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The use of mobile applicatiosn to improve nutrition behaviour: A systematic review

Computer Methods and programs in Biomedicine 2020年 192卷

作者： Paramastri, Rathi Pratama, Satwika Arya Ho, Dang Khanh Ngan Purnamasari, Sintha Dewi Mohammed, Afrah Zaki Galvin, Cooper J Hsu, Yi-Hsin Elsa Tanweer, Afifa Humayun, Ayesha Househ, Mowafa Iqbal, Usman School of Nutrition and Health Sciences College of Nutrition Taipei Medical University Taipei Taiwan Taipei Medical University Taipei Taiwan School of Health Care Administration College of Management Taipei Medical University Taipei Taiwan Executive Master Program of Business Administration in Biotechnology College of Management Taipei Medical University Taipei Taiwan School of Health Sciences University of Management and Technology Lahore Pakistan Department of Public Health and Community Medicine Shaikh Zayed Medical Complex Lahore Pakistan SKZMDC Shaikh Khalifa Bin Zayed Al-Nahyan Medical College Shaikh Zayed Postgraduate Medical Institute Shaikh Zayed Medical Complex Lahore Pakistan Hamad Bin Khalifa University College of Science and Engineering Qatar Foundation Qatar School of Health Information Science University of Victoria VictoriaBC Canada Master's Program in Global Health & Development Dept. PhD Program in Global Health & Health Security Dept. College of Public Health Taipei Medical University Taipei Taiwan

Background and Objective: Mobile applications could be effectively used for dietary intake assessment, physical activity monitoring, behavior improvement, and nutrition education. The aim of this review is to determine the effectiveness of mobile applications in improving nutrition behaviors through a systematic review of literature. Methods: The review protocol was registered with PROSPERO: registration number CRD42018118809, and followed PRISMA guidelines. We involved original articles including mobile electronic devices for improving dietary intake, physical activity, and weight management in adult populations in this review. Data were retrieved from January 2010 to December 2018 with PubMed, Web of Science, Excerpta Medica Database (Embase), and Cumulative Index to Nursing and Allied Health Literature (CINAHL) as data sources. Authors individually screened the titles and abstracts, then full articles in order to obtain papers that met inclusion criteria. Results: The database search yielded 2962 records. After removing the duplicates and analyzing the full text papers a total of 8 original articles were reviewed. Two articles showed obvious bias and were not included in our results or discussion. The remaining six articles with low to moderate bias risk were included in this systematic review. Three selected studies were randomized control trials (RCTs) with over 180 participants each. The other three studies were a nested trial, a case-control trial, and a pilot RCT with 36, 162, and 24 participants respectively. All larger RCTs and the small case control trail showed significant improvements in some nutritional-health objectives measured. The other two trials showed insignificant improvements in outcomes measured between groups. Conclusion: This study highlights the potential significant health benefits acquirable through mobile health application-assisted nutrition interventions. Some of these studies required significant financial and time input from provide

关键词： Nutrition

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Sustainable flower-like Cu-Ni metal-organic frameworks catalyst for enhanced electrochemical sensing and photocatalytic degradation of antibiotic pollutants

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Microchemical Journal 2025年 214卷

作者： N.S.K. Gowthaman Ameer Farithkhan E. Narayanamoorthi Ponnusamy Arul Chinnathambi Nandhini Vellaichamy Balakumar S. Selvarajan School of Engineering Monash University Bandar Sunway Selangor 47500 Malaysia Center for Advanced Energy Materials SRM TRP Engineering College Tiruchirapalli 621 105 Tamil Nadu India Center for Research Easwari Engineering College Chennai 600 089 Tamil Nadu India School of Materials Science and Engineering Pusan National University Busan 46241 Republic of Korea International PhD Program in Innovative Technology of Biomedical Engineering and Medical Devices Ming Chi University of Technology New Taipei City 243303 Taiwan Institute of Biochemical and Biomedical Engineering Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei 106 Taiwan Department of Materials Engineering Ming Chi University of Technology New Taipei City 24303 Taiwan Department of Chemistry Sri Ramakrishna College of Arts & Science Coimbatore 641 006 Tamil Nadu India Department of Chemistry Theni Kammavar Sangam College of Arts & Science Theni 625 531 Tamil Nadu India

