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SSRN 2023年

作者： Abreu, Thaianaly Leite Estévez, Mario de Carvalho, Leila Moreira de Medeiros, Lorena Lucena da Silva Pereira, Valquíria Cardoso Salu, Bruno Ramos Vilela Oliva, Maria Luiza Madruga, Marta Suely Alencar Bezerra, Taliana Kênia Post-Graduate Program in Food Science and Technology Department of Food Engineering Technology Centre Federal University of Paraiba PB João Pessoa58051-900 Brazil Research Institute of Meat and Meat Products IPROCAR TECAL Research Group Universidad de Extremadura Cáceres10003 Spain Department of Biochemistry Federal University of São Paulo SP São Paulo Brazil

The large amounts of by-products generated in the coffee industry are a problem for a sustainable agro-industry. The aim of this work was to investigate the occurrence of phenolic compounds in organic coffee husks and to evaluate their potential as a source of bioactive dietary components. To achieve this objective, three extracts were prepared, namely extractable polyphenols (EP), hydrolyzable non-extractable polyphenols (HNEP) and non-extractable polyphenols (NEP). These extracts were characterized and evaluated for their bioactive properties after simulated gastrointestinal digestion. The results show that the extraction process affects the occurrence of phenols from coffee peels, especially for caffeic acid, gallic acid, and chlorogenic acid. The condensed phenols and polyphenols found in the extracts and digests not only showed antioxidant properties against ABTS, FRAP and DPPH radicals, but also had remarkable bioavailability and anticoagulant potential. These results highlight the potential health benefits of phytochemicals from coffee husks and open new perspectives for the use of such compounds in dietary supplements. © 2023, The Authors. All rights reserved.

关键词： Antioxidants

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Effect of Metal Ions, Chemical Agents, and Organic Solvent on Bacillus Sp .R2 Chitinase Activity

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Procedia technology 2016年 22卷 465-470页

作者： Ben Amar Cheba Taha Ibrahim Zaghloul Mohamad Hisham EL-Massry Ahmad Rafik EL-Mahdy Department of Biotechnology Faculty of Nature and Life Sciences University of Sciences and Technology of Oran -Mohamed Boudiaf (USTOMB) BP 1505 Al Mnaouar Oran 31000 Algeria Department of Biotechnology Institute of Post Graduate Studies and Research University of Alexandria Alexandria Egypt Department of Food Science and Technology-Faculty of Agriculture-University of Alexandria- Alexandria-Egypt

Bacillus sp . R 2 purified chitinase was subjected to the effect of metal ions, chemical agents, and organic solvent on its activity, the results showed that K + , Mn 2+ , Na + , Mg 2+ and Ca 2+ stimulated chitinase activity by 2,4,7,15 and 21% respectively whereas Cu 2+ , Fe 2+ , Zn 2+ , Ag + and Hg 2+ inhibited the enzyme. 10 mM β-mercaptoethanol and EDTA decreased the activity by 38.7 and 78.4% respectively whereas 1% SDS inactivated the enzyme completely, also 1 mM galactose, glucose and N-acetyl glucosamine inactivated the enzyme by about 14.4, 25.2 and 38.7% respectively ethanol, acetone and isopropanol inhibited about 30% of chitinase activity however full or nearly full inhibition was observed with chloroform diethylether and ethylacetate. Furthermore the chitinase shelf life was 30 day at -20C and survived 4 freezing and thawing with lost of about 30% of its original activity. This is the first contribution about marine chitinase characterization study from novel gram variable Bacillus isolated from the Red sea.

关键词： Bacillus sp. R2 chitinase characterization metal ions chemical agents organic solvent activity

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Effect of pH and Temperature on Bacillus sp. R2 Chitinase Activity and Stability

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Procedia technology 2016年 22卷 471-477页

作者： Ben Amar Cheba Taha Ibrahim Zaghloul Ahmad Rafik EL-Mahdy Mohamad Hisham EL-Massry Department of Biotechnology Faculty of Nature and Life Sciences University of Sciences and Technology of Oran -Mohamed Boudiaf (USTOMB) BP 1505 Al Mnaouar Oran 31000 Algeria Department of Biotechnology Institute of Post Graduate Studies and Research University of Alexandria Alexandria Egypt Department of Food Science and Technology-Faculty of Agriculture-University of Alexandria- Alexandria-Egypt

The purified chitinase of Bacillus sp. R2 showed a molecular weight of 41.68 KDa, an optimal pH and temperature of 7.5 and 40 °C respectively. The enzyme was stable between pH 7 to 8 but it lost 57% and 62% of its initial activity at the pH 6 and 9 respectively. The purified chitinase also exhibited complete heat stability for 1 h at 40 °C and retained above 50% and 30% of its original activity after 30 min heating at 50 °C and 60 °C respectively. The chitinase activation energy for chitin hydrolysis was 12.93 Kcal/mol. To the best of our knowledge, this is the first study about marine chitinase characterization from novel gram variable Bacillus isolated from the Red sea.

