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Journal of Extracellular Biology 2022年第10期1卷 e63页

作者： Paolini, Lucia Monguió-Tortajada, Marta Costa, Marta Antenucci, Fabio Barilani, Mario Clos-Sansalvador, Marta Andrade, André Cronemberger Driedonks, Tom A. P. Giancaterino, Sara Kronstadt, Stephanie M. Mizenko, Rachel R. Nawaz, Muhammad Osteikoetxea, Xabier Pereira, Carla Shrivastava, Surya Boysen, Anders Toftegaard van de Wakker, Simonides Immanuel van Herwijnen, Martijn J. C. Wang, Xiaoqin Watson, Dionysios C. Gimona, Mario Kaparakis-Liaskos, Maria Konstantinov, Konstantin Lim, Sai Kiang Meisner-Kober, Nicole Stork, Michiel Nejsum, Peter Radeghieri, Annalisa Rohde, Eva Touzet, Nicolas Wauben, Marca H. M. Witwer, Kenneth W. Bongiovanni, Antonella Bergese, Paolo Department of Molecular and Translational Medicine University of Brescia Brescia Italy Center for Colloid and Surface Science (CSGI) Department of Chemistry University of Florence Sesto Fiorentino (FI) Italy Department of Medical and Surgical Specialties Radiological Sciences and Public Health University of Brescia Brescia Italy CREC Research Program Health Science Research Institute Germans Trias i Pujol (IGTP) Badalona Spain Heart Institute (iCor) Cardiology Department Germans Trias i Pujol University Hospital Badalona Spain iBET Instituto de Biologia Experimental e Tecnológica Oeiras Portugal Instituto de Tecnologia Química e Biológica António Xavier Universidade Nova de Lisboa Oeiras Portugal Department of Veterinary and Animal Sciences University of Copenhagen Copenhagen Denmark Laboratory of Regenerative Medicine ‒ Cell Factory Department of Transfusion Medicine and Hematology Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano Italy REMAR-IGTP Group Germans Trias i Pujol Research Institute (IGTP) & Nephrology Department University Hospital Germans Trias i Pujol (HUGTiP) Catalonia Badalona (Barcelona) Spain Department of Cell Biology Physiology and Immunology Universitat Autònoma de Barcelona (UAB) Bellaterra Spain Experimental and Clinical Cell Therapy Institute (ExCT) Spinal Cord Injury and Tissue Regeneration Center Paracelsus Medical University Salzburg Austria CDL Research University Medical Center Utrecht Utrecht University Utrecht Netherlands Department of Molecular and Comparative Pathology Johns Hopkins School of Medicine Baltimore MD United States Department of Civil Chemical Environmental and Materials Engineering Alma Mater Studiorum University of Bologna Bologna Italy Fischell Department of Bioengineering University of Maryland College Park MD United States Department of Biomedical Engineering University of California Davis CA United States Department of Rheumatology and Inflammation Research Institute of Medicine

Extracellular vesicles (EVs) large-scale production is a crucial point for the translation of EVs from discovery to application of EV-based products. In October 2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET-OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY) and “Extracellular vesicles from a natural source for tailor-made nanomaterials” (VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV-based products. This report gives an overview of the topics discussed during “massivEVs”, the most important points raised, and the points of consensus reached after discussion among academia and industry representatives. Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future for the expanding EV field. © 2022 The Authors. Journal of Extracellular Biology published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.

