检索结果-内蒙古大学图书馆

Improving iPSC Differentiation Using a Nanodot Platform

ACS Applied Materials and Interfaces 2024年第28期16卷 36030-36046页

作者： Chiew, Men Yee Wang, Erick Lan, Kuan-Chun Lin, Yan-Ren Hsueh, Yu-Huan Tu, Yuan-Kun Liu, Chu-Feng Chen, Po-Chun Lu, Huai-En Chen, Wen Liang Center for Regenerative Medicine and Cellular Therapy National Yang Ming Chiao Tung University Hsinchu300 Taiwan Department of Biological Science and Technology National Yang Ming Chiao Tung University Hsinchu300 Taiwan College of Biological Science and Technology Industrial Ph. D. Program National Yang Ming Chiao Tung University Hsinchu300 Taiwan Kyoto University Kyoto606-8397 Japan Department of Emergency and Critical Care Medicine Changhua Christian Hospital Changhua500 Taiwan Department of Post Baccalaureate Medicine College of Medicine National Chung Hsing University Taichung402 Taiwan School of Medicine Kaohsiung Medical University Kaohsiung807 Taiwan School of Medicine Chung Shan Medical University Taichung402 Taiwan College of Biological Science and Technology National Yang Ming Chiao Tung University Hsinchu300 Taiwan Department of Orthopedic Surgery E-Da Hospital I-Shou University Kaohsiung824 Taiwan Emergency Medicine Department Kaohsiung Chang Gung Memorial Hospital Kaohsiung833 Taiwan Ph. D. Degree Program of Biomedical Science and Engineering National Yang Ming Chiao Tung University Hsinchu300 Taiwan Institute of Materials Science and Engineering National Taipei University of Technology Taipei106 Taiwan Institute of Biochemistry and Molecular Biology National Yang Ming Chiao Tung University Hsinchu300 Taiwan Bioresource Collection and Research Center Food Industry Research and Development Institute Hsinchu City300 Taiwan

Differentiation of induced pluripotent stem cells (iPSCs) is an extremely complex process that has proven difficult to study. In this research, we utilized nanotopography to elucidate details regarding iPSC differentiation by developing a nanodot platform consisting of nanodot arrays of increasing diameter. Subjecting iPSCs cultured on the nanodot platform to a cardiomyocyte (CM) differentiation protocol revealed several significant gene expression profiles that were associated with poor differentiation. The observed expression trends were used to select existing small-molecule drugs capable of modulating differentiation efficiency. BRD K98 was repurposed to inhibit CM differentiation, while iPSCs treated with NSC-663284, carmofur, and KPT-330 all exhibited significant increases in not only CM marker expression but also spontaneous beating, suggesting improved CM differentiation. In addition, quantitative polymerase chain reaction was performed to determine the gene regulation responsible for modulating differentiation efficiency. Multiple genes involved in extracellular matrix remodeling were correlated with a CM differentiation efficiency, while genes involved in the cell cycle exhibited contrasting expression trends that warrant further studies. The results suggest that expression profiles determined via short time-series expression miner analysis of nanodot-cultured iPSC differentiation can not only reveal drugs capable of enhancing differentiation efficiency but also highlight crucial sets of genes related to processes such as extracellular matrix remodeling and the cell cycle that can be targeted for further investigation. Our findings confirm that the nanodot platform can be used to reveal complex mechanisms behind iPSC differentiation and could be an indispensable tool for optimizing iPSC technology for clinical applications. © 2024 The Authors. Published by American Chemical Society.

关键词： Efficiency

来源：评论

学校读者我要写书评

暂无评论

Evaluation of Oxalic Acid Extraction and Quantification Methods in the Different Purslane (Portulaca Oleracea L.) Matrices and Spinach (Spinacea Oleracea)

SSRN

引用

SSRN 2024年

作者： de Souza, Pâmela Gomes Pallone, Juliana Azevedo Lima Orlando, Eduardo Adilson Costa-Santos, Augusto César Ayres, Ellen Mayra Menezes Rosenthal, Amauri Teodoro, Anderson Junger Laboratory of Food Analysis Graduate Program in Food Nutrition Fluminense Federal University Rua Mário Santana Braga 30 RJ Niteroi Brazil Laboratory of Food Analysis by Spectroscopy Faculty of Food Engineering State University of Campinas Rua Monteiro Lobato 80 Barão Geraldo SP Campinas Brazil Sensory and Consumer Science Laboratory Postgraduate Program in Food and Nutrition Federal University of the State of Rio de Janeiro Laboratorio Av. Pasteur -Urca 29 Rio de Janeiro Brazil Embrapa Food Technology Av. das América Guaratiba RJ Rio de Janeiro29501 Brazil

