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Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes

Environmental science and Ecotechnology 2024年第4期20卷 177-188页

作者： Zhiguang Qiu Yuanyuan Zhu Qing Zhang Xuejiao Qiao Rong Mu Zheng Xu Yan Yan Fan Wang Tong Zhang Wei-Qin Zhuang Ke Yu School of Environment and Energy Peking University Shenzhen Graduate SchoolShenzhen518055China AI for Science(AI4S)-Preferred Program Peking UniversityShenzhen518055China Southern University of Sciences and Technology Yantian Hospital Shenzhen518081China Institute of Biomedicine and Biotechnology Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China State Key Laboratory of Isotope Geochemistry CAS Center for Excellence in Deep Earth ScienceGuangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou510640China School of Atmospheric Sciences Sun Yat-sen UniversitySouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai519082China Department of Civil Engineering University of Hong Kong999077Hong KongChina Department of Civil and Environmental Engineering Faculty of EngineeringUniversity of AucklandNew Zealand

Biosynthesis and biodegradation of microorganisms critically underpin the development of biotechnology,new drugs and therapies,and environmental ***,most uncultured microbial species along with their metabolic capacities in extreme environments,remain *** we unravel the metabolic potential of microbial dark matters(MDMs)in four deep-inland hypersaline lakes in Xinjiang,*** metagenomic binning,we uncovered a rich diversity of 3030 metagenomeassembled genomes(MAGs)across 82 phyla,revealing a substantial portion,2363 MAGs,as previously unclassified at the genus *** unknown MAGs displayed unique distribution patterns across different lakes,indicating a strong correlation with varied physicochemical *** analysis revealed an extensive array of 9635 biosynthesis gene clusters(BGCs),with a remarkable 9403 being novel,suggesting untapped biotechnological ***,some MAGs from potentially new phyla exhibited a high density of these *** biosynthesis,our study also identified novel biodegradation pathways,including dehalogenation,anaerobic ammonium oxidation(Anammox),and degradation of polycyclic aromatic hydrocarbons(PAHs)and plastics,in previously unknown microbial *** findings significantly enrich our understanding of biosynthesis and biodegradation processes and open new avenues for biotechnological innovation,emphasizing the untapped potential of microbial diversity in hypersaline environments.

关键词： Biosynthesis Biodegradation Hypersaline lakes Metagenome-assembled genomes Microbial dark matters

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Enhanced bioavailability and improved antimicrobial, antibiofilm, and antivirulence activities of fish gelatin-based nanoformulations prepared by coating of maltol-gold nanoparticles

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Chemosphere 2025年 379卷

作者： Tabassum, Nazia Khan, Fazlurrahman Jeong, Geum-Jae Oh, Do Kyung Kim, Young-Mog Marine Integrated Biomedical Technology Center The National Key Research Institutes in Universities Pukyong National University Busan48513 Korea Republic of Research Center for Marine Integrated Bionics Technology Pukyong National University Busan48513 Korea Republic of Ocean and Fisheries Development International Cooperation Institute Pukyong National University Busan48513 Korea Republic of International Graduate Program of Fisheries Science Pukyong National University Busan48513 Korea Republic of Interdisciplinary Program of Marine and Fisheries Sciences and Convergent Technology Pukyong National University Busan48513 Korea Republic of Department of Food Science and Technology Pukyong National University Busan48513 Korea Republic of

