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Corrigendum to "Ultrafast absorption mechanism of oil-emulsified micelles onto ferrous absorbents with dielectrophoresis force in the presence of polarization" [J Hazard Mater 460 (2023) 132436]

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Journal of hazardous materials 2024年 464卷 132601页

作者： Chun-Yao Huang Feng-Yen Lin Chien-Hsing Lu Jem-Kun Chen Department of Materials Science and Engineering National Taiwan University of Science and Technology 43 Section 4 Keelung Road Taipei 106 Taiwan ROC Taipei Heart Institute Taipei Medical University 250 Wu-Hsing Street Taipei 110 Taiwan ROC Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital 252 Wu-Hsing Street Taipei 110 Taiwan ROC Department of Internal Medicine School of Medicine College of Medicine Taipei Medical University 250 Wu-Hsing Street Taipei 110 Taiwan ROC Department of Biomedical Sciences and Engineering National Central University 300 Zhongda Road Taoyuan City 320317 Taiwan ROC. Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital 252 Wu-Hsing Street Taipei 110 Taiwan ROC. Department of Obstetrics and Gynecology Taichung Veterans General Hospital 1650 Taiwan Boulevard Sect. 4 Taichung 40705 Taiwan ROC Ph.D. Program in Translational Medicine and Rong Hsing Research Center for Translational Medicine National Chung Hsing University Taichung Taiwan ROC. Taipei Heart Institute Taipei Medical University 250 Wu-Hsing Street Taipei 110 Taiwan ROC. Electronic address: jkchen@mail.ntust.edu.tw.

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Melatonin Enhanced Microglia M2 Polarization in Rat Model of Neuro-inflammation Via Regulating ER Stress/PPARδ/SIRT1 Signaling Axis

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Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune pharmacology 2024年第1期19卷 11页

作者： Hung-Chuan Pan Cheng-Ning Yang Wen-Jane Lee Jason Sheehan Sheng-Mao Wu Hong-Shiu Chen Mao-Hsun Lin Li-Wei Shen Shu-Hua Lee Chin-Chang Shen Liang-Yi Pan Shing-Hwa Liu Meei-Ling Sheu Neurological Institute Taichung Veterans General Hospital Taichung Taiwan. Department of Medical Research Taichung Veterans General Hospital Taichung Taiwan. Ph.D. Program in Translational Medicine Rong Hsing Research Center for Translational Medicine National Chung Hsing University Taichung Taiwan. Doctoral Program in Biotechnology Industrial Management and Innovation National Chung Hsing University Taichung Taiwan. College of Medicine and Life Science Chung Hwa University of Medical Technology Tainan Taiwan. Department of Dentistry School of Dentistry College of Medicine National Taiwan University Taipei Taiwan. Department of Neurosurgery University of Virginia Charlottesville VA USA. Institute of Biomedical Sciences National Chung Hsing University Taichung Taiwan. Department of Neurosurgery Tungs' Taichung MetroHarbor Hospital Taichung Taiwan. Division of Neurology Department of Internal Medicine Ditmanson Medical Foundation Chia-Yi Christian Hospital Chia-Yi Taiwan. Institute of Nuclear Energy Research Atomic Energy Council Taoyuan Taiwan. School of Medicine Kaohsiung Medical University Taichung Taiwan. Institute of Toxicology College of Medicine National Taiwan University Taipei 100 Taiwan. Department of Medical Research Taichung Veterans General Hospital Taichung Taiwan. mlsheu@nchu.edu.tw. Ph.D. Program in Translational Medicine Rong Hsing Research Center for Translational Medicine National Chung Hsing University Taichung Taiwan. mlsheu@nchu.edu.tw. Institute of Biomedical Sciences National Chung Hsing University Taichung Taiwan. mlsheu@nchu.edu.tw. Institute of Biomedical Sciences College of Life Sciences National Chung Hsing University 250 Kuo Kuang Road Taichung 402 Taiwan. mlsheu@nchu.edu.tw.

