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Proteomic profiling of tumor microenvironment and prognosis risk prediction in stage I lung adenocarcinoma

Lung Cancer 2024年 191卷 107791-107791页

作者： Lu, Yueh-Feng Chang, Ya-Hsuan Chen, Yi-Ju Hsieh, Min-Shu Lin, Mong-Wei Hsu, Hsao-Hsun Han, Chia-Li Chen, Yu-Ju Yu, Sung-Liang Chen, Jin-Shing Chen, Hsuan-Yu Ph.D. Program in Translational Medicine National Taiwan University and Academia Sinica Taiwan Division of Radiation Oncology Department of Radiology Far Eastern Memorial Hospital New Taipei City Taiwan Institute of Molecular and Genomic Medicine National Health Research Institute Taiwan Institute of Chemistry Academia Sinica Taipei Taiwan Department of Pathology National Taiwan University Hospital and National Taiwan University College of Medicine Taipei Taiwan Department of Surgery National Taiwan University Hospital and National Taiwan University College of Medicine Taipei Taiwan Master Program in Clinical Genomics and Proteomics College of Pharmacy Taipei Medical University Taipei Taiwan Department of Clinical Laboratory Sciences and Medical Biotechnology National Taiwan University Taiwan Doctoral Program in Microbial Genomics National Chung Hsing University and Academia Sinica Taiwan Center for Cancer Research Kaohsiung Medical University Taiwan Institute of Statistical Science Academia Sinica Taipei Taiwan

Objectives: With the increasing popularity of CT screening, more cases of early-stage lung cancer are being diagnosed. However, 24.5% of stage I non-small-cell lung cancer (NSCLC) patients still experience treatment failure post-surgery. Biomarkers to predict lung cancer patients at high risk of recurrence are needed. Materials and methods: We collected protein mass spectrometry data from the Taiwan Lung Cancer Moonshot Project and performed bioinformatics analysis on proteins with differential expressions between tumor and adjacent normal tissues in 74 stage I lung adenocarcinoma (LUAd) cases, aiming to explore the tumor microenvironment related prognostic biomarkers. Findings were further validated in 6 external cohorts. Results: The analysis of differentially expressed proteins revealed that the most enriched categories of diseases and biological functions were cellular movement, immune cell trafficking, and cancer. Utilizing proteomic profiling of the tumor microenvironment, we identified five prognostic biomarkers (AdAM10, MIF, TEK, THBS2, MAOA). We then developed a risk score model, which independently predicted recurrence-free survival and overall survival in stage I LUAd. Patients with high risk scores experienced worse recurrence-free survival (adjusted hazard ratio = 8.28, p < 0.001) and overall survival (adjusted hazard ratio = 6.88, p = 0.013). Findings had been also validated in the external cohorts. Conclusion: The risk score model derived from proteomic profiling of tumor microenvironment can be used to predict recurrence risk and prognosis of stage I LUAd. © 2024 Elsevier B.V.

关键词： Lung adenocarcinoma Mass spectrometry Prognostic biomarker Proteomic Tumor microenvironment

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Evaluating the antiviral efficacy and specificity of chlorogenic acid and related herbal extracts against SARS-CoV-2 variants via spike protein binding intervention

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Journal of Traditional and Complementary Medicine 2024年

