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Multivariate analysis and image-based phenotyping of cayenne fruit traits in selection and diversity mapping of multiple F1 cross lines

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Reproduction and Breeding 2024年第4期4卷 194-202页

作者： Anshori, Muhammad Fuad Musa, Yunus Dungga, Novaty Eny Widiayani, Nuniek Arifin, Arfina Sukmawati Masniawati, A. Firmansyah Farid, Muh Dirpan, Andi Sakinah, Andi Isti Amier, Nirwansyah Department of Agronomy Faculty of Agriculture Hasanuddin University Makassar 90245 Indonesia Department of Agricultural Technology Faculty of Agriculture Hasanuddin University Makassar 90245 Indonesia Department of Biology Faculty of Mathematics and Natural Science Hasanuddin University Makassar 90245 Indonesia Research Center for Food Crops Research Organization for Agriculture and Food National Research and Innovation Agency (BRIN) Cibinong Science Center Bogor 16911 Indonesia Graduate School Hasanuddin University Makassar 90245 Indonesia Agrotechnology Study Program Graduate School Hasanuddin University Makassar 90245 Indonesia

The phenomenon of fluctuating chili prices can be resolved in stages, one of which is through multiple crosses. However, this cross requires precise methods in the evaluation and selection process, especially regarding fruit characteristics. Image-based phenotyping 4.0 approaches can increase the potential precision of such evaluation genotypes, especially when this approach is combined with multivariate analysis. Therefore, both methods are needed to evaluate and select these cayenne multiple crosses. This research aims to identify the effectiveness of multivariate analysis and image-based explanatory characteristics of fruit phenotypes and to select multiple crosses that can continue to the F2 generation. The research was designed with a randomized complete block design of ten F1 multiple cross-genotypes and four check varieties. Each genotype was repeated three times, so there were 42 experimental units. Based on the results, multivariate was considered adequate in determining image explanatory characters based on fruit phenotype and genotype mapping of the population diversity of multiple crosses of cayenne pepper. The characteristics of fruit height, fruit area, and fruit Intden are image-based explanatory characters that can map the completeness of cayenne pepper fruit between multiple crosses well. This indicates that image-based phenotyping and multivariate analysis can provide more detailed image information of the potential of cayenne fruit from multiple crosses than just based on fruit weight. Therefore, both approaches are recommended for analyzing cayenne paper fruit potential, especially for multiple crosses. In addition, three crosses (MC4, MC8, and MC9) are optimal for the next generation to be recommended and continued. © 2024 The Authors

关键词： 3D plot Capsicum frutescent Cayenne breeding Image processing Multiple cross

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Polyelectrolyte nanoparticles derived from durian seed gum modified with chitosan for selective targeting in cancer cells

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International Journal of Biological Macromolecules 2025年 315卷 144625页

作者： Jindrayani Nyoo Putro Fasih Bintang Ilhami Chih-Chia Cheng Keziah Janice Imanuela Dave Mangindaan Wenny Irawaty Astrid Rahmawati Osamu Shimomura Suryadi Ismadji Chemical Engineering Master Program Faculty of Engineering Widya Mandala Surabaya Catholic University Kalijudan 37 Surabaya 60114 Indonesia Collaborative Research Center for Sustainable and Zero Waste Industries Jl. Kalijudan 37 Surabaya 60114 East Java Indonesia Department of Natural Science Faculty of Mathematics and Natural Science Universitas Negeri Surabaya Surabaya 60231 Indonesia Graduate Institute of Applied Science and Technology National Taiwan University of Science and Technology Taipei 10607 Taiwan Department of Chemical Engineering Widya Mandala Surabaya Catholic University Kalijudan 37 Surabaya 60114 Indonesia Professional Engineer Program Department Faculty of Engineering Bina Nusantara University K.H. Syahdan No. 9 Jakarta 11480 Indonesia Waste-Food-Environmental Nexus Research Interest Group Bina Nusantara University K.H. Syahdan No. 9 Jakarta 11480 Indonesia Department of Applied Chemistry Osaka Institute of Technology 5-16-1 Omiya Ashahi-ku Osaka 535-8585 Japan