关键词： EV-based products extracellular vesicles ISEV workshop large-scale production manufacturing processing regulatory issues

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Patterns of somatic structural variation in human cancer genomes (vol 578, pg 112, 2020)

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NATURE 2023年第7948期614卷 E38-E38页

作者： Li, Yilong Roberts, Nicola D. Wala, Jeremiah A. Shapira, Ofer Schumacher, Steven E. Kumar, Kiran Khurana, Ekta Waszak, Sebastian Korbel, Jan O. Haber, James E. Imielinski, Marcin Weischenfeldt, Joachim Beroukhim, Rameen Campbell, Peter J. Cancer Genome Project Wellcome Trust Sanger Institute Hinxton UK Totient Inc Cambridge MA USA Wellcome Sanger Institute Wellcome Genome Campus Hinxton UK Department of Haematology University of Cambridge Cambridge UK Cambridge University Hospitals NHS Foundation Trust Cambridge UK Korea Advanced Institute of Science and Technology Daejeon South Korea Department of Zoology Genetics and Physical Anthropology University of Santiago de Compostela Santiago de Compostela Spain Centre for Research in Molecular Medicine and Chronic Diseases (CIMUS) University of Santiago de Compostela Santiago de Compostela Spain The Biomedical Research Centre (CINBIO) University of Vigo Vigo Spain Centre for Research in Molecular Medicine and Chronic Diseases (CiMUS) Universidade de Santiago de Compostela Santiago de Compostela Spain Department of Zoology Genetics and Physical Anthropology (CiMUS) Universidade de Santiago de Compostela Santiago de Compostela Spain The Biomedical Research Centre (CINBIO) Universidade de Vigo Vigo Spain The Broad Institute of Harvard and MIT Cambridge MA USA Bioinformatics and Integrative Genomics Harvard University Cambridge MA USA Department of Cancer Biology Dana-Farber Cancer Institute Boston MA USA Broad Institute of MIT and Harvard Cambridge MA USA Department of Medical Oncology Dana-Farber Cancer Institute Boston MA USA Harvard Medical School Boston MA USA Massachusetts General Hospital Center for Cancer Research Charlestown MA USA Dana-Farber Cancer Institute Boston MA USA Department of Biostatistics and Computational Biology Dana-Farber Cancer Institute and Harvard Medical School Boston MA USA Weill Cornell Medical College New York NY USA Department of Physiology and Biophysics Weill Cornell Medicine New York NY USA Institute for Computational Biomedicine Weill Cornell Medicine New York NY USA Controlled Department and Institution New York NY USA Englander Institute for Precision Medicine Weill Cornell Medicine Ne

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Molecular landscape of sex- and modality-specific exercise adaptation in human skeletal muscle through large-scale multi-omics integration

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Cell Reports 2025年第6期44卷 115750页

作者： Jacques, Macsue Landen, Shanie Sharples, Adam P. Garnham, Andrew Schittenhelm, Ralf Steele, Joel Heikkinen, Aino Sillanpää, Elina Ollikainen, Miina Broatch, James Zarekookandeh, Navabeh Hanson, Ola Ekström, Ola Asplund, Olof Lamon, Séverine Alexander, Sarah E. Smith, Cassandra Bauer, Carlie Woessner, Mary N. Levinger, Itamar Teschendorff, Andrew E. Gillberg, Linn Blom, Ida Helge, Jørn Wulff Harvey, Nicholas R. Haupt, Larisa M. Griffiths, Lyn R. Deshmukh, Atul S. Pietiläinen, Kirsi H. Piirilä, Päivi Seaborne, Robert A.E. Klevjer, Marie Bye, Anja Wisløff, Ulrik Jones-Freeman, Bernadette Eynon, Nir Australian Regenerative Medicine Institute Monash University Clayton VIC Australia Institute for Health and Sport (iHeS) Victoria University Footscray VIC Australia Centre for Endocrinology and Metabolism Hudson Institute of Medical Research Melbourne VIC Australia Institute of Physical Performance Norwegian School of Sport Sciences Oslo Norway Monash Proteomics & Metabolomics Platform Monash Biomedicine Discovery Institute Monash University Melbourne VIC Australia Institute for Molecular Medicine Finland (FIMM) Helsinki University Helsinki Finland Faculty of Sport and Health Sciences University of Jyväskylä Jyväskylä Finland Wellbeing Services County of Central Finland Jyväskylä Finland Minerva Foundation Institute for Medical Research Helsinki Finland Department of Clinical Sciences Translational Muscle Research Unit Lund University Diabetes Center Lund University Lund Sweden Institute for Physical Activity and Nutrition (IPAN) School of Exercise and Nutrition Sciences Deakin University 221 Burwood Highway Burwood 3125 VIC Australia Cardiometabolic Health and Exercise Physiology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road Melbourne 3004 VIC Australia Nutrition & Health Innovation Research Institute Edith Cowan University Joondalup WA Australia Medical School The University of Western Australia Perth WA Australia Australian Institute for Musculoskeletal Science (AIMSS) Victoria University Melbourne VIC Australia CAS Key Lab of Computational Biology Shanghai Institute of Nutrition and Health Shanghai China Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark Faculty of Health Sciences and Medicine Bond University Gold Coast QLD Australia Genomics Research Centre Centre for Genomics and Personalised Health School of Biomedical Sciences Queensland University of Technology Brisbane QLD Australia ARC Training Centre for Cell and Tissue Engineering Technologies Queensland University of Technology