作者： Hsieh, Wen-Yu Yu, Chu-Nien Chen, Chang-Chang Chiou, Chun-Tang Green, Brian d. Lee, Oscar K. Wu, Chia-Chune doan, Ly Hien Huang, Chi-Ying F. Huang, Cheng Liu, Chien-Ju Chen, Yu-Hsin Cheng, Jing-Jy Pan, Heng-Chih Liu, Hui-Kang Biomedical Industry Ph.D. Program National Yang Ming Chiao Tung University Taipei 11221 Taiwan Institute of Clinical Medicine National Yang Ming Chiao Tung University Taipei 11221 Taiwan Division of Chinese Materia Medica Development National Research Institute of Chinese Medicine Ministry of Health and Welfare Taipei 11221 Taiwan Institute for Global Food Security School of Biological Sciences Queen's University Belfast Belfast BT7 1NN United Kingdom Department of Orthopedics China Medical University Hospital Taichung Taiwan Institute of Biopharmaceutical Sciences College of Pharmaceutical Sciences National Yang Ming Chiao Tung University Taipei 11221 Taiwan Institute of Biotechnology Vietnam Academy of Science and Technology Hanoi Viet Nam Program in Molecular Medicine College of Life Sciences National Yang Ming Chiao Tung University Taipei 11221 Taiwan Taiwan National Graduate Program in Molecular Medicine Academia Sinica National Yang Ming Chiao Tung University Taipei 11221 Taiwan Institute of Clinical Medicine College of Medicine National Yang Ming Chiao Tung Taipei 11221 Taiwan Department of Biotechnology and Laboratory Science in Medicine School of Biomedical Science and Engineering National Yang Ming Chiao Tung Taipei 11221 Taiwan Department of Biochemistry School of Medicine Kaohsiung Medical University Kaohsiung 80708 Taiwan Department of Earth and Life Sciences University of Taipei Taipei 11036 Taiwan Tea and Beverage Research Station Ministry of Agriculture Yangmei Township Taoyuan County 326011 Taiwan Taichung District Agricultural Research and Extension Station Ministry of Agriculture Chang-Hwa County 515008 Taiwan Division of Basic Chinese Medicine National Research Institute of Chinese Medicine Ministry of Health and Welfare Taipei 11221 Taiwan Department of Nephrology Chang Gung Memorial Hospital Keelung City 204201 Taiwan Ph.D. Program in the Clinical Drug Development of Herbal Medicine Taipei Medical University Taipei

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus infect the respiratory tract through surface proteins, causing similar symptoms. Since the onset of the COVId-19 pandemic, both viruses have posed significant ongoing global health threats. Like the influenza virus, SARS-CoV-2 evolves into variants that can reduce vaccine efficacy. Thus, herbal medicines are being explored as supplementary options to enhance protection against these infections. This study aimed to investigate the therapeutic potential of chlorogenic acid (3-CQA) and related extracts from green coffee beans and Echinacea purpurea against SARS-CoV-2 variants and H1N1 infection. The methods employed included an ELISA-based trimeric spike protein binding assay, viral infection assays, plaque assays, and molecular docking studies. Results showed that 3-CQA blocked spike protein/angiotensin converting enzyme 2 (ACE2) binding for most SARS-CoV-2 variants of concern, except the delta variant. Extracts from Green coffee bean and E. purpurea effectively blocked all variants tested. Additionally, antibodies blocked spike protein binding up to Omicron BA.2. Molecular docking suggested that 3-CQA binding to Omicron BA.1, BA.2, and BA.4, though not to the delta spike protein, may lead to steric hindrance, preventing receptor-binding domain interactions with ACE2. Finally, both 3-CQA and E. purpurea extract showed preventive effects against H1N1 viral infection, though with lower potency compared to SARS-CoV-2. In conclusion, 3-CQA has potential as a phytoconstituent marker for herbs with bioactive properties against SARS-CoV-2 and H1N1 viral infections. © 2024 Center for Food and Biomolecules, National Taiwan University

关键词： Chlorogenic acid COVId-19 Echinacea purpurea Green coffee bean H1N1 influenza virus SARS-CoV-2 variants Spike protein

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Improvement of extracellular polysaccharides production from Cordyceps militaris immobilized alginate beads in repeated-batch fermentation

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LWT 2024年 193卷

作者： Hou, Shi-Jing Cheng, Kuan-Chen Lin, Shyh-Hsiang Hsiao, I-Lun Santoso, Shella Permatasari Singajaya, Stephanie Chou, Yu-Chieh Lin, Shin-Ping Master Program in Food Safety Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Institute of Biotechnology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei10617 Taiwan Graduate Institute of Food Science and Technology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei10617 Taiwan Department of Medical Research China Medical University Hospital China Medical University 91 Hsueh-Shih Rd. Taichung40402 Taiwan Department of Optometry Asia University 500 Lioufeng Rd. Wufeng Taichung41354 Taiwan School of Nutrition and Health Sciences Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan School of Food Safety Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Department of Chemical Engineering Widya Mandala Surabaya Catholic University Kalijudan 37 East Java Surabaya60114 Indonesia Collaborative Research Center for Sustainable and Zero Waste Industries Kalijudan 37 East Java Surabaya60114 Indonesia Ph.D. Program in Drug Discovery and Development Industry Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Research Center of Biomedical Device Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan TMU Research Center for Digestive Medicine Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan

