cells

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International Journal of ophthalmology(English edition) 2016年第1期9卷 41-47页

作者： Song Chen Wei Zhang Ji-Ming Wang Hong-Tao Duan Jia-Hui Kong Yue-Xin Wang Meng Dong Xue Bi Jian Song Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual Science Tianjin Eye Institute Clinical College of Ophthalmology Tianjin Medical University

AIM：To investigate whether umbilical cord human mesenchymal stem cell（UC-MSC）was able to differentiate into neural stem cell and neuron.·METHODS：The umbilical cords were o btained from pregnant women with their written consent and the approval of the Clinic Ethnics ***-MSC were isolated by adherent culture in the medium contains 20%fetal bovine serum（FBS）,then they were maintained in the medium contain 10%FBS and induced to neural cells in neural differentiation *** investigated whether UC-MSC was able to differentiate into neural stem cell and neuron by using flow cytometry,reverse transcriptase-polymerase chain reaction（RT-PCR）and immunofluorescence（IF）analyzes.·R ESULTS：A substantial number of UC-MSC was harvested using the tissue explants adherent method at about *** cytometric study revealed that these cells expressed common markers of MSCs,such as CD105（SH2）,CD73（SH3）and *** induction of differentiation of neural stem cells,the cells began to form clusters;RT-PCR and IF showed that the neuron specific enolase（NSE）and neurogenic differentiation 1-positive cells reached 87.3%±14.7%and 72.6%±11.8%,*** showed neuronal cell differentiation after induced,including neuron-like protrusions,plump cell body,obviously and stronger ***-PCR and IF analysis showed that microtubule-associated protein 2（MAP2）and nuclear factor-M-positive cells reached 43.1%±10.3%and 69.4%±19.5%,respectively.·CONCLUSION：Human umbilical cord derived MSCs can be cultured and proliferated and differentiate into neural stem cells,which may be a valuable source for cell therapy of neurodegenerative eye diseases.

关键词： human umbilical cord mesenchymal stemcells neural stem cells neuron neurodegenerative eye diseases

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Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma

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International Journal of ophthalmology(English edition) 2016年第12期9卷 1701-1712页

作者： Fang Hou Qi-Ming Huang Dan-Ning Hu Jost B.Jonas Wen-Bin Wei Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology and Visual Science Key Lab Departments of Ophthalmology and Pathology New York Eye and Ear Infirmary of Mount Sinai Department of Ophthalmology Medical Faculty Mannheim of the Ruprecht-Karls-University

AIM： To examine the genetic profile of primary uveal melanoma （UM） as compared to UM in immune ***： Dendritic cells （DC） loaded with lysates of UM cells of high metastatic potential were used to stimulate CTLs（CTLs）. When CTLs co-cultured with the UM cells, most UM cells could be eliminated. Survival UM cells grew slowly and were considered to be survival variants and examined by a microarray analysis. These differential genes were analyzed further with Venn Diagrams and functions related to immune escape. We additionally examined transcriptional changes of manually selected survival variants of UM cells and of clinical UM samples by quantitative real-time polymerase chain reaction （qRT-PCR）, and analyzed the correlation of these expressions and patients’ ***： Gene expression analyses revealed a marked up-regulation of SLAMF7 and CCL22 and a significant down-regulation of KRT10, FXYD3 and ABCC2. The expression of these genes in the relapsed UM was significantly greater than those in primary UM. UM patients with overexpression of these genes had a shorter survival period as compared with those of their ***： Gene expression, in particular of SLAMF7, CCL22, KRT10, FXYD3 and ABCC2, differed between primary UM cells and survival variants of UM cells.

关键词： uveal melanoma dendritic cells immuneescape gene expression profile b＂~2

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The transcorneal electrical stimulation as a novel therapeutic strategy against retinal and optic neuropathy: a review of experimental and clinical trials

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International Journal of ophthalmology(English edition) 2016年第6期9卷 914-919页

作者： Ye Tao Tao Chen Bei Liu Li-Qiang Wang Guang-Hua Peng Li-Min Qin Zhong-Jun Yan Yi-Fei Huang Department of Ophthalmology Ophthalmology & Visual Science Key Lab of PLAGeneral Hospital of Chinese PLA Department of Clinical Aerospace Medicine the Fourth Military Medical University Department of Neurosurgery and Institute for Functional Brain Disorders Tangdu Hospitalthe Fourth Military Medical University