Neuro-inflammation involves distinct alterations of microglial phenotypes, containing nocuous pro-inflammatory M1-phenotype and neuroprotective anti-inflammatory M-phenotype. Currently, there is no effective treatment for modulating such alterations. M1/M2 marker of primary microglia influenced by Melatonin were detected via qPCR. Functional activities were explored by western blotting, luciferase activity, EMSA, and ChIP assay. Structure interaction was assessed by molecular docking and LIGPLOT analysis. ER-stress detection was examined by ultrastructure TEM, calapin activity, and ERSE assay. The functional neurobehavioral evaluations were used for investigation of Melatonin on the neuroinflammation in vivo. Melatonin had targeted on Peroxisome Proliferator Activated Receptor delta (PPARδ) activity, boosted LPS-stimulated alterations in polarization from the M1 to the M2 phenotype, and thereby inhibited NFκB-IKKβ activation in primary microglia. The PPARδ agonist L-165,041 or over-expression of PPARδ plasmid (ov-PPARδ) showed similar results. Molecular docking screening, dynamic simulation approaches, and biological studies of Melatonin showed that the activated site was located at PPARδ (phospho-Thr256-PPARδ). Activated microglia had lowered PPARδ activity as well as the downstream SIRT1 formation via enhancing ER-stress. Melatonin, PPARδ agonist and ov-PPARδ all effectively reversed the above-mentioned effects. Melatonin blocked ER-stress by regulating calapin activity and expression in LPS-activated microglia. Additionally, Melatonin or L-165,041 ameliorated the neurobehavioral deficits in LPS-aggravated neuroinflammatory mice through blocking microglia activities, and also promoted phenotype changes to M2-predominant microglia. Melatonin suppressed neuro-inflammation in vitro and in vivo by tuning microglial activation through the ER-stress-dependent PPARδ/SIRT1 signaling cascade. This treatment strategy is an encouraging pharmacological approach for the remedy

关键词： Melatonin Microglia, Neuro-inflammation PPARδ SIRT1

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Unlocking the potential of bacterial cellulose: synthesis, functionalization, and industrial impact

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International Journal of Biological Macromolecules 2025年第PT 3期311卷 143951页

作者： Kumaravel, Ashokkumar Lin, Shin-Ping Santoso, Shella Permatasari Shanmugasundaram, Saranya Hsu, Hsien-Yi Hsieh, Chang-Wei Chou, Yu-Chieh Lin, Hui-Wen Cheng, Kuan-Chen Institute of Food Science and Technology National Taiwan University Daan District Taipei10617 Taiwan School of Food Safety Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Ph.D. Program in Drug Discovery and Development Industry Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Chemical Engineering Master Program Widya Mandala Surabaya Catholic University Kalijudan 37 Surabaya60114 Indonesia Collaborative Research Center for Zero Waste and Sustainability Widya Mandala Surabaya Catholic University Kalijudan 37 Surabaya60114 Indonesia Department of Chemical Engineering Faculty of Engineering Widya Mandala Catholic University Surabaya Kalijudan 37 Surabaya60114 Indonesia Department of Chemical Engineering University of Ulsan 93 Daehak-ro Nam-gu Ulsan44610 Korea Republic of City University of Hong Kong Kowloon Tong Hong Kong Shenzhen Research Institute of City University of Hong Kong Shenzhen518057 China Department of Food Science and Biotechnology National Chung-Hsing University Taichung City40227 Taiwan Department of Food Science National Ilan University Yilan City260007 Taiwan Institute of Medicine Chung Shan Medical University Taichung40201 Taiwan Department of Medical Research Chung Shan Medical University Hospital Taichung40201 Taiwan Institute of Biotechnology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei10607 Taiwan Department of Optometry Asia University 500 Lioufeng Rd. Wufeng Taichung41350 Taiwan Department of Medical Research China Medical University Hospital China Medical University 91 Hsueh-Shih Road Taichung40402 Taiwan Department of Food Science Fu Jen Catholic University 510 Zhongzheng Rd. Xinzhuang Dist. New Taipei City242062 Taiwan