Massive usage of antibiotics harms the environment through animals’ excrement and squeezes open-wellbeing issues. Enrofloxacin (EF), an antimicrobial agent, treats bacterial diseases and infections in cattle, poultry and exotic animals and exhibits harmful side effects. The present work proposed a sensitive and rapid electrochemical scaffold based on the Cu-Ni bimetallic-organic framework (Cu-Ni-MOF) for the detection of harmful EF in real-time applications. The Cu-Ni-MOF was synthesized through solvothermal approach with the support of a multifunctional organic ligand, 4,4-biphenyldicarboxylicacid and characterized through various analytical tools. The electrochemical sensor for EF detection was constructed by drop-casting the Cu-Ni-MOF on a glassy carbon electrode and the fabricated sensor exhibited higher catalytic activity when compared to the parental counterparts by exhibiting 1.7-fold higher current for EF oxidation. Further, the fabricated sensor is highly active in the linear range response of 200 nM–2 mM with a limit of detection of 15.17 nM and sensitivity of 638.71 µA mM cm −2 under optimized environments. The higher electrocatalytic activity of the Cu-Ni-MOF electrode synergistically stems from the strong interaction between Cu 2+ of the MOF and pyridone and carboxylate moieties of the EF and facile electron transition facilitated by Ni 2+ . The practicability of the sensor was evaluated in milk, egg and river water samples and the obtained results were validated with Liquid Chromatography-Mass Spectrometric technique. Furthermore, the prepared catalyst was utilized for the photochemical decontamination of EF and the present Cu-Ni-MOF catalyst delivered excellent photocatalytic activity in the decontamination of EF under visible light at ambient temperature.

关键词： Cu-Ni-MOF Electrochemical sensor Enrofloxacin Environmental pollutants Metal-organic framework Photocatalytic degradation

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Computer-aided drug discovery of a dual-target inhibitor for ovarian cancer: therapeutic intervention targeting CDK1/TTK signaling pathway and structural insights in the NCI-60

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Computers in biology and medicine 2025年 193卷 110445页

作者： Sung-Ling Tang Maryam Rachmawati Sumitra Yu-Cheng Kuo Han-Lin Hsu Muhamad Ansar Sheng-Liang Huang Shih-Yu Lee Hong-Jaan Wang Bashir Lawal Alexander T H Wu Lung-Ching Chen Hsu-Shan Huang School of Pharmacy National Defense Medical Center Taipei 11490 Taiwan. PhD Program for Cancer Molecular Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan Graduate Institute of Cancer Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan. Department of Pharmacology School of Medicine College of Medicine Taipei Medical University Taipei 11031 Taiwan School of Post-Baccalaureate Chinese Medicine College of Chinese Medicine China Medical University Taichung 40604 Taiwan. Graduate Institute of Clinical Medicine Taipei Medical University Taipei 11031 Taiwan Division of Pulmonary Medicine Department of Internal Medicine Wanfang Hospital Taipei Medical University Taipei 11031 Taiwan. Ph.D. Program in the Clinical Drug Development of Herbal Medicine Taipei Medical University Taipei 110301 Taiwan. Graduate Institute of Aerospace and Undersea Medicine National Defense Medical Center Taipei 11490 Taiwan. UPMC Hillman Cancer Center University of Pittsburgh Pittsburgh PA USA. Ph.D. Program of Translational Medicine College of Science and Technology Taipei Medical University Taipei 11031 Taiwan. Division of Cardiology Department of Internal Medicine Shin Kong Wu Ho-Su Memorial Hospital Taipei 11101 Taiwan. School of Pharmacy National Defense Medical Center Taipei 11490 Taiwan Graduate Institute of Cancer Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan Ph.D. Program in Biotechnology Research and Development College of Pharmacy Taipei Medical University Taipei 11031 Taiwan. Electronic address: huanghs99@tmu.edu.tw.