We investigated the molecular mechanisms of exercise adaptations in human muscle by integrating genome, methylome, transcriptome, and proteome data from over 1,000 participants (2,340 muscle samples). We identified distinctive signatures associated with maximal oxygen consumption (VO_2max), and multi-omics integration uncovered five key genes as robust exercise markers across layers, with transcription factors functioning as activators, synergizing with DNA methylation to regulate gene expression. Minimal sex differences were observed, while modality-specific analysis highlighted distinct pathways for aerobic and resistance exercise, contrasting with muscle disuse patterns. Finally, we created a webtool, OMAx, featuring our individual omics and integration analysis. These findings provide a comprehensive multi-omics framework for understanding exercise-induced molecular adaptations, offering insights into muscle health, cardiorespiratory fitness, and their roles in aging and disease prevention. © 2025 The Author(s)

关键词： CP: Metabolism DNA methylation epigenetics exercise gene expression Multi Omics proteomic skeletal muscle transcriptomic VO2 max

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The evolutionary history of 2,658 cancers (vol 578, pg 122, 2020)

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NATURE 2023年第7948期614卷 E42-E42页

作者： Gerstung, Moritz Jolly, Clemency Leshchiner, Ignaty Dentro, Stefan C. Gonzalez, Santiago Rosebrock, Daniel Mitchell, Thomas J. Rubanova, Yulia Anur, Pavana Yu, Kaixian Tarabichi, Maxime Deshwar, Amit Wintersinger, Jeff Kleinheinz, Kortine Vazquez-Garcia, Ignacio Haase, Kerstin Jerman, Lara Sengupta, Subhajit Macintyre, Geoff Malikic, Salem Donmez, Nilgun Livitz, Dimitri G. Cmero, Marek Demeulemeester, Jonas Schumacher, Steven Fan, Yu Yao, Xiaotong Lee, Juhee Schlesner, Matthias Boutros, Paul C. Bowtell, David D. Zhu, Hongtu Getz, Gad Imielinski, Marcin Beroukhim, Rameen Sahinalp, S. Cenk Ji, Yuan Peifer, Martin Markowetz, Florian Mustonen, Ville Yuan, Ke Wang, Wenyi Morris, Quaid D. Spellman, Paul T. Wedge, David C. Van Loo, Peter European Molecular Biology Laboratory European Bioinformatics Institute (EMBL-EBI) Cambridge UK European Molecular Biology Laboratory Genome Biology Unit Heidelberg Germany Wellcome Sanger Institute Cambridge UK Genome Biology Unit European Molecular Biology Laboratory (EMBL) Heidelberg Germany University of Ljubljana Ljubljana Slovenia The Francis Crick Institute London UK Big Data Institute University of Oxford Oxford UK Wellcome Sanger Institute Wellcome Genome Campus Hinxton UK Big Data Institute Li Ka Shing Centre University of Oxford Oxford UK University of Cambridge Cambridge UK Cambridge University Hospitals NHS Foundation Trust Cambridge UK Department of Applied Mathematics and Theoretical Physics Centre for Mathematical Sciences University of Cambridge Cambridge UK Department of Epidemiology and Biostatistics Memorial Sloan Kettering Cancer Center New York NY USA Department of Statistics Columbia University New York NY USA Department of Haematology University of Cambridge Cambridge UK University of Leuven Leuven Belgium Broad Institute of MIT and Harvard Cambridge MA USA Center for Cancer Research Massachusetts General Hospital Charlestown MA USA Department of Pathology Massachusetts General Hospital Boston MA USA Harvard Medical School Boston MA USA Center for Cancer Research Massachusetts General Hospital Boston MA USA Dana-Farber Cancer Institute Boston MA USA Department of Medical Oncology Dana-Farber Cancer Institute Boston MA USA Department of Cancer Biology Dana-Farber Cancer Institute Boston MA USA Oxford NIHR Biomedical Research Centre Oxford UK Oxford NIHR Biomedical Research Centre University of Oxford Oxford UK University of Toronto Toronto Ontario Canada Vector Institute Toronto Ontario Canada Vector Institute Toronto ON Canada Department of Computer Science University of Toronto Toronto ON Canada Ontario Institute for Cancer Research Toronto Ontario Canada University of California Los Angeles

