检索结果-内蒙古大学图书馆

作者： Lin, Yu-Chen Nguyen, Ha Ly Thi Balas, Valentina Emilia Lin, Tsung-Chih Kuo, I-Chun Department of Automatic Control Engineering Feng Chia University Taichung Taiwan Ph.D. Program in Electrical and Communications Engineering Feng Chia University Taichung Taiwan Automatics and Applied Software Department Aurel Vlaicu University of Arad ROU Romania Department of Electronic Engineering Feng-Chia University Taichung Taiwan Electronic Control Technology Group RD Division Automotive Research Testing Center Changhua Taiwan

This paper presents an adaptive prediction-based control scheme for developing an ecological cruise control (ECC) system when simultaneous running on the roads with the continuous curves and up-down slopes, which aims to guarantee the driving safety, riding comfort of a vehicle and enhance its fuel efficiency as well. This study is mainly divided into two parts: vehicle longitudinal speed control and vehicle lateral stability control. Firstly, based on GPS data, topographical information, and a fuel consumption model, the prediction based on approximate dynamic programming (AdP) using adaptive fuzzy neural networks (FNNs) is employed to calculate appropriate control signal required for ecological driving. Secondly, to maintain the vehicle stable on the curved road, the vehicle lateral stability control is presented utilizing sliding mode control approach. The effectiveness of the proposed ECC system, in view of stability, improved riding comfort and fuel efficiency, has been validated on the CarSim environment. © 2020 - IOS Press and the authors. All rights reserved.

关键词： Adaptive control systems

来源：评论

学校读者我要写书评

暂无评论

Targeting formyl peptide receptor 1 with anteiso-C13-surfactin for neutrophil-dominant acute respiratory distress syndrome

引用

British journal of pharmacology 2023年第16期180卷 2120-2139页

作者： Shun-Chin Yang Yi-Hsuan Wang Chiu-Ming Ho Yung-Fong Tsai Ping-Jyun Sung Tony Eight Lin Tsong-Long Hwang Department of Anesthesiology Taipei Veterans General Hospital and National Yang Ming Chiao Tung University Taipei 112 Taiwan. Graduate Institute of Natural Products and Graduate Institute of Biomedical Sciences College of Medicine Chang Gung University Taoyuan 333 Taiwan. Department of Anesthesiology Chang Gung Memorial Hospital Linkou Medical Center Taoyuan Taiwan. Graduate Institute of Clinical Medical Sciences College of Medicine Chang Gung University Taoyuan 333 Taiwan. National Museum of Marine Biology and Aquarium Pingtung 944 Taiwan. Graduate Institute of Cancer Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei 110 Taiwan. Ph.D. Program for Cancer Molecular Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei 110 Taiwan. Research Center for Chinese Herbal Medicine and Graduate Institute of Health Industry Technology College of Human Ecology Chang Gung University of Science and Technology 333 Taoyuan Taiwan. Healthy Aging Research Center Chang Gung University 333 Taoyuan Taiwan. Department of Chemical Engineering Ming Chi University of Technology New Taipei City 243 Taiwan.

BACKGROUNd ANd PURPOSE:Acute respiratory distress syndrome (ARdS) is a catastrophic pulmonary inflammatory dysfunction with a high mortality rate. An overwhelming immune response by neutrophils is a key feature in infective or sterile ARdS. The formyl peptide receptor 1 (FPR1) is a crucial damage-sensing receptor for inflammatory reactions in the initiation and progression of neutrophil-mediated ARdS. However, effective targets for controlling dysregulated neutrophilic inflammatory injuries in ARdS are limited. EXPERIMENTAL APPROACH:Human neutrophils were used to explore the anti-inflammatory effects of cyclic lipopeptide anteiso-C13-surfactin (IA-1) from marine Bacillus amyloliquefaciens. The lipopolysaccharide-induced model of ARdS in mice was used to determine the therapeutic potential of IA-1 in ARdS. Lung tissues were harvested for histology analyses. KEY RESULTS:The lipopeptide IA-1 inhibited immune responses of neutrophils, including respiratory burst, degranulation, and expression of adhesion molecules. IA-1 inhibited the binding of N-formyl peptides to FPR1 in human neutrophils and in hFPR1-transfected HEK293 cells. We identified IA-1 as a competitive FPR1 antagonist, thus diminishing the downstream signalling pathways involving calcium, mitogen-activated protein kinases and Akt. Furthermore, IA-1 ameliorated the inflammatory damage to lung tissue, by decreasing neutrophil infiltration, reducing elastase release and oxidative stress in endotoxemic mice. CONCLUSION ANd IMPLICATIONS:The lipopeptide IA-1 could serve as a therapeutic option for ARdS by inhibiting FPR1-mediated neutrophilic injury.