Ovarian cancer remains the third most prevalent and deadliest gynecologic malignancy worldwide, with most patients eventually developing resistance to platinum-based chemotherapy. This highlights a critical unmet need for innovative multitargeted therapies to address current treatment challenges. In this study, we identified 35 differentially expressed genes (DEGs) through integrated analysis of four GEO ovarian cancer datasets, with validation using TCGA data. Gene Ontology (GO) and KEGG enrichment analyses highlighted key tumor-associated pathways, and protein-protein interaction (PPI) network modeling prioritized CDK1 and TTK as high-value therapeutic targets. We evaluated the association between molecular genomic features and drug responses across the NCI-60 ovarian cancer cell line panel (IGROV1, OVCAR-3, OVCAR-4, OVCAR-5, OVCAR-8, NCI/ADR-RES, and SK-OV-3), using a series of salicylanilide-derived compounds and four FDA-approved drugs (cabozantinib, paclitaxel, rapamycin, and niclosamide) from the NCI Developmental Therapeutics program (DTP). Among these, NSC765690 (MCC22) emerged as the most promising candidate. It demonstrated potent antiproliferative activity, high target selectivity, and strong binding affinity to both CDK1 and TTK. Multi-omics integration, combined with AI-driven network modeling, further elucidated NSC765690's mechanism of action and its relevance to ovarian cancer pathogenesis. Additionally, ADMET and pharmacokinetic profiling confirmed its favorable drug-like properties and low predicted toxicity. Collectively, these findings establish NSC765690 as a potent dual-target inhibitor and exemplify a rational, data-driven drug discovery pipeline for overcoming chemotherapy resistance in ovarian cancer.

关键词： Dual-target small molecule inhibitor Machine learning Molecular docking Molecular dynamics simulations NCI-60 panel Ovarian cancer

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Corrigendum to “Metal ratio and bimetal nanoarchitectonics of ammonia-based fluoride complex induced nickel hydroxide and manganese oxide composites as active materials of an energy storage device” [J. Energy Storage 80 (2024) 110316]

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Journal of Energy Storage 2025年 108卷

作者： Chuan-Chih Hsu You Zher Yu Chung-Hsien Wu Pin-Yan Lee Hung-Ming Chen Sadang Husain Chutima Kongvarhodom Yu-Cheng Hsiao Lu-Yin Lin Division of Cardiovascular Surgery Department of Surgery School of Medicine College of Medicine Taipei Medical University Taiwan Division of Cardiovascular Surgery Department of Surgery Taipei Medical University Hospital Taiwan Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei Taiwan Gingen Technology Co. LTD. Rm. 7 10F. No.189 Sec. 2 Keelung Rd. Xinyi Dist. Taipei 11054 Taiwan Department of Physics Faculty of Mathematics and Natural Science Lambung Mangkurat University Banjarmasin 70124 Indonesia Department of Chemical Engineering King Mongkut's University of Technology Thonburi 126 Pracha-u-thit Toong-kru Bangkok 10140 Thailand Cell Physiology and Molecular Image Research Center Wan Fang Hospital Taipei Medical University Taipei 11696 Taiwan International PhD Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei 11031 Taiwan Stanford Byers Center for Biodesign Stanford University CA 94305-5428 USA Graduate Institute of Biomedical Optomechatronics College of Biomedical Engineering Taipei Medical University Taipei 11031 Taiwan

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Correction to: Disturbed Gastrointestinal Contractility in a Polycystic Ovary Syndrome Rat Model

