检索结果-内蒙古大学图书馆

Pharmacological inhibition of carbonic anhydrases ameliorates cognitive dysfunction, rescuing Aβ-induced neurovascular pathology in vivo

引用

Alzheimer's & dementia : the journal of the Alzheimer's Association 2021年第S3期17卷 e054199-e054199页

作者： Elisa Canepa Rafael Vazquez-Torres Rebecca M Parodi-Rullan Federica Angiulli Ludovic Debure Silvia Fossati Temple University PA Philadelphia United States PhD Program in Neuroscience University of Milano - Bicocca Monza Italy NYU Grossman School of Medicine NY NY United States

BACKGROUND: Cerebrovascular dysfunction (CVD) is an early feature of Alzheimer's disease (AD), contributing to the pathology progression, and suggesting a strict association between CVD and neurodegeneration. The majority of AD cases present cerebral amyloid angiopathy (CAA), neuropathological feature characterized by abnormal vasculotropic deposition of amyloid beta, mainly Aβ40. Severe CAA is also induced by familial Aβ variants, such as the Dutch-Q22. Our previous in vitro studies demonstrated that brain vascular amyloidosis elicits mitochondrial dysregulation and caspase-mediated apoptosis, in cells composing the neurovascular unit, including neurons, endothelial, glial and smooth muscle cells. Additionally, we showed that acetazolamide (ATZ) and methazolamide (MTZ), FDA-approved carbonic anhydrase inhibitors (CAIs) which cross the blood-brain barrier (BBB), hamper these detrimental processes. Carbonic anhydrases (CAs) represent a family of metalloenzymes catalyzing the reversible hydration of carbon dioxide, and their inhibition improves cerebral blood flow, vasoreactivity and neuronal excitability, pointing to CAs as CVD targets in AD. METHOD: We employed Tg-SwDI mice (expressing human amyloid precursor protein, APP, carrying the Swedish, Dutch and Iowa mutations), which develop fibrillar amyloid burden, primarily in the cerebral microvasculature, starting at 6 months. We fed the animals (from 8 to 16 months of age) a CAI-diet, following which we performed behavioral analysis, and harvested the brains for both biochemical and immunohistochemical examination. RESULT: Compared to untreated Tg mice, ATZ- and MTZ-fed animals showed reduced cognitive impairment, along with decreased vascular Aβ overload and astrogliosis, main pathological hallmarks of the disease. Tg animals exhibited Aβ deposition in endothelial and glial cells, leading to cell-specific caspase-3 activation. Interestingly, CAI-diet reduced endothelial and astrocytic Aβ deposits, and Aβ-induced cas

关键词：

来源：评论

学校读者我要写书评

暂无评论

Reply to "Understanding the sources of cortico-cortical paired associative stimulation (ccPAS) variability: Unraveling target-specific and state-dependent influences"

引用

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology 2023年 156卷 293-294页

作者： Arantzazu San Agustín Domenica Veniero Jose L Pons Julio C Hernandez-Pavon PhD Program in Neuroscience Autonoma de Madrid University-Cajal Institute Madrid 28029 Spain. Electronic address: arantzazusanagustinperez@***. School of Psychology University of Nottingham Nottingham UK. Legs + Walking Lab Shirley Ryan AbilityLab (Formerly The Rehabilitation Institute of Chicago) Chicago IL USA Department of Physical Medicine and Rehabilitation Feinberg School of Medicine Northwestern University Chicago IL USA. Department of Psychological Sciences Kansas State University USA. Electronic address: juliohpavon@ksu.edu.