Biofilm formation by a single and multiple microbial species poses a significant challenge to healthcare due to biofilm-related antibiotic resistance. This study aimed to develop a nanoformulation (Mal-AuNP-Gel) by synthesizing gold nanoparticles (AuNPs) with maltol (Mal), and coating them with fish gelatin (Gel) to reduce biofilm formation and virulence characteristics of microbial pathogens. Mal-AuNP-Gel showed increased antibacterial activity against all pathogens studied, including bacterial (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Listeria monocytogenes, Escherichia coli, and methicillin-resistant S. aureus) and fungal pathogens (e.g., Candida albicans), with MICs up to 2-fold lower than those of Mal-AuNPs. At the sub-MIC level, Mal-AuNPs-Gel, compared to Mal-AuNPs, improved the inhibition of initial-stage biofilm development by a single species of P. aeruginosa, S. aureus, and C. albicans as well as the mixed-species biofilm of S. aureus and C. albicans. Additionally, these nanoparticles significantly inhibited several virulence characteristics such as hemolysis, pyoverdine and pyocyanin production, protease activity, and motility of P. aeruginosa. Furthermore, the expression of genes associated with biofilm formation, quorum sensing, motility, and virulence factors in P. aeruginosa was found to be suppressed by Mal-AuNPs-Gel at a higher level than that of Mal-AuNPs, corroborating the phenotypic effects. The non-cytotoxic effects of Mal-AuNPs and Mal-AuNPs-Gel at sub-MIC levels, as evidenced by results of in vitro cell cytotoxicity and in vivo phytotoxicity tests, further indicated biocompatibility of the synthesized nanoparticles. © 2025 Elsevier Ltd

关键词： Gold coatings

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The impact of copper oxide nanoparticles reinforced nanocellulose acetate membrane on antibacterial and dye removal Potency

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Results in Engineering 2024年 23卷

作者： Yanuhar, Uun Suryanto, Heru Amin, Muhamad Maulana, Jibril Mohamad Lal, Mohammad Tamrin Caesar, Nico Rahman Study Program of Aquatic Resources Management Faculty of Fisheries and Marine Sciences Universitas Brawijaya East Java Indonesia Department of Mechanical and Industrial Engineering Faculty of Engineering Universitas Negeri Malang Jl. Semarang 5 Malang65145 Indonesia Department of Aquaculture Faculty of Fisheries and Marine Universitas Airlangga Jl. Dharmahusada Permai Surabaya60115 Indonesia Borneo Marine Research Institute Universiti Malaysia Sabah Jalan UMS Sabah Kota Kinabalu88400 Malaysia Doctoral Program of Environment Science Post Graduate Program Universitas Brawijaya Jalan Veteran East Java Malang65145 Indonesia

With the rapid growth of the textile, leather, cosmetics, and pharmaceutical industries, concerns about pollutants in these sectors, especially dye pollutants, are increasing. This research characterizes bacterial nanocellulose acetate membranes made from Sargassum extract reinforced with copper oxide nanoparticles and tests their antibacterial properties and dye removal in water. Sargassum sp. extract was fermented using Acetobacter xylinum for 10 days, producing bacterial nanocellulose, which was then esterified to create bacterial nanocellulose acetate. Copper oxide nanoparticles were added to the bacterial nanocellulose acetate solution in varying concentrations, cast, and oven-dried. The membrane was analyzed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscope, antibacterial activity, and dye removal. Results showed uniform copper oxide nanoparticles distribution on the membrane surface. Structure analysis revealed diffraction peaks at 35.44° and 38.57°, indicating a reduced crystalline index but improved membrane effectiveness. Antibacterial tests showed a zone of inhibition against bacterial growth, increasing with higher copper oxide nanoparticle content. The addition of copper oxide nanoparticles also influenced the dye removal capacity for Metanil Yellow, Congo Red, Eosin Yellow, Methylene Blue, and Malachite Green. The kinetic analysis showed that the adsorption process follows a pseudo-first-order model, with higher correlation coefficients (R2) compared to the pseudo-second-order model. © 2024 The Author(s)

关键词： Fourier transform infrared spectroscopy

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Quantification of Litter in Cities Using a Smartphone Application and Citizen science in Conjunction with Deep Learning-Based Image Processing

SSRN

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SSRN 2023年

作者： Kako, Shin’ichiro Muroya, Ryunosuke Matsuoka, Daisuke Isobe, Atsuhiko Graduate School of Science and Engineering Department of Engineering Ocean Civil Engineering Program Kagoshima University Japan Japan Agency for Marine-Earth Science and Technology Japan Research Institute for Applied Mechanics Kyushu University Japan