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Changing life expectancy in European countries 1990–2021: a subanalysis of causes and risk factors from the Global Burden of Disease Study 2021

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The Lancet Public Health 2025年第3期10卷 e172-e188页

作者： Steel, Nicholas Bauer-Staeb, Clarissa Maria Mercedes Ford, John A. Abbafati, Cristiana Abdalla, Mohammed Altigani Abdelkader, Atef Abdi, Parsa Zuñiga, Roberto Ariel Abeldaño Abiodun, Olugbenga Olusola Abolhassani, Hassan Abu-Gharbieh, Eman Abukhadijah, Hana J. Abu-Zaid, Ahmed Addo, Isaac Yeboah Addolorato, Giovanni Adekanmbi, Victor Adetunji, Juliana Bunmi Adeyeoluwa, Temitayo Esther Agardh, Emilie E. Agyemang-Duah, Williams Ahmad, Danish Ahmed, Ayman Ahmed, Syed Anees Akinosoglou, Karolina Akkaif, Mohammed Ahmed Al Awaidy, Salah Al Hasan, Syed Mahfuz Al Zaabi, Omar Ali Mohammed Aldridge, Robert W. Algammal, Abdelazeem M. Al-Gheethi, Adel Ali Saeed Ali, Abid Ali, Mohammed Usman Ali, Syed Shujait Ali, Waad Alicandro, Gianfranco Alif, Sheikh Mohammad Al-Jumaily, Adel Allebeck, Peter Alrawashdeh, Ahmad Al-Rifai, Rami H. Alsabri, Mohammed A. Alshahrani, Najim Z. Aluh, Deborah Oyine Al-Wardat, Mohammad Al-Zyoud, Walid A. Amiri, Sohrab Anderlini, Deanna Andrei, Catalina Liliana Anil, Abhishek Anvari, Saeid Anyasodor, Anayochukwu Edward Appiah, Seth Christopher Yaw Aquilano, Michele Arabloo, Jalal Arafat, Mosab Areda, Demelash Aremu, Abdulfatai Armani, Keivan Armocida, Benedetta Ärnlöv, Johan Asaduzzaman, Muhammad Astell-Burt, Thomas Aujayeb, Avinash Ausloos, Marcel Azadnajafabad, Sina Aziz, Shahkaar Azzam, Ahmed Y. Babu, Giridhara Rathnaiah Badache, Andreea Corina Badiye, Ashish D. Bahramian, Saeed Baig, Atif Amin Baker, Jennifer L. Bansal, Hansi Bärnighausen, Till Winfried Barone, Mark Thomaz Ugliara Barrow, Amadou Barteit, Sandra Bashir, Shahid Bashiru, Hameed Akande Basso, João Diogo Bastan, Mohammad-Mahdi Basu, Sanjay Batra, Kavita Bauckneht, Matteo Baune, Bernhard T. Beghi, Massimiliano Beiranvand, Maryam Béjot, Yannick Bell, Michelle L. Bello, Olorunjuwon Omolaja Belo, Luis Beloukas, Apostolos Beneke, Alice A. Bettencourt, Paulo J.G. Bhagavathula, Akshaya Srikanth Bhala, Neeraj Bhaskar, Sonu Bisulli, Francesca Bjørge, Tone Bodunrin, Aadam Olalekan Carvajal, Alejandro Botero Bouaoud, Souad Brayne, Carol Brenner, H Department of Primary Care and Public Health University of East Anglia Norwich United Kingdom Office for Health Improvement and Disparities Department of Health and Social Care London United Kingdom Department of Legal and Economic Studies La Sapienza University Rome Italy Rome Italy Hull York Medical School University of Hull Hull United Kingdom Department of Mathematics and Sciences Ajman University Ajman United Arab Emirates Department of Medicine Memorial University St. John's NL Canada Postgraduate Department University of Sierra Sur Miahuatlan de Porfirio Diaz Mexico Yhteiskuntadatatieteen keskus (Centre for Social Data Science) Helsinki Finland Department of Internal Medicine Federal Medical Centre Abuja Nigeria Research Center for Immunodeficiencies Tehran University of