Purslane (Portulaca oleracea L.) is considered a Non-Conventional food Plant (NCFP), as it is a vegetable that is underutilized by the population. This specie, like spinach (Spinacea oleracea), has high levels of oxalic acid, an antinutrient that interferes with the bioaccessibility of minerals such as calcium and iron. The valuation of NCFP through scientific research has gained strength over the last few years and the oxalic acid content in vegetables and juices containing conventional or non-conventional vegetables, which have considerable levels of antinutrients, needs to be investigated. Therefore, evaluating and developing new alternative methods that determine the oxalic acid content with less matrix interference, such as the use of Flame Atomic Absorption Spectrometry (FAAS), can contribute to science and consumers. © 2024, The Authors. All rights reserved.

关键词： Sustainable chemistry

来源：评论

学校读者我要写书评

暂无评论

Different Particle Sizes of Momordica Charantia Leaf Powder Modify the Rheological and Textural Properties of Corn Starch-Based 3d food Printing Ink

SSRN

引用

SSRN 2023年

作者： Fan, Meiqi Choi, Young-Jin Wedamulla, Nishala Erandi Oh, Mirae Bae, Sung Mun Yang, Daeun Kang, Hyo Tang, Yujiao Moon, Sang-Ho Kim, Eun-Kyung Division of Food Bioscience College of Biomedical and Health Sciences Konkuk University Chungju27478 Korea Republic of Department of Food Science and Nutrition College of Health Science Dong-A University Busan49315 Korea Republic of Department of Health Sciences The Graduate School of Dong-A University Busan49315 Korea Republic of Department of Food Science and Technology Faculty of Animal Science and Export Agriculture Uva Wellassa University Badulla90000 Sri Lanka Grassland and Forages Division National Institute of Animal Science Rural Development Administration Cheonan31000 Korea Republic of Gyeongnam Agricultural Research and Extension Services Jinju52733 Korea Republic of BK-21 Four Graduate Program Department of Chemical Engineering Dong-A University Busan49315 Korea Republic of School of Bio-Science and Food Engineering Changchun University of Science and Technology Changchun130600 China Center for Food and Bio Innovation Dong-A University Busan49315 Korea Republic of Nutritional Education Major Graduate School of Education Dong-A University Busan49315 Korea Republic of Nutrinomics Lab. Co. Ltd. Busan49315 Korea Republic of

The study determined the effect of incorporating Momordica charantia leaf powder (MCLP) into corn-starch 3D food-printing ink as a functional ingredient. The effects of the particle size (75, 131, and 200 μm) and quantity of MCLP on 3D printing performance, structural, textural, and rheological properties of corn starch gel were evaluated with different concentrations (5, 10, and 15% (w/w)) of corn starch. The viscoelastic properties of food inks were determined considering their behavior during extrusion and self-recovery after printing. Scanning electron microscope was used to characterize the microstructure. Based on the results, a high starch content (15%) with 5% MCLP was more favorable for 3D food printing. In addition, 3D printing performance, textural and rheological properties of formulated ink was mainly governed by the particle size of MCLP. The food ink with a 5% mass fraction of 200 μm MCLP had the highest printing precision and the best masticatory properties. © 2023, The Authors. All rights reserved.

关键词： Particle size

来源：评论

学校读者我要写书评

暂无评论

Fish and seaweed (Eucheuma cotonii) meatball for reducing constipation effect

引用

AIP Conference Proceedings 2019年第1期2155卷

作者： I. Made Sugitha I. Putu Suparthana Pinky Natalia Samanta 1Food Science and Technology Study Program Faculty of Agricultural Technology Udayana University Bukit Jimbaran Badung Bali Indonesia 2Post-graduate Program Udayana University Bukit Jimbaran Badung Bali Indonesia