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Digestive diseases and sciences 2020年第11期65卷 3408-3409页

作者： Kai-Lee Wang Shih-Min Hsia Paulus S Wang Po-Han Lin Department of Nursing Ching Kuo Institute of Management and Health No. 336 Fuxsin Rd. Zhongshan Dist. Keelung City 20301 Taiwan Republic of China. d49505002@gm.ym.edu.tw. Department of Physiology School of Medicine National Yang-Ming University Taipei Taiwan Republic of China. d49505002@gm.ym.edu.tw. School of Nutrition and Health Sciences Taipei Medical University Taipei 11031 Taiwan Republic of China. Department of Physiology School of Medicine National Yang-Ming University Taipei Taiwan Republic of China. Department of Medical Research and Education Taipei Veterans General Hospital Taipei Taiwan Republic of China. PhD Program of Aging College of Medicine China Medical University Taichung Taiwan Republic of China. Department of Biotechnology Asia University Taichung Taiwan Republic of China.

The original version of the article unfortunately contained an error in the legend of Figure 5B. Corrected version of Figure 5 is given below.

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The genome-wide association study of serum IgE levels demonstrated a shared genetic background in allergic diseases

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Clinical immunology (Orlando, Fla.) 2024年 260卷 109897页

作者： Hsing-Fang Lu Chen-Hsing Chou Ying-Ju Lin Shunsuke Uchiyama Chikashi Terao Yu-Wen Wang Jai-Sing Yang Ting-Yuan Liu Henry Sung-Ching Wong Sean Chun-Chang Chen Fuu-Jen Tsai Department of Medical Research China Medical University Hospital Taichung 404327 Taiwan Laboratory for Statistical and Translational Genetics RIKEN Center for Integrative Medical Sciences Yokohama Japan. PhD Program for Health Science and Industry College of Health Care China Medical University Taichung Taiwan Department of Health Services Administration China Medical University Taichung Taiwan. Genetic Center Department of Medical Research China Medical University Hospital Taichung Taiwan School of Chinese Medicine China Medical University Taichung Taiwan. Laboratory for Statistical and Translational Genetics RIKEN Center for Integrative Medical Sciences Yokohama Japan Clinical Research Center Shizuoka General Hospital Shizuoka Japan The Department of Applied Genetics The School of Pharmaceutical Sciences University of Shizuoka Shizuoka Japan. Department of Medical Research China Medical University Hospital Taichung 404327 Taiwan. Department of Medical Research China Medical University Hospital China Medical University Taichung 404327 Taiwan. Graduate Institute of Biomedical Informatics College of Medical Science and Technology Taipei Medical University Taipei Taiwan. School of Chinese Medicine China Medical University Taichung Taiwan Department of Biotechnology and Bioinformatics Asia University Taichung Taiwan. Electronic address: d0704@mail.cmuh.org.tw.

Immunoglobulin E (IgE) synthessis is highly related to a variety of atopic diseases, and several genome-wide association studies (GWASs) have demonstrated the association between genes and IgE level. In this study, we conducted the largest genome-wide association study of IgE involving a Taiwanese Han population. Eight independent variants exhibited genome-wide significance. Among them, an intronic SNP of CD28, rs1181388, and an intergenic SNP, rs1002957030, on 11q23.2 were identified as novel signals for IgE. Seven of the loci were replicated successfully in a meta-analysis using data on Japanese population. Among all the human leukocyte antigen (HLA) regions, HLA-DQA1*03:02 - HLA-DQB1*03:03 was the most significant haplotype (OR = 1.25, SE = 0.02, FDR = 1.6 × 10), corresponding to HLA-DQA1 Asp160 and HLA-DQB1 Leu87 amino acid residues. The genetic correlation showed significance between IgE and allergic diseases including asthma, atopic dermatitis, and pollinosis. IgE PRS was significantly correlated with total IgE levels. Furthermore, the top decile IgE polygenic risk score (PRS) group had the highest risk of asthma for the Taiwan Biobank and Biobank Japan cohorts. IgE PRS may be used to aid in predicting the occurrence of allergic reactions before symptoms occur and biomarkers are detectable. Our study provided a more comprehensive understanding of the impact of genomic variants, including complex HLA alleles, on serum IgE levels.