We aimed to classify and quantify the abundance of litter in cities, which is a major source of marine plastic pollution, using citizen science combined with deep learning-based image processing. The project to collect image data including street litter through the smartphone application Pirika was launched in May 2018 and is ongoing and the total number of submitted images has reached one million. Of these, it was visually confirmed that images with clearly distinguished street litter accounted for approximately 30% of all images. Visual classification revealed that the most common types of litter were cans, followed by plastic bags, plastic bottles, cigarette butts, cigarette boxes, and masks. The top six categories account for approximately 80% of the total and the top three categories account for more than 60% of the total imaged litter. Based on these results, a classifier capable of classifying and quantifying these six litter types was constructed using image processing based on deep learning trained using the submitted images. This model was validated using images unused for training. The results revealed that both precision and recall derived from our model were higher than 75%. The quantity of litter derived from automated image processing was also plotted on a map using location data acquired concurrently with the images by the smartphone application. Therefore, we demonstrated that citizen science supported by smartphone applications and deep learning-based image processing is capable of visualizing/quantifying street litter in cities. © 2023, The Authors. All rights reserved.

关键词： Object detection

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Development of polyethyleneimine cross-linked fucoidan nanoparticles as delivery systems for improved anticancer efficiency of cytarabine in breast adenocarcinoma cell lines †

RSC Pharmaceutics

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RSC Pharmaceutics 2024年第2期1卷 305-316页

作者： Deepa Geethakumari Santhini Pulikkal Veettil Sivakumar Krishnankutty Nair Chandrika Anoop Bhaskaran Sathyabhama Rojin Joseph Shibin Sobhanam Padmini Jisha V. Somasekharan Sajeevan Thavarool Puthiyedathu National Centre for Aquatic Animal Health Cochin University of Science and Technology Cochin Kerala-16 India Rajiv Gandhi Centre for Biotechnology Poojappura Thiruvananthapuram Kerala-695014 India Research and Post Graduate Department of Chemistry MES Keveeyam College Valanchery-676552 Kerala India Department of Applied Chemistry Cochin University of Science and Technology Cochin Kerala-682022 India Department of Marine Biology Microbiology and Biochemistry Cochin University of Science and Technology Cochin Kerala-16 India sajeev@cusat.ac.in +91484 2381120 +91-***

Cytarabine, generally used for the treatment of haematological malignancies, has minimal activity in solid tumours. The present work focuses on the evaluation of the cytotoxic efficiency of cytarabine in MCF-7 cell lines with the aid of fucoidan nanoparticles as drug delivery systems. Polyethyleneimine (PEI) crosslinked fucoidan nanoparticles were synthesised by polyelectrolyte complexation and were characterised by FTIR and 1 H NMR. TEM analysis revealed cytarabine-loaded fucoidan nanoparticles (CFNPs) with a size of less than 40 nm. In vitro release kinetics studies showed that the release of cytarabine (82.17 ± 1.24%) from CFNPs was higher at pH 6.4. Molecular simulation studies revealed that fucoidan–cytarabine binding is mainly facilitated by hydrogen bonds. Internalisation of fucoidan nanoparticles by MCF-7 cells was tracked using the fluorophore, SQ 650. Cell viability studies showed improved cytotoxicity in CFNP-treated MCF-7 cell lines compared to free cytarabine. Quantitative real-time PCR showed upregulation of the expression of apoptotic genes, bax , cyt c and cas 9 in cells treated with CFNPs. Flow cytometry using Annexin V/PI displayed an increased percentage of apoptotic cells on treatment with CFNPs compared to cytarabine alone. The result of this study shows that the cytotoxic efficiency of cytarabine in MCF-7 cells can be enhanced using fucoidan nanoparticles as delivery systems.