Functions of dietary fiber include increasing stool mass and reducing intestinal transit time which can be used to prevent constipation. This study aims to determine the ability of mixed catfish meatballs and sea grass flour to reduce constipation through two stages. The first stage was to get the best meatball quality (ratio of tapioca composition and seaweed flour manipulated at 100%: 0%; 90%: 10%; 80%: 20%; 70%: 30%) followed by the second stage which was in vivo testing for rat with doses of meatballs manipulated at 2 g, 4 g, 6 g and 8 g. Research data were analyzed with one-way variance analysis with a confidence level of 95%. Composition of tapioca and seaweed flour at 70%: 30% was the best fish meatball formula resulting in these data: 64.90% moisture content, 2.00% ash, 5.76% fat, 13.91% protein, 13.43% carbohydrate, total food fiber 5.94%) with sensory value: appearance 8.67 (smooth, not hollow, and bright surface), flavor 7.00 (less fish flavor), taste 6.67 (less meat fish taste), texture 9 (solid, compact, springy). The in vivo test showed that administration of 2 g / 200 g weigh body of rat was able to produce rat feces back to normal condition (47.93% moisture content) with a frequency of defecation 21 times per day (no constipation any more).

关键词：

来源：评论

学校读者我要写书评

暂无评论

Revisiting some overlooked registered Thai traditional medicine preparations: an alert to a regulatory affair

引用

Bulletin of the National Research Centre 2022年第1期46卷 1-9页

作者： Vimolmangkang, Sornkanok Rani, Dolly Prestwong, Naruemon Sutcharitchan, Chayanis Pakdeesattayapong, Duangkamol Department of Pharmacognosy and Pharmaceutical Botany Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok Thailand Center of Excellence in Plant Produced Pharmaceuticals Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok Thailand Graduate Program in Pharmaceutical Science and Technology Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok Thailand Herbal Products Division Food and Drug Administration Thailand Ministry of Public Health Nonthaburi Thailand

This study evaluated the heavy metal identity and content of registered Thai Traditional medicine (TTM) preparations in Thailand to ensure consumer safety. Both qualitative and quantitative information about the elemental composition of the heavy metal content in each TTM crude drug and the heavy metal content in some registered TTM preparations in Thailand was performed. Seven kinds of mineral-based TTM crude drugs, namely realgar, orpiment, Chinese powder, Sen, vermilion, natural vermilion, and cinnabar, were analyzed with X-ray diffraction and scanning electron microscopy and energy-dispersive spectroscopy techniques to determine the type of metal and elemental composition to confirm their chemical formulas. Furthermore, the number of heavy metals, such as arsenic (As), lead (Pb), and mercury (Hg), was in three samples of realgar-containing preparations; one sample of Sen-containing preparation, and eight samples of cinnabar-containing preparations. Heavy metals were determined with inductively coupled plasma-optical emission spectrometry. It was observed that realgar and orpiment consisted of arsenic sulfide (AsS) and arsenic trisulfide (As2S3), respectively. Both crude drugs contained approximately 50% of As. Vermilion, natural vermilion, and cinnabar consisted of mercuric sulfide (HgS), with Hg accounting for approximately 66%. Sen consisted of Pb (II, IV) oxide (Pb3O4) with an approximate Pb percentage of 80%. However, Pb was absent in Chinese powder and consisted mainly of calcium carbonate (CaCO3). A traditional detoxification procedure can reduce the amount of As and Hg in processed crude drugs by at least 20%. Pb was higher in some TTM preparations, and no CaCO3 was detected in Chinese powder. Our results raise concerns on both safety and efficacy to consumers and alert public health policymakers that they should implement regulations so that the quality (authenticity) and quantity of elemental medicine used in traditional medicine are correctly labeled a

关键词：

来源：评论

学校读者我要写书评

暂无评论

Preface: Marine and coastal applications in landscape ecology

引用

Landscape Ecology 2008年第SUPPL. 1期23卷 1-5页

作者： Hinchey, Elizabeth K. Nicholson, Matthew C. Zajac, Roman N. Irlandi, Elizabeth A. U.S. Environmental Protection Agency National Health and Environmental Effects Research Laboratory Atlantic Ecology Division Narragansett RI 02882 27 Tarzwell Dr United States Illinois-Indiana Sea Grant College Program Purdue University Chicago IL 60604 77 West Jackson Blvd. (G-17J) United States U.S. Environmental Protection Agency Environmental Information and Analysis Branch Environmental Assessment and Innovation Division Philadelphia PA 19103-2029 1650 Arch Street United States Graduate Program in Environmental Science Department of Biology and Environmental Science University of New Haven West Haven CT 06516 300 Boston Post Road United States Department of Marine and Environmental Systems Florida Institute of Technology Melbourne FL 32901 150 West University Boulevard United States

