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Microbe (Netherlands) 2025年 6卷

作者： Casagrande, Camilla Voos, Monica Nunes, Franciane Batista dos Santos Alves, Camilla Filippi Verdi, Camila Marina Barin, Juliano Santos, Roberto Christ Vianna Department of Microbiology and Parasitology Federal University of Santa Maria (UFSM) Santa Maria Brazil Department of Food Science and Technology Federal University of Santa Maria Rio Grande do Sul Santa Maria Brazil Laboratory of Oral Microbiology Research (LAPEMICRO) Federal University of Santa Maria Rio Grande do Sul Brazil Graduate Program in Pharmaceutical Sciences Federal University of Santa Maria Rio Grande do Sul Brazil

Quorum sensing (QS) strongly regulates the biofilm formation mechanism in several microorganisms. The blockade of violacein production in Chromobacterium violaceum has been employed to indicate QS inhibition. Nevertheless, quantifying violacein is challenging due to the reliance on high-cost equipment and supplies. Consequently, this study compared the production and inhibition of violacein using a traditional spectrophotometric method and an alternative approach utilizing a Photometrix UVC Android application integrated with a USB endoscopic camera. The study standardized the growth of C. violaceum in several culture media and incubation times, then evaluated the pigment inhibition against salicylic acid. Results indicated that C. violaceum produced more violacein when cultivated in BHI broth for 24 h. Salicylic acid inhibited violacein production, even at sub-inhibitory concentrations, with a minimum inhibitory pigment concentration (125 µg/mL) and a minimum bactericidal concentration (2000 µg/mL). A comparison between application-based readings and the traditional method showed no significant differences, highlighting the potential of this technology as an essential tool in for QS inhibition and biofilm research. © 2025

关键词： Biofilm Colorimetric methods Smartphone Violacein

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Ginger infusion increases diet-induced thermogenesis in healthy individuals:A randomized crossover trial

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food Bioscience 2022年第6期50卷 314-319页

作者： Ana Paula da Costa Soares Natália Cristina de Faria Guilherme Fonseca Graciano James Almada da Silva Vitoria Shievila dos Santos Gonçalves Virginia Del Carmen Trancoso Valenzuela Maria Isabel Toulson Davisson Correia Lucilene Rezende Anastácio Post-Graduate Program in Food Science Pharmacy SchoolUniversidade Federal de Minas GeraisBrazil Surgical Post-Graduate Program Alfa Institute of GastroenterologyHospital das ClínicasUniversidade Federal de Minas GeraisBrazil Pharmacy Department Universidade Federal de SergipeBrazil Fundação Ezequiel Dias Brazil

The purpose of this study is to evaluate the acute effect of ingesting ginger infusion on energy metabolism,appetite responses,and food intake in healthy *** is a clinical(RBR-9ZJGQM),randomized,open,and crossover study with a minimum interval of seven days between *** drinks,ginger infusion(2 g)and water(control)were served with a standard *** has 21 volunteers of both sexes(18–40 years old,18.5–26 kg/m^(2)).Consumption of ginger tea increased on average 95.5±27.4 kcal/day the non-nitrogen energy expenditure after 240 min of ingestion(p=0.002).There was a significant effect of ginger on diet-induced thermogenesis(difference between ginger tea treatment ***:113.0±43.0 kcal/day,p=0.016).However,appetitive responses and prospective food intake did not differ between ginger tea and water intake.

关键词： Zingiber officinale Ginger Indirect calorimetry Energy metabolism

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Transmission of real-time video signal with interference density and human traffic

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Telkomnika (Telecommunication Computing Electronics and Control) 2015年第4期13卷 1352-1360页

作者： Bahaweres, Rizal Broer Karya, Oki Teguh Alaydrus, Mudrik Post-Graduate Program of Electrical Engineering Departement Mercu Buana University Indonesia KompasTV Indonesia Faculty of Science and Technology State Islamic University Syarif Hidayatullah Jakarta Indonesia

The use of mobile phone as a communication tool rapidly increases, as well as various types of functions therein. Among of the many applications on the mobile phone, which are used skype androidbased for teleconferencing via mobile phone. Things can not be separated from these applications is the need of qualified internet network access for these applications can be felt up function. One of the network access to the Internet is widely used today's society is a wi-fi network. Access to the Internet does not always provide the best performance, this is due to many factors, one of which is the presence of interference. In this study, we tested transmission of video signals in real-time using an application skype on the mobile phone. Skype run on wi-fi network, which is influenced by the presence of interference or objects such as human traffic in the network. Wireshark were used to obtain data reliability wi-fi network, known as QoS. While evalvid used to obtain the data QoE restricted only get the data PSNR (Peak Signal To Noise Ratio) and MSE (Mean Square Error). © 2015 Universitas Ahmad Dahlan.