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Nobiletin derivative, 5-acetoxy-6,7,8,3’,4’-pentamethoxyflavone, inhibits neuroinflammation through the inhibition of TLR4/MyD88/MAPK signaling pathways and STAT3 in microglia

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Immunopharmacology and Immunotoxicology 2024年第4期46卷 450-460页

作者： Chuang, Jimmy Ming-Jung Chen, Hsien-Lin Chang, Chi-I Lin, Jia-Syuan Chang, Hui-Min Wu, Wan-Ju Lin, Mei-Ying Chen, Wu-Fu Lee, Chien-Hsing Department of Neurosurgery Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine Kaohsiung Taiwan Division of General Surgery Department of Surgery Chi Mei Medical Center Tainan Liouying Taiwan Department of Biological Science and Technology National Pingtung University of Science and Technology Research Centre for Active Natural Products Development National Pingtung University of Science and Technology Pingtung Taiwan Department of Pharmacology School of Post-Baccalaureate Medicine Division of Pharmacology and Traditional Chinese Medicine Graduate Institute of Medicine College of Medicine Kaohsiung Medical University Kaohsiung Taiwan Community Health Promotion Center Kaohsiung Municipal Ci-Jin Hospital Kaohsiung Taiwan Department of Marine Biotechnology and Resources National Sun Yat-Sen University Kaohsiung Taiwan Department of Medical Research Kaohsiung Medical University Hospital Kaohsiung Taiwan Drug Development and Value Creation Research Center Kaohsiung Medical University Kaohsiung Taiwan

Objective: Microglia in the central nervous system regulate neuroinflammation that leads to a wide range of neuropathological alterations. The present study investigated the anti-neuroinflammatory properties of nobiletin (Nob) derivative, 5-acetoxy-6,7,8,3′,4′-pentamethoxyflavone (5-Ac-Nob), in lipopolysaccharide (LPS)-activated BV2 microglia. Materials and Methods: By using the MTT assay, Griess method, flow cytometry, and enzyme-linked immunosorbent assay (ELISA), we determined the cell viability, the levels of nitric oxide (NO), reactive oxygen species (ROS), and pro-inflammatory factors (interleukin 1 beta;IL-1β, interleukin 6;IL-6, tumor necrosis factor alpha;TNF-α and prostaglandin E2;PGE2) in LPS-stimulated BV2 microglia. Toll-like receptor 4 (TLR4)-mediated myeloid differentiation primary response gene 88 (MyD88)/nuclear factor-kappa B (NF-κB), mitogen-activated protein kinase (MAPK) signaling pathway and signal transducer and activator of transcription 3 (STAT3) were measured by western blotting. Analysis of NO generation and mRNA of pro-inflammatory cytokines was confirmed in the zebrafish model. Results: 5-Ac-Nob reduced cell death, the levels of NO, ROS, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), and pro-inflammatory factors in LPS-activated BV-2 microglial cells. TLR4-mediated MyD88/NF-κB and MAPK pathway (p38, ERK and JNK) after exposure to 5-Ac-Nob was also suppressed. Moreover, 5-Ac-Nob inhibited phosphorylated STAT3 proteins expression in LPS-induced BV-2 microglial cells. Furthermore, we confirmed that 5-Ac-Nob decreased LPS-induced NO generation and mRNA of pro-inflammatory cytokines in the zebrafish model. Conclusions: Our findings suggest that 5-Ac-Nob represses neuroinflammatory responses by inhibiting TLR4-mediated signaling pathway and STAT3. As a result of these findings, 5-Ac-Nob has potential as an anti-inflammatory agent against microglia-mediated neuroinflammatory disorders. © 2024 Informa UK Limited, trading as Ta

关键词： 5-Acetoxy-6,7,8,3′,4′-pentamethoxyflavone microglia-mediated neuroinflammatory disorders NF-κB/IκB STAT3 TLR4/MyD88/MAPK

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An Efficient Three-Factor Authenticated Key Agreement Technique Using FCM Under HC-IoT Architectures