关键词： Allergy genome-wide association study human leukocyte antigen immunoglobulin E

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Author Correction: Proteome-wide non-cleavable crosslink identification with MS Annika 3.0 reveals the structure of the C. elegans Box C/D complex

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Communications chemistry 2025年第1期8卷 62页

作者： Micha J Birklbauer Fränze Müller Sowmya Sivakumar Geetha Manuel Matzinger Karl Mechtler Viktoria Dorfer Bioinformatics Research Group University of Applied Sciences Upper Austria Softwarepark 11 Hagenberg 4232 Austria. micha.birklbauer@fh-hagenberg.at. Institute for Symbolic Artificial Intelligence Johannes Kepler University Linz Altenberger Straße 69 Linz 4040 Austria. micha.birklbauer@fh-hagenberg.at. Institute of Molecular Pathology (IMP) Vienna BioCenter (VBC) Campus-Vienna-Biocenter 1 Vienna 1030 Austria. Max Perutz Labs (MPL) Vienna BioCenter (VBC) Dr. Bohr-Gasse 9/Vienna Biocenter 5 Vienna 1030 Austria. Max Perutz Labs (MPL) Department of Chromosome Biology University of Vienna Dr. Bohr-Gasse 9/Vienna Biocenter 5 Vienna 1030 Austria. Vienna BioCenter PhD Program a Doctoral School of the University of Vienna and the Medical University of Vienna Vienna BioCenter (VBC) Dr. Bohr-Gasse 9/Vienna Biocenter 5 Vienna 1030 Austria. Institute of Molecular Biotechnology (IMBA) Austrian Academy of Sciences Vienna BioCenter (VBC) Dr. Bohr-Gasse 3 Vienna 1030 Austria. Gregor Mendel Institute (GMI) Austrian Academy of Sciences Vienna BioCenter (VBC) Dr. Bohr-Gasse 3 Vienna 1030 Austria. Bioinformatics Research Group University of Applied Sciences Upper Austria Softwarepark 11 Hagenberg 4232 Austria. viktoria.dorfer@fh-hagenberg.at.

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Strategic modification of low-activity natural antimicrobial peptides confers antibacterial potential in vitro and in vivo

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European journal of medicinal chemistry 2023年 249卷 115131页

作者： Prakash Kishore Hazam Chih-Cheng Cheng Wen-Chun Lin Chu-Yi Hsieh Po-Hsien Hsu Yun-Ru Chen Chao-Chin Li Po-Ren Hsueh Jyh-Yih Chen Marine Research Station Institute of Cellular and Organismic Biology Academia Sinica 23-10 Dahuen Rd. Jiaushi Ilan 262 Taiwan. Institute of Fisheries Science National Taiwan University 1 Roosevelt Road Sec. 4 Taipei 106 Taiwan. Academia Sinica Protein Clinic Institute of Biological Chemistry Academia Sinica 128 Academia Road Section 2 Nankang District Taipei 115 Taiwan. Institute of Cellular and Organismic Biology Academia Sinica Nankang Taipei 115 Taiwan. Departments of Laboratory Medicine and Internal Medicine China Medical University Hospital School of Medicine China Medical University Taichung Taiwan PhD Program for Aging School of Medicine China Medical University Taichung Taiwan Departments of Laboratory Medicine and Internal Medicine National Taiwan University Hospital National Taiwan University College of Medicine Taipei Taiwan. Marine Research Station Institute of Cellular and Organismic Biology Academia Sinica 23-10 Dahuen Rd. Jiaushi Ilan 262 Taiwan The iEGG and Animal Biotechnology Center and the Rong Hsing Research Center for Translational Medicine National Chung Hsing University Taichung 402 Taiwan. Electronic address: zoocjy@gate.sinica.edu.tw.