Medical Sciences Tehran Iran Department of Medical Biochemistry and Biophysics Karolinska Institutet (Karolinska Institute) Stockholm Sweden Department of Clinical Sciences University of Sharjah Sharjah United Arab Emirates Department of Biopharmaceutics and Clinical Pharmacy University of Jordan Amman Jordan Medical Research Center Hamad Medical Corporation Doha Qatar Department of Biochemistry and Molecular Medicine Alfaisal University Riyadh Saudi Arabia College of Graduate Health Sciences University of Tennessee Memphis TN United States School of Medicine Sydney NSW Australia Centre for Social Research in Health University of New South Wales Sydney NSW Australia Internal Medicine and Alcohol Related Disease Unit Fondazione Policlinico Universitario A. Gemelli IRCCS Rome Italy Department of Medical and Surgical Sciences Università Cattolica di Roma (Catholic University of Rome) Rome Italy Department of Obstetrics and Gynecology University of Texas Medical Branch Galveston TX United States Department of Biochemistry Osun State University Osogbo Nigeria Department of Pharmacology and Therapeutics University of Medical Sciences Ondo Ondo Nigeria Department of Veteri

Background: Decades of steady improvements in life expectancy in Europe slowed down from around 2011, well before the COVID-19 pandemic, for reasons which remain disputed. We aimed to assess how changes in risk factors and cause-specific death rates in different European countries related to changes in life expectancy in those countries before and during the COVID-19 pandemic. Methods: We used data and methods from the Global Burden of Diseases, Injuries, and Risk Factors Study 2021 to compare changes in life expectancy at birth, causes of death, and population exposure to risk factors in 16 European Economic Area countries (Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, the Netherlands, Norway, Portugal, Spain, and Sweden) and the four UK nations (England, Northern Ireland, Scotland, and Wales) for three time periods: 1990–2011, 2011–19, and 2019–21. Changes in life expectancy and causes of death were estimated with an established life expectancy cause-specific decomposition method, and compared with summary exposure values of risk factors for the major causes of death influencing life expectancy. Findings: All countries showed mean annual improvements in life expectancy in both 1990–2011 (overall mean 0·23 years [95% uncertainty interval [UI] 0·23 to 0·24]) and 2011–19 (overall mean 0·15 years [0·13 to 0·16]). The rate of improvement was lower in 2011–19 than in 1990–2011 in all countries except for Norway, where the mean annual increase in life expectancy rose from 0·21 years (95% UI 0·20 to 0·22) in 1990–2011 to 0·23 years (0·21 to 0·26) in 2011–19 (difference of 0·03 years). In other countries, the difference in mean annual improvement between these periods ranged from –0·01 years in Iceland (0·19 years [95% UI 0·16 to 0·21] vs 0·18 years [0·09 to 0·26]), to –0·18 years in England (0·25 years [0·24 to 0·25] vs 0·07 years [0·06 to 0·08]). In 2019–21, there was an overall decrease in mean annual life expectancy a