Cordyceps militaris (C. militaris) is a unique medicinal fungus of the genus Cordyceps. It has high economic value due to various biological functions from the extracellular polysaccharides (EPSs) it produces. The aim of this study was to establish a mycelium-immobilization system for EPS production in a bubble column system. First, EPS production was optimized by 90.2% (from 0.72 to 1.37 g/L) using response surface methodology under an alginate-immobilized mycelium system with 6 days of cultivation. The highest EPS production was obtained (5.3 g/L) in a flask system through elongation to 15 days of cultivation. EPS production subsequently further increased to 10.42 g/L utilizing a bubble column reactor. Furthermore, the system can be reused for at least three rounds. In the EPS characteristics analysis, the produced EPSs were mainly composed of glucose and galactose. They presented strong antibacterial activity against Staphylococcus aureus. In terms of antioxidant activity, respective the EPS concentration that caused 50% scavenging of dPph and ABTS free radicals values (IC50) were 4.65 and 7.92 mg/mL. In summary, these findings should be helpful for establishing a suitable industrial fermentation system for C. militaris and can be studied in the food and medical industries in future work. © 2024 The Authors

关键词： Mycelium

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Ultrasensitive detection of ornidazole–antibiotic drug residues in water using NiMOFs functionalized MWCNT modified electrode

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Microchemical Journal 2024年 197卷

作者： Vignesh, Kondusamy Senthil Kumar, Annamalai Arumugam Napoleon, Ayyakannu Kuo, Chih-Yu Yusuf, Kareem Govindasamy, Mani Nano and Bioelectrochemistry Research Laboratory Carbon dioxide Research and Green Technology Centre and Department of Chemistry School of Advanced Sciences Vellore Institute of Technology Vellore 632 014 India Department of Chemistry School of Advanced Sciences Vellore Institute of Technology Vellore 632 014 India International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices Ming Chi University of Technology New Taipei City 243303 Taiwan Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei 10608 Taiwan Department of Chemistry College of Science King Saud University Riyadh 11451 Saudi Arabia Centre for Applied Research Saveetha School of Engineering Saveetha Institute of Medical and Technical Sciences (SIMATS) Tamil Nadu Chennai 602105 India Research Center for Intelligence Medical Devices Ming Chi University of Technology New Taipei City 243303 Taiwan

Nitroimidazole-based drugs are widely used as an antibiotic for the treatment of anaerobic bacterial and parasite illnesses. The widespread use of these drugs in animal feed and the consequent environmental pollution have become significant concerns in recent years. Simple, sensitive, and rapid detection of the antibody in various real samples is demanding research interest. Herein, we report a Nickel-metal organic framework functionalized Multiwalled Carbon nanotubes modified glassy carbon electrode (MWCNT@NiMOFs) as an ultrasensitive and rapid voltammetric sensor for the detection of ornidazole (ORdZ) in a neutral ph solution. The MWCNT@NiMOFs were prepared using a microwave-assisted solvothermal technique accompanied by an ultrasonication method. The designed MWCNT@NiMOFs offered a low charge transfer resistance (R_ct) of 141 Ω along with excellent electrocatalytic activity, and high current density at the lowered cathodic peak potential (-0.62 V vs Ag/AgCl) for ORdZ detection. The surface adsorption capacity (Γ_s) in terms of surface excess and heterogeneous charge transfer rate constant (k⁰) of the MWCNT@NiMOFs were calculated to be 3.29 × 10^-9 mol cm⁻² and 5.33 × 10^-3 cm s⁻¹, respectively. Under an optimal working condition, differential pulse voltammetry measurements of ORdZ have shown concentration linearity in a window, 100 nm to 10 µM range with excellent sensitivity, 5.69 × 10^-5A µM⁻¹ cm⁻², and detection limit, 26 nM (3.3/s). The designed MWCNT@NiMOFs offered appreciable reproducibility, and repeatability up to 30 days of storage time. The real-time quantitative evaluation of ORdZ in human urine and tap water specimens was done along with ∼ 100 % recovery values. Since the new approach is simple, selective, and separation-free, it can be extendable to a variety of real-time practical applications. © 2024 Elsevier B.V.