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Computers, Materials & Continua 2022年第7期72卷 1373-1389页

作者： Chandrashekhar Meshram Agbotiname Lucky Imoize Sajjad Shaukat Jamal Parkash Tambare Adel R.Alharbi Iqtadar Hussain Department of Post Graduate Studies and Research in Mathematics Jayawanti Haksar Government Post-Graduation CollegeCollege of Chhindwara UniversityBetul460001M.P.India Department of Electrical and Electronics Engineering Faculty of EngineeringUniversity of LagosAkokaLagos100213Nigeria Department of Electrical Engineering and Information Technology Institute of Digital CommunicationRuhr University44801BochumGermany Department of Mathematics College of ScienceKing Khalid UniversityAbhaSaudi Arabia Water Resources&Applied Mathematics Research Lab Nagpur440027India College of Computing and Information Technology University of TabukTabuk71491Saudi Arabia Mathematics Program Department of MathematicsStatistics and PhysicsCollege of Arts and SciencesQatar University2713DohaQatar

The Human-Centered Internet of Things(HC-IoT)is fast becoming a hotbed of security and privacy *** users can establish a common session key through a trusted server over an open communication channel using a three-party authenticated key *** of the early authenticated key agreement systems relied on pairing,hashing,or modular exponentiation processes that are computationally intensive and *** order to address this problem,this paper offers a new three-party authenticated key agreement technique based on fractional chaotic *** new scheme uses fractional chaotic maps and supports the dynamic sensing of HC-IoT devices in the network architecture without a password *** projected security scheme utilized a hash function,which works well for the resource-limited HC-IoT *** results show that our new technique is resistant to password guessing attacks since it does not use a ***,our approach provides users with comprehensive privacy protection,ensuring that a user forgery attack causes no ***,our new technique offers better security features than the techniques currently available in the literature.

关键词： Three-party authenticated key agreement anonymity fractional chaotic maps Chebyshev polynomial password table human-centered internet of things(HC-IoT)

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GIS-based spatiooral analysis on Yellow Fin Tuna catch in Eastern Indian Ocean off Sumatera 3

GIS-based spatiooral analysis on Yellow Fin Tuna catch in Ea...

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3rd International Conference on marine science: Towards Sustainable marine Resources and Environment, ICMS 2019

作者： Nurholis Gaol, J.L. Syah, A.F. Dewi, A.K. Graduate School of Marine Technology Program IPB University Bogor Indonesia Department of Marine Science and Technology IPB University Bogor Indonesia Marine Science Study Program Faculty of Agriculture Trunojoyo University Madura Indonesia

Spatiooral analysis of yellow fin tuna fishing activity could give us new perception and perspective on studying this fisheries resource exploration. This study was carried out in Eastern Indian Ocean off Sumatera. Fishing data were collected between 2014 until 2018 from the hand line fishermen's daily logbooks accessed from Bungus fishing port. Data were organized into a database and structured on a geographical reference to allow GIS-Based analysis. We performed raster calculator analysis and spatial statistical analysis to understand spatiotemporal distribution behaviour and fishing activity also employed the generalized additive model to understand the habitat preferences. The result of GIS-based analysis shows the dynamics of catch, effort and catch per unit effort distribution patterns, underlining the annual differences of geographical distribution and fishing pattern. The most concentrated fishing activity monitored in 2014 while the more dispersed fishing activity monitored in 2017. The geographic orientation of spatial distribution monitored at range between 72.90 degrees until 176.15 degrees. GIS provide an important and powerful tool to analyse fishing information to help decision makers in the EIO off Sumatera on Tuna management. © Published under licence by IOP Publishing Ltd.