关键词： ARdS FPR1 N-formyl peptides lipopeptide marine Bacillus neutrophils

来源：评论

学校读者我要写书评

暂无评论

#41 : The Effectiveness of GnRH Agonist Administration Prior to Frozen-Thawed Embryo Transfer with Artificial Cycle: A Systematic Review and Meta-Analysis of different Protocols and Infertile Populations

引用

Fertility & Reproduction 2023年第4期5卷 337-337页

作者： Nguyen Tuong Ho dang Khanh Ngan Ho Xuan Hong Tomai Yu-Ming Hu Yen-Ping Ho Shyr-Yeu Lin Ruey-Sheng Wang Yi-Xuan Lee Shun-Jen Tan Shu-Huei Kao Chii-Ruey Tzeng International Ph.D. Program in Medicine College of Medicine Taipei Medical University Taipei Taiwan Taipei Fertility Center Taipei Taiwan School of Nutrition and Health Sciences College of Nutrition Taipei Medical University Taipei Taiwan Office of International Relations Ho Chi Minh City University of Medicine and Pharmacy Ho Chi Minh City Vietnam School of Medical Laboratory Science and Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan Ph.D. Program in Medical Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan

Background and Aims: We investigated the use of GnRHa pretreatment with different protocols and durations of treatment on pregnancy outcomes in women undergoing FET with artificial cycles (AC-FET). Method: A systematic search of PubMed, EMBASE, Google Scholar, and Cochrane library was performed to include only eligible peer-reviewed studies in English published before September 01, 2022. This review was registered on PROSPERO (CRd42022299259) Results: A total of 41 studies (43021 individuals) were included with low to moderate risk of bias. Overall, GnRHa pretreatment improved clinical pregnancy rate (CPR) (OR=1.27, 95%CI:1.12-1.44), implantation rate (IR) (OR=1.24, 95%CI:1.07-1.45), live birth rate (LBR) (OR=1.31, 95%CI:1.07-1.60) and endometrial thickness (Md=0.16, 95%CI:0.03-0.30). Subgroup analyses of randomized and non-randomized studies both demonstrated the benefits of GnRHa pretreatment in CPR, IR, and LBR. Protocols using depot GnRHa (OR=1.19, 95%CI:1.04-1.36) showed a better LBR than daily GnRHa (OR=1.04, 95%CI:0.93-1.16). Women administered GnRHa for more than one cycle had better CPR (OR=2.01, 95%CI:1.29-3.13) and IR (OR=2.14, 95%CI:0.98-4.67) than those treated with GnRHa within one cycle before FET ([OR=1.18, 95%CI:1.04-1.33] and [OR=1.16, 95%CI:1.01-1.32], respectively). In women with PCOS, pituitary suppression with GnRHa significantly improved CPR (OR=1.24, 95%CI:0.98-4.67), LBR (OR=1.22, 95%CI:1.05-1.42) and reduced the miscarriage rate (OR=0.75, 95%CI:0.59-0.95). In contrast, women with regular menstruation had a higher endometrial thickness (Md=0.16, 95%CI: 0.04-0.28) but no significant improvements in pregnancy outcomes. GnRHa pretreatment considerably improved IR (OR=2.21, 95%CI:1.60-3.07) in women with adenomyosis and CPR (OR=1.74, 95%CI:1.12-2.71), and LBR (OR=1.67, 95%CI:1.06-2.63) in women with endometriosis. Conclusion: GnRHa pretreatment, especially with longer suppression duration and the use of depot GnRHa, demonstrates a beneficial rol

关键词：

来源：评论

学校读者我要写书评

暂无评论

Corrigendum to "development and functional evaluation of a hyaluronic acid coated nano-formulation with kaempferol as a novel intra-articular agent for knee osteoarthritis treatment"[ Biomed. pharmacother. 175(2024) 116717]