关键词： Bacillus sp. R2 chitinase characterization pH temperature activity stability

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Development of Simultaneous Quantitative Analytical Method for Three Active Components of Agastache Rugosa in Human Plasma Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

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SSRN 2023年

作者： Rinik, Urmi Rahman Kim, Ji Eon Lee, Eunok Kwon, Oran Jung, Byung Hwa Center for Advanced Biomolecular Recognition Korea Institute of Science and Technology Seoul02792 Korea Republic of Division of Bio-medical Science and Technology KIST School University of Science and Technology Daejeon34113 Korea Republic of Department of Nutritional Science and Food Management Graduate Program in System Health Science and Engineering Ewha Womans University Korea Republic of

Abstract: Agastache rugosa contains phenolic compounds and flavonoids, and has been extensively used as a traditional herbal medicine. The major components in Agastache rugosa are rosmarinic acid, tilianin, and acacetin, for which several analytical techniques have been reported. However, these substances have yet to be simultaneously quantified in human plasma. In this study, we aimed to simultaneously determine the three active components of Agastacherugosa in human plasma by developing a reliable quantitative analytical method using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Chromatographic separation of the plasma samples was achieved using an ACQUITY UPLC® BEH C18 column with a gradient mobile phase of water and acetonitrile containing 0.1% formic acid. Mass spectrometric detection was performed using a triple quadrupole tandem mass spectrometer in negative electrospray ionization (ESI−) and multiple reaction monitoring (MRM) modes. The developed quantitative method was validated for the three active components. All three analytes exhibited a linear response over the ranges of 0.5–50 ng/mL for rosmarinic acid, 0.1–20 ng/mL for acacetin, and 0.5–20 ng/mL for tilianin with a weighting factor of 1/x (where x is the concentration). At three quality control (QC) concentration levels (low, medium, and high), including the lower limit of quantitation (LLOQ), acceptable accuracy (±15%) was achieved in the intra- and interday validations. The concentration of rosmarinic acid was highest in plasma. Tilianin and acacetin appeared and were eliminated earlier in the plasma than rosmarinic acid. This study provides a successfully validated method that can be used in further clinical applications of Agastache rugosa extracts. © 2023, The Authors. All rights reserved.

关键词： Mass spectrometers

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Affinity Purification and Immobilization of Chitinase from Bacillus sp .R2

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Procedia technology 2015年 19卷 958-964页

作者： Ben Amar Cheba Taha Ibrahim Zaghloul Ahmad Rafik EL-Mahdy Mohammad Hisham EL-Massry Department of Biotechnology -Faculty of Nature and Life Sciences-University of Sciences and Technology-Oran Mohamed Boudiaf (USTOMB) BP 1505 Al Mnaouar Oran 31000 Algeria Department of Biotechnology Institute of Post Graduate Studies and Research University of Alexandria- Alexandria –Egypt Department of Food Science and Technology-Faculty of Agriculture-University of Alexandria- Alexandria -Egypt

Bacillus sp. R2 chitinase was purified to homogeneity using ammonium sulphate precipitation at 80% saturation, affinity chromatography on swollen crab shell chitin column, and followed by gel filtration chromatography on Sephadex G-100; the enzyme was purified to 14.89-fold and yield of 14.77%, respectively. Furthermore the purified chitinase was subjected to three immobilization techniques on ten various solid carriers;1-physical adsorption immobilization on activated charcoal and silica gel carriers; 2-covalent binding on crab shell chitin and chitosan; 3-Ionic binding on CM-Sepharose, Q-Sepharose, DEAE Cellulose, Amberlite IRC 50, Amberlite CG-120 (NA) and Amberlite CG -4B (OH)respectively. Immobilization results revealed that covalent and ionic binding were the best techniques whereas chitosan, Amberlite IRC50 and chitin gave the highest immobilization yields 72.9, 70.26 and 63.53% with the highest activity yields 63.36, 58.26 and 53.03% respectively. These findings improve the effectiveness of swollen crab shell chitin as matrix for chitinase affinity purification whereas chitin and chitosan as active natural biopolymers for chitinase continuous production through immobilization.