关键词： Wi-Fi

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Mogroside V inhibits LPS-induced COX-2 expression/ROS production and overexpression of HO-1 by blocking phosphorylation of AKT1 in RAW264.7 cells

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生物化学与生物物理学报（英文版） 2019年第4期51卷 365-374页

作者： Yong Li Luyan Zou Tao Li Dengni Lai Yanyang Wu Si Qin Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China Core Research Program 1515 Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and Technology Hunan Agricultural University Changsha 410128 China The United Graduate School of Agricultural Sciences Faculty of Agriculture Kagoshima University Korimoto 1-21-24 Kagoshima 890-0065 Japan

Momordica grosvenori is a valuable edible plant with medicinal purposes,and it is widely used in medicated diets and traditional Chinese medicine in *** V (MV),the main bioactive component from ***,is commonly used as a natural *** extracts have been reported to exert potent anti-inflammatory property,however the underlying molecular mechanism still remains *** the present study,the biological effect of MV in inflammation was investigated in lipopolysaccharide (LPS)-stimulated RAW264.7 *** ELISA and western blot analysis results showed that MV significantly inhibited LPS-induced prostaglandin E2 (PGE2) production and cyclooxygenase-2 (COX-2) *** markedly decreased the phosphorylation of IκB-α,increased IκB-α,and reduced nuclear p-65 and C/EBPδ.Furthermore,MV attenuated LPS-induced phosphorylation of MAPKs and AKT1,and only the phosphorylation status of AKT1 was found to be consistent with the expression trend of ***,MV reduced ROS level and restored overexpressed HO-1 and AP-1 to basal level,which can be markedly reversed by AKT1 inhibitor *** results revealed that AKT1 plays a key role in LPS-induced COX-2 expression,and acts as a mediator to keep the redox balance in LPS-stimulated RAW264.7 *** exerts anti-inflammatory property by blocking AKT1-mediated NF-κB and C/EBPδ activation,ROS generation and AP-1/HO-1 ***,the present study indicated that MV has a significant chemopreventive effect on the inflammatory lesions and suggested that AKT1 is a potential specific target of MV for relieving inflammation.

关键词： Mogroside V anti-inflammatory property HO-1 NF-κB AKT1 ROS

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Functional diversity of AI-2/LuxS system in lactic acid bacteria: Impacts on biofilm formation and environmental resilience

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Research in Microbiology 2025年 104296页

作者： Kanthenga, Hopeful Tusalifye Banicod, Riza Jane S. Ntege, Wilson Njiru, Moses Njeru Javaid, Aqib Tabassum, Nazia Kim, Young-Mog Khan, Fazlurrahman KOICA-PKNU International Graduate Program of Fisheries Science Pukyong National University Busan 48513 South Korea Department of Fisheries Malawi College of Fisheries Mangochi 301401 Malawi Fisheries Postharvest Research and Development Division National Fisheries Research and Development Institute Quezon City 1103 Philippines Fisheries Control Regulation and Quality Assurance Ministry of Agriculture Animal Industry and Fisheries Entebbe 10101 Uganda Department of Fisheries and Aquaculture Turkana County Government Lodwar 30500 Kenya Interdisciplinary Program of Marine and Fisheries Sciences and Convergent Technology Pukyong National University Busan 48513 South Korea Marine Integrated Biomedical Technology Center The National Key Research Institutes in Universities Pukyong National University Busan 48513 South Korea Research Center for Marine Integrated Bionics Technology Pukyong National University Busan 48513 South Korea Department of Food Science and Technology Pukyong National University Busan 48513 South Korea Ocean and Fisheries Development International Cooperation Institute Pukyong National University Busan 48513 South Korea International Graduate Program of Fisheries Science Pukyong National University Busan 48513 South Korea