来源：评论

学校读者我要写书评

暂无评论

Social environment affects the vulnerability to adolescent-onset depression and determines antidepressant efficacy in a preclinical model

引用

neuroscience Applied 2023年 2卷

作者： N. Ciano Albanese A. Reccagni C. Barezzi F. Marsili S. Poggini I. Branchi Istituto Superiore di Sanità Center for Behavioral Sciences and Mental Health Rome Italy Sapienza University of Rome PhD program in Clinical-experimental Neuroscience and Psychiatry Rome Italy

来源：评论

学校读者我要写书评

暂无评论

Exploring sex-dependent ketamine rapid behavioral effects in a mouse model of chronic restraint stress

引用

neuroscience Applied 2024年 3卷

作者： N. Nicosia J. Mingardi P. Misztak M. Giovenzana L. Musazzi PhD Program in Neuroscience- University of Milano-Bicocca School of Medicine and Surgery Monza Italy University of Milano-Bicocca School of Medicine and Surgery Monza Italy Fondazione IRCCS San Gerardo dei Tintori Monza Italy

来源：评论

学校读者我要写书评

暂无评论

Translational models of stress and resilience: An applied neuroscience methodology review

引用

neuroscience Applied 2024年 3卷

作者： Albayrak, Zeynep Seda Vaz, Andreia Bordes, Joeri Ünlü, Selen Sep, Milou S.C. Vinkers, Christiaan H. Pinto, Luisa Yapici-Eser, Hale Koç University Hospital Department of Child and Adolescent Psychiatry Istanbul Turkey Koç University Graduate School of Health Sciences Neuroscience PhD Program Koç University Istanbul Turkey Life and Health Sciences Research Institute (ICVS) School of Medicine University of Minho Campus de Gualtar Braga 4710-057 Portugal ICVS/3B's–PT Government Associate Laboratory Braga 4710-057 Portugal Bn'ML Behavioral and Molecular Lab Campus de Gualtar Braga 4710-057 Portugal Neurocentre Magendie INSERM 1215 Université de Bordeaux Bordeaux France Koç University School of Medicine İstanbul Turkey Department of Psychiatry Amsterdam University Medical Centers Location Vrije Universiteit Amsterdam Netherlands GGZ InGeest Mental Health Care Amsterdam Netherlands Amsterdam Neuroscience Mood Anxiety Psychosis Sleep & Stress Program Amsterdam Netherlands Amsterdam Public Health Mental Health Program Amsterdam Netherlands Department of Psychiatry and Department of Anatomy and Neurosciences Amsterdam University Medical Center Location VUMC Amsterdam Netherlands Academic Working Place Depression GGZ InGeest Amsterdam Netherlands Amsterdam Neuroscience (Mood Anxiety Psychosis Stress & Sleep Program) and Amsterdam Public Health (Mental Health Program) Research Institutes Amsterdam Netherlands Koç University School of Medicine Department of Psychiatry İstanbul Turkey

Stress, encompassing psychological, physical, and physiological challenges, is an important factor affecting an individual's well-being and potentially leading to psychiatric, neurodegenerative, immune, and metabolic disorders. However, not everyone exposed to stress develops these conditions, highlighting the concept of resilience. Resilience is a dynamic process categorized into four dimensions: pre-existing resilience capacity, ongoing resilience processes, post-stress resilience outcomes, and recovery from psychopathologies. These dimensions involve genomic, cellular, and systemic interactions influenced by genetic factors, early life experiences, adult life experiences in addition to community/environmental factors, and health behaviors. The biological response to stress encompasses endocrine, autonomic, immunological, and behavioral components, modulated by stressor characteristics and individual traits. Due to the limitations in studying stress and resilience in humans, translational models using rodents and cell cultures are essential. Rodent models include acute, chronic, and traumatic stress paradigms, aiding the study of stress-related behavioral and molecular outcomes. Additionally, early life stress models, such as prenatal stress and maternal separation, provide insights into developmental impacts. In this review, first, rodent models for lifelong stress exposure will be summarized considering their validity, advantages, and limitations. Subsequently, an overview of models designed to enhance resilience capacity and process in rodents, and later the behavioral models employed to study the outcomes of resilience will be given. Lastly, the focus will be shifted to cell culture and iPSCs models. Finally, future considerations focused on improving translational models used to study stress and resilience will be discussed. It is aimed to provide an overview of designs for translational stress and resilience models to access more effective translational biom