关键词： Bacillus sp .R2 chitin chitinase affinity purification immobilization activity yield

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Effect of bovine colostrum on the growth inhibition and differentiation of human leukemic U937 cells

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Journal of the science of food and Agriculture 2009年第6期89卷 965-969页

作者： Ma, Chia-Yu Kung, Hua-Ting Chen, Jian-Nan Chang, Chi-Yue Chang, Huang-Min Wu, James Swi-Bea Department of Restaurant Management Technology and Science Institute of Northern Taiwan Taipei City 112 Taiwan Graduate Institute of Food Science and Technology National Taiwan University Taipei City 106 Taiwan Department and Graduate Program of Bio Industry Technology Dayeh University Changhua 51591 Taiwan

BACKGROUND: Colostrum is the breast milk of female mammals produced within in a short time after giving birth. It is thought to protect neonates from infection as well as to facilitate immune system maturation. In this study the effect of bovine colostrum on the proliferation and differentiation of human leukemic U937 cells was investigated to understand more about its immunomodulatory activity. RESULTS: Human mononuclear cells (MNCs) were stimulated with bovine colostrum (CS) and then filtered to obtain a conditioned medium (CM) (CS-MNC-CM). CS-MNC-CMs prepared with day 1 to day 4 colostrums inhibited U937 cells by 39.4-64.4%. The expressions of surface markers CD11b and CD14 on U937 cells in the treated groups were 30.6-33.5% and 40.0-42.6% respectively. High levels of cytokines IL-1β, IFN-γ and TNF-α were detected in CS-MNC-CMs. CONCLUSION: Evidence indicates that colostrums stimulate human MNCs to secrete cytokines IL-1β, IFN-γ and TNF-α which subsequently inhibit the growth of U937 cells and induce their differentiation into mature monocytes and macrophages. There is a possibility for bovine colostrum to be processed into an anti-leukemia ingredient for use in health foods. © 2009 Society of Chemical Industry.

关键词： Antitumour Colostrum Cytokine Differentiation Leukemic cell Mononuclear cell

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Chemical composition, bioactivities and kinematic viscosity of cucurbita argyrosperma seed oil

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Chemical Engineering Transactions 2018年 64卷 217-222页

作者： Ribeiro, Pedro R. E. Souza, Vanessa S. Reis de Melo, Ana Cristina G. Neto, Agenor T. M. Santos, Ricardo C. Takahashi, Jacqueline A. Ferraz, Vany P. de Melo Filho, Antonio A. Federal University of Roraima Boa Vista-RR Brazil Departament of Chemistry Center for Research and Graduate Studies in Science and Technology NPPGCT UFRR Campus Paricarana Campus Boa Vista-RRCEP 69304-000 Brazil National Postdoctoral Program of CAPES Universidade Federal de Roraima POSAGRO/UFRR Boa Vista-RR Brazil Department of Chemistry Institute of Exact Sciences Universidade Federal de Minas Gerais Belo Horizonte-MG Brazil Chromatography Laboratory Inst. of Exact Sciences Universidade Federal de Minas Gerais Belo Horizonte-MG Brazil Post-Graduate in Natural Resources Program Federal University of Roraima Boa Vista-RR Brazil Post-Graduate Program in Chemistry PPGQ NPPGCT Departament of Chemistry and Post-Graduate in Biotechnology and Biodiversity Program RedeBionorte Federal University of Roraima Boa Vista-RR Brazil

ISBN: (纸本)9788895608563

The aims of this study were to perform a detailed analysis of the chemical composition by gas chromatography, cinematic viscosity in function of temperature by viscometer Schott AVS 350, and biological tests of abóbora (Cucurbita argyrosperma) seed oil, collected in Mucajai city, Roraima state, Brazil. Thus, the abóbora seed samples were manually submitted to the oven at 50 °C for 72 hours, and crushed and sieved between 20-40 Mesh. The seed oil extraction was carried out in a Soxhlet apparatus with hexane for 3 hours, providing an oil yield of 29.8%. In the results were identified a total of 8 fatty acids by gas chromatography;of these 33.8% are saturated fatty acids and 65.7% are unsaturated fatty acids: palmitic (21.5%), stearic (11.6%), oleic (37.0%), linoleic (28.1%) and linolenic (0.3%). In the biological test, the oil was more active against Staphylococcus aureus (29.01%), Listeria monocytogenes (34.56%), Pseudomonas aeruginosa (23.86%) and Citrobacter freundii (32.74%), which features a characteristic low therapeutic index for these bacteria. The kinematic viscosity of the oil reduces in function of temperature (298.15 to 353.15 K) in an exponential curve, demonstrating to refer to a Newtonian fluid. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Gas chromatography