A key component of microbial communication, autoinducer-2 (AI-2) signaling, affects several physiological processes, including environmental adaptation and biofilm formation in lactic acid bacteria (LAB). The multifarious contribution of AI-2, synthesized by LuxS, in improving biofilms and tolerance to hostile conditions in LAB has been investigated in this review. The evolutionary conservation and diversity of AI-2 are shown by a phylogenetic analysis of luxS gene among several LAB species. Furthermore, AI-2 signaling in LAB improves resistance to unfavorable environmental factors, including pH fluctuations, temperature extremes, and antimicrobial agents. Lactic acid bacteria could set off defenses against harmful impacts from environmental stresses. © 2025 Institut Pasteur

关键词： Autoinducer-2 Biofilm formation Environmental adaptation Lactic acid bacteria LuxS enzyme Phylogenetic analysis

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Enhancing Diagnostic Accuracy for Knee Osteoarthritis Using a Deep Learning Approach

Enhancing Diagnostic Accuracy for Knee Osteoarthritis Using ...

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International Symposium on Applied science 2024, ISAS 2024

作者： Raza, Aquib Ching, Congo Tak Shing Liu, Peng-Ta Phan, Thien-Luan Tran, Ngoc-Luan Nguyen, Ha Anh T. Rameez, Mohammad Li, Hung-Chung Linh, Dao Nguyen Phuong Van Hieu, Nguyen Tran, Trung Nghia National Taiwan University Taipei City10673 Taiwan Research Center for Information Technology Academia Sinica Taipei City11529 Taiwan Graduate Institute of Biomedical Engineering National Chung Hsing University Taichung402 Taiwan Department of Electrical Engineering National Chi Nan University Nantou County Taiwan Advanced Plant and Food Crop Biotechnology Center National Chung Hsing University Taichung402 Taiwan International Doctoral Program in Agriculture National Chung Hsing University Taichung402 Taiwan Fall Prevention Center Department of Physical Medicine & Rehabilitation Changhua Christian Hospital Changhua500 Taiwan Department of Physics and Electronic Engineering University of Science Vietnam National University - Ho Chi Minh City Ho Chi Minh City70000 Viet Nam Department of Computer Science University of York York HeslingtonYO10 5DD United Kingdom Undergraduate Program of Intellectual Creativity Engineering National Chung Hsing University Taichung402 Taiwan Ho Chi Minh City72409 Viet Nam HCMUT Vietnam National University Ho Chi Minh City Thu Duc Ho Chi Minh City71308 Viet Nam

This research introduces a deep learning framework that combines convolutional neural networks with autoencoders to improve the diagnostic accuracy of knee osteoarthritis. The study utilized a publicly available dataset from the Mendeley Data repository, where knee joint images were preprocessed using Contrast Limited Adaptive Histogram Equalization to enhance contrast and ensure consistent image sizing. Initially, an autoencoder was employed in an unsupervised manner to learn compressed representations of the data. These representations were then used to train a CNN to classify knee images into 'healthy' and 'osteoarthritic' categories. The model was trained using an 80:20 split for training and testing data, incorporating early stopping and data augmentation techniques to prevent overfitting and enhance generalization. Performance metrics such as accuracy, precision, recall, and the area under the Receiver Operating Characteristic curve (ROCAUC) were used to evaluate the model, resulting in a high test accuracy of 98.95%. The high precision and recall rates further confirmed the model's robustness in diagnosing KOA. To enhance interpretability, Gradient-weighted Class Activation Mapping (Grad-CAM) was applied to identify critical regions in the images that influenced the model's decisions. Additionally, t-Distributed Stochastic Neighbor Embedding (t-SNE) was used to visualize the high-dimensional encoded features in a two-dimensional space, providing deeper insights into how the model differentiates between classes. The promising results of this deep learning approach suggest its potential for broader applications in medical imaging diagnostics. By providing healthcare professionals with a tool that enhances diagnostic accuracy and operational efficiency, this framework lays the foundation for developing scalable, AI-driven diagnostic systems for various complex medical conditions. © 2025 Institute of Physics Publishing. All rights reserved.