Landscape ecology traditionally has been limited to the study of terrestrial systems;however, the questions and methods defining the science are equally relevant for marine and coastal systems. The reciprocal relationship between spatial pattern and ecological processes and the overarching effect of scale on this relationship was being explored in some marine and coastal settings as the general discipline of landscape ecology was evolving throughout the latter two decades of the last century. As with all components of the biosphere, an understanding of these relationships is critical for successful management of marine and coastal systems. In these systems, widely dispersed field or ship-based observations and lack of broad scale data have historically precluded quantification of large-scale patterns and processes and hindered management efforts. However, relatively recent advances in geographic information systems, remote sensing and computer technologies have begun to address these issues and are now permitting assessments of pattern and process in oceans. The intent of this special issue is to highlight research that is adapting the tools of landscape ecology to answer ecological questions within marine and coastal systems, to address the unique challenges faced in these landscapes, and to stimulate an exchange of ideas and solutions to common problems. Inspiration for this special issue of Landscape Ecology began with a special session on "Marine and Coastal Applications in Landscape Ecology" that was held at the 19th Annual Symposium of the United States Regional Association of the International Association for Landscape Ecology, March 31-April 2, 2004 in Las Vegas, Nevada. © 2007 Springer science+Business Media B.V.

关键词： Benthic landscapes Habitat fragmentation Landscape ecology Larval dispersal Marine coral reef reserves Polychaete species diversity Reef fish communities Seagrass landscape pattern Sediment metal concentrations

来源：评论

学校读者我要写书评

暂无评论

Patient-generated subjective global assessment and computed tomography in the assessment of malnutrition and sarcopenia in cirrhotic patients

引用

Clinical Nutrition 2018年 37卷 S65-S66页

作者： M.C. Gonzalez D.N. Zambrano J. Xiao C.M. Prado Post-graduate Program in Health in Behavior Catholic University of Pelotas Pelotas Brazil Department of Agricultural Food and Nutritional Science University of Alberta Edmonton Canada

来源：评论

学校读者我要写书评

暂无评论

Role of plant genetic resources in encountering climate change challenge 10

Role of plant genetic resources in encountering climate chan...

引用

10th Annual International Conference 2020 on Environmental and Life sciences, AIC-ELS 2020

作者： Ichsan, C.N. Darusman Sulaiman, M.I. Andini, R. Doctoral Program in Agricultural Sciences Post Graduate School Universitas Syiah Kuala Jl. Tgk. Chik Pante Kulu No.3 Banda Aceh Indonesia Department of Soil Science Universitas Syiah Kuala Jl. Tgk. Hasan Krueng Kalee No. 3 Banda Aceh Indonesia Department of Agricultural Product Technology Universitas Syiah Kuala Jl. Tgk. Hasan Krueng Kalee No. 3 Banda Aceh Indonesia Department of Forestry Universitas Syiah Kuala Jl. Tgk. Hasan Krueng Kalee No. 3 Banda Aceh Indonesia

The world is encountering a global threat: Climate change. It is estimated that the average air temperature will raise 3.7-7.8 °C by 2100. The possible impacts will vary, however, agriculture will be adversely affected marked with yield loss of staple crops such as rice and maize and altered post-harvest quality of horticulture products. On the other hand, the world food and Agriculture Organization (UN-FAO) has set a target 'zero hunger' by 2030', despite the five big challenges ahead: I) protracted conflict, ii) growing inequality, iii) rapid urbanization, iv) economic challenges, v) drought and natural disasters. The existing plant genetic resources, particularly the wild, neglected, and underutilized species has a great role and bears big potential to be exploited and targetted to achieve food and nutrition security, esp. in the densely populated regions with malnutrition problem e.g. in Asia and sub-Saharan regions. However, a key to future and sustainable crop production lies in the collection of crop and their wild relatives stored in ex-situ, or in-situ. This paper aims to explain the importance of plant genetic resources as well as the potential of some underutilized crops closely attributed with drought resistence, such as sorghum (Sorghum bicolor L. Moench), amaranth (Amaranthus sp.). These crops bear potential to support national food security program. © Published under licence by IOP Publishing Ltd.