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Stochastic multi-objective optimization of hybrid distributed power generation with battery storage systems

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Energy Sources, Part B: Economics, Planning, and Policy 2025年第1期20卷

作者： Aglaucibelly Maciel Barbosa Paulo Rotella Junior Rogério Santana Peruchi Luiz Célio Souza Rocha Arthur Leandro Guerra Pires Karel Janda a Post-Graduate Program in Production Engineering and Systems Federal University of Paraiba João Pessoa Brazil b Department of Production Engineering Federal University of Paraiba João Pessoa Brazilc Faculty of Finance and Accounting Prague University of Economics and Business Prague Czech Republicd Institute of Economic Studies Faculty of Social Sciences Charles University Prague Czech Republic b Department of Production Engineering Federal University of Paraiba João Pessoa Brazil e Management Department - Federal Institute of Education Science and Technology - North of Minas Gerais Almenara Brazil f Institute of Production Engineering and Management Federal University of Itajubá Brazil c Faculty of Finance and Accounting Prague University of Economics and Business Prague Czech Republic

ABSTRACTHybrid energy projects are gaining global interest due to their potential to promote sustainable development. This study addresses a gap in the literature by proposing a stochastic model that assesses the economic feasibility of a hybrid energy system combining wind, photovoltaic generation, and battery storage. Unlike traditional deterministic approaches, the model incorporates uncertainties in key financial and operational variables, improving decision-making in real-world conditions. The optimization framework integrates Design of Experiments (DoE), Response Surface Methodology (RSM), and the Desirability function to balance economic return and financial risk. By maximizing the mean and minimizing the variance of the Net Present Value (NPV), the model identifies an optimal system configuration with 92% wind energy, a demand level of 230 kWh/month, and lithium-ion battery storage. This approach reduces computational complexity and enhances hybrid system viability in regions with strong wind resources, providing a valuable tool for investors and policymakers in sustainable energy planning.

关键词： Multiobjective optimization response surface methodology hybrid power system energy storage economic feasibility

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Code Compression in ARM Embedded Systems Using Multiple Dictionaries

Code Compression in ARM Embedded Systems Using Multiple Dict...

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IEEE International Conference on Computational science and Engineering, CSE

作者： Wanderson Roger Azevedo Dias Edward David Moreno Institute of Computing-IComp Post-graduate Program in Informatics-PPGI Federal University of Amazonas Manaus Amazonas Brazil Center for Exact Sciences and Technology-CCET Department of Computer Science-DComp Federal University of Sergipe Aracaju Sergipe Brazil

This paper presents a new code compression method where we use different dictionaries, all of them are based in traditional Huffman algorithm. Our method reduces code size by up to 34.5% (including all extra costs) and presents an improvement of almost 7% over the traditional Huffman method. We performed simulations using Simple Scalar and analyzes using the applications from benchmark MiBench and use the embedded processor ARM.

关键词： Dictionaries Embedded systems Analytical models Benchmark testing Memory management Decoding Energy consumption

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Correction to: Holothuria triterpene glycosides: a comprehensive guide for their structure elucidation and critical appraisal of reported compounds

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Phytochemistry Reviews 2022年第4期21卷 1359-1378页

作者： Puspitasari, Yunita Eka De Bruyne, Tess Foubert, Kenn Aulanni’am, Aulanni’am Pieters, Luc Hermans, Nina Tuenter, Emmy Natural Products and Food Research and Analysis (NatuRA) Department of Pharmaceutical Sciences University of Antwerp Antwerp Belgium Doctoral Program of Environmental Studies Post Graduate University of Brawijaya Malang Indonesia Faculty of Fisheries and Marine Sciences University of Brawijaya Malang Indonesia Faculty of Mathematics and Natural Science Department of Chemistry University of Brawijaya Malang Indonesia

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