关键词： Joints (anatomy)

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Effects of a Combination of Phytoestrogens on Risk Factors for Cardiovascular Diseases in Climacteric Women: A Randomized, Placebo-controlled Trial

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Current Bioactive Compounds 2023年第2期19卷 20-28页

作者： de Barros, Evayne dos Santos, Paola Cristine de Bortoli Frigo, Maiara Weber, Jucieli Zanelatto, Carla Koehnlein, Eloá Angélica Course of Nutrition Federal University of Fronteira Sul Realeza Brazil Post Graduate Program in Food Science and Technology Federal University of Fronteira Sul Laranjeiras do Sul Brazil University center of Pato Branco Brazil

Background & Aims: The incidence of cardiovascular disease in climacteric women has increased, becoming the main cause of death in mature women. The use of phytoestrogens, isoflavones, and lignans derived from soy and flaxseed can be natural alternative treatments for this phase of life. This study investigates the utility of isoflavones and lignans derived from soy and flaxseed as natural alternatives to pharmaceuticals to alleviate the symptoms of menopause. Methods: A clinical, prospective, randomized, blind, placebo-controlled trial was performed. For-ty-eight women aged 40 to 65 years were divided into two groups, Phytoestrogen (PHY), which received a cereal bar containing isoflavones and lignans for 90 days, and Placebo (PLA), which received expanded rice flake bar. The sociodemographic, clinical, anthropometric data, blood pressure (BP) measurements, and biochemical and hormonal tests were performed. Results: Forty-three women were included in this study (PHY=21 and PLA=22). There was a significant reduction in diastolic BP in the PHY group after the intervention (p<0.001). The frequen-cy of BP fluctuations in the PHY group significantly decreased to 23.8% after the intervention period (p=0.027), with no significant differences for the other indicators studied. Conclusion: The combination of phytoestrogens showed a trend in reducing the blood pressure of climacteric women. However, no significant changes in cardiovascular risk factors were observed. Clinical Trial Registration: The clinical trial was registered on the REBEC-Brazilian Clinical Trials Registry, number 6z8qqy (https://***/). © 2023 Bentham science Publishers.

关键词： cereal bar estrogen deficiency isoflavones lignans lipid metabolic disorder Menopause

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Microencapsulated phenolic-rich extract from juice processing grape pomace(Vitis *** Var):Effects on oxidative stability of raw and pre-cooked bovine burger

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food Bioscience 2022年第6期50卷 2120-2129页

作者： Marcelo Edvan dos Santos Silva Rodrigo Lira de Oliveira Thamyres Cesar de Albuquerque Sousa Cristiani Viegas Brandão Grisi Valquíria Cardoso da Silva Ferreira Tatiana Souza Porto Marta Suely Madruga Suzana Pedroza da Silva Fábio Anderson Pereira da Silva Post-Graduate Program in Food Science and Technology Department of Food EngineeringTechnology CentreFederal University of ParaibaJoão PessoaParaiba58051-900Brazil Northeast Biotechnology Network/RENORBIO Federal Rural University of Pernambuco (UFRPE)Dom Manoel de MedeirosDois Irmãos52171-900RecifePEBrazil Federal University of Pernambuco's Agreste (UFAPE) GaranhunsAv.Bom PastorBoa Vista55296-901 GaranhunsPEBrazil Post-Graduate Program in Agrifood Technology Federal University of ParaibaCampus Universitario IIIBananeirasPBBrazil

Grape processing generates residues rich in bioactive compounds with potential application in the meat ***,these substances are unstable and needs to be treated to allow their action during the processing and storage of meat *** this sense,a phenolic-rich extract from grape pomace was microencapsulated and evaluated for its potential to inhibit the oxidative process in raw and precooked beef burgers during cold *** hamburger formulations containing lyophilized extract(LEB),microencapsulated extract(MEB)and synthetic antioxidant sodium erythorbate(SEB)were prepared,pre-cooked,and evaluated for lipid and protein *** microencapsulation,with 95.0%efficiency,provided a material with a crystalline structure,rich in phenolics and with high antioxidant potential,showing 377.4±0.01,1768.1±0.07 and 1185.0±0.20μmol TEAC.100 g^(−1) for DPPH,ABTS and FRAP,*** values of malonaldehyde were found in LEB and MEB(2.9±0.3 and 1.4±0.5 mg ***^(−1),respectively)at the end of storage,with emphasis on for the hamburgers that received the microencapsulated *** behavior was observed for the analysis of carbonyl compounds,with values of 11.1±1.1,8.7±1.4 and 6.8±1.0 nM ***^(−1) in SEB,LEB and MEB,***,the freeze-dried and microencapsulated grape extracts showed superior results when compared to the synthetic antioxidant,increasing the oxidative stability of hamburgers,and avoiding significant color changes during the cold *** results indicate the high potential of the microencapsulated material to be used as a natural antioxidant in the meat industry.