In recent years, bacterial cellulose (BC), a crystalline and nanoscale fibrillar polymer, has garnered significant interest due to its superior physical, chemical, and mechanical properties compared to plant cellulose. Inherent features of BC, which include high biodegradability, mechanical strength, and biocompatibility, make it a suitable material for use in a wide variety of applications, particularly in the domains of biomedicine and environmental science. However, realizing its full potential requires targeted chemical or physical modifications. Recent developments have focused on in-situ and ex-situ BC modification for various applications. Incorporating functional compounds into cellulose during BC synthesis enhances its strength and functional properties. Additionally, the material properties of BC, such as water content, thermal stability, and antibacterial capabilities, can be enhanced through post-synthesis modifications, including chemical acetylation, phosphorylation, and integration with other biopolymers. Currently, the applicability of BC is being improved using new modification techniques, which involve genetically engineered strains to enhance polymer characteristics and market potential. The review highlights recent advancements in BC research and novel modification strategies that enhance its applications in advanced biomedical engineering and sustainable packaging. © 2025

关键词： Acetylation

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Narciclasine suppresses oral cancer metastasis by modulating cathepsin B and extracellular signal-related kinase pathways

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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023年 158卷 114159页

作者： Mu-Kuei Shieu Hsin-Yu Ho Chia-Chieh Lin Yu-Sheng Lo Yi-Ching Chuang Ming-Ju Hsieh Mu-Kuan Chen Division of General Practice Department of Medical Education Changhua Christian Hospital Changhua 500 Taiwan. Oral Cancer Research Center Changhua Christian Hospital Changhua 500 Taiwan. Oral Cancer Research Center Changhua Christian Hospital Changhua 500 Taiwan Ph.D. Program in Tissue Engineering and Regenerative Medicine College of Medicine National Chung Hsing University Taichung 402 Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung 404 Taiwan. Electronic address: 170780@cch.org.tw. Department of Otorhinolaryngology Head and Neck Surgery Changhua Christian Hospital Changhua 500 Taiwan. Electronic address: 53780@cch.org.tw.

Oral cancer is a malignancy with unfavorable prognosis due to its high rates of recurrence and lymph node metastasis. Narciclasine is extracted from Narcissus species (Amaryllidaceae), which have antitumor and anti-inflammatory properties. However, the antitumor properties of narciclasine toward oral cancer remain unclear. The present study explored the antimetastatic effects of narciclasine in oral cancer as well as the underlying molecular mechanisms. We treated three oral cancer cell lines with noncytotoxic concentrations of narciclasine and discovered a dose-dependent antimetastatic effect. Mitogen-activated protein kinase (MAPK) pathways, including extracellular signal-related kinase (ERK), p38, and c-Jun N-terminal kinase (JNK), were regulated by narciclasine. We further discovered the ERK pathway to directly affect narciclasine-induced metastasis inhibition by combining treatment with narciclasine and ERK inhibitor. Furthermore, downregulation of cathepsin B (CTSB) in the SAS and SCC-47 cell lines revealed the critical role of CTSB in the antimetastatic effect of narciclasine. Our findings indicate that narciclasine inhibits oral cancer metastasis by regulating the ERK pathway and CTSB. This study provides evidence of the mechanism of narciclasine-induced inhibition oral cancer metastasis and suggests potential targets for use in oral cancer treatment.

关键词： Cathepsin B Metastasis Narciclasine Oral cancer

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SENP2 restrains the generation of pathogenic Th17 cells in mouse models of colitis

Communications biology

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Communications biology 2023年第1期6卷 629页

作者： Tsan-Tzu Yang Ming-Feng Chiang Che-Chang Chang Shii-Yi Yang Shih-Wen Huang Nan-Shih Liao Hsiu-Ming Shih Wei Hsu Kuo-I Lin Graduate Institute of Immunology College of Medicine National Taiwan University Taipei 10002 Taiwan. Genomics Research Center Academia Sinica Taipei 11529 Taiwan. The Ph.D. Program for Translational Medicine College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan. Institute of Molecular Biology Academia Sinica Taipei 11529 Taiwan. Institute of Biomedical Sciences Academia Sinica Taipei 11529 Taiwan. Forsyth Institute Harvard School of Dental Medicine Harvard Stem Cell Institute Harvard University Cambridge MA 02142 USA. Graduate Institute of Immunology College of Medicine National Taiwan University Taipei 10002 Taiwan. kuoilin@gate.sinica.edu.tw. Genomics Research Center Academia Sinica Taipei 11529 Taiwan. kuoilin@gate.sinica.edu.tw.