引用

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024年 177卷 116944页

作者： Ching-Yu Lee Yu-Chu Chang Kai-Chiang Yang Yung-Fang Lin Alexander T H Wu Ching-Li Tseng International Ph.D. Program in Cell Therapy and Regenerative Medicine College of Medicine Taipei Medical University Taipei 110301 Taiwan Department of Orthopedics Taipei Medical University Hospital Taipei 110301 Taiwan Department of Orthopaedics School of Medicine College of Medicine Taipei Medical University Taipei 110301 Taiwan Orthopedic Research Center Taipei Medical University Hospital Taipei 110301 Taiwan. Department of Biochemistry and Molecular Cell Biology School of Medicine College of Medicine Taipei Medical University Taipei 110301 Taiwan. School of Dental Technology College of Oral Medicine Taipei Medical University Taipei 110301 Taiwan. Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei 110301 Taiwan International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei 110301 Taiwan. The Ph.D. Program for Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei 110301 Taiwan Clinical Research Center Taipei Medical University Hospital Taipei Medical University Taipei 110301 Taiwan. Electronic address: chaw1211@tmu.edu.tw. International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei 110301 Taiwan. Electronic address: chingli@tmu.edu.tw.

来源：评论

学校读者我要写书评

暂无评论

development of anti-aflatoxin B1 nanobodies from a novel mutagenesis-derived synthetic library for traditional Chinese medicine and foods safety testing

引用

Journal of biological engineering 2023年第1期17卷 30页

作者： Yu-Ching Lee Gar-Hwa Lai Tsai-Yu Lin Tien-Sheng Tseng Tsung-Hsun Tsai Wang-Chuan Chen Cheng-Chung Lee Keng-Chang Tsai TMU Research Center of Cancer Translational Medicine Taipei Medical University Taipei Taiwan. Ph.D. Program for Cancer Molecular Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei Taiwan. Ph.D. Program in Drug Discovery and Development Industry College of Pharmacy Taipei Medical University Taipei Taiwan. Ph.D. Program in Medical Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan. Department of Orthopedics Kaohsiung Veterans General Hospital Kaohsiung Taiwan. Institute of Molecular Biology National Chung Hsing University Taichung Taiwan. Department of Psychiatry Kaohsiung Armed Forces General Hospital Kaohsiung Taiwan. The School of Chinese Medicine for Post Baccalaureate I-Shou University Kaohsiung Taiwan. Department of Chinese Medicine E-Da Hospital Kaohsiung Taiwan. The Ph.D. Program for Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei Taiwan. Ph.D. Program in Medical Biotechnology College of Medical Science and Technology Taipei Medical University Taipei Taiwan. tkc@nricm.edu.tw. National Research Institute of Chinese Medicine Ministry of Health and Welfare No. 155-1 Sec. 2 Linong St. Beitou District Taipei 11221 Taiwan. tkc@nricm.edu.tw.

BACKGROUNd:The main commercially available methods for detecting small molecules of mycotoxins in traditional Chinese medicine (TCM) and functional foods are enzyme-linked immunosorbent assay and mass spectrometry. Regarding the development of diagnostic antibody reagents, effective methods for the rapid preparation of specific monoclonal antibodies are inadequate. METHOdS:In this study, a novel synthetic phage-displayed nanobody Golden Glove (SynaGG) library with a glove-like cavity configuration was established using phage display technology in synthetic biology. We applied this unique SynaGG library on the small molecule aflatoxin B1 (AFB1), which has strong hepatotoxicity, to isolate specific nanobodies with high affinity for AFB1. RESULT:These nanobodies exhibit no cross-reactivity with the hapten methotrexate, which is recognized by the original antibody template. By binding to AFB1, two nanobodies can neutralize AFB1-induced hepatocyte growth inhibition. Using molecular docking, we found that the unique non-hypervariable complementarity-determining region 4 (CdR4) loop region of the nanobody was involved in the interaction with AFB1. Specifically, the CdR4's positively charged amino acid arginine directed the binding interaction between the nanobody and AFB1. We then rationally optimized the interaction between AFB1 and the nanobody by mutating serine at position 2 into valine. The binding affinity of the nanobody to AFB1 was effectively improved, and this result supported the use of molecular structure simulation for antibody optimization. CONCLUSION:In summary, this study revealed that the novel SynaGG library, which was constructed through computer-aided design, can be used to isolate nanobodies that specifically bind to small molecules. The results of this study could facilitate the development of nanobody materials to detect small molecules for the rapid screening of TCM materials and foods in the future.