关键词： Lipid oxidation Protein oxidation Meat products Natural antioxidant Encapsulation

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Analysis of synthetic natural gas injection into charcoal blast furnace

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Journal of Materials Research and technology 2013年第3期2卷 255-262页

作者： Rocha, Elisa Pinto Da Guilherme, Vagner Silva De Castro, José Adilson Sazaki, Yasushi Yagi, Jun-Ichiro Post-Graduation Program in Metallurgical Engineering Universidade Federal Fluminense Volta Redonda RJ Brazil Environmental Metallurgy Laboratory Graduate Institute of Ferrous Technology Pohang University of Science and Technology Pohang Korea Republic of Tohoku University Sendai Japan

Recently, the synthetic natural gas (SNG) has been available for large consumers in the Brazilian domestic market. The SNG is a suitable fuel with potential use to several processes and facilities of the steel industry. This paper investigates the injection of SNG into charcoal blast furnaces as a substitute for granular charcoal charged from the top and pulverized charcoal injections with potential increase of productivity and decrease of specific CO2 emissions. In order to study the effect of replacement of granular charcoal and injection of pulverized charcoal the well known Blast Furnace Five Fluid Model (BF-FFM) is applied to describe detailed phenomena within the process. This model treats the blast furnace as a five-phase reactor, accounting for gas, lump solids (raw iron ore, sinter, pellets and granular charcoal), hot metal, pulverized charcoal, and molten slag. The model is based on conservation equations for mass, momentum, energy and chemical species numerically solved based on the finite volume method (FVM). The SNG has high amount of H2 and strongly affects the inner conditions of the reactor and only a comprehensive model with detailed chemical reaction rates can properly predict the inner parameters of the process and hence furnish useful information to design smooth operational conditions. The simulation results obtained in this study indicated that smooth operations using SNG up to 25 N m3/t of pig iron could be achieved simultaneously by increasing the productivity up to 58% and with a decrease of 40 kg/thm on the granular charcoal charged. © 2013 Brazilian Metallurgical, Materials and Mining Association.

关键词： Steelmaking

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Using puzzle to encourage students to do problem posing

Using puzzle to encourage students to do problem posing

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International Conference on Mathematics and Natural sciences 2017, IConMNS 2017

作者： Candiasa, I.M. Santiyadnya, N. Sunu, G.K.A. Educational Science Study Program Post-Graduate Faculty Universitas Pendidikan Ganesha Bali81116 Indonesia Electronica Technology Education Study Program Faculty of Technology and Vocational School Universitas Pendidikan Ganesha Bali81116 Indonesia Educational Administration Post-Graduate Program Faculty Universitas Pendidikan Ganesha Bali81116 Indonesia

Problem posing is very important in learning mathematics. Many studies have indicated that problem posing can improve the ability to solve mathematics problems. Therefore it is necessary to encourage students systematically to do problem posing. This research tries to pack problem posing in puzzle frame in order to increase student's interest and ability to do problem posing. The puzzle game which is then directed to problem posing is assured can arouse students' enthusiasm, thus encouraging their involvement in problem posing activities. Two groups of junior high school students each consisted of 31 and 32 students selected as samples. The first group follow mathematics class with problem posing learning model that preceded by playing a puzzle but still in a mathematical environment. The second group follow the mathematics class with problem posing learning model only. At the end of the research student's interest and ability to do problem posing are compared by using MANOVA test. Wilks' Lambda test results F = 10.8 on significance (p) = 0.002. Based on these findings, it is concluded that the problem posing learning model in mathematics that is preceded by playing puzzles can increase students' interest and ability to do problem posing. © Published under licence by IOP Publishing Ltd.

关键词： Students

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