The molecular mechanisms contributing to the regulation of Th17-mediated inflammation remain underexplored. We here report a SUMO-specific protease (SENP)2-mediated pathway induced in pathogenic Th17 cells that restricts the pathogenesis of inflammatory colitis. SENP2 regulates the maturation of small ubiquitin-like modifiers (SUMO) and recycles SUMO from the substrate proteins. We find higher levels of SENP2 in pathogenic Th17 cells. By deleting Senp2 in T-cell lineages in mice, we demonstrate that the lack of Senp2 exacerbates the severity of experimental colitis, which is linked to elevated levels of GM-CSFIL-17A pathogenic Th17 cells and more severe dysbiosis of the intestinal microbiome. Adoptive transfer experiments demonstrate the cell-autonomous effect of Senp2 in restraining Th17 differentiation and colitis. The enzymatic activity of SENP2 is important for deSUMOylation of Smad4, which reduces Smad4 nuclear entry and Rorc expression. Our findings reveal a SENP2-mediated regulatory axis in the pathogenicity of Th17 cells.

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Self-assemble nano particle array on transparent glass as selective absorption spectra (SANP-GTAS) 22

Self-assemble nano particle array on transparent glass as se...

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22nd International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2018

作者： Koduri, Manohar Prasad Kumar, Ashish Goudar, Venkanagouda Tseng, Fan Gang International Intercollegiate Ph.D. Program National Tsing Hua University Taiwan Department of Engineering and System Science Frontier Research Center on Fundamental and Applied Sciences of Matters National Tsing Hua University Taiwan Research Center for Applied Sciences Academia Sinica Taipei Taiwan

ISBN: (纸本)9781510897571

In this study, we demonstrated a novel technique in selective absorption spectra (AS), on a transparent glass (GT) formed by self-assembled Nanoparticle array (SANP) and observed a narrow band absorption in the green wavelength region. The single layer deposition on a transparent glass is achieved by using the in-house developed self-assembly deposition technique. The monolayer retains the shape of hexagonal arrangement in between Nanoparticles and forms a regular array structure. Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

关键词： Self assembly

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Constructing a Smart Home Control System with the Internet of Things

Constructing a Smart Home Control System with the Internet o...

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IEEE Asia-Pacific Conference on Antennas and Propagation (APCAP)

作者： Huan-Po Hsu Kun-Ming Yu Wen Ouyang Cheng Jie Xu Ph.D. Program in Engineering Science Chung Hua University Hsinchu Taiwan Chung-Hua University Hsinchu Taiwan Faculty of Computer and Software Engineering Huaiyin Institute of Technology Jiangsu China

The development of Internet of Things (IoT) makes the application of smart homes grow rapidly. It is very popular to install smart appliances in the house. However, building a smart control system at home not only costs a lot but also has many limitations. For this reason, this study proposes a smart homes control system to easily integrate IoT, WSN, smart robot and single-board computer to implement smart home applications. We use wireless technology and automatic equipment to avoid excessive communication cable and to make the house more intelligent to keep in-house movement unimpeded and indoor space tidy and to let appliance adjust appropriate environment settings automatically. This system brings intelligence and convenience to home, making living environment more comfortable.

关键词： Robot sensing systems Smart homes Control systems Robot kinematics Wireless sensor networks Internet of Things

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Associations of COVId-19-related fear with kidney disease quality of life and its subscales among hemodialysis patients as modified by health literacy: a multi-hospital survey

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Health Psychology and Behavioral Medicine 2024年第1期12卷 2376585页