关键词： Electrocatalytic reduction Multi-walled carbon nanotubes Nickel Metal-organic frameworks Ornidazole drug Ultrasensitive detection

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Astrocytic Cebpd Regulates Pentraxin 3 Expression to Promote Fibrotic Scar Formation After Spinal Cord Injury

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Molecular neurobiology 2023年第4期60卷 2200-2208页

作者： Shao-Ming Wang Jung-Yu C Hsu Chiung-Yuan Ko Hsiang-En Wu Yu-Wei Hsiao Ju-Ming Wang Graduate Institute of Biomedical Sciences China Medical University Taichung 404333 Taiwan. wangs@mail.cmu.edu.tw. Neuroscience and Brain Disease Center China Medical University Taichung Taiwan. wangs@mail.cmu.edu.tw. Department of Neurology China Medical University Hospital Taichung Taiwan. wangs@mail.cmu.edu.tw. Department of Cell Biology and Anatomy College of Medicine National Cheng Kung University Tainan Taiwan. Ph.D. Program in Medical Neuroscience College of Medical Science and Technology Taipei Medical University Taipei Taiwan. TMU Research Center of Neuroscience Taipei Medical University Taipei Taiwan. TMU Research Center of Cancer Translational Medicine Taipei Taiwan. Cellular Pathobiology Section Integrative Neuroscience Research Branch Intramural Research Program National Institute on Drug Abuse NIH/DHHS Suite 3512 333 Cassell Drive Baltimore MD 21224 USA. Department of Biotechnology and Bioindustry Sciences College of Bioscience and Biotechnology National Cheng Kung University Tainan Taiwan. Department of Biotechnology and Bioindustry Sciences College of Bioscience and Biotechnology National Cheng Kung University Tainan Taiwan. yumingw@mail.ncku.edu.tw. Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei Taiwan. yumingw@mail.ncku.edu.tw. International Research Center for Wound Repair and Regeneration National Cheng Kung University Tainan Taiwan. yumingw@mail.ncku.edu.tw. Graduate Institute of Medicine College of Medicine Kaohsiung Medical University Kaohsiung Taiwan. yumingw@mail.ncku.edu.tw. Institute of Bioinformatics and Biosignal Transduction College of Bioscience and Biotechnology National Cheng Kung University Tainan 701 Taiwan. yumingw@mail.ncku.edu.tw.

Astroglial-fibrotic scars resulted from spinal cord injury affect motor and sensory function, leading to paralysis. In particular, the fibrotic scar is a main barrier that disrupts neuronal regeneration after spinal cord injury. However, the association between astrocytes and fibrotic scar formation is not yet understood. We have previously demonstrated that the transcriptional factor Cebpd contributes to astrogliosis, which promotes glial scar formation after spinal cord injury. Herein, we show that fibrotic scar formation was decreased in the epicenter region in Cebpd mice after contusive spinal cord injury and astrocytic Cebpd promoted fibroblast migration through secretion of Ptx3. Furthermore, the expression of Mmp3 was increased under recombinant protein Ptx3 treatment in fibroblasts by observing microarray data, resulting in fibroblast migration. In addition, regulation of Mmp3 occurs through the NFκB signaling pathway by using an irreversible inhibitor of IκBα phosphorylation in pretreated fibroblasts. Of note, we used the synthetic peptide RI37, which blocks fibroblast migration and decreases fibroblast Mmp3 expression in IL-1β-treated astrocyte conditioned media. Collectively, our data suggest that fibroblast migration can be affected by astrocytic Cebpd through the Ptx3/NFκB/Mmp3 axis pathway and that the RI37 peptide may act as a therapeutic medicine to inhibit fibrotic scar formation after spinal cord injury.

关键词： Astrocyte Cebpd Fibrotic scar Ptx3 Spinal cord injury

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Integration proteomics analysis to identify AMPK as key target pathways of TCM formula for high fat diet induced obesity in mice

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Journal of Traditional and Complementary Medicine 2025年

作者： Chen, Yu-Ju Ning, de-Shan Wang, Ching-Chiung Zhao, Hong-Wei Wang, Kun-Teng Lee, Ming-Chung Chiu, Wan Chun Yeh, Chiu-Li Jacinto dela Vega, John Louie Lee, Chia-Jung Ph.D. Program in Clinical Drug Development of Herbal Medicine College of Pharmacy Taipei Medical University Taipei Taiwan Infinitus Company Ltd Guangdong Guangzhou China Graduate Institute of Pharmacognosy College of Pharmacy Taipei Medical University Taipei Taiwan School of Pharmacy Collage of Pharmacy Taipei Medical University Taipei Taiwan Traditional Herbal Medicine Research Center Taipei Medical University Hospital Taipei Taiwan Herbiotek Co. Ltd New Taipei City Taiwan School of Nutrition and Health Sciences College of Nutrition Taipei Medical University Taipei Taiwan Ph.D. Program in School of Pharmacy College of Pharmacy Taipei Medical University Taipei Taiwan