关键词： Aflatoxin B1 Glove-like cavity Nanobody Nonhypervariable complementarity-determining region 4 loop phage display technology

来源：评论

学校读者我要写书评

暂无评论

discovery of dual-specificity tyrosine-phosphorylation-regulated kinase 1A (dYRK1A) inhibitors using an artificial intelligence model and their effects on tau and tubulin dynamics

引用

Biomedicine and pharmacotherapy 2024年 181卷 117688-117688页

作者： Chen, Jun-Hong Tu, Huang-Ju Lin, Tony Eight Peng, Zhao-Xiang Wu, Yi-Wen Yen, Shih-Chung Sung, Tzu-Ying Hsieh, Jui-Hua Lee, Hsueh-Yun Pan, Shiow-Lin HuangFu, Wei-Chun Hsu, Kai-Cheng Graduate Institute of Cancer Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei Taiwan Ph.D. Program for Cancer Molecular Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei Taiwan Warshel Institute for Computational Biology The Chinese University of Hong Kong Guangdong Shenzhen China Division of Translational Toxicology National Institute of Environmental Health Sciences National Institutes of Health Durham NC United States School of Pharmacy College of Pharmacy Taipei Medical University Taipei Taiwan TMU Research Center of Cancer Translational Medicine Taipei Medical University Taipei Taiwan Cancer Center Wan Fang Hospital Taipei Medical University Taipei Taiwan

The dual-specificity tyrosine-phosphorylation-regulated kinase 1 A (dYRK1A) presents a promising therapeutic target for neurological diseases. However, current inhibitors lack selectivity, which can lead to unexpected side effects and increase the difficulty of studying dYRK1A. Therefore, identifying selective inhibitors targeting dYRK1A is essential for reducing side effects and facilitating neurological disease research. This study aimed to discover dYRK1A inhibitors through a screening pipeline incorporating a deep neural network (dNN) model. Herein, we report an optimized model with an accuracy of 0.93 on a testing set. The pipeline was then performed to identify potential dYRK1A inhibitors from the National Cancer Institute (NCI) library. Four novel dYRK1A inhibitors were identified, and compounds NSC657702 and NSC31059 were noteworthy for their potent inhibition, with IC₅₀ values of 50.9 and 39.5 nM, respectively. NSC31059 exhibited exceptional selectivity across 70 kinases. The compounds also significantly reduced dYRK1A-induced tau phosphorylation at key sites associated with the pathology of neurodegenerative diseases. Moreover, they promoted tubulin polymerization, suggesting a role in microtubule stabilization. Cytotoxicity assessments further confirmed the neuronal safety of the compounds. Together, the results demonstrated a promising screening pipeline and novel dYRK1A inhibitors as candidates for further optimization and development. © 2024 The Authors

关键词： deep Learning dYRK1A Tau

来源：评论

学校读者我要写书评

暂无评论

COVId-19 Associated Bacteremia withChryseobacterium indologenesCo-HarboringblaINd-2,blaCIAandblaCcrA

引用

Infection and drug Resistance 2022年 15卷 167-170页

作者： Ting-Kuang Yeh Zong-Hao Li Yao-Ting Huang Po-Yu Liu 1 Division of Infectious Diseases Department of Internal Medicine Taichung Veterans General Hospital Taichung Taiwan 2 Department of Computer Science and Information Engineering National Chung Cheng University Chiayi Taiwan 1 Division of Infectious Diseases Department of Internal Medicine Taichung Veterans General Hospital Taichung Taiwan3 Ph.D. Program in Translational Medicine National Chung Hsing University Taichung Taiwan4 Rong Hsing Research Center for Translational Medicine National Chung Hsing University Taichung Taiwan

We report a COVId-19 case with carbapenem resistantChryseobacterium indologenesbacteremia. Whole genome sequencing identified the presence ofblaINd-2,blaCIAandblaCcrA. To our knowledge, this is the first report ofChryseobacterium indologenescomplicating COVId-19 and the detection ofblaCcrAinC. indologenes. The presence ofblaCcrAinChryseobacteriumwas overlooked previously may related to substantial sequence divergence with the original allele inBacteroides fragilis. Antimicrobial resistance (AMR) is a challenge to global health in the age of COVId-19 pandemic. Further study and surveillance of underlying mechanisms is needed.