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Guidelines and definitions for research on epithelial-mesenchymal transition (vol 21, pg 341, 2020)

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NATURE REVIEWS MOLECULAR CELL BIOLOGY 2021年第12期22卷 834-834页

作者： Yang, Jing Antin, Parker Berx, Geert Blanpain, Cedric Brabletz, Thomas Bronner, Marianne Campbell, Kyra Cano, Amparo Casanova, Jordi Christofori, Gerhard Dedhar, Shoukat Derynck, Rik Ford, Heide L. Fuxe, Jonas Garcia de Herreros, Antonio Goodall, Gregory J. Hadjantonakis, Anna-Katerina Huang, Ruby Y. J. Kalcheim, Chaya Kalluri, Raghu Kang, Yibin Khew-Goodall, Yeesim Levine, Herbert Liu, Jinsong Longmore, Gregory D. Mani, Sendurai A. Massague, Joan Mayor, Roberto McClay, David Mostov, Keith E. Newgreen, Donald F. Nieto, M. Angela Puisieux, Alain Runyan, Raymond Savagner, Pierre Stanger, Ben Stemmler, Marc P. Takahashi, Yoshiko Takeichi, Masatoshi Theveneau, Eric Thiery, Jean Paul Thompson, Erik W. Weinberg, Robert A. Williams, Elizabeth D. Xing, Jianhua Zhou, Binhua P. Sheng, Guojun Departments of Pharmacology and Pediatrics Moores Cancer Center University of California San Diego La Jolla CA USA Department of Cellular and Molecular Medicine University of Arizona Tucson AZ USA Molecular and Cellular Oncology Lab Department of Biomedical Molecular Biology Ghent University Cancer Research Institute Ghent (CRIG) VIB Center for Inflammation Research Ghent Belgium Laboratory of Stem Cells and Cancer Université Libre de Bruxelles Bruxelles Belgium Department of Experimental Medicine 1 Nikolaus-Fiebiger-Center for Molecular Medicine Friedrich-Alexander-University Erlangen-Nürnberg Erlangen Germany Division of Biology and Biological Engineering California Institute of Technology Pasadena CA USA Department of Biomedical Science and Bateson Centre University of Sheffield Sheffield UK Departamento de Bioquímica Universidad Autónoma de Madrid (UAM) Instituto de Investigaciones Biomédicas ‘Alberto Sols’ (CSIC-UAM) IdiPAZ & Centro de Investigación Biomédica en Red de Cáncer (CIBERONC) Madrid Spain Institute for Research in Biomedicine (IRB Barcelona) Barcelona Institute of Science and Technology/Institut de Biologia Molecular de Barcelona (IBMB-CSIC) Barcelona Spain Department of Biomedicine University of Basel Basel Switzerland Department of Biochemistry and Molecular Biology University of British Columbia and British Columbia Cancer Research Centre Vancouver BC Canada Departments of Cell and Tissue Biology and Anatomy University of California at San Francisco San Francisco CA USA Department of Pharmacology University of Colorado Anschutz Medical Campus Aurora CO USA Department of Laboratory Medicine (LABMED) Division of Pathology Karolinska University Hospital and Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet Stockholm Sweden Programa de Recerca en Càncer Institut Hospital del Mar d’Investigacions Mèdiques (IMIM) and Departament de Ciències Experimentals i de la Salut Universitat Pompeu Fabra Barcelona Spain C

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Nanofiltration of extracellular vesicles from human plasma & their on-chip qualification and quantification with a NanoBioAnalytical platform

