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SSRN 2024年

作者： Guan, Anqi Wang, Mengmeng Gong, Yashi Huang, Tiantian Du, Yuguang Zong, Shuai College of Food Science and Engineering Yangzhou University Yangzhou225127 China Jiangsu Alphay Bio-technology Co. Ltd. Nantong226009 China State Key Laboratory of Biochemical Engineering Key Laboratory of Biopharmaceutical Production & Formulation Engineering PLA Institute of Process Engineering Chinese Academy of Sciences Beijing100190 China

Pleurotus ostreatus (P. ostreatus) is one of the most popular edible mushrooms worldwide and has been proposed as a candidate for human selenium (Se) supplementation. This study developed a Se-biofortification method, and evaluated the effect of 2,4-Dichlorophenoxyacetic acid (2,4-D), Na2SeO3 addition amounts and initial pH on P. ostreatus mycelium growth, nutritional value, and mineral accumulation. The optimal growth conditions were identified as 2,4-D 2.00 mg L-1, Na2SeO3 30.65 mg L-1, and initial pH 5.97 by Box-Behnken design. The optimization resulted in the biomass of 15.75 g L-1 and the organic Se content of 594.85 mg Kg-1, which was 2.50 and 8.03 times higher than the control group, respectively. In parallel, the contents of polysaccharides, proteins, essential amino acids, major functional components, and several minerals in mycelium were increased to varying degrees. Above results indicated that 2,4-D could be an effective stimulant for Se biofortification. © 2024, The Authors. All rights reserved.

关键词： Mycelium

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Research progress of CRISPR/Cas systems in nucleic acid detection of infectious diseases

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iLABMED 2023年第1期1卷 58-74页

作者： Jinying Dong Yuguang Du Lei Zhou National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production&Formulation EngineeringInstitute of Process EngineeringChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina Innovation Academy for Green Manufacture Institute Chinese Academy of SciencesBeijingChina Biosafety Research Center Yangtze River Delta in Zhangjiagang SuzhouChina

Infectious diseases are a serious threat to human health,and accurate,rapid and convenient early detection of pathogens is the first step of active *** that detect pathogens have advanced significantly because of the development of fundamental disciplines and the integration of multidisciplinary *** these technologies,nucleic acid detection technology is preferred because of its rapid measurement,accuracy and high *** CRISPR/Cas system,consisting of Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)and CRISPR‐associated(Cas),is an adaptive immune system that specifically recognizes,binds and cleaves exogenous invasive nucleic *** CRISPR/Cas system is widely found in bacteria and *** have developed nucleic acid detection technologies with single‐molecule sensitivity,single‐base precision specificity,portability and low cost based on the specific cleavage and trans‐cleavage activities of the CRISPR/Cas *** next generation of in‐vitro diagnostics is shifting to nucleic acid technology because this technology shows promise in a wide range of applications in resource‐constrained *** this review,the development and mechanism of the CRISPR/Cas system are presented together with representative CRISPR/Cas applications in nucleic acid ***,the review summarizes future perspectives and trends of the CRISPR/Cas system in nucleic acid detection.

关键词： CRISPR/Cas system nucleic acid fluorescence electrochemical chromogenic

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Sprayed Pickering emulsion with high antibacterial activity for wound healing

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Progress in Natural Science:Materials International 2020年第5期30卷 669-676页

作者： Xieru Bao Jie Wu Guanghui Ma State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences School of Chemical Engineering University of Chinese Academy of Sciences PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering Institute of Process Engineering Chinese Academy of Sciences Jiangsu National Synergetic Innovation Center for Advanced Materials

Effective treatment of wound injuries can prevent infections and promote healing. In this work, a sprayed Pickering emulsion composed of chitosan nanoparticles, tea tree oil and curcumin was formulated. The shear thinning behaviour allowed this Pickering emulsion to spray even with a high viscosity provided by the aqueous phase containing chitosan nanoparticles. Moreover, the Pickering emulsion could recover to the high viscosity after spraying onto the wounds, leading to the long residence time and intimate contact. In addition, the Pickering emulsion provided a carrier to encapsulate the tea tree oil, which caused sustained-release effects and further strengthened tea tree oil’s antibacterial activity towards the Gram-negative Escherichia coli(E. coli),Gram-positive Staphylococcus aureus(S. aureus) and Gram-positive methicillin-resistant Staphylococcus aureus(MRSA). Besides, this multi-phase system offered a platform to load with hydrophobic curcumin, leading to better synergistic healing effects. Meanwhile, the Pickering emulsion avoided the irritation and toxicity related to surfactants, which was far safer than the classical emulsion especially when used in the defective skin. All these excellent properties endowed this sprayed Pickering emulsion with promising prospects for wound healing.