关键词： Acute stress Behavior Chronic stress Resilience Rodent Stress

来源：评论

学校读者我要写书评

暂无评论

Brain structural correlates of treatment resistance in major depressive disorder and their evolution over time

引用

neuroscience Applied 2024年 3卷

作者： M. Maccario M. Paolini S. Poletti L. Raffaelli R. Zanardi C. Colombo F. Benedetti IRCCS Scientific Institute Ospedale San Raffaele Psychiatry & Clinical Psychobiology- Division of Neuroscience Milano Italy Vita-Salute San Raffaele University UniSR Residency Program- Psychiatry Milan Italy IRCCS Scientific Institute Ospedale San Raffaele Psychiatry & Clinical Psychobiology- Division of Neuroscience Milan Italy Vita-Salute San Raffaele University Division of Neuroscience Milan Italy Vita-Salute San Raffaele University PhD Program in Cognitive Neuroscience- Division of Neuroscience Milan Italy IRCCS Scientific Institute Ospedale San Raffaele Mood Disorders- San Raffaele Turro Milan Italy

来源：评论

学校读者我要写书评

暂无评论

Social isolation in adolescence leads to different cognitive and emotional dysfunctions in males and females: a preclinical study

引用

neuroscience Applied 2022年 1卷

作者： N. Ciano Albanese A. Reccagni C. Barezzi S. Poggini I. Branchi Istituto Superiore di Sanità Center for Behavioral Sciences and Mental Health Rome Italy Sapienza University of Rome PhD program in Behavioral Neurosciences Rome Italy Sapienza University of Rome PhD program in Clinical-experimental Neuroscience and Psychiatry Rome Italy

来源：评论

学校读者我要写书评

暂无评论

Protocol for a Wnt reporter assay to measure its activity in human neural stem cells derived from induced pluripotent stem cells

引用

Current Research in Neurobiology 2023年 5卷 100095页

作者： Yde Ohki, Cristine Marie Walter, Natalie Monet Rickli, Michelle Salazar Campos, José Maria Werling, Anna Maria Döring, Christian Walitza, Susanne Grünblatt, Edna Department of Child and Adolescent Psychiatry and Psychotherapy Translational Molecular Psychiatry Psychiatric University Hospital Zurich University of Zurich Wagistrasse 12 Schlieren 8952 Switzerland Neuroscience Center Zurich University of Zurich and the ETH Zurich Winterthurerstrasse 11 Zurich 8057 Switzerland Zurich Center for Integrative Human Physiology University of Zurich Winterthurerstrasse 11 Zurich 8057 Switzerland Biomedicine PhD Program University of Zurich Winterthurerstrasse 11 Zurich 8057 Switzerland

The canonical Wnt signaling is an essential pathway that regulates cellular proliferation, maturation, and differentiation during neurodevelopment and maintenance of adult tissue homeostasis. This pathway has been implicated with the pathophysiology of neuropsychiatric disorders and was associated with cognitive processes, such as learning and memory. However, the molecular investigation of the Wnt signaling in functional human neural cell lines might be challenging since brain biopsies are not possible and animal models may not represent the polygenic profile of some neurological and neurodevelopmental disorders. In this context, using induced pluripotent stem cells (iPSCs) has become a powerful tool to model disorders that affect the Central Nervous System (CNS) in vitro, by maintaining patients’ genetic backgrounds. In this method paper, we report the development of a virus-free Wnt reporter assay in neural stem cells (NSCs) derived from human iPSCs from two healthy individuals, by using a vector containing a reporter gene (luc2P) under the control of a TCF/LEF (T-cell factor/lymphoid enhancer factor) responsive element. Dose-response curve analysis from this luciferase-based method might be useful when testing the activity of the Wnt signaling pathway after agonists (e.g. Wnt3a) or antagonists (e.g. DKK1) administration, comparing activity between cases and controls in distinct disorders. Using such a reporter assay method may help to elucidate whether neurological or neurodevelopmental mental disorders show alterations in this pathway, and testing whether targeted treatment may reverse these. Therefore, our established assay aims to help researchers on the functional and molecular investigation of the Wnt pathway in patient-specific cell types comprising several neuropsychiatric disorders. © 2023 The Authors