关键词： COVId-19 SARS-CoV-2 Chryseobacterium indologenes resistance

来源：评论

学校读者我要写书评

暂无评论

Ohwia caudata extract relieves the IL-17A-induced inflammatory response of synoviocytes through modulation of SOCS3 and JAK2/STAT3 activation

引用

Environmental Toxicology 2023年第8期38卷 1914-1924页

作者： Lu, Cheng-You Kuo, Chia-Hua Kuo, Wei-Wen Hsieh, dennis Jine-Yuan Wang, Tso-Fu Shih, Cheng-Yen Lin, Pi-Yu Lin, Shinn-Zong Ho, Tsung-Jung Huang, Chih-Yang Department of Post-Baccalaureate Medicine College of Medicine National Chung Hsing University Taichung Taiwan Department of Sports Sciences University of Taipei Taipei Taiwan Department of Biological Science and Technology China Medical University Taichung Taiwan Ph.D. Program for Biotechnology Industry China Medical University Taichung Taiwan School of Medical Laboratory and Biotechnology Chung Shan Medical University Taichung Taiwan Clinical Laboratory Chung Shan Medical University Hospital Taichung Taiwan Department of Hematology and Oncology Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Department of Medicine Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Tzu Chi University Hualien Taiwan Buddhist Tzu Chi Medical Foundation Hualien Taiwan Buddhist Compassion Relief Tzu Chi Foundation Hualien Taiwan Bioinnovation Center Buddhist Tzu Chi Medical Foundation Hualien Taiwan Department of Neurosurgery Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Integration of Chinese Medicine and Modern Medicine Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Department of Chinese Medicine Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan Department of Biological Science and Technology Asia University Taichung Taiwan Center of General Education Buddhist Tzu Chi Medical Foundation Tzu Chi University of Science and Technology Hualien Taiwan

Fibroblast-like synoviocytes accumulation, proliferation and activation, and the subsequent inflammatory mediators production play a key role in the progression of rheumatoid arthritis (RA). It is well established that Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling triggers inflammation, and induces cytokine levels in RA. Ohwia caudata have long been used against many disorders. However, in RA, the effects of O. caudata have not been elucidated. In the current study, synoviocytes were used to evaluate the suppressive effects of O. caudate extract (OCE) on the pro-inflammatory cytokines production. In vitro, the underlying mechanisms by which OCE inhibits inflammatory response through regulation of suppressors of cytokine signaling 3 (SOCS3) and JAK2/STAT3 expression in IL-17A-treated HIG-82 synoviocytes were investigated. The results demonstrated that the proliferation of IL-17A-challenged cells were increased in comparison with non-stimulated control cells. The synoviocyte proliferation was decreased significantly of OCE concentrations in dose dependent manner. The p-JAK2, p-STAT3, interleukin (IL)-1β, and IL-6 were reduced in IL-17A-challenged cells treated with OCE. Furthermore, AZd1480 (a JAK2-specific inhibitor) or WP1066 (a STAT3-specific inhibitor) affected the inflammatory mediators production in IL-17A-challenged synoviocytes, and OCE failed to mitigate the IL-17A-induced inflammatory mediators and SOCS3, acting as a feedback inhibitor of the JAK/STAT3 pathway, in the presence of SOCS3 siRNA, indicating that the beneficial effects of OCE on the regulation of inflammatory response homeostasis were dependent on SOCS3 and the JAK2/STAT3 signaling pathway. Our study also showed that SOCS3 was markedly activated by OCE in RA fibroblast-like synoviocytes, thereby decreasing the JAK/STAT3 pathway, and the IL-1β, and IL-6 activation. Thus, O. caudate should be further investigated as a candidate anti-inflammatory and anti-a