The synthesis of natural polysaccharides from durian seed gum-modified chitosan as complex nanoparticles was successfully developed. The complex formation of nanoparticles was confirmed by 1 H NMR, indicating the interpenetrating polymer network between the durian seed gum and chitosan. The material's physical properties were analyzed using ZETA potential, SEM, FTIR, swelling assay, and spectrofluorometer. The material showed a pH-responsive behavior as evidenced by the swelling assay, in which the complex nanoparticle has a smaller swelling index in pH 5.5 and 7.4 than pH 2.1. The stability of the material was tested by checking the hydrodynamic diameter, which resulted in the modification of chitosan having higher kinetic stability than the durian seed gum under a diameter smaller than 1 μm. The chitosan-modified durian seed gum's hemolytic activity indicates blood compatibility with 4 % hemolytic activity under high nanoparticle concentration (400 μg/mL). More importantly, the cytotoxicity test of complex nanoparticles showed biocompatibility with NIH/3 T3 cells, whereas complex nanoparticles exhibited high toxicity towards HeLa cells. Moreover, complex nanoparticles showed strong intracellular blue fluorescence with selective activity towards cancer cells. Hence, this newly developed green synthesis of polyelectrolyte nanoparticles derived from durian seed gum modified with chitosan improved selectivity and intracellular fluorescence in tracking cancer cells.

关键词： Bioimaging Chitosan Cytotoxicity Durian seed gum Polyelectrolyte nanoparticles

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Genetic variation of somaclonal mutants from the 8th generation of Pekalongan accession rodent tuber (typhonium flagelliforme lodd.) based on RAPD-PCR analysis 2

Genetic variation of somaclonal mutants from the 8th generat...

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2nd International Conference on Eco Engineering Development 2018, ICEED 2018

作者： Purnamaningsih, Ragapadmi Sianipar, Nesti Fronika Assidqi, Khoirunnisa Spits Warnars, Harco Leslie Hendric Indonesia Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor16111 Indonesia Food Technology Department Faculty of Engineering Bina Nusantara University Jakarta11480 Indonesia Research Interest Group Food Biotechnology Bina Nusantara University Jakarta11480 Indonesia Computer Science Department BINUS Graduate Program - Doctor of Computer Science Bina Nusantara University Jakarta11480 Indonesia

Rodent tuber is a potential plant which can be developed as an anticancer drug. The rodent tuber plant has a low anticancer compound which does not have an economic value to be utilized on an industrial scale, particularly to explore as an anticancer drug. The material plant was used 30 clones of Rodent Tuber Pekalongan to increase its bioactive compounds using a combination of in vitro culture with gamma-ray irradiation. The selection process has been done until the 8th generation and obtained 14 somaclonal mutants that have higher bioactive compounds than control plant (non-mutant). This study aimed to analyze the genetic variation of 14 somaclonal mutants of rodent tuber using RAPD with 14 primers. The results were produced 513 total bands with the size of 200-5000 bp and 380 polymorphic bands between somaclonal mutants. OPB-18 primer produced the most polymorphic bands and five specific polymorphic bands of 1336 bp, 1070 bp, 901 bp, 861 bp and 728 bp in the somaclonal mutant M23 (20-4-2-1-1-1). OPD-20 primer produced seven specific polymorphic bands of 1574 bp, 1557 bp, 1501 bp, 1496 bp, 1234 bp, 1229 bp and 1086 bp in the M23 somaclonal mutant. The phylogenetic analysis showed that there were two main groups with the coefficient similarity between 0.77 and 0.83. The highest genetic variation obtained was found in the M23 somaclonal mutant with a genetic difference of 23% compared with control. There are five somaclonal mutants (M24, M22, M21, M16, M14) that have a genetic variation of up to 22% over the control. This study shows that OPB-18 and OPD-20 primers were efficient in detecting somaclonal mutant variants. © 2018 Institute of Physics Publishing. All rights reserved.