Transcorneal electrical stimulation （TES） is a novel therapeutic approach to activate the retina and related downstream structures. TES has multiple advantages over traditional treatments, such as being minimally invasive and readily applicable in a routine manner. Series of animal experiments have shown that TES protects the retinal neuron from traumatic or genetic induced degeneration. These laboratory evidences support its utilization in ophthalmological therapies against various retinal and optical diseases including retinitis pigmentosa （RP）, traumatic optic neuropathy, anterior ischemic optic neuropathy （AION）, and retinal artery occlusions （RAOs）. Several pioneering explorations sought to clarify the functional mechanism underlying the neuroprotective effects of TES. It seems that the neuroprotective effects should not be attributed to a solitary pathway, on the contrary, multiple mechanisms might contribute collectively to maintain cellular homeostasis and promote cell survival in the retina. More precise evaluations y/a functional and morphological techniques would determine the exact mechanism underlying the remarkable neuroprotective effect of TES. Further studies to determine the optimal parameters and the long-term stability of TES are crucial to justify the clinical significance and to establish TES as a popularized therapeutic modality against retinal and optic neuropathy.

关键词： transcorneal electrical stimulation therapeutic strategy retinal disease optic neuropathy

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Acute Pulmonary Embolism Caused by Local Resection of Choroidal Melanoma

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Chinese Medical Journal 2016年第6期129卷 743-745页

作者： Yao Huang Wen-Bin Wei Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Science Key Lab Beijing 100730 China

Uveal melanoma is the most malignancy in adults, with 6-7 cases/million people.^-~j common primary ocular an annual incidence of The choroid is the most common site of involvement occurring in more than 90% *** We conducted a retrospective study for uveal melanoma at Yongren Eye Center from 1997 to 201 I. A total of 682 cases of uveal melanoma were recorded. Among them, 176 （25.8%） cases received local resection surgery. Most of them survived after surgery and had good recovery. However, life-threatening complication of acute pulmonary embolism （APE） occurred in 3 choroidal melanoma patients shortly after surgery. Till now, to the best of our knowledge, there are few reports of severe postsurgical complications of choroidal melanoma.

关键词： Acute Pulmonary Embolism Choroidal Melanoma Controlled Hypotension Local Resection

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Photonic Crystals: Light‐Patterned Crystallographic Direction of a Self‐Organized 3D Soft Photonic Crystal (Adv. Mater. 42/2017)

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Advanced Materials 2017年第42期29卷

作者： Zhi‐Gang Zheng Cong‐Long Yuan Wei Hu Hari Krishna Bisoyi Ming‐Jie Tang Zhen Liu Pei‐Zhi Sun Wei‐Qiang Yang Xiao‐Qian Wang Dong Shen Yannian Li Fangfu Ye Yan‐Qing Lu Guoqiang Li Quan Li Department of Physics East China University of Science and Technology Shanghai 200237 China Liquid Crystal Institute and Chemical Physics Interdisciplinary Program Kent State University Kent OH 44242 USA National Laboratory of Solid State Microstructures Collaborative Innovation Center of Advanced Microstructures and College of Engineering and Applied Sciences Nanjing University Nanjing 210093 China Beijing National Laboratory for Condensed Matter Physics and CAS Key Laboratory of Soft Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China Visual and Biomedical Optics Lab Department of Ophthalmology and Visual Science and Department of Electrical and Computer Engineering Ohio State University Columbus OH 43212 USA

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Engineered 3D biomembrane using biodegradable PLGA nanofiber scaffold and hiPSC-derived retinal ganglion cells for treatment of Glaucoma

Engineered 3D biomembrane using biodegradable PLGA nanofiber...

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第六届全国眼科学研究生学术论坛暨眼科细胞及再生生物学世界大会

作者： Kangjun Li Xiufeng Zhong Ziming Luo Kang Li Yuchun Liu Chaochao Xu Ying Liu Fei Deng Song Cai Shoutao Lu Haijun Zhang Huaiyu Gu Jian GE Bai Duoan medical equipment company QiheShandongChina Department of Anatomy and Neurobiology Zhongshan School of MedicineYet-sen UniversityGuangzhouGuangdongChina State Key Laboratory of Ophthalmology Key Lab of Ophthalmology and Visual ScienceZhongshan Ophthalmic CenterSun Yet-sen UniversityGuangzhou(510060)GuangdongChina