Background: Livsooth Authentic Herbal Formula (LAH) is a novel Chinese herbal medicine that has been previously shown to prevent non-alcoholic fatty liver disease (NAFLd). However, its efficacy in treating obesity and its underlying mechanisms remain unclear. This study uniquely investigates the therapeutic effects of LAH on high-fat diet (HFd)-induced obese mice, focusing on its multi-targeted regulation of metabolic pathways. This research highlights the potential of a multi-component herbal formula in simultaneously activating the AMPK pathway, regulating lipid metabolism, and enhancing antioxidant defenses. By integrating network pharmacology predictions with proteomics analysis, in vivo, and in vitro experiments, this study provides a comprehensive understanding of LAH's mode of action. Materials and methods: Mice were fed a high-fat diet (HFd) for 8 weeks, followed by oral treatment with LAH at doses of 615 mg/kg and 2460 mg/kg for 10 weeks. Each treatment group consisted of 6 mice. Body weight, blood biochemistry, and antioxidant enzyme activities were measured. Network pharmacology and proteomics analyses were conducted to identify mechanisms, and in vitro studies validated molecular pathways. Results: This study utilized network pharmacology to investigate the therapeutic effects and mechanisms of LAH on obesity. Through relevant databases, 19 major chemical components and 605 potential targets were identified. KEGG pathway analysis identified the AMPK signaling pathway as a key target of LAH. Animal experiments showed that LAH reduced body weight by 16.55 % compared to HFd-induced mice. In addition to weight reduction, LAH significantly improved serum metabolic parameters. Glucose, triglyceride, and cholesterol levels were significantly reduced, and liver function improved, with ALT decreasing from 142.00 ± 32.63 U/L (HFd) to 63.57 ± 33.16 U/L (H-LAH), and AST from 147.20 ± 12.92 U/L (HFd) to 81.71 ± 31.31 U/L (H-LAH). It also enhanced liver antioxidant en

关键词： AMPK pathway Herb-based supplements Network pharmacology Obesity Proteomics analysis

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Overall mortality risk analysis for rectal cancer using deep learning-based fuzzy systems

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Computers in Biology and Medicine 2023年 157卷 106706-106706页

作者： Yang, Cheng-Hong Chen, Wen-Ching Chen, Jin-Bor Huang, Hsiu-Chen Chuang, Li-Yeh Department of Information Management Tainan University of Technology Tainan Taiwan Department of Electronic Engineering National Kaohsiung University of Science and Technology Kaohsiung Taiwan Ph. D. Program in Biomedical Engineering Kaohsiung Medical University Kaohsiung Taiwan School of Dentistry Kaohsiung Medical University Kaohsiung Taiwan Drug Development and Value Creation Research Center Kaohsiung Medical University Kaohsiung Taiwan Department of Internal Medicine Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine Kaohsiung Taiwan Department of Community Health Chia-Yi Christian Hospital Chia-Yi City Taiwan Department of Chemical Engineering & Institute of Biotechnology and Chemical Engineering I-Shou University Kaohsiung Taiwan

Colorectal cancer is a leading cause of cancer mortality worldwide, with an increasing incidence rate in developing countries. Integration of genetic information with cancer therapy guidance has shown promise in cancer treatment, indicating its potential as an essential tool in translation oncology. However, the high-throughput analysis and variability of genomic data poses a major challenge to conventional analytic approaches. In this study, we propose an advanced analytic approach, named "Fuzzy-based RNNCoxph," incorporated fuzzy logic, recurrent neural networks (RNNs), and Cox proportional hazards regression (Coxph) for detecting missense variants associated with high-risk of all-cause mortality in rectum adenocarcinoma. The test data set was downloaded from "Rectum adenocarcinoma, TCGA-REAd" the Genomic data Commons (GdC) portal. In this study, four model-based risk score models were derived using RNN, Coxph, RNNCoxph.d.ition, and RNNCoxphMultiplication. The RNNCoxph.d.ition and RNNCoxphMultiplication models were obtained as the sum and product of the RNN risk degree matrix and the Coxph risk degree matrix, respectively. Moreover, the fuzzy logic system was used to calculate the survival risk values of missense variants and classified their membership grade to improve the identification of high-risk gene variation locations associated with cancer mortality. The four models were integrated to develop an advanced risk estimation model. There were 20 028 variants associated with survival status, amongst 17 638 variants were associated with survival and 2390 variants associated with mortality. The proposed Fuzzy-based RNNCoxph model obtained a balanced accuracy of 93.7%, which was significantly higher than that of the other four test methods. In particular, the Coxph model is commonly used in medical researches and the XGBoost model is famous for its high accuracy in machine learning. The results suggest that the Fuzzy-based RNNCoxph model exhibits a higher efficacy