关键词： Gamma rays

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Chemical Profile and Biological Activity of Clove (Syzygium Aromaticum) Extracts Obtained from Different Extraction Methods

SSRN

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SSRN 2023年

作者： Battestin Quast, Leda da Silva Garais, Bruna Krüger, Roberta Letícia Food Science and Technology Post- Graduation Program Federal University of Southern Frontier Parana Laranjeiras do Sul Brazil Department of Food Engineering Midwest State University Parana Guarapuava Brazil

This study aimed to evaluate the biological activity of clove (Syzygium aromaticum) essential oil and clove extracts, obtained by hydrodistillation, maceration, and ultrasound-assisted extraction. E. coli, L. monocytogenes, S. enteritidis, and S. aureus strains were used to determine the biological activity by the formation of inhibition zones. The chemical profile of the extracts was analyzed in a liquid-phase chromatograph. The ultrasound-assisted essential oils and extracts exhibited biological activity at the concentration of 100 µL/mL, with the highest inhibition halos observed for all strains studied. The essential oil presented greater inhibition for the concentration of 50 µL/mL. The chemical profile analysis demonstrated the presence of groups of gallic acid compounds, flavones, and their isomers. The total phenolics contents ranged from 0.101 and 0.290 mg GAE/g, with the highest values for the ultrasound-assisted extracts. The results of DPPH varied from 8.15 to 65.24%, with an emphasis on ultrasound-assisted essential oil and extracts. © 2023, The Authors. All rights reserved.

关键词： Essential oils

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A first-principles study on the quantum tunneling of methylhydroxycarbene isomerization in various solvents 4

A first-principles study on the quantum tunneling of methylh...

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4th Padjadjaran International Physics Symposium 2019, PIPS 2019

作者： Susanti, E.D. Rusydi, F. Puspitasari, I. Fadilla, R.N. Aisyah, N.D. Ahmad, A. Research Center for Quantum Engineering Design Faculty of Science and Technology Universitas Airlangga Jl. Mulyorejo Surabaya60115 Indonesia Department of Physics Faculty of Science and Technology Universitas Airlangga Jl. Mulyorejo Surabaya60115 Indonesia Precision Sciences and Technology and Applied Physics Graduate School of Engineering Osaka University Suita565-0871 Japan Information System Study Program Faculty of Science and Technology Universitas Airlangga Jl. Mulyorejo Surabaya60115 Indonesia School of Chemical Science and Food Technology Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Selangor43600 Malaysia

Quantum tunneling phenomenon allows a high-barrier reaction to occur even though thermal energy is not sufficient to surmount the barrier. The phenomenon has been suggested to exist in the isomerization of methylhydroxycarbene which occured at 11 K. Here we study the quantum tunneling that occured in the isomerization of methylhydroxycarbene to acetaldehyde and to vinyl alcohol based on density functional calculations. The isomerization pathways are determined under the effect of various solvents which are modeled by polarizable continuum model (PCM). We use Wentzel-Kramers-Brillouin (WKB) approximation to calculate the tunneling probability (T) and extend the usefulness of T into isomerization rate. The results show that the solvents give a significant effect on the isomerization rate of methylhydroxycarbene to acetaldehyde but an insignificant one on the isomerization rate to vinyl alcohol. © 2020 IOP Publishing Ltd. All rights reserved.

关键词： Isomerization

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Anti-inflammatory effect of Momordica grosvenori Swingle extract through suppressed LPS-induced upregulation of iNOS and COX-2 in murine macrophages

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Journal of Functional foods 2009年第2期1卷 145-152页

作者： Pan, Min-Hsiung Yang, Ji-Rui Tsai, Mei-Ling Sang, Shengmin Ho, Chi-Tang Department of Seafood Science National Kaohsiung Marine University Kaohsiung 811 Taiwan Nutrition Research Program University of North Carolina Nutrition Center Julius L. Chambers Biomedical/Biotechnology Research Institute Kannapolis NC 28081 North Carolina Research Campus 500 Laureate Way United States Department of Food Science Rutgers University New Brunswick NJ 08901-8520 United States Graduate Institute of Food Science and Technology National Taiwan University Taipei 100 Taiwan