关键词： Pickering emulsion Wound healing Chitosan nanoparticles High viscosity Antibacterial activity

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Recent research and development of PLGA/pla microspheres/nanoparticles: A review in scientific and industrial aspects

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Frontiers of Chemical Science and engineering 2019年第1期13卷 14-27页

作者： Feng Qi Jie Wu Hao Li Guanghui Ma Beijing Nuokangda Pharmaceutical Technology Co. Ltd Beijing 100176 China Department of Mechanical and Aerospace Engineering University of Missouri MO 65211 USA National Key Laboratory of Biochemical Engineering Key Laboratory of Biopharmaceutical Production and Formulation Engineering PLA Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 China

Poly(D,L-lactic-co-glycolic acid)(PLGA)/poly (lactic acid)(pla) microspheres/nanoparticles are one of the most successful drug delivery systems (DDS) in lab and clinic. Because of good biocompatibility and biodegradability, they can be used in various areas, such as longterm release system, vaccine adjuvant, tissue engineering, etc. There have been 15 products available on the US market, but the system still has many problems during development and manufacturing, such as wide size distribution, drug stability issues, and so on. Recently, many new and modified methods have been developed to overcome the above problems. Some of the methods are easy to scale up, and have been available on the market to achieve pilot scale or even industrial production scale. Furthermore, the relevant FDA guidance on the DDS is still incomplete, especially for abbreviated new drug application. In this review, we present some recent achievement of the PLGA/pla microspheres/nanoparticles, and discuss some promising manufacturing methods. Finally, we focus on the current FDA guidance on the DDS. The review provides an overview on the development of the system in pharmaceutical industry.

关键词： PLGA microspheres drug delivery system stability manufacturing

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A Bioinspired Microfluidic Nucleic Acid Sensing platform for Rapid and Simultaneous Screening of Viral Respiratory Infections

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ACS sensors 2025年 2025 May 29页

作者： Xiaoya Wu Yan Su Yue Xu Qiushi Hu Juan Jiao Jiali Ma Xubo Liu Chongsi Sun Wei Guo Lei Zhou State Key Laboratory of Biopharmaceutical Preparation and Delivery PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 People's Republic of China. University of Chinese Academy of Sciences Beijing 100049 People's Republic of China. Department of Physics Capital Normal University Beijing 100048 People's Republic of China. Biosafety Research Center Yangtze River Delta in Zhangjiagang Suzhou 215611 People's Republic of China. Department of Clinical Laboratory Seventh Medical Center of PLA General Hospital Beijing 100007 People's Republic of China.

In the postpandemic era, to tackle the engineering challenge from the concurrent outbreak of routine and emerging viral respiratory infections, microfluidic-chip-based point-of-care screening of multiple pathogens becomes essential for decentralized epidemic diagnosis and management. Toward real-world POCT, state-of-the-art microfluidic diagnostic devices are limited by the in-plane design of the fluidic circuit, which highly relies on external pumping apparatus for sample injection and distribution and thus greatly hampers their portability and accessibility. In this work, inspired by the pump-free water absorption mechanism of plant root hairs, we report a self-powered hand-held microfluidic sensing platform for simultaneous and rapid nucleic acid screening of viral respiratory pathogens, such as influenza A and B and SARS-CoV-2. This design lifts the liquid distribution port out of the principal plane of the microfluidic circuit to harness the power of gravity for sample injection and equal distribution. The root-hair-like liquid distribution channels enable fast and directional transport of limited biological specimen. Cascaded recombinase polymerase amplification and lateral flow assay yield rapid visual readout for the three pathogens in 40 min with a competitive detection sensitivity as low as 0.18 copies/μL, close to the detection limit of qPCR (gold standard). For real clinical specimens, retrospective research shows a sensitivity of 93.2% and a specificity of 97.7%. From an economic perspective, a unit cost down to 1.4 USD per test can generally be attained. This design leverages biomimetic structures to boost pump-free liquid transport for rapid and cost-efficient POCT for decentralized diagnostics, particularly in infrastructure-deficient areas.

关键词： POCT biomimetic structures microfluidics nucleic acid detection pathogen screening

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The Emulsion Particulate Strategy: The Horizon of the Emulsion Particulate Strategy: engineering Hollow Particles for Biomedical Applications (Adv. Mater. 38/2019)

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Advanced Materials 2019年第38期31卷

作者： Yufei Xia Xiangming Na Jie Wu Guanghui Ma State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 P. R. China School of Chemical Sciences University of Chinese Academy of Sciences Beijing 100049 P. R. China PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 P. R. China Jiangsu National Synergetic Innovation Center for Advanced Materials Nanjing 211816 P. R. China

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Immune Responses: Bridging Systemic Immunity with Gastrointestinal Immune Responses via Oil‐in‐Polymer Capsules (Adv. Mater. 31/2018)

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Advanced Materials 2018年第31期30卷

作者： Yufei Xia Jie Wu Yiqun Du Chunyu Miao Zhiguo Su Guanghui Ma State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 P. R. China University of Chinese Academy of Sciences Beijing 100049 P. R. China PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 P. R. China Ministry of Education Key Laboratory of Carbohydrate Chemistry and Biotechnology School of Biotechnology Jiangnan University Wuxi 214122 P. R. China Jiangsu National Synergetic Innovation Center for Advanced Materials Nanjing 211816 P. R. China

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Chitosan Oligosaccharide Inhibits Bacteria Biofilm Through Interfering Polysaccharide Structure and Biosynthesis

Chitosan Oligosaccharide Inhibits Bacteria Biofilm Through I...