Antimicrobial peptides (AMPs) show great promise for clinical applications, but the utility of naturally occurring AMPs is often limited by their stability. Here, we used a rational design approach to improve the characteristics of a pair of inactive peptides, tilapia piscidin 1 and 2 (TP1 and TP2). From each starting peptide, we generated a series of novel derivatives by substituting residues to adjust cationic charge density, percent hydrophobicity and hydrophilicity/hydrophobicity coefficients. This approach yielded a novel peptide, TP2-5 (KKCIAKAILKKAKKLLKKLVNP), that exhibits significant bactericidal potency, low cytotoxicity and high stability. The designed peptide further showed antibiofilm activity, rapid antibacterial action and a low capacity to induce bacterial resistance. Importantly, we also demonstrated that TP2-5 can protect mice in a Vibrio vulnificus-infected wound model. Therefore, our peptide modification strategy successfully generated a novel AMP with high potential for future clinical application.

关键词： Antimicrobial peptides (AMPs) Multidrug-resistant (MDR) bacteria Murine wound infection model Peptide drug design Tilapia piscidins (TPs) Vibrio vulnificus

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Distinct viral reservoirs and immune signatures in individuals on long-term antiretroviral therapy with perinatally acquired HIV-1

Cell reports. Medicine

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Cell reports. Medicine 2025年 102150页

作者： Benjamin Bone Nicola Cotugno Chiara Pighi Arianna Rotili Seohyun Hong Leah Carrere Elena Morrocchi Giuseppe Rubens Pascucci Ce Gao Nicole Colantoni Weiwei Sun Giovanna Leone David R Collins Mpho J Olatotse Giovanna Del Principe Toong Seng Tan Melanie Lancien Alessia Neri Libera Sessa Giulio Olivieri Kailey Shapiro Isabelle Roseto Catherine Koofhethile Elena Emili Stefania Bernardi Ann Chahroudi Paolo Rossi Bruce D Walker Xu G Yu Mathias Lichterfeld Paolo Palma The Ragon Institute of MGH MIT and Harvard Boston MA USA Infectious Disease Division Brigham Women's Hospital Boston MA USA. Electronic address: brb173@med.miami.edu. Clinical Immunology and Vaccinology Unit IRCCS Bambino Gesù Children's Hospital Rome Italy Chair of Paediatrics Department of Systems Medicine University of Rome "Tor Vergata" Rome Italy. Electronic address: nicola.cotugno@***. Clinical Immunology and Vaccinology Unit IRCCS Bambino Gesù Children's Hospital Rome Italy. PhD Program in Immunology Molecular Medicine and Applied Biotechnology University of Rome "Tor Vergata" Rome Italy. The Ragon Institute of MGH MIT and Harvard Boston MA USA. Infectious Disease Division Brigham Women's Hospital Boston MA USA. Chair of Paediatrics Department of Systems Medicine University of Rome "Tor Vergata" Rome Italy. Transfusion Unit IRCCS Bambino Gesù Children's Hospital Rome Italy. Infectious Diseases Unit IRCCS Bambino Gesù Children's Hospital Rome Italy. Department of Pediatrics Emory University School of Medicine Atlanta GA USA. Infectious Disease Division Brigham Women's Hospital Boston MA USA. Electronic address: mlichterfeld@mgh.harvard.edu. Chair of Paediatrics Department of Systems Medicine University of Rome "Tor Vergata" Rome Italy. Electronic address: paolo.palma@***.