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A new measure of nystagmus acuity

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International Journal of ophthalmology(English edition) 2014年第1期7卷 95-99页

作者： Jun-Ping Yao Zheng Tai Zheng-Qin Yin Department of Ophthalmology Tianyou HospitalWuhan University of Science and Technology Chongqing Key Lab of Visual Damage and Regeneration & Restoration Chongqing Broadcasting Group Department of Ophthalmology Southwest HospitalThird Military Medical University

AIM: To construct a new visual acuity measuring function for congenital nystagmus(CN) patients by studying the relationships between acuity,velocities and positions of the eye. ·METHODS: After assessing the relationship between acuity,movement velocities and positions of the eye separately,a new function,which we call the automated nystagmus acuity function(ANAF),was constructed to measure the visual acuity of CN patients. Using a high-speed digital video system working at 500 frames per second,each eye was calibrated during monocular fixation. Twenty-six recorded nystagmus data were selected randomly. Using nystagmus waveforms,the best vision position(foveation period) and visual acuity were analyzed in three groups of subjects,and then all outputs were compared with the well-known expanded nystagmus acuity function(NAFX) and ANAF. Standard descriptive statistics were used to summarize the outputs of the two programs. ·RESULTS: Foveation periods were brief intervals in the CN waveform when the image was on or near the fovea and eye velocity was relatively slow. Results showed good visual acuity happened during the period when velocity was low and the eye position was near the zero position,which fitted the foveation periods. The data analyzed with NAFX and ANAF had a correlation coefficient of 0.934276,with an average error of-0.00973. · CONCLUSION: The results from ANAF and NAFX analyses showed no significant difference. The NAFX manually identifies foveation eye positions and produces accurate measurements. The ANAF,however,can be calculated simply using the factors eye position andvelocity,and it automatically calculates the ANAF without the need to manually identify foveation eye positions.

关键词： nystagmus vision acuity nystagmus acuity function eye movement measurements

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Analysis on preliminary results of screening of retinopathy of prematurity from 2008 to 2013 in Tianjin,China

Analysis on preliminary results of screening of retinopathy ...

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2014年斜视与小儿眼科国际高峰论坛

作者： HAN Mei ZHANG Tong-mei LIU Bei HAN Quan-hong Zhao Kanxing Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual ScienceClinical College Of Ophthalmology Tianjin Medical UniversityTianJin300020China

Objective: The aim of the study were to determine the incidence and possible risk factors associated with retinopathy of prematurity(ROP),and explore the impact of pulmonary surfactant Curosurf and Nasal Continuous Positive Airway Pressure(NCPAP)on the development of ROP in Tianjin,***: A multicenter retrospective review was undertaken of the records of 2492 preterm infants in several hospitals of Tianjin,during the period *** were extracted and analysed only in those patients who were born on 37 or less weeks of gestational age.

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Construction of special eye models for investigation of chromatic and higher-order aberrations of eyes

Construction of special eye models for investigation of chro...

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作者： Zhai, Yi Wang, Yan Wang, Zhaoqi Liu, Yongji Zhang, Lin He, Yuanqing Chang, Shengjiang Institute of Modern Optics Nankai University Tianjin300071 China Key Laboratory of Optical Information Science and Technology Ministry of Education Tianjin300071 China Tianjin Eye Hospital and Eye Institute Tianjin Key Lab of Ophthalmology and Visual Science Tianjin Medical University No. 4 Gansu Road Tianjin300020 China

An achromatic element eliminating only longitudinal chromatic aberration (LCA) while maintaining transverse chromatic aberration (TCA) is established for the eye model, which involves the angle formed by the visual and optical axis. To investigate the impacts of higher-order aberrations on vision, the actual data of higher-order aberrations of human eyes with three typical levels are introduced into the eye model along visual axis. Moreover, three kinds of individual eye models are established to investigate the impacts of higher-order aberrations, chromatic aberration (LCA+TCA), LCA and TCA on vision under the photopic condition, respectively. Results show that for most human eyes, the impact of chromatic aberration on vision is much stronger than that of higher-order aberrations, and the impact of LCA in chromatic aberration dominates. The impact of TCA is approximately equal to that of normal level higher-order aberrations and it can be ignored when LCA exists. © 2014 - IOS Press and the authors.

关键词： Optical design

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