Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with s...
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Colorectal cancer (CRC) ranks among the most prevalent tumors, particularly affecting men and women, with mouse models and imaging technologies like colonoscopy, endoluminal (eUBM), and transabdominal (tUBM) ultrasoun...
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ISBN:
(数字)9798350349085
ISBN:
(纸本)9798350349092
Colorectal cancer (CRC) ranks among the most prevalent tumors, particularly affecting men and women, with mouse models and imaging technologies like colonoscopy, endoluminal (eUBM), and transabdominal (tUBM) ultrasound biomicroscopy playing pivotal roles in exploring new treatment avenues. The evaluation of colon wall thickness (Wt), a significant marker of tumor presence, is feasible through eUBM or tUBM, contrasting with colonoscopy's limitations. This study scrutinizes the consistency of Wt measurements obtained via eUBM and tUBM in mice, with or without colon tumors induced by azoxymethane (AOM) and dextran sulfate sodium salt (DSS). UBM images were captured using both eUBM and tUBM instruments, with subsequent Wt measurements performed using ImageJ software. Comparison between Wt obtained from eUBM and tUBM images used the One-Way ANOVA, for normal distributed data, or the Kruskal-Wallis test otherwise. Local Ethics Committee for Animal Use in Research approved the work (protocol 014/20). In general, the results indicate a substantial median Wt disparity between control and tumor groups measured by both techniques (p<0.05). Notably, discrepancies in Wt findings between the two methods were observed in the control group (p<0.05), despite the excellent resolution of the images in delineating colon layers.
Gas vesicles (GVs), gas-filled protein-encapsulated nanostructures, synthesized by microorganisms such as Halobacterium salinarum NRC-1 (HALO) and Anabaena flos aquae (ANA) have been reported as potential ultrasound c...
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ISBN:
(数字)9798350349085
ISBN:
(纸本)9798350349092
Gas vesicles (GVs), gas-filled protein-encapsulated nanostructures, synthesized by microorganisms such as Halobacterium salinarum NRC-1 (HALO) and Anabaena flos aquae (ANA) have been reported as potential ultrasound contrast agents (UCA) due to their ability to mechanically vibrate with the same frequency, or harmonics, as the one from the incident ultrasound (US) wave. Despite GVs resonating in frequencies above 21 MHz, they collapse irreversibly when the US incident field pressure exceeds the vesicle collapse pressure. There are few reports on the relationship between the pressure of the acoustic wave incident in the GVs and the corresponding intensity of the backscattered wave signal from the GVs and, therefore, this study was designed to investigate how varying the relative incident US power, at 21MHz, reflects on the intensity of the US backscattered signal and the collapse of HALO and ANA GVs. The mean (±SD) of the grey level intensity for the ROIs, in maximum intensity pixel images, with GVs and PBS, were determined for transmitted acoustic relative power of 1, 5, 10, 20%. ANA-GVs presented a lower signal intensity, but greater resistance to the increase in relative US pressure. In conclusion, the best behavior was for the Halo-GVs at 5% power and at 21MHz frequency.
This article proposes the creation, implementation and development of an integration plugin for ImageJ that makes calls in MATLAB functions for the segmentation UBM imagens, more specific endoluminal (eUBM) e transabd...
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ISBN:
(数字)9798350349085
ISBN:
(纸本)9798350349092
This article proposes the creation, implementation and development of an integration plugin for ImageJ that makes calls in MATLAB functions for the segmentation UBM imagens, more specific endoluminal (eUBM) e transabdominal (tUBM), in the laboratory the images are generating with frequencies between 30-40 MHZ. These images allow view colon layer, identify tumors in colons, to calculate tumor volume and 3D visualization. This integration may to improve the time taken to perform the procedure and the user experience to reduce the number of tasks and change windows, making the experience more fluid and with less risk of errors.
Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with s...
Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with specific molecules in tumor microenvironment. An important target of investigation is Cathepsin B (CTSB), an enzyme produced by several tumors. CTSB acts in the tumoral microenvironment to increase tumor growth and invasion. This work objective relied on bioengineering the GVs to promote the GvpC-CTSB interaction with a subsequent increase of ultrasound (US) signal backscattered. GvpC was engineered to contain a known CTSB cleavage sequence re-added in striped GV (ΔGV). Western Blot (WB) test was used to compare the GVwt (wild type) and GV* (GV engendered) before and after 24 hours reaction with CTSB. US images for PBS (negative control), GVtw, ΔGV, GV* and CTSB+GV*, were obtained in 21MHz (5% total power) and regions-of-interest (ROIs) over the final B-mode image were determined. The WB test for GVwt resulted in mean (±SD) GvpC concentrations of 25169 (±8574) and 32873 (±9113) and GV* was 32487 (±6867) and 12763 (±2846), at t0 and t24, respectively. The US image gray level intensities for the ROIs related to the samples of PBS, GVwt, ΔGV, GV* and CTSB+GV* were 55.8 (±17.6), 47.4 (±16.6), 46.1 (±22.8) and 150.2 (±24), respectively. These results reveal an increase in the US signal backscattered from GV* after CTSV reaction. This increase is likely related to a direct GvpC degradation by CTSB, without affecting the GV* structure. In conclusion, high frequency US B-mode images showed the GV* with the potential to act as an UCA sensitive to the CTSB presence in tumoral microenvironment.