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Meta Gene 2018年 17卷 S7-S7页

作者： Obeid Sameh Benoît Le Roy de Boiseaumarie Celine Elie-Caille Wilfrid Boireau Ming Li Chou Thierry Burnouf Pei-Shan Sung Shie-Liang Hsieh Institut FEMTO-ST Univ. Bourgogne Franche Comté CNRS France Graduate Institute of Biomedical Material and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taiwan International PhD Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taiwan Institute of Clinical Medicine National Yang Ming University Taipei Taiwan Genomic Research Center Academia Sinica Taiwan Department of Medical Research Taipei Veterans General Hospital Taiwan

One complication of plasma transfusion is non-immune transfusion related acute lung injury (TRALI), which is associated with the formation of neutrophil extracellular traps (NETs) resulting from polymorphonuclear neutrophil (PMN) activation. Activated platelets have recently been shown to trigger NETs formation. We hypothesized that (a) NETs could be promoted by platelet derived microparticles (PMPs) and (b) that 75 nm-nanofiltration of plasma, by removing parts of PMPs, could avoid NETs formation. To better characterize the large spectrum of size and concentration of PMPs in complex media, we combined conventional approaches (Flow Cytometry, TRPS) with an original nanobioanalytical (NBA) platform based on Surface Plasmon Resonance imaging (SPRi) and Atomic Force Microscopy (AFM) techniques. We will demonstrate that this new analytic solution allows: • a label-free follow-up of PMPs capture in real time from various complex media, • a multiplex detection of different PMPs subpopulations, • the determination of the size and morphology of captured PMPs in a range of 20 nm to 1 μm their proteomic studies. We expect that such platform will contribute to (i) a better understanding of the physiological function of Evs subpopulations, (ii) the discovery of specific biomarkers and (iii) to therapeutic follow-up.

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Guanylate-binding protein 1(GBP1) contributes to the immunity of human mesenchymal stromal cells against Toxoplasma gondii

Guanylate-binding protein 1(GBP1) contributes to the immunit...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Aiping Qin De-Hua Lai Qifa Liu Weijun Huang Geoff Hide Zhao-Rong Lun Francisco J.Ayala Andy Peng Xiang Department of Hematology Nanfang HospitalSouthern Medical University Center for Stem Cell Biology and Tissue Engineering The Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationZhongshan School of MedicineSun Yat-Sen University Center for Parasitic Organisms State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-Sen University Program of Stem Cells and Regenerative Medicine Affiliated Guangzhou Women and Children’s HospitalZhongshan School of MedicineSun Yat-Sen University Ecosystems and Environment Research Centre School of Environment and Life SciencesUniversity of Salford Biomedical Research Centre School of Environment and Life SciencesUniversity of Salford Department of Ecology and Evolutionary Biology University of California Department of Biochemistry Zhongshan School of MedicineSun Yat-Sen University

Mesenchymal stromal cells(MSCs) have recently been shown to play important roles in mammalian host defenses against intracellular pathogens,but the molecular mechanism still needs to be *** confirmed that human MSCs(hMSCs) prestimulated with IFN-γ showed a significant and dose-dependent ability to inhibit the growth of two types of Toxoplasma gondii [type I RH strain with green fluorescent proteins(RH/GFP) or type II PLK strain with red fluorescent proteins(PLK/RED)].However,in contrast to previous reports,the *** activity of hMSCs was not mediated by indoleamine 2,3-dioxygenase(IDO).Genome-wide RNA sequencing(RNA-seq) analysis revealed that IFN-γ increased the expression of the p65 family of human guanylate-binding proteins(h GBPs) in hMSCs,especially *** analyze the functional role of h GBPs,stable knockdowns of h GBP1,-2,and-5 in hMSCs were established using a lentiviral transfection ***1 knockdown in hMSCs resulted in a significant loss of the *** host defense property,compared with hMSCs infected with nontargeted control sequences.h BP2 and-5 knockdowns had no ***,the h GBP1 accumulation on the parasitophorous vacuole(PV) membranes of IFN-γ–stimulated hMSCs might protect against *** *** together,our results suggest that h GBP1 plays a pivotal role in *** protection of hMSCs and may shed new light on clarifying the mechanism of host defense properties of hMSCs.