关键词： Fuzzy logic

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CoAl2O4 nanoparticles modified carbon nanofibers as high-efficiency bifunctional electrocatalyst: An efficient electrochemical aqueous asymmetric supercapacitors and non-enzymatic electrochemical sensors

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Journal of Alloys and Compounds 2023年 931卷 1页

作者： Chinnapaiyan, Sathishkumar das, Himadri Tanaya Chen, Shen-Ming Govindasamy, Mani Alshgari, Razan A. Fan, Chi-Hsuan Huang, Chi-Hsien Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei10608 Taiwan Centre for Advanced Materials and Applications Utkal University Vani vihar Odisha Bhubaneswar751004 India Department of Materials Engineering Ming Chi University of Technology New Taipei City243303 Taiwan International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices Ming Chi University of Technology New Taipei City243303 Taiwan Chemistry Department College of Science King Saud University Riyadh11451 Saudi Arabia Korea University of Technology and Education Chungcheongnam-do Cheonan-si31253 Korea Republic of Division of Rheumatology Allergy and Immunology Chang Gung Memorial Hospital Taoyuan33378 Taiwan Research Center for Intelligent Medical Devices Ming Chi University of Technology New Taipei City243303 Taiwan

The thriving energy demand of the gradually increasing population and modernized life style requires development in energy storage devices for usage in commercial electronic devices. In this particular work, we have explored electrochemical performances of bimetallic oxide CoAl2O4 and tuned its performance by decorating it on the carbon nanofibers. The CoAl2O4/CNFs are synthesized by deep eutectic solvents based hydrothermal method. The synthesized CoAl2O4 nanoparticle was found electrochemically active and the incorporation of CNFs end up boosting, its electrochemical performances as battery-type electrode. The obtained nanocomposites were characterized by various techniques. The electrochemically active CoAl2O4/CNFs exhibited 2.2-fold higher charge storage capacity than CoAl2O4. The CoAl2O4/CNFs exhibited specific capacity as high as 132 C/g at a constant applied current density of 1 A/g in 1 M KOH. The asymmetric device fabricated with CoAl2O4/CNFs electrodes exhibited excellent energy density of 20 Wh/kg at power density of 3000 W/kg. The CoAl2O4/CNFs electrode long-term stability has been minutely observed;the high Coulombic efficiency of 90% and high rate of capacity retention 95 %. In this work, an enzyme-free electrochemical sensor based on CoAl2O4/CNFs composite was developed for the detection of luteolin (LUT). Further modification of CoAl2O4/CNFs composite can improve the high surface area and catalytic capability of the composites. Under the optimal detection conditions, the prepared sensor has good linearity in the detection of LUT within range 30 nM − 73.5 μM with a limit of detection (LOd) of 0.0124 μM (12.4 nM). The stability and selectivity of the sensor is high, and can be used to detect LUT in various real samples. © 2022 Elsevier B.V.