Momordica grosvenori Swingle, a traditional medicinal herb, is known to possess anti-inflammatory anti-oxidative, anti-diabetic, and nephroprotective activities. Here, we investigated the inhibitory effects of M. grosvenori Swingle extract (MSE) on the induction of NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in murine RAW 264.7 cells activated with lipopolysaccharide (LPS). Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated that MSE significantly blocked protein and mRNA expression of iNOS and COX-2 in LPS-induced macrophages. Treatment with MSE resulted in the reduction of LPS-induced nuclear translocation of nuclear factor-κB (NFκB) subunit and the dependent transcriptional activity of NFκB by blocking phosphorylation of inhibitor κB (IκB)α and p65 and subsequent degradation of IκBα. Transient transfection experiments using NFκB reporter constructs indicated that MSE inhibits the transcriptional activity of NFκB in LPS-stimulated mouse macrophages. MSE also inhibited LPS-induced activation of PI3K/Akt, extracellular signal-regulated kinase 1/2 and p38 MAPK. Taken together, these results show that MSE down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NFκB by interfering with the activation PI3K/Akt/IKK and MAPK. These results have important implication for using MSE towards the development of effective anti-inflammatory agents. Crown Copyright © 2009.

关键词： Cyclooxygenase-2 (COX-2) Inducible NO synthesis (iNOS) Lipopolysaccharide (LPS) Mitogen-activated protein (MAPK) Momordica grosvenori Swingle NFκB Phosphatidylinositol 3-kinase (PI3K) RAW 264.7 monocyte/macrophages

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Utilising Biomass in Biotechnology 1

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丛书名： Green Energy and technology

1000年

作者： Helen Treichel Thamarys Scapini Aline Frumi Camargo Fábio Spitza Stefanski Gislaine Fongaro Bruno Venturin

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An Efficient Three-Factor Authenticated Key Agreement Technique Using FCM Under HC-IoT Architectures

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Computers, Materials & Continua 2022年第7期72卷 1373-1389页

作者： Chandrashekhar Meshram Agbotiname Lucky Imoize Sajjad Shaukat Jamal Parkash Tambare Adel R.Alharbi Iqtadar Hussain Department of Post Graduate Studies and Research in Mathematics Jayawanti Haksar Government Post-Graduation CollegeCollege of Chhindwara UniversityBetul460001M.P.India Department of Electrical and Electronics Engineering Faculty of EngineeringUniversity of LagosAkokaLagos100213Nigeria Department of Electrical Engineering and Information Technology Institute of Digital CommunicationRuhr University44801BochumGermany Department of Mathematics College of ScienceKing Khalid UniversityAbhaSaudi Arabia Water Resources&Applied Mathematics Research Lab Nagpur440027India College of Computing and Information Technology University of TabukTabuk71491Saudi Arabia Mathematics Program Department of MathematicsStatistics and PhysicsCollege of Arts and SciencesQatar University2713DohaQatar

The Human-Centered Internet of Things(HC-IoT)is fast becoming a hotbed of security and privacy *** users can establish a common session key through a trusted server over an open communication channel using a three-party authenticated key *** of the early authenticated key agreement systems relied on pairing,hashing,or modular exponentiation processes that are computationally intensive and *** order to address this problem,this paper offers a new three-party authenticated key agreement technique based on fractional chaotic *** new scheme uses fractional chaotic maps and supports the dynamic sensing of HC-IoT devices in the network architecture without a password *** projected security scheme utilized a hash function,which works well for the resource-limited HC-IoT *** results show that our new technique is resistant to password guessing attacks since it does not use a ***,our approach provides users with comprehensive privacy protection,ensuring that a user forgery attack causes no ***,our new technique offers better security features than the techniques currently available in the literature.