关键词： human stem cells parasitic protozoan innate immunity in vitro cultivation

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TALEN-based generation of a cynomolgus monkey disease model for human microcephaly

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细胞研究（英文版） 2016年第9期26卷 1048-1061页

作者： Qiong Ke Weiqiang Li Xingqiang Lai Hong Chen Lihua Huang Zhuang Kang Kai Li Program of Stem Cells and Regenerative Medicine Affiliated Guangzhou Women and Children's HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510623China Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-Sen UniversityGuangzhou 510080China Department of Biology Zhongshan Medical SchoolSun Yat-Sen UniversityGuangzhou 510080China Guangdong Key Laboratory of Reproductive Medicine Guangzhou 510080China Program of Stem Cells and Regenerative Medicine Affiliated Guangzhou Women and Children's HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510623China Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-Sen UniversityGuangzhou 510080China Guangdong Key Laboratory of Reproductive Medicine Guangzhou 510080China Department of Biochemistry Zhongshan Medical SchoolSun Yat-Sen UniversityGuangzhou 510080China Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-Sen UniversityGuangzhou 510080China Program of Stem Cells and Regenerative Medicine Affiliated Guangzhou Women and Children's HospitalZhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510623China Department of Radiology the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510632China Department of Ultrasound the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510632China

Gene editing in non-human primates may lead to valuable models for exploring the etiologies and therapeutic strategies of genetically based neurological disorders in humans. However, a monkey model of neurological disorders that closely mimics pathological and behavioral deficits in humans has not yet been successfully generated. Microcephalin 1 (MCPH1) is implicated in the evolution of the human brain, and MCPH1 mutation causes microcephaly accompanied by mental retardation. Here we generated a cynomolgus monkey (Macaca fascicularis) carrying biallelic MCPH1 mutations using transcription activator-like effector nucleases. The monkey recapitulated most of the important clinical features observed in patients, including marked reductions in head circumference, premature chromosome condensation (PCC), hypoplasia of the corpus callosum and upper limb spasticity. Moreover, overexpression of MCPH1 in mutated dermal fibroblasts rescued the PCC syndrome. This monkey model may help us elucidate the role of MCPH1 in the pathogenesis of human microcephaly and better understand the function of this protein in the evolution of primate brain size.

关键词： MCPH1 microcephaly TALEN gene mutant cynomolgus monkey

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The micro/nanohybrid structures enhancing B35 cell guidance on chitosan

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Journal of Nanotechnology in engineering and medicine 2015年第3期6卷 031005页

作者： Lin, Ying-Ting Li, Ching-Wen Wang, Gou-Jen Graduate Institute of Biomedical Engineering National Chung-Hsing University 250 Kuo Kuang Road Taichung40227 Taiwan Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung-Hsing University 250 Kuo Kuang Road Taichung40227 Taiwan Department of Mechanical Engineering Graduate Institute of Biomedical Engineering National Chung-Hsing University 250 Kuo Kuang Road Taichung40227 Taiwan

A novel chitosan scaffold with micro- and nano-hybrid structures was proposed in this study. The hemispheric array of the barrier layer of an anodic aluminum oxide (AAO) film was used as the substrate. Microelectromechanical systems and nickel electroforming techniques were integrated for fabricating chitosan scaffolds with different micro/ nanohybrid structures. Nerve cells were then cultured on the conduits. It was demonstrated that the scaffold with pure microstructures can guide the nerve cells to grow along the ridges of the microstructure and some cells to grow across the groove in between two ridges of the microstructure. It was also shown that the scaffold with microscale ridges and nanopatterns on the groove between two ridges can more effectively guide the cells to grow along the ridges, thus enhancing the proliferation of nerve cells. © 2016 by ASME.

关键词： Chitosan

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