作者： pham, Minh d. Tran, Tu T. duong, Tuyen Van do, Binh N. dang, Loan T. Nguyen, dung H. Hoang, Trung A. Nguyen, Hoang C. Le, Lan T. H. pham, Linh V. Nguyen, Lien T. H. Nguyen, Hoi T. Trieu, Nga T. do, Thinh V. Trinh, Manh V. Ha, Tung H. phan, dung T. Nguyen, Thao T. P. Nguyen, Kien T. Department of Nutrition Military Hospital 103 Department of Nutrition Vietnam Military Medical University International Ph.D. Program in Medicine College of Medicine Taipei Medical University Department of Internal Medicine Thai Nguyen University of Medicine and Pharmacy Department of Nephro-Urology and Dialysis Thai Nguyen National Hospital School of Nutrition and Health Sciences Taipei Medical University Department of Military Science Vietnam Military Medical University Department of Infectious Diseases Vietnam Military Medical University Faculty of Nursing and Midwifery Hanoi Medical University School of Nursing National Taipei University of Nursing and Health Sciences Thoracic and Vascular surgery department Bach Mai Hospital Hemodialysis Department Nephro-Urology-Dialysis Center Bach Mai Hospital Director Office Thai Nguyen National Hospital President Office Thai Nguyen University of Medicine and Pharmacy Training and Direction of Healthcare Activity Center Thai Nguyen National Hospital Biochemistry Department Thai Nguyen National Hospital Department of Pulmonary & Cardiovascular Diseases Hai Phong University of Medicine and Pharmacy Hospital President Office Hai Phong University of Medicine and Pharmacy Director Office Hai Phong International Hospital Hemodialysis Division Hai Phong International Hospital Director Office Bai Chay Hospital Director Office Quang Ninh General Hospital Director Office General Hospital of Agricultural Faculty of Nursing Hanoi University of Business and Technology Nursing Office Thien An Obstetrics and Gynecology Hospital Institute for Community Health Research University of Medicine and Pharmacy Hue University Department of Health Promotion Faculty of Social and Behavioral Sciences Hanoi University of Public Health

Background: Receiving hemodialysis treatment makes end-stage renal disease (ESRd) patients highly vulnerable amidst the COVId-19 pandemic. Hence, their kidney disease quality of life (KdQOL) is affected. We aimed to examine the association between fear of COVId-19 (FCoV-19) and KdQOL, and the effect modification of Health literacy (HL) on this association. Material and Methods: A survey was conducted at 8 hospitals from July 2020 to March 2021 on 972 patients. data collection includes socio-demographic factors, clinical parameters, HL, digital healthy diet literacy (ddL), hemodialysis diet knowledge (HdK), FCoV-19, suspected COVId-19 symptoms (S-COVId-19-S), and KdQOL. Results: Higher HL scores B = 0.13 (95% CI = 0.06–0.21, p = 0.001) and HdK scores B = 0.58 (95% CI = 0.31–0.85, p = 0.001) were associated with higher KdQOL scores. Whereas, S-COVId-19-S B = −6.12 (95% CI = −7.66 to–4.58, p = 0.001) and FCoV-19 B = −0.91 (95% CI = −1.03 to–0.80, p = 0.001) were associated with lower KdQOL scores. Notably, higher HL scores significantly attenuate the negative impact of FCoV-19 on overall KdQOL and the kidney disease component summary. Conclusions: In hemodialysis patients, FCoV-19 and S-COVId-19-S were associated with a lower KdQOL. Health literacy significantly mitigates the negative impact of FCoV-19 on KdQOL. Strategic public health interventions to improve HL are suggested to protect patient’s KdQOL during the pandemic. © 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

关键词： Fear of COVId-19 health literacy kidney disease component summary mental component summary physical component summary

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Suppression of the human malic enzyme 2 modifies energy metabolism and inhibits cellular respiration

Communications biology

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Communications biology 2023年第1期6卷 548页

作者： Ju-Yi Hsieh Kun-Chi Chen Chun-Hsiung Wang Guang-Yaw Liu Jie-An Ye Yu-Tung Chou Yi-Chun Lin Cheng-Jhe Lyu Rui-Ying Chang Yi-Liang Liu Yen-Hsien Li Mau-Rong Lee Meng-Chiao Ho Hui-Chih Hung Department of Life Sciences National Chung Hsing University Taichung 402 Taiwan ROC. Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University Taichung 402 Taiwan ROC. Institute of Biological Chemistry Academia Sinica Taipei 115 Taiwan ROC. Institute of Medicine College of Medicine Chung Shan Medical University Taichung 402 Taiwan ROC. Instrument Center Office of Research and Development National Chung Hsing University Taichung 40227 Taiwan ROC. Department of Chemistry National Chung Hsing University Taichung 402 Taiwan ROC. Institute of Biological Chemistry Academia Sinica Taipei 115 Taiwan ROC. joeho@gate.sinica.edu.tw. Institute of Biochemical Sciences National Taiwan University Taipei 106 Taiwan ROC. joeho@gate.sinica.edu.tw. Department of Life Sciences National Chung Hsing University Taichung 402 Taiwan ROC. hchung@dragon.nchu.edu.tw. Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University Taichung 402 Taiwan ROC. hchung@dragon.nchu.edu.tw. Institute of Genomics and Bioinformatics National Chung Hsing University Taichung 402 Taiwan ROC. hchung@dragon.nchu.edu.tw. Advanced Plant and Food Crop Biotechnology Center National Chung Hsing University Taichung 402 Taiwan ROC. hchung@dragon.nchu.edu.tw.