关键词： Three-party authenticated key agreement anonymity fractional chaotic maps Chebyshev polynomial password table human-centered internet of things(HC-IoT)

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Low intensity laser therapy accelerates muscle regeneration in aged rats

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Photonics and Lasers in Medicine 2012年第4期1卷 287-297页

作者： Vatansever, Fatma Rodrigues, Natalia C. Assis, Livia L. Peviani, Sabrina S. Durigan, Joao L. Moreira, Fernando M.A. Hamblin, Michael R. Parizotto, Nivaldo A. Physiotherapy Department Federal University of São Carlos Via Washington Luiz km 235 13565-905 - Sao Carlos SP Brazil Wellman Center for Photomedicine Massachusetts General Hospital Boston MA United States Department of Dermatology Harvard Medical School Boston MA United States Biotechnology Post-Graduate Program Federal University of São Carlos SP Brazil Harvard-MIT Division of Health Science and Technology Cambridge MA United States

Background: Elderly people suffer from skeletal muscle disorders that undermine their daily activity and quality of life;some of these problems can be listed as but not limited to: sarcopenia, changes in central and peripheral nervous system, blood hypoperfusion, regenerative changes contributing to atrophy, and muscle weakness. Determination, proliferation and differentiation of satellite cells in the regenerative process are regulated by specific transcription factors, known as myogenic regulatory factors (MRFs). In the elderly, the activation of MRFs is inefficient which hampers the regenerative process. Recent studies found that low intensity laser therapy (LILT) has a stimulatory effect in the muscle regeneration process. However, the effects of this therapy when associated with aging are still unknown. Objective: This study aimed to evaluate the effects of LILT (λ = 830 nm) on the tibialis anterior (TA) muscle of aged rats. Subjects and methods: The total of 56 male Wistar rats formed two population sets: old and young, with 28 animals in each set. Each of these sets were randomly divided into four groups of young rats (3 months of age) with n = 7 per group and four groups of aged rats (10 months of age) with n = 7 per group. These groups were submitted to cryoinjury + laser irradiation, cryoinjury only, laser irradiation only and the control group (no cryoinjury/no laser irradiation). The laser treatment was performed for 5 consecutive days. The first laser application was done 24 h after the injury (on day 2) and on the seventh day, the TA muscle was dissected and removed under anesthesia. After this the animals were euthanized. Histological analyses with toluidine blue as well as hematoxylin- eosin staining (for counting the blood capillaries) were performed for the lesion areas. In addition, MyoD and VEGF mRNA was assessed by quantitative polymerase chain reaction. Results: The results showed significant elevation (p

关键词： Rats

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Antimicrobial effectiveness of biobased film against Escherichia coli 0157:H7, Listeria monocytogenes and Salmonella typhimurium

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Advance Journal of food science and technology 2011年第4期3卷 294-302页

作者： Theinsathid, Pornpun Visessanguan, Wonnop Kingchaand, Yutthana Keeratipibul, Suwimon Graduate School Interdisciplinary Program Technopreneurship and Innovation Management Chulalongkorn University Bangkok Thailand Thailand Science Park Bangkok Thailand Department of Food Technology Faculty of Science Chulalongkorn University Bangkok Thailand

Antimicrobial packaging, an active packaging concept, can be considered challenging technology that could have a significant impact on food safety of meat and meat products. The feasibility of polylactic acid (PLA)-based film was evaluated for its application as a material for antimicrobial film. A bio-based commercial polylactic acid (PLA) product, Ecovio*, was used as an environmentally friendly polymer matrix. The PLA based film was incorporated with lactic acid or sodium lactate by extrusion film-blowing process. The antimicrobial activity of films against Escherichia coli O157:H7, Listeria monocytogenes and Salmonella entérica Serovar Typhimurium (S. Typhimurium) were evaluated. Antimicrobial film incorporated with lactic acid packaging film was found to be highly effective in inhibiting L. monocytogenes. In contrast, no inhibitory activity was observed against E. coli O157:H7, and S. Typhimurium. This is consistent with Minimum Inhibitory Concentration (MIC) studies which indicated that undissociated lactic acid was more efficient in inhibiting L. monocytogenes than enterobacteria. This preliminary study shows the potential use of bio-based film as one hurdle technology in combination with good manufacturing practices and adequate storage temperatures. The use of antimicrobial packaging may contribute to improve the safety in minimally processed foods. Further work is required to improve the mechanical properties of the material in order to meet industry requirements. © Maxwell Scientific Organization, 2011.

关键词： Lactic acid

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