关键词： Human stem cells Induced pluripotent stem cells Neural stem cells Neuropsychiatry Reporter assay Wnt signaling

来源：评论

学校读者我要写书评

暂无评论

Crosstalk across barriers: choroid plexus volume affects white matter integrity in bipolar depression

引用

neuroscience Applied 2023年 2卷

作者： B. Bravi E.M.T. Melloni M. Paolini M. Biondi C. Colombo R. Zanardi F. Benedetti IRCCS Scientific Institute Ospedale San Raffaele Psychiatry & Clinical Psychobiology Unit- Division of Neuroscience Milan Italy University Vita-Salute San Raffaele Psychology- PhD program in Cognitive Neurosciences Milan Italy University Vita-Salute San Raffaele Neuroscience- PhD program in Molecular Medicine Milan Italy Vita-Salute San Raffaele University Neuropsychiatric Sciences Milan Italy IRCCS Ospedale San Raffaele Department of Affective Disorders- Mood Disorders Unit Milan Italy

来源：评论

学校读者我要写书评

暂无评论

Fabrication of hyaluronic acid with graphene quantum dot as a dual drug delivery system for cancer therapy

引用

FlatChem 2024年 44卷

作者： Lin, Joseph Lin, Jung-Hua Yeh, Tseng-Yu Zheng, Jia-Huei Cho, Er-Chieh Lee, Kuen-Chan Department of Science Education National Taipei University of Education No.134 Sec. 2 Heping E. Rd. Da-an District Taipei City 106 Taiwan School of Pharmacy College of Pharmacy Taipei Medical University 250 Wuxing Street Taipei City 110 Taiwan Master Program in Clinical Genomics and Proteomics College of Pharmacy Taipei Medical University 250 Wuxing Street Taipei City 110 Taiwan Cancer Center Wan Fang Hospital Taipei Medical University 110 Taiwan PhD Program in Medical Neuroscience College of Medical Science and Technology Taipei Medical University Taipei City 110 Taiwan

With recent advancements in nanomedicine, there has been growing interest in developing drug delivery systems with multifunctional capabilities. In this study, we developed a novel dual drug delivery system by combining two drug carriers via positive and negative electrostatic interactions. First, we modified polyethyleneimine (PEI) with graphene quantum dots (GQDs) to create a positively charged particle (GPI) with high drug loading efficiency and good dispersibility. Second, we embedded the pyrenebutyric acid structure in hyaluronic acid (HA) through EDC/NHS cross-linking and used TAK-632 as the hydrophobic drug to create negatively charged particles (HANPs) with hydrogel-like properties and the CD44 receptor. After the two components were constructed, the increase in the particle charge and simultaneous delivery of both drugs synergistically enhanced the therapeutic effect of this strategy. Qualitative tests confirmed the successful synthesis of the drug carrier, while the potential of this system as a cancer treatment strategy was evaluated in HCT116 cancer cells (through MTT cell viability assays) and in vivo mice xenograft experiments. Our results demonstrated that the dual drug delivery system, HANPs(TAK)/GPI(DOX), had a significant inhibitory effect on the growth of cancer cells both in vitro and in vivo, suggesting that this system is a promising candidate for a new type of treatment. © 2024 Elsevier B.V.

关键词： Dual drug delivery system Electrostatic interaction Graphene quantum dots Hydrophobic drug

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：