Early initiation of antiretroviral therapy (ART) following HIV-1 infection restricts the size of the latent reservoir, following both horizontal and vertical infections. Here, we comprehensively profile the reservoirs and immunological milieus of nine young adults who acquired HIV-1 perinatally and remained on suppressive long-term ART (median: 20 years) since infancy (LeukoHIV cohort). Genome-intact reservoirs are markedly smaller compared to a cohort of adults on suppressive ART started in adulthood, with some LeukoHIV individuals characterized by an absence or near absence of intact proviruses in up to a billion peripheral blood mononuclear cells (PBMCs). Higher frequencies of functional CD56 natural killer (NK) cells with increased cytotoxic activity are detectable in the LeukoHIV cohort compared to an adult reference cohort, while one LeukoHIV participant displayed a potent HIV-1-specific CD8 T cell response. Collectively, our data suggest that long-term ART initiated in early life following perinatal transmission may facilitate an immune environment better equipped to restrict the HIV-1 reservoir.

关键词： HIV MIP-seq children deep latency leukophoresis long-term ART natural killer cells perinatal infection provirus reservoir

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Greenhouse Gas Emissions and Growth of Wheat Cultivated in Soil Amended with Digestate from Biogas Production

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Pedosphere 2017年第2期27卷 318-327页

作者： Liliana PAMPILLON-GONZALEZ Marco LUNA-GUIDO Victor Manuel RUIZ-VALDIVIEZO Olivia FRANCO-HERNANDEZ Fabign FERNANDEZ-LUQUENO Oetavio PAREDES-LOPEZ Gerardo HERNANDEZ Luc DENDOOVEN PhD Program on Science Technology and Society Cinvestav IPN 2508 Mexico DF 07360 (Mexico) Laboratory of Soil Ecology Laboratorio de Matemdticas Aplicadas y Cdmputo de Alto Rendimiento Cinvestav-Edomex (ABACUS) Cinvestav IPN 2508 Mexico DF 07360 (Mexico) Department of Chemistry Unidad Profesional Interdisciplinaria de Biotecnologla-Instituto Politdcnico Nacional (UPIBI-IPN) Me- xico DF 07340 (Mexico) Sustainability of Natural Resources and Energy Program Cinvestav-Saltillo 1062 Coahuila 25900 (Mexico) Department of Biotechnology and Biochemistry Cinvestav Irapuato 36821 (Mexico)

Digestate, the product obtained after anaerobic digestion of organic waste for biogas production, is rich in plant nutrients and might be used to fertilize crops. Wheat （Triticum spp. L.） was fertilized with digestate, urea, or left unfertilized and cultivated in the greenhouse for 120 d. Emissions of greenhouse gasses （carbon dioxide （CO2）, methane （CH4）, and nitrous oxide （N20）） were monitored and plant growth characteristics were determined at harvest. The digestate was characterized for heavy metals, pathogens, and C and N mineralization potential in an aerobic incubation experiment. No Salmonella spp., Shigella spp., or viable eggs of helminths were detected in the digested pig slurry, but the number of faecal coliforms was as high as 3.6 ~ 104 colony-forming units （CFU） g-1 dry digestate. The concentrations of heavy metals did not surpass the upper limits established by US Environmental Protection Agency （EPA）. After 28 d, 17% of the organic C （436 g kg-1 dry digestate） and 8% of the organic N （6.92 g kg-1 dry digestate） were mineralized. Emissions of CO2 and CH4 were not significantly affected by fertilization in the wheat-cultivated soil, but digestate significantly increased the cumulative N20 emission by 5 times compared to the urea-amended soil and 63 times compared to the uncultivated unfertilized soil. It could be concluded that digestate was nutrient rich and low in heavy metals and pathogens, and did not affect emissions of CH4 and CO2 when applied to a soil cultivated with wheat, but increased emission of N20. Key Words： biodigester, C and N mineralization potential, faecal coliform, heavy metal, pathogen, pig slurry

关键词： biodigester C and N mineralization potential faecal coliform heavy metal pathogen pig slurry

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