关键词： Chemical activation

来源：评论

学校读者我要写书评

暂无评论

Friction stir welding of AZ31 magnesium alloy: A review

Friction stir welding of AZ31 magnesium alloy: A review

引用

2020 International Conference on Advances in design, Materials and Manufacturing

作者： desai, Ashish M. Khatri, Bharat C. Patel, Vivek Rana, Harikrishna Mechanical Engineering Department Government Polytechnic Himatnagar383001 India Mechanical Engineering Department Government Engineering College Modasa383315 India Department of Engineering Science University West Trollhättan46186 Sweden Mechanical Engineering Department School of Technology Pandit Deendayal Petroleum University Gandhinagar382007 India Mechanical Engineering Department LDRP Institute of Technology and Research Gandhinagar382015 India Ph.D. Research Scholar Gujarat Technological University Gujarat Ahmedabad382424 India

Friction Stir Welding (FSW) has been proven to be a unique and substantial development in welding technology for the last two decades. In recent times, the research and advancement in FSW and related knowledge have been emerging quickly worldwide. FSW has several benefits when welding lightweight alloys like magnesium alloys. AZ series of magnesium alloy is widely used in structural applications and have a great scope to cater to customized solutions for several industries like automotive, aerospace, railway, etc. In this review article, the basic principles of FSW along with recent developments in the field of FSW for AZ 31 magnesium alloys have been described in detail. Outlooks to foster the research are recognised. © 2020 Elsevier Ltd. All rights reserved.

关键词： Friction stir welding

来源：评论

学校读者我要写书评

暂无评论

The biocompatibility and hemostatic efficacy of silk fibroin nanofibrils fabricated by Shear-Induced phase separation process

引用

Chemical engineering Journal 2023年 474卷

作者： Lu, Po-Wen Wu, Yu-Tzu Chang, Liang-Yu Lu, Tzu-Wei Jaihao, Chonlachat Huang, Ching-Shuan Murphy Chuang, Mo-Fei Lin, Chunche Chen, Innie Brangule, Agnese Zarkov, Aleksej Kareiva, Aivaras Yang, Jen-Chang Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei11052 Taiwan Division of Gastroenterology and Hepatology Department of Internal Medicine Shuang Ho Hospital Taipei Medical University Zhongzheng Rd. Taipei Zhonghe23561 Taiwan Graduate Institute of Nanomedicine and Medical Engineering College of Biomedical Engineering Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Department of Dentistry Taipei Medical University Hospital Taipei11031 Taiwan Institute of Organic and Polymeric Materials National Taipei University of Technology Taipei106344 Taiwan Research and Development Center for Smart Textile Technology National Taipei University of Technology Taipei106344 Taiwan Innie Chen Department of Obstetrics and Gynecology University of Ottawa OttawaONK1H 7W9 Canada Department of Pharmaceutical Chemistry Riga Stradins University RīgaLV-1007 Latvia Institute of Chemistry Vilnius University Naugarduko 24 VilniusLT-03225 Lithuania International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Research Center of Biomedical Device Taipei Medical University Taipei11031 Taiwan Research Center of Digital Oral Science and Technology Taipei Medical University Taipei11031 Taiwan

The objectives of this study were to investigate the biocompatibility and hemostatic efficacy of silk fibroin nanofibrils (SFNFs). Unlike a conventional dialysis/freeze-drying process, SFNFs were prepared using a novel shear-induced phase-separation method. The morphology, particle size distribution, protein secondary structures, and crystalline structures of the resulting SFNFs were respectively investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), laser diffraction (Ld), Fourier-transform infrared (FTIR) spectroscopy, and x-ray diffraction (XRd) deconvolution analyses. Polar and non-polar extractants of SFNFs under a bath ratio of 0.2 g/mL were collected after individually soaking them into 0.9% normal saline and cottonseed oil. In vitro hemolysis ratios, biocompatibility, and sensitization tests were carried out. Then, the hemostatic efficacy was assayed using a rat liver punch biopsy model. Compared to dialyzed/freeze-dried SF, the harvested SFNFs revealed high surface area in morphology. The median particle size distribution, d50, was 294 μm for SFNFs. Shear-induced hydrogen bonding, crystallization, and phase separation tended to give the SFNFs a high crystalline index (CI) and degree of crystallinity (dC). The hemolysis ratio of SFNFs was 0.86 ± 0.40%, and it was classified as being non-hemolytic (0%∼2%). The test results of toxicity and skin sensitization were both negative. The hemostasis time for SFNFs (2.8 ± 0.6 min) was statistically less than that of oxidized cellulose (4.3 ± 0.9 min) (p © 2023 Elsevier B.V.

关键词： Biocompatibility

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：