关键词： Crops

来源：评论

学校读者我要写书评

暂无评论

Neutrophil-targeted combinatorial nanosystems for suppressing bacteremia-associated hyperinflammation and MRSA infection to improve survival rates

引用

Acta Biomaterialia 2024年 174卷 331-344页

作者： Chang, Yen-Tzu Lin, Cheng-Yu Chen, Chih-Jung Hwang, Erica Alshetaili, Abdullah Yu, Huang-Ping Fang, Jia-You Pharmaceutics Laboratory Graduate Institute of Natural Products Chang Gung University Taoyuan Kweishan Taiwan Department of Pathology and Laboratory Medicine Taichung Veterans General Hospital Taichung Taiwan School of Medicine Chung Shan Medical University Taichung Taiwan Department of Post-Baccalaureate Medicine College of Medicine National Chung Hsing University Taichung Taiwan Department of Dermatology Yale School of Medicine Yale University New HavenCT United States Department of Pharmaceutics College of Pharmacy Prince Sattam Bin Abdulaziz University Al Kharj Saudi Arabia Department of Anesthesiology Chang Gung Memorial Hospital Taoyuan Kweishan Taiwan School of Medicine College of Medicine Chang Gung University Taoyuan Kweishan Taiwan Research Center for Food and Cosmetic Safety and Research Center for Chinese Herbal Medicine Chang Gung University of Science and Technology Taoyuan Kweishan Taiwan

There is currently no specific and effective treatment for bacteremia-mediated sepsis. Hence, this study engineered a combinatorial nanosystem containing neutrophil-targeted roflumilast-loaded nanocarriers and non-targeted fusidic acid-loaded nanoparticles to enable the dual mitigation of bacteremia-associated inflammation and methicillin-resistant Staphylococcus aureus (MRSA) infection. The targeted nanoparticles were developed by conjugating anti-lymphocyte antigen 6 complex locus G6D (Ly6G) antibody fragment on the nanoparticulate surface. The particle size and zeta potential of the as-prepared nanosystem were about 200 nm and −25 mV, respectively. The antibody-conjugated nanoparticles showed a three-fold increase in neutrophil internalization compared to the unfunctionalized nanoparticles. As a selective phosphodiesterase (PDE) 4 inhibitor, the roflumilast in the nanocarriers largely inhibited cytokine/chemokine release from the activated neutrophils. The fusidic acid-loaded nanocarriers were vital to eliminate biofilm MRSA colony by 3 log units. The nanoparticles drastically decreased the intracellular bacterial count compared to the free antibiotic. The in vivo mouse bioimaging demonstrated prolonged retention of the nanosystem in the circulation with limited organ distribution and liver metabolism. In the mouse bacteremia model, the multifunctional nanosystem produced a 1‒2 log reduction of MRSA burden in peripheral organs and blood. The functionalized nanosystem arrested the cytokine/chemokine overexpression greater than the unfunctionalized nanocarriers and free drugs. The combinatory nanosystem also extended the median survival time from 50 to 103 h. No toxicity from the nanoformulation was found based on histology and serum biochemistry. Furthermore, our data proved that the active neutrophil targeting by the versatile nanosystem efficiently alleviated MRSA infection and organ dysfunction caused by bacteremia. Statement of significance: Bacteremia-mediate

关键词： Nanoparticles

来源：评论

学校读者我要写书评

暂无评论

The Standardized Wood Extract of Albizia myriophylla: Its Potential as an Active Ingredient in an Anti-inflammatory Herbal Gel Formulation

引用

Planta Medica 2021年第12期88卷 964-974页

作者： Nazneen Bakasatae Warissara Sangkaew Nongluk Kunworarath Julalak Chorachoo Ontong Sathianpong Phoopha Chutha Takahashi Yupanqui Supayang Piyawan Voravuthikunchai Nantiya Joycharat Traditional Thai Medical Research and Innovation Center Faculty of Traditional Thai Medicine Prince of Songkla University Songkhla Thailand Cosmetic Technology and Dietary Supplement Products Program Faculty of Agro and Bio Industry Thaksin University Phatthalung Thailand Interdisciplinary Graduate School of Nutraceutical and Functional Food Prince of Songkla University Songkhla Thailand Excellent Research Laboratory on Natural Products Faculty of Science and Natural Product Research Center of Excellence Prince of Songkla University Songkhla Thailand Division of Biological Science Faculty of Science Prince of Songkla University Songkhla Thailand

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：