Colon cancer has a high incidence, without considering skin cancer, being the third most common cancer in the world. Despite this high incidence and high mortality rate, most of the outcomes could be prevented by usin...
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ISBN:
(数字)9781728154480
ISBN:
(纸本)9781728154497
Colon cancer has a high incidence, without considering skin cancer, being the third most common cancer in the world. Despite this high incidence and high mortality rate, most of the outcomes could be prevented by using accurate techniques for early cancer detection to enable early treatment. In this context, it is important to conduct preclinical studies, using animal models of colon diseases, to understand the development of the disease and to test new diagnostic approaches including imaging instrumentation. We have previously used a 3-French ultrasound mini-probe to image the layered structure in the mouse colon, to detect polyps, and to visualize their invasion through the colon wall. The present work aimed to measure the colon wall thickness and perform a numeric differentiation between the thickness of normal and inflamed mouse colon, a crucial step to elucidate the disease progression in colorectal cancer mouse model.
B Correction to: Clinical Oral Investigations b https://***/10.1007/s00784-022-04735-z The affiliations of Dr. Horsophonphong and Dr. Bertassoni were published incorrectly: Sivaporn Horsophonphong SP 5,6 sp Luiz Berta...
B Correction to: Clinical Oral Investigations b https://***/10.1007/s00784-022-04735-z The affiliations of Dr. Horsophonphong and Dr. Bertassoni were published incorrectly: Sivaporn Horsophonphong SP 5,6 sp Luiz Bertassoni SP 8,9,10,11,12 sp Affiliations: SP 5 sp Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Salaya, Thailand SP 6 sp Department of Orthodontics, School of Medicine, School of engineering, Tufts University, Boston, MA, 02,111, USA SP 8 sp Department of Orthodontics, School of Medicine, School of engineering, Tufts University, Boston, MA, 02,111, USA SP 9 sp Center for Regenerative Medicine, School of Medicine, Oregon Health & Science University, Portland, OR, USA SP 10 sp Department of biomedicalengineering, School of Medicine, Oregon Health & Science University, Portland, OR, USA SP 11 sp Cancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Portland, OR, USA SP 12 sp Biomaterials and Biomechanics, School of Dentistry, OR Health & Science University- OHSU, 2730 S.W. Moody Ave, Portland, OR, 97,201, USA B The affiliations should be corrected as follows: b Sivaporn Horsophonphong SP 5 sp Luiz Bertassoni SP 4,8,9,10 sp Affiliations SP 4 sp Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health & Science University, Portland, OR, USA SP 5 sp Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Salaya, Thailand SP 8 sp Center for Regenerative Medicine, School of Medicine, Oregon Health & Science University, Portland, OR, USA SP 9 sp Department of biomedicalengineering, School of Medicine, Oregon Health & Science University, Portland, OR, USA SP 10 sp Cancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Portland, OR, USA The authors apologize for any inconvenience that this mistake may have caused. [Extracted from the article]
Objectives Some pro-inflammatory lipids derived from 1 lipooxygenase enzyme are potent neutrophil chemoattractant, a cell centrally involved in acute respiratory distress syndrome (ARDS); a syndrome lacking effective ...
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Objectives Some pro-inflammatory lipids derived from 1 lipooxygenase enzyme are potent neutrophil chemoattractant, a cell centrally involved in acute respiratory distress syndrome (ARDS); a syndrome lacking effective treatment. Considering the beneficial effects of the leukotriene receptor inhibitor, montelukast, on other lung diseases, whether montelukast attenuates inflammation in a mouse model of ARDS, and whether it reduces LPS stimulated activation of human neutrophils was investigated. Methods Thirty-five C57Bl/6 mice were distributed into control (PBS) + 24 h, LPS + 24 h (10 μg/mouse), control + 48 h, LPS + 48 h, and LPS 48 h + Montelukast (10 mg/kg). In addition, human neutrophils were incubated with LPS (1 μg/mL) and treated with montelukast (10 μM). Results Oral-tracheal administration of montelukast significantly attenuated total cells ( P < .05), macrophages ( P < .05), neutrophils ( P < .01), lymphocytes ( P < .001) and total protein levels in BAL ( P < .05), as well as IL-6 ( P < .05), CXCL1/KC ( P < .05), IL-17 ( P < .05) and TNF-α ( P < .05). Furthermore, montelukast reduced neutrophils ( P < .001), lymphocytes ( P < .01) and macrophages ( P < .01) in the lung parenchyma. In addition, montelukast restored BAL VEGF levels ( P < .05). LTB4 receptor expression ( P < .001) as well as NF-κB ( P < .001), a downstream target of LPS, were also reduced in lung parenchymal leukocytes. Furthermore, montelukast reduced IL-8 ( P < .001) production by LPS-treated human neutrophils. Conclusion In conclusion, montelukast efficiently attenuated both LPS-induced lung inflammation in a mouse model of ARDS and in LPS challenged human neutrophils. Objetivos Algunos lípidos proinflamatorios derivados de la enzima lipooxigenasa 1 son potentes quimioatrayentes de neutrófilos, un tipo celular con una implicación principal en el síndrome de distrés respiratorio agudo (SDRA),
Blood clotting, a process to avoid bleeding, involves vasoconstriction, platelet plug build-up, coagulation, clot retraction and fibrinolysis. During coagulation, blood changes from liquid to gel, and the clot viscoel...
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