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中国化学会·2016全国多糖研讨会

作者： Zhuo Wang Yuguang Du State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of Sciences Key Laboratory of Biopharmaceutical Production &FormulationEngineering PLA

With the widespread use of antibiotics,pathogens have been becoming increasingly serious because of drug *** studies found that the resistance of pathogens to antimicrobial drugs is closely related to the formation of biofilms,and more than 80%of human bacterial infections are associated with *** is an existence formation of bacteria for self-protection,and the bacteria formed biofilm was considerably more resistant to antibiotics than planktonic *** greatly hamper the effect of antibiotic treatment on bacterial infection,anti-biofilm drugs are required to treat biofilm-related *** we demonstrate the anti-biofilm effect of chitosan oligosaccharide(COS) on Pseudomonas aeruginosa and Staphylococcus ***,the anti-biofilm activity of COS against bacteria biofilm is related to the degree of deacetylation and polymerization,although the positive charge of COS is not necessary for its anti-biofilm *** study suggested that COS caused disorganization of the biofilm matrix through interfering polysaccharide structures of biofilm as well as manipulating expression level of genes related to biofilm polysaccharide ***,this work present an innovative strategy and theoretical basis to treating recalcitrant bacterial infections.

关键词： Chitosan Oligosaccharide Inhibits Bacteria Biofilm Through Interfering Polysaccharide Structure and Biosynthesis

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Uniform-sized PLGA-HSPC lipospheres as a promising platform for oral delivery of proteins and peptides

Uniform-sized PLGA-HSPC lipospheres as a promising platform ...

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2015年中国药物制剂大会暨中国药学会药剂专业委员会2015年学术年会暨国际释控协会中国分会2015年学术年会

作者：杨婷媛 Tongtong Ma Lianyan Wang Guanghui Ma National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production & Formulation EngineeringInstitute of Process EngineeringChinese Academy of Sciences

With the rapid development of biotechnology and genetic engineering,an increasing number of potential therapeutic proteins and peptides have been ***,most protein molecules are easily degraded by proteases before they are delivered to the site of action,resulting in short half-life in vivo and multiple *** order to overcome these limitations,sustained and controlled release formulations based on natural macromolecules and polymers were developed,including liposomes,micro-particles and so *** now,it still exists big challenges to increase the encapsulation efficiency（EE）,maintain the bioactivity of proteins and release the loaded proteins in a sustained and controlled *** designed biodegradable polymeric lipospheres which were composed of a hydrophobic polymeric core and a surface coating of lipid layer,and this structure was validated by transmission electron microscopy（TEM） and confocal laser scanning microscopy（CLSM） .During the formation of lipospheres,the amphiphilic phospholipids acted as a co-emulsifier and the lipid molecules self-assembled at the interface of W/O and O/W,which not only prevented proteins from denaturation but also achieved high entrapment efficiency（90.82±2.39%）.Furthermore,the lipid-shell on the surface of lipospheres greatly reduced initial burst（24.73±1.32%） and maintained protein release in sustained *** addition,the size of uniform-sized lipospheres could be well controlled by employing premix membrane emulsification method by using membrane with different pore size.

关键词： Lipospheres Premix membrane emulsification Controlled delivery Proteins and peptides

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Uniform-sized PLGA-HSPC lipospheres as a promising platform for oral delivery of proteins and peptides

Uniform-sized PLGA-HSPC lipospheres as a promising platform ...

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The 6th International Conference on Nanoscience & Technology, China 2015

作者： Tingyuan Yang Lianyan Wang Guanghui Ma National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production ＆ Formulation EngineeringInstitute of Process EngineeringChinese Academy of Sciences

With the rapid development of biotechnology and genetic engineering, an increasing number of potential therapeutic proteins and peptides have been discovered. However, most protein molecules are easily degraded by proteases before they are delivered to the site of action, resulting in short half-life in vivo and multiple injections. In order to overcome these limitations, sustained and controlled release formulations based on natural macromolecules and polymers were developed, including liposomes, micro-particles and so on. Until now, it still exists big challenges to increase the encapsulation efficiency（EE）, maintain the bioactivity of proteins and release the loaded proteins in a sustained and controlled manner. We designed biodegradable polymeric lipospheres which were composed of a hydrophobic polymeric core and a surface coating of lipid layer, and this structure was validated by transmission electron microscopy（TEM） and confocal laser scanning microscopy（CLSM） [1]. During the formation of lipospheres, the amphiphilic phospholipids acted as a co-emulsifier and the lipid molecules self-assembled at the interface of W1/O and O/W2, which not only prevented proteins from denaturation but also achieved high entrapment efficiency（90.82±2.39%）. Furthermore, the lipid-shell on the surface of lipospheres greatly reduced initial burst（24.73±1.32%） and maintained protein release in sustained manner. In addition, the size of uniform-sized lipospheres could be well controlled by employing premix membrane emulsification method by using membrane with different pore size.

关键词： Lipospheres Premix membrane emulsification Controlled delivery Proteins and peptides

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