Human mitochondrial NAd(P)-dependent malic enzyme (ME2) is well-known for its role in cell metabolism, which may be involved in cancer or epilepsy. We present potent ME2 inhibitors based on cyro-EM structures that target ME2 enzyme activity. Two structures of ME2-inhibitor complexes demonstrate that 5,5'-Methylenedisalicylic acid (MdSA) and embonic acid (EA) bind allosterically to ME2's fumarate-binding site. Mutagenesis studies demonstrate that Asn35 and the Gln64-Tyr562 network are required for both inhibitors' binding. ME2 overexpression increases pyruvate and NAdH production while decreasing the cell's NAd/NAdH ratio; however, ME2 knockdown has the opposite effect. MdSA and EA inhibit pyruvate synthesis and thus increase the NAd/NAdH ratio, implying that these two inhibitors interfere with metabolic changes by inhibiting cellular ME2 activity. ME2 silence or inhibiting ME2 activity with MdSA or EA decreases cellular respiration and ATP synthesis. Our findings suggest that ME2 is crucial for mitochondrial pyruvate and energy metabolism, as well as cellular respiration, and that ME2 inhibitors could be useful in the treatment of cancer or other diseases that involve these processes.

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Building a Model for Predicting Target Gene Expression in Rice T-dNA Insertional Mutants by Machine Learning Approaches

Research Square

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Research Square 2020年

作者： Liao, Chi-Chou Chen, Liang-Jwu Lo, Shuen-Fang Chen, Chi-Wei Chen, Jia-Jyun Chu, Yen-Wei Institute of Molecular Biology National Chung Hsing University Taichung Taiwan Agricultural Biotechnology Center National Chung Hsing University Taichung Taiwan Institute of Molecular Biology Academia Sinica Taipei Taiwan Department of Computer Science and Engineering National Chung Hsing University Taichung Taiwan Institute of Genomics and Bioinformatics National Chung Hsing University Taichung Taiwan Biotechnology Center National Chung Hsing University Taichung Taiwan Ph.D. Program in Translational Medicine National Chung Hsing University Taichung Taiwan Rong Hsing Research Center for Translational Medicine National Chung Hsing University Taichung402 Taiwan

Background: T-dNA activation-tagging technology is widely used to enhance flanking gene expression near the site of insertion for functional genomics research in rice. However, whether the expression of a gene of interest is enhanced must be validated experimentally. Results: In this study, we built a model to predict gene expression in T-dNA mutants by machine learning approaches, thereby improving the efficiency of screening for activated genes. We gathered experimental consisting of gene expression data in T-dNA mutants and captured the PROMOTER and MIddLE sequences for encoding. In first-layer models, SVM models were constructed with nine features consisting of information about biological function and local and global sequences. Feature-encoding based on the PROMOTER sequence was weighted by logistic regression. The second-layer models integrated 16 first-layer models with feature selection and the algorithm, which were selected from nine feature selection methods and 65 classified methods, respectively. The accuracy of the final two-layer machine learning model, referred to as was 99.3% based on fivefold cross-validation, and 85.6% based on independent-testing. Conclusion: We discovered that the information within the local sequence had a greater contribution than the global sequence with respect to classification had a good predictive ability for target genes within 20 from the 35S enhancer. Based on the analysis of significant sequences, the G-box regulatory sequence may also play an important role in the mechanism of activation of the 35S enhancer. © 2020, CC BY.

关键词： Gene expression

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