关键词： Geographic information systems

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Transmission of Rossby Waves Through the Philippine Archipelago

SSRN

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SSRN 2023年

作者： Malugao, Mac Euan D. Jan, Sen Chang, Ming-Huei Ho, Tung-Yuan Yang, Yiing Jang Earth System Science Program Taiwan International Graduate Program Academia Sinica Taipei115201 Taiwan Taiwan International Graduate Program for Earth System Science National Taiwan University Taipei10617 Taiwan Institute of Oceanography National Taiwan University Taipei10617 Taiwan Research Center for Environmental Changes Academia Sinica Taipei115201 Taiwan Department of Marine Science College of Science and Mathematics Mindanao State University Iligan Institute of Technology Iligan City9200 Philippines

Oceanic Rossby waves play a crucial role in shaping the physical and biological dynamics of both open and coastal oceans, especially within the tropical band spanning between the 10°S and 10°N parallels. Yet, the extent to which Rossby waves can transmit and impact the hydrography and ecosystem of semi-enclosed seas like the South China Sea (SCS) remains unclear. This study aims to investigate the transmission of Rossby waves through the Philippine archipelago, using satellite altimeter-derived sea level anomaly (SLA) and coastal tide gauge records. Our findings reveal that westward-propagating Rossby waves in the tropical Pacific Ocean with a wave speed of ~0.64 m s−1 first entered the Celebes Sea, and then passed through the Sibutu Passage into the Sulu Sea from April to December 2017. Subsequently, the waves propagated along the northeast coast of Sabah and the east coast of Palawan before exiting through the Mindoro Strait to the central SCS. Additionally, a β-refracted Rossby wave with wave speed of 0.28 m s−1 also penetrated the archipelago but at a higher latitude from July to November via Surigao Strait and propagated toward the south and north of Palawan, ultimately reaching the west coast of Palawan in the eastern central SCS. © 2023, The Authors. All rights reserved.

关键词： Sea level

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Capnesterones A and B, new steroids isolated from the soft coral Capnella imbricata

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Phytochemistry Letters 2024年 61卷 115-119页

作者： Cheng, Ting-Yu Yang, Chia-Jung Chen, Po-Jen Chen, Yu-Li Peng, Bo-Rong Kung, Te-An Wen, Zhi-Hong Lai, Kuei-Hung Chung, Hsu-Ming Department of Applied Chemistry National Pingtung University Pingtung 900393 Taiwan Clinical Immunology Laboratory Department of Medical Research E-Da Hospital I-Shou University Kaohsiung 824005 Taiwan Research Center for Chinese Herbal Medicine Graduate Institute of Healthy Industry Technology College of Human Ecology Chang Gung University of Science and Technology Taoyuan 33305 Taiwan Graduate Institute of Biomedical Sciences Graduate Institute of Natural Products College of Medicine Chang Gung University Taoyuan 33305 Taiwan Graduate Institute of Pharmacognosy College of Pharmacy Taipei Medical University Taipei 11031 Taiwan Institute of Food Safety Management National Pingtung University of Science and Technology Pingtung 91201 Taiwan Department of Marine Biotechnology and Resources National Sun Yat-sen University Kaohsiung 804201 Taiwan Institute of BioPharmaceutical Sciences National Sun Yat-sen University Kaohsiung 804201 Taiwan PhD Program in Clinical Drug Development of Herbal Medicine College of Pharmacy Taipei Medical University Taipei 11031 Taiwan Traditional Herbal Medicine Research Center Taipei Medical University Hospital Taipei 11031 Taiwan

Two new non-withanolidal steroids with a cross-conjugated dienone structural unit in ring A, capnesterones A (1) and B (2), were isolated from the soft coral Capnella imbricata in the southeast waters of Taiwan. The compounds' structures were elucidated through a comprehensive analysis of spectroscopic data, including IR, ESIMS, ¹H NMR, and ¹³C NMR, and through comparison with previous literature. At a concentration of 10 μM, steroids 1 and 2 exhibited reductions in iNOS levels (60 % and 82 %) compared to LPS-stimulated control cells, along with increased release (134 % and 110 %) against COX-2 protein expressions. © 2024 Phytochemical Society of Europe

关键词： Capnella imbricata COX-2 INOS Steroid

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