关键词： Electrochemical sensors

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Effects of Citrus depressa Hayata juice on high-fat diet-induced obesity in HBV transgenic mice

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Heliyon 2024年第2期10卷

作者： Chu, Pei-Yi Hsu, Chang-Lu Lin, Yen-An Pan, Yi-Cheng dai, Yun-Hao Yu, Ying-Chun Yang, Juan-Cheng Ma, Wen-Lung Chen, Yi-Jinn Lillian Lee, Chia-Lin Wu, Yang-Chang Chinese Medicine Research and Development Center China Medical University Hospital Taichung Taiwan Department of Business Administration National Chiayi University Chiayi Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan .Ph.D. Program for Cancer Biology and Drug Discovery China Medical University and Academia Sinica Taichung Taiwan School of Pharmacy China Medical University Taichung Taiwan Department of Medical Research and Organ Transplantation Center China Medical University Hospital Taichung Taiwan School of Chinese Medicine College of Chinese Medicine China Medical University Taichung Taiwan Department of Physics National Kaohsiung Normal University Kaohsiung Taiwan Department of Cosmeceutics China Medical University Taichung 40604 Taiwan Graduate Institute of Integrated Medicine College of Chinese Medicine China Medical University Taichung Taiwan Department of Medical Laboratory Science and Biotechnology College of Medical and Health Science Asia University Taichung Taiwan

The present study investigated the potential anti-obesity properties of Citrus depressa Hayata (CdH) juice in HBV transgenic mice, as well as the impact of fermentation on the effectiveness of the juice. The results revealed that fermentation increased the levels of polyphenols and hesperidin in CdH juice. The animal study demonstrated that both juices were effective in mitigating the weight gain induced by a high-fat diet by correcting metabolic parameter imbalances, reducing hepatic lipid accumulation, and reversing hepatic immune suppression. Furthermore, fermented juice exhibited superior efficacy in managing body weight and inhibiting the expansion of white adipose tissue (WAT). Fermented juice significantly enhanced adiponectin production and PPARγ expression in WAT, while also reducing hypertrophy. This study offers valuable insights into the potential role of CdH juices in combating obesity associated with high fat consumption and underscores the promise of CdH juice as a functional beverage. © 2024 The Authors

关键词： Citrus depressa hayata fermentation HBV Hesperidin High-fat diet-induced obesity Juice Mice Polyphenol

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Ser235 phosphorylation of hepatitis C virus NS5A is required for NS5A dimerization and drug resistance

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Life Sciences 2024年 337卷 122338页

作者： Lee, Wei-Ping Tsai, Keng-Chang Liao, Shi-Xian Huang, Yi-Hsiang Hou, Ming-Chih Lan, Keng-Hsin Department of Medical Research Taipei Veterans General Hospital Taipei Taiwan Institute of Biochemistry and Molecular Biology School of Life Sciences National Yang Ming Chiao Tung University Taipei Taiwan National Research Institute of Chinese Medicine Ministry of Health and Welfare Taipei Taiwan The Ph.D. Program for Medical Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan Division of Gastroenterology and Hepatology Department of Medicine Taipei Veterans General Hospital Taipei Taiwan School of Medicine College of Medicine National Yang Ming Chiao Tung University Taipei Taiwan Institute of Clinical Medicine School of Medicine National Yang Ming Chiao Tung University Taipei Taiwan Institute of Pharmacology College of Medicine National Yang Ming Chiao Tung University Taipei Taiwan

Hepatitis C virus (HCV) infection is recognized as a major causative agent of chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HCV non-structural protein 5A (NS5A) is a dimeric phosphoprotein with a hyperphosphorylated form to act as a switch that regulates HCV replication and assembly. NS5A inhibitors have been utilized as the scaffold for combination therapy of direct-acting antiviral agents (dAA). However, the mode of action of NS5A inhibitors is still unclear due to the lack of mechanistic detail regarding NS5A phosphorylation and dimerization in the HCV life cycle. It has been demonstrated that phosphorylation of NS5A at Ser235 is essential for RNA replication of the JFH1 strain. In this report, we found that NS5A phosphomimetic Ser235 substitution (Ser-to-Asp mutation) formed a dimer that was resistant to disruption by NS5A inhibitors as was the NS5A resistance-associated substitution Y93H. phosphorylation of NS5A at Ser235 residue was required for the interaction of two NS5A-WT molecules in JFH1-based cell culture system but not absolutely required for dimerization of the NS5A-Y93H mutant. Interestingly, HCV nonstructural proteins from the subgenomic replicon NS3-5A was required for NS5A-WT dimerization but not required for NS5A-Y93H dimerization. Our data suggest that spontaneous Ser235 phosphorylation of NS5A and ensuing dimerization account for resistance of the JFH1/NS5A-Y93H mutant to NS5A inhibitors. © 2023 Elsevier Inc.

关键词： drug resistance Hepatitis C virus NS5A dimerization